KEGG ENZYME: 1.3.7.12

ENZYME: 1.3.7.12

Entry

EC 1.3.7.12                 Enzyme

Name

red chlorophyll catabolite reductase;
RCCR;
RCC reductase;
red Chl catabolite reductase

Class

Oxidoreductases;
Acting on the CH-CH group of donors;
With an iron-sulfur protein as acceptor

Sysname

primary fluorescent chlorophyll catabolite:ferredoxin oxidoreductase

Reaction(IUBMB)

primary fluorescent chlorophyll catabolite + 2 oxidized ferredoxin [iron-sulfur] cluster = red chlorophyll catabolite + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ [RN:R09032]

Reaction(KEGG)

R09032

Substrate

primary fluorescent chlorophyll catabolite [CPD:C18098];
oxidized ferredoxin [iron-sulfur] cluster [CPD:C00139]

Product

red chlorophyll catabolite [CPD:C18022];
reduced ferredoxin [iron-sulfur] cluster [CPD:C00138];
H+ [CPD:C00080]

Comment

The enzyme participates in chlorophyll degradation, which occurs during leaf senescence and fruit ripening in higher plants. The reaction requires reduced ferredoxin, which is generated from NADPH produced either through the pentose-phosphate pathway or by the action of photosystem I [1,2]. This reaction takes place while red chlorophyll catabolite is still bound to EC 1.14.15.17, pheophorbide a oxygenase [3]. Depending on the plant species used as the source of enzyme, one of two possible C-1 epimers of primary fluorescent chlorophyll catabolite (pFCC), pFCC-1 or pFCC-2, is normally formed, with all genera or species within a family producing the same isomer [3,4]. After modification and export, pFCCs are eventually imported into the vacuole, where the acidic environment causes their non-enzymic conversion into colourless breakdown products called non-fluorescent chlorophyll catabolites (NCCs) [2].

History

EC 1.3.7.12 created 2007 as EC 1.3.1.80, transferred 2016 to EC 1.3.7.12

Pathway

ec00860

Porphyrin metabolism

ec01110

Biosynthesis of secondary metabolites

Orthology

K13545

red chlorophyll catabolite reductase

Genes

ATH:	AT4G37000(ACD2)

ALY:

9303041

CRB:

111828541

CSAT:

104716415 104721055 104729460

EUS:	EUTSA_v10025731mg EUTSA_v10027845mg EUTSA_v10027846mg

BRP:

103862886

BNA:	106426866 106438613 111202026

BOE:

106325027

RSZ:

108819142

THJ:

104807582

CPAP:

110814300

CIT:	102622999 102623285

CIC:	CICLE_v10026041mg CICLE_v10026248mg

PVY:	116112407 116116769

MINC:

123193402

TCC:

18611120

GRA:

105803912

GHI:	107897716 107913254

GAB:

108461228

DZI:	111292562 111299984

EGR:

104418575

GMX:	100777640 100780934

GSJ:	114383322 114403224

PVU:	PHAVU_008G280200g PHAVU_008G280300g

VRA:

106763057

VAR:

108337053

VUN:

114195690

CCAJ:

109806983

APRC:

113847072

MTR:

11442328

CAM:

101488374

PSAT:

127117568

LJA:	Lj2g3v3246900.1(Lj2g3v3246900.1)

ADU:

107496225

AIP:

107608673

AHF:	112701797 112764981

LANG:

109356885

FVE:

101301239

RCN:

112185943

PPER:

18769442

PMUM:

103339882

PAVI:

110765868

PDUL:

117634688

MDM:

103401375

PXB:

103964494

ZJU:

107424775

MNT:	21384210 21394345 21394346

CSV:	101202861 101223133

CMO:	103497431 103497432

BHJ:	120089204 120089221

MCHA:

111017870 111017957

CMAX:

111499492 111499662 111499663

CMOS:

111451176 111451765

CPEP:

111785012 111785014 111796165 111796166

RCU:

8262890

JCU:

105639704

HBR:

110646846

MESC:

110629083

POP:	7462586 7483116

PEU:	105138976 105138977 105138985

PALZ:

118047897 118047898

JRE:	108983930 108985767

CILL:

