KEGG   ENZYME: 1.3.98.1
Entry
EC 1.3.98.1                 Enzyme                                 

Name
dihydroorotate dehydrogenase (fumarate);
DHOdehase (ambiguous);
dihydroorotate dehydrogenase (ambiguous);
dihydoorotic acid dehydrogenase (ambiguous);
DHOD (ambiguous);
DHODase (ambiguous);
dihydroorotate oxidase;
pyr4 (gene name)
Class
Oxidoreductases;
Acting on the CH-CH group of donors;
With other, known, physiological acceptors
Sysname
(S)-dihydroorotate:fumarate oxidoreductase
Reaction(IUBMB)
(S)-dihydroorotate + fumarate = orotate + succinate [RN:R01867]
Reaction(KEGG)
R01867
Substrate
(S)-dihydroorotate [CPD:C00337];
fumarate [CPD:C00122]
Product
orotate [CPD:C00295];
succinate [CPD:C00042]
Comment
Binds FMN. The reaction, which takes place in the cytosol, is the only redox reaction in the de novo biosynthesis of pyrimidine nucleotides. Molecular oxygen can replace fumarate in vitro. Other class 1 dihydroorotate dehydrogenases use either NAD+ (EC 1.3.1.14) or NADP+ (EC 1.3.1.15) as electron acceptor. The membrane bound class 2 dihydroorotate dehydrogenase (EC 1.3.5.2) uses quinone as electron acceptor.
History
EC 1.3.98.1 created 1961 as EC 1.3.3.1, transferred 2011 to EC 1.3.98.1
Pathway
ec00240  Pyrimidine metabolism
ec01100  Metabolic pathways
Orthology
K00226  dihydroorotate dehydrogenase (fumarate)
Genes
QSU: 112014477
SCE: YKL216W(URA1)
KLA: KLLA0_C19360g
KMX: KLMA_10832(URA1)
LTH: KLTH0F00308g
ZRO: ZYRO0D00396g
NCS: NCAS_0D03520(NCAS0D03520)
NDI: NDAI_0H00120(NDAI0H00120)
TPF: TPHA_0K00200(TPHA0K00200)
TBL: TBLA_0B10140(TBLA0B10140)
TDL: TDEL_0F05620(TDEL0F05620)
KAF: KAFR_0L00170(KAFR0L00170)
NCR: NCU04318
NTE: NEUTE1DRAFT15063(NEUTE1DRAFT_15063)
MGR: MGG_07263
SSCK: SPSK_01211
MAW: MAC_06929
MAJ: MAA_00067
BFU: BCIN_16g04110(Bcura1)
PTE: PTT_01403
TASA: A1Q1_07191
ABP: AGABI1DRAFT51017(AGABI1DRAFT_51017)
ABV: AGABI2DRAFT214748(AGABI2DRAFT_214748)
SMIN: v1.2.031230.t1(symbB.v1.2.031230.t1)
SPAR: SPRG_07872
LMA: LMJF_16_0530(DHODH)
LIF: LINJ_16_0540(DHODH)
LBZ: LBRM_16_0550(DHODH)
LPAN: LPMP_160530(DHODH)
EAM: EAMY_0442
EAY: EAM_2979
PLU: plu3917
HSO: HS_0799
HSM: HSM_1268
MHT: D648_7350
MHAT: B824_7980
MHAE: F382_11785
MHAM: J450_10470
MHAO: J451_11895
MHAL: N220_03905
MHAQ: WC39_09975
MHAY: VK67_09975
MVI: X808_6700
MVG: X874_6790
BTO: WQG_17660
BTRE: F542_4910
BTRH: F543_5590
BTRA: F544_17520
SVO: SVI_3253(pyrD-2)
SPSW: Sps