KEGG ENZYME: 1.3.1.85

ENZYME: 1.3.1.85

Entry

EC 1.3.1.85                 Enzyme

Name

crotonyl-CoA carboxylase/reductase;
CCR;
crotonyl-CoA reductase (carboxylating)

Class

Oxidoreductases;
Acting on the CH-CH group of donors;
With NAD+ or NADP+ as acceptor

Sysname

(2S)-ethylmalonyl-CoA:NADP+ oxidoreductase (decarboxylating)

Reaction(IUBMB)

(2S)-ethylmalonyl-CoA + NADP+ = (E)-but-2-enoyl-CoA + CO2 + NADPH + H+ [RN:R09291]

Reaction(KEGG)

R09291

Substrate

(2S)-ethylmalonyl-CoA [CPD:C18026];
NADP+ [CPD:C00006]

Product

(E)-but-2-enoyl-CoA [CPD:C00877];
CO2 [CPD:C00011];
NADPH [CPD:C00005];
H+ [CPD:C00080]

Comment

The reaction is catalysed in the reverse direction. This enzyme, isolated from the bacterium Rhodobacter sphaeroides, catalyses (E)-but-2-enoyl-CoA-dependent oxidation of NADPH in the presence of CO2. When CO2 is absent, the enzyme catalyses the reduction of (E)-but-2-enoyl-CoA to butanoyl-CoA, but with only 10% of maximal activity (relative to (E)-but-2-enoyl-CoA carboxylation).

History

EC 1.3.1.85 created 2011

Pathway

ec00630

Glyoxylate and dicarboxylate metabolism

ec01100

Metabolic pathways

ec01120

Microbial metabolism in diverse environments

Orthology

K14446

crotonyl-CoA carboxylase/reductase

Genes

ECA:	ECA0600(cfa8B)

PATR:

EV46_04860

PATO:

GZ59_09850(cfa8B)

PACB:

M9782_06870(ccrA)

DIC:	Dpoa569_002944(ccrA)

BNG:	EH206_19780(ccrA)

LBQ:	CKQ53_08765(ccrA)

PAY:

PAU_02213

PST:	PSPTO_4689(cfa8)

PAMG:

BKM19_030840(ccrA)

PCAB:

JGS08_03260(ccrA)

PCOF:

POR16_03640(ccrA)

PTRE:

I9H09_26050(ccrA)

PPV:	NJ69_15265

PIZ:	LAB08_R41860(ccrA)

ALKM:

NKI27_11405(ccrA)

ASEM:

NNL22_07760(ccrA)

ZPL:	ZBT109_0360

OME:	OLMES_2177

BVE:	AK36_5943(ccrA)

BAM:

Bamb_6221

BCON:

NL30_35040

BARI:

NLX30_36090(ccrA)

BGP:	BGL_1c19630

BPLA:

bpln_1g17940

PARA:

BTO02_25445

PEW:	KZJ38_27745(ccrA)

JAJ:	EKL02_10350(ccrA)

APP:	CAP2UW1_2501

PLA:

Plav_1621

RBS:	RHODOSMS8_02646(ccrA2)

BGQ:	X265_36830(ccrA)

BGZ:	XH91_36035(ccrA)

BVZ:	BRAD3257_7532(ccr)

BAUT:

QA635_32920(ccrA)

BOP:	AXW83_03430

BOS:	BSY19_2139(ccrA)

BVV:	BHK69_16350

BOI:	BLM15_10545

BOF:	FQV39_08895(ccrA)

BVES:

QO058_26715(ccrA)

XAU:

Xaut_1195

XDI:	EZH22_01060(ccrA)

AZC:

AZC_0997

MEA:	Mex_1p0178(ccr)

MDI:	METDI0161(ccr)

MEX:

Mext_0288

MCH:

Mchl_0332

MPO:

Mpop_0364

MRD:	Mrad2831_1105

MET:	M446_0037 M446_5660

MNO:

Mnod_0039

MOR:	MOC_1328(ccrA)

META:

Y590_00895

MAQU:

Maq22A_1p32000(ccrA)

MPHY:

MCBMB27_00696

MEE:	DA075_05075(ccrA)

METD:

C0214_01265(ccrA)

METX:

A3862_25070

METS:

DK389_18840(ccrA)

METI:

DK427_18535(ccrA)

MMES:

MMSR116_22775(ccrA)

MTEA:

DK419_07965(ccrA)

MIND:

mvi_52670(ccr)

MOG:	MMB17_10655(ccrA)

RHJ:	HZY79_04295(ccrA)

CHEL:

AL346_09685

CDQ:	BOQ54_02760

HDN:

Hden_0216

HDT:	HYPDE_23643

HMC:	HYPMC_0098(ccr)

HNI:	W911_17400

RVA:

Rvan_2964

RLAC:

QMO75_11170(ccrA)

FIL:	BN1229_v1_1373(ccr)

FIY:	BN1229_v1_1372(ccr)

RHZ:	RHPLAN_11780

MCG:

GL4_3337

METG:

HT051_03000(ccrA)

BVR:

BVIR_87

BLAG:

BLTE_34310

MSC:

BN69_1948

MBRY:

B1812_02315

MROS:

EHO51_03605(ccrA) EHO51_15500(ccrA) EHO51_19170(ccrA)

MHEY:

H2LOC_016875(ccrA)

MPAR:

F7D14_06270(ccrA)

MIWA:

SS37A_03170(ccr)

MTW:	CQW49_03790(ccrA)

CMET:

K6K41_13855(ccrA)

PLEO:

OHA_1_02831(ccrA)

HDI:	HDIA_3961(ccrA2)

PSIN:

CAK95_11885

NOH:	G5V57_10405(ccrA)

RBM:

TEF_03480

PSF:

PSE_0115

PPHR:

APZ00_07795

LAP:	ACP90_02770

LABR:

CHH27_17725(ccrA)

LABP:

FJ695_06475(ccrA)

LABT:

FIU93_08395(ccrA1)

LAGG:

B0E33_14250

SIW:	GH266_11590(ccrA)

PHR:	C6569_14500(ccrA)

PSTG:

E8M01_09065(ccrA)

CCR:

CC_3085

CCS:	CCNA_03181

CAK:

Caul_1067

CSE:

Cseg_3646

CHQ:	AQ619_02890

CMB:	CSW64_02210(ccrA)

CFH:	C1707_03615(ccrA)

CAUF:

CSW63_19590(ccrA)

TSV:	DSM104635_01214(ccrA2)

SIL:	SPO0370(ccrA)

SIT:	TM1040_3104

RUA:	D1823_12085(ccrA)

RUT:	FIU92_01410(ccrA1)

RCON:

K3740_01540(ccrA)

JAN:

Jann_0793

RDE:	RD1_1637(ccrA)

RLI:	RLO149_c030710(ccr)

RPON:

G3256_15260(ccrA)

DSH:

Dshi_2853

PGA:	PGA1_c03870

PGL:	PGA2_c03420

PGD:

Gal_03115

PHP:	PhaeoP97_02969

PPIC:

PhaeoP14_00304

PHQ:	D1820_14795(ccrA)

OAT:	OAN307_c41350(ccr)

OAR:	OA238_c04430(ccr)

OTM:	OSB_04920(ccrA2)

OCT:	FTO60_13700(ccrA)

LMD:	METH_03410

LEJ:	ETW24_04250(ccrA)

LAQU:

R2C4_13535(ccrA)

LEV:	ETW23_15950(ccrA)

LCAE:

K3721_03535(ccrA)

RED:	roselon_01708

CID:

P73_3852

CMAR:

IMCC12053_3007

CEH:	CEW89_10025(ccrA)

MALG:

MALG_02723

CON:	TQ29_02510

YPAC:

CEW88_11095(ccrA)

YAN:	AYJ57_04475

TPRO:

Ga0080559_TMP1106

PABY:

Ga0080574_TMP3170

SUAM:

BOO69_13585

SULZ:

C1J03_18095(ccrA)

SULI:

C1J05_18020(ccrA)

SULD:

B5M07_05055

SPOT:

G6548_04225(ccrA)

SMED:

JNX03_18140(ccrA)

SINL:

DSM14862_02472(ccrA2_2)

SFAV:

PL335_11775(ccrA)

SPSE:

SULPSESMR1_00409(ccrA2)

TOM:	BWR18_01125

RMM:	ROSMUCSMR3_01832(ccrA2)

ROK:	RAK1035_1354

RID:	RIdsm_00116(ccrA2_1)

ROM:	EI983_03055(ccrA)

ROH:	FIU89_18780(ccrA2)

RPEL:

N7U68_09875(ccrA)

AHT:	ANTHELSMS3_02145(ccr)

SAGU:

CDO87_04300(ccrA)

THAA:

CFI11_20735(ccrA)

OCD:	FHY55_05955(ccrA)

MARU:

FIU81_04185(ccrA2)

ROT:	FIV09_01485(ccrA2)

RMH:	LVO79_01145(ccrA)

MALU:

KU6B_41650(ccr)

TGL:	HFZ77_08320(ccrA)

TMD:	KUV46_08220(ccrA)

HAT:	RC74_09425

PALX:

GQA70_04865(ccrA)

LSAL:

KBK07_01490(ccrA)

LALG:

LentiSH36_00624

TRY:	QF118_18585(ccrA)

RSP:

RSP_0960

RSH:	Rsph17029_2620

RSQ:	Rsph17025_3009

RSK:	RSKD131_2352

CAZT:

LV780_12820(ccrA)

RCP:	RCAP_rcc03243(ccrA)

RHP:	LPB142_14610

RBL:	B6K69_07515

PDE:

Pden_3873

PAMI:

JCM7686_1036

PYE:	A6J80_12555(ccrA)

PCON:

B0A89_06930

PZH:	CX676_11125(ccrA)

PARO:

CUV01_03100(ccrA)

PAMN:

JCM7685_1439

PMUT:

DPM13_11015(ccrA)

PARS:

DRW48_03195(ccrA)

PARR:

EOJ32_14935(ccrA)

PAAK:

FIU66_03190(ccrA)

PKD:	F8A10_08040(ccrA)

PPAN:

ESD82_06425(ccrA)

PLIA:

E4191_03280(ccrA)

PSAN:

HGN31_12030(ccrA)

PMAU:

CP157_00622(ccrA2)

PMEH:

JWJ88_03135(ccrA)

PSEW:

JHW44_03805(ccrA)

PSAP:

JHX88_04440(ccrA)

PFIS:

JHX87_08940(ccrA)

PAEX:

JHW48_12345(ccrA)

PSTL:

JHW45_04615(ccrA)

PALP:

JHW40_12250(ccrA)

PVER:

E3U25_03475(ccrA)

PTP:	RCA23_c09190(ccr)

RSU:

NHU_00739

RHM:	B5V46_04550

RHC:

RGUI_0609

RMB:	K529_020845

DAA:	AKL17_0912

DON:	BSK21_00885

RBZ:	B9057_00670

THW:	BMG03_18280

TEC:	AKL02_018635(ccrA)

LVS:	LOKVESSMR4R_02786(ccrA2)

RBG:	BG454_11885(ccrA)

GEH:	HYN69_12810(ccrA)

GFU:	KM031_04390(ccrA)

TAW:	EI545_06880(ccrA)

SALO:

EF888_18445(ccrA)

SEDI:

EBB79_02330(ccrA)

BOO:	E2K80_14180(ccrA)

PSEB:

EOK75_09460(ccrA)

PPRU:

FDP22_03530(ccrA)

PAED:

G5B38_14070(ccrA)

MON:	G8E03_02130(ccrA)

PAMO:

BAR1_15085(ccrA)

PSHQ:

F3W81_20350(ccrA)

POZ:	I0K15_10720(ccrA)

PALW:

PSAL_018850(ccr)

PGV:	SL003B_0355

CACT:

HZ995_03590(ccrA)

THAS:

C6Y53_10565(ccrA)

HDH:	G5B40_19100(ccrA)

CMAG:

CBW24_14080

GCE:	KYE46_04130(ccrA)

NSM:	JO391_13150(ccrA)

ACRO:

K3J57_10545(ccrA)

SINR:

O5O51_13235(ccrA)

AALK:

LGT41_0001985(ccrA)

AMYL:

QBD29_12720(ccrA)

AMAQ:

GO499_14275(ccrA)

RHOC:

QTA57_01975(ccrA)

PBAE:

P8S53_10200(ccrA)

FAQ:	G5B39_05195(ccrA)

HNE:	HNE_3118(ccrA)

HBA:

Hbal_0288

CBOT:

ATE48_04265

HBC:	AEM38_09265

SKT:	IGS68_10660(ccrA)

SMUC:

JL100_016570(ccrA)

SROE:

JL101_014995(ccrA)

RRU:

Rru_A3063

RRF:

F11_15690

RPM:	RSPPHO_00638

MAG:

amb0309

MGY:	MGMSRv2__2674

MGRY:

MSR1_36920(ccrA2)

MAGX:

XM1_1237(ccr)

MAGN:

WV31_14300

FER:	FNB15_00565(ccrA)

HTQ:	FRZ44_46290

HADH:

FRZ61_01130(adhP) FRZ61_44220

DEX:	HWD60_01570(ccrA)

DVN:	HQ394_15000(ccrA)

TMO:

TMO_0119

TXI:

TH3_17595

THAC:

CSC3H3_16685(ccrA)

TII:	DY252_04210(ccrA)