122276873 122296360 122305602

QSU:	111998865 111998868

QLO:	115950885 115950924

TWL:	120011254 120013867 120014953

VVI:

100252439

VRI:

117919356

SLY:

778267

SPEN:

107014711

SOT:

102591737

SSTN:

125859094 125859986

CANN:

107868112

NTA:	107810967 107825518

NSY:

104239946

NTO:

104108838

NAU:

109211050

INI:

109177802

ITR:

116000427

SIND:

105177623 105177624

OEU:

111410341

EGT:

105972914

SSPL:

121741847

APAN:

127241548

HAN:

110909121

ECAD:

122603307

LSV:

111876719

CCAV:

112518578

DCR:

108192987

CSIN:

114260490

BVG:

104883759

SOE:

110777086

CQI:	110682303 110718122

NNU:

104608870

MING:

122069185

TSS:

122651858

PSOM:

113346713 113356881

OSA:	107277220 4348519

DOSA:

Os10t0389100-00(Os10g0389100) Os10t0389200-01(Os10g0389200) Os10t0389300-01(Os10g0389300)

OBR:

102703616

OGL:	127785751 127785752

BDI:

100843855

ATS:	109745881 109767332

TDC:	119314655 119326411 119331775 119336169 119357510

TAES:

123129900 123133085 123138571 123142922 123153767 123156544 123164229

TUA:	125517190 125517199 125542622

LPER:

127304710 127317400

SBI:	8062831 8068743 8079617

ZMA:

100272914

SITA:

101780665

SVS:

117840638

PVIR:

120651187 120691151

PHAI:

112873608 112878352

PDA:

103701815

EGU:	105041580 105048691

MUS:

103999342

ZOF:	121971621 121973772 121976029 121977835 121978528

DCT:

110101222

PEQ:

110027504

AOF:

109827526

NCOL:

116252082 116253048

ATR:

18421357

SMO:	SELMODRAFT_186296 SELMODRAFT_269565

PPP:

112292462

» show all

Reference

1 [PMID:12223835]

Authors

Rodoni S, Muhlecker W, Anderl M, Krautler B, Moser D, Thomas H, Matile P, Hortensteiner S.

Title

Chlorophyll Breakdown in Senescent Chloroplasts (Cleavage of Pheophorbide a in Two Enzymic Steps).

Journal

Plant Physiol 115:669-676 (1997)
DOI:10.1104/pp.115.2.669

Reference

2 [PMID:10743659]

Authors

Wuthrich KL, Bovet L, Hunziker PE, Donnison IS, Hortensteiner S

Title

Molecular cloning, functional expression and characterisation of RCC reductase involved in chlorophyll catabolism.

Journal

Plant J 21:189-98 (2000)
DOI:10.1046/j.1365-313x.2000.00667.x

Sequence

[ag:CAB77705]

Reference

3 [PMID:17237353]

Authors

Pruzinska A, Anders I, Aubry S, Schenk N, Tapernoux-Luthi E, Muller T, Krautler B, Hortensteiner S.

Title

In vivo participation of red chlorophyll catabolite reductase in chlorophyll breakdown.

Journal

Plant Cell 19:369-87 (2007)
DOI:10.1105/tpc.106.044404

Sequence

[ath:AT4G37000]

Reference

4 [PMID:16669755]

Authors

Hortensteiner S.

Title

Chlorophyll degradation during senescence.

Journal

Annu Rev Plant Biol 57:55-77 (2006)
DOI:10.1146/annurev.arplant.57.032905.105212

Reference

5 [PMID:12223836]

Authors

Rodoni S, Vicentini F, Schellenberg M, Matile P, Hortensteiner S.

Title

Partial Purification and Characterization of Red Chlorophyll Catabolite Reductase, a Stroma Protein Involved in Chlorophyll Breakdown.

Journal

Plant Physiol 115:677-682 (1997)
DOI:10.1104/pp.115.2.677

Other DBs

ExplorEnz - The Enzyme Database:

1.3.7.12

IUBMB Enzyme Nomenclature:

1.3.7.12

ExPASy - ENZYME nomenclature database:

1.3.7.12

BRENDA, the Enzyme Database:

1.3.7.12

DBGET integrated database retrieval system