THAI:

IT893_16600(ccrA)

MAGQ:

MGMAQ_3100

VSU:	P3M64_11860(ccrA)

ECOG:

FIV45_15325(ccrA)

SNEA:

NBZ79_17900(ccrA)

CFUS:

CYFUS_003175

VIN:	AKJ08_0760

PSOP:

KP014_14685(ccrA)

CSR:	Cspa_c03820(ccr)

RCH:

RUM_00790

NTP:	CRH09_38255(ccrA)

ROP:

ROP_72100

RKO:	JWS14_45090(ccrA) JWS14_48900(ccrA)

SALB:

XNR_5889

SMA:	SAVERM_414(pteB)

SRC:	M271_43880

SBH:

SBI_01373

SAMB:

SAM23877_0426

SCX:	AS200_43230

STRF:

ASR50_01505

SKY:	D0C37_31705(ccrA)

SPHW:

NFX46_22155(ccrA)

SPLU:

LK06_031365

SPUN:

BFF78_04530

SLAU:

SLA_0406 SLA_7484

SRJ:

SRO_7228

SAST:

CD934_28935(ccrA)

SVN:	CP980_28480(ccrA)

SRK:	FGW37_32970(ccrA)

SGF:	HEP81_07556(ccrA_2)

SFUG:

CNQ36_01460(ccrA)

SPAC:

B1H29_00485 B1H29_36575

SATA:

C5746_38515(ccrA)

SMOB:

J7W19_12560(ccrA)

SPEU:

CGZ69_08265(ccrA) CGZ69_28875(ccrA)

SGJ:	IAG43_33070(ccrA)

SHK:	J2N69_01060(ccrA)

SDD:	D9753_11255(ccrA)

SACT:

DMT42_00480(ccrA)

SRUG:

F0345_01225(ccrA)

SAKB:

K1J60_45170(ccrA)

SVIO:

HWN34_29785(ccrA)

STEE:

F3L20_18785(ccrA)

AUS:	IPK37_16815(ccrA)

NMES:

H9L09_18030(ccrA)

NDA:

Ndas_0488

NAL:

B005_3356

NEX:	NE857_08735(ccrA)

NCG:	KGD84_25020(ccrA)

AORI:

SD37_26720

PSUU:

Psuf_066920(adhP_1) Psuf_073520(adhP_2)

BSOL:

FSW04_11630(ccrA)

BALA:

DSM104299_01927(ccr)

CWO:

Cwoe_4182

PARN:

NBH00_07305(ccrA)

SBAE:

DSM104329_01844(ccr)

TBH:	Tbon_11600(ccrA)

TFLA:

O0235_00785(ccrA)

LIL:	LA_0296(adhP)

LIE:	LIF_A0249(adhP)

LIC:

LIC_10253

LIS:	LIL_10251(adhP1)

LBJ:

LBJ_2607

LBL:

LBL_0505

LBI:	LEPBI_I3117

LBF:

LBF_3008

LST:

LSS_00520

LAJ:	A0128_16875

LMAY:

DPV73_07740(ccrA)

LKM:	EFP84_08590(ccrA)

LWL:	FHG68_03355(ccrA)

LKB:	LPTSP3_g32730(adhP_3)

LNO:	MAL08_01270(ccrA)

LKR:	FH602_04485(ccrA)

TPX:	Turpa_4007

COY:	HF329_03875(ccrA)

» show all

Reference

1 [PMID:17548827]

Authors

Erb TJ, Berg IA, Brecht V, Muller M, Fuchs G, Alber BE

Title

Synthesis of C5-dicarboxylic acids from C2-units involving crotonyl-CoA carboxylase/reductase: the ethylmalonyl-CoA pathway.

Journal

Proc Natl Acad Sci U S A 104:10631-6 (2007)
DOI:10.1073/pnas.0702791104

Sequence

[rsp:RSP_0960]

Reference

2 [PMID:19458256]

Authors

Erb TJ, Brecht V, Fuchs G, Muller M, Alber BE

Title

Carboxylation mechanism and stereochemistry of crotonyl-CoA carboxylase/reductase, a carboxylating enoyl-thioester reductase.

Journal

Proc Natl Acad Sci U S A 106:8871-6 (2009)
DOI:10.1073/pnas.0903939106

Other DBs

ExplorEnz - The Enzyme Database:

1.3.1.85

IUBMB Enzyme Nomenclature:

1.3.1.85

ExPASy - ENZYME nomenclature database:

1.3.1.85

BRENDA, the Enzyme Database:

1.3.1.85

DBGET integrated database retrieval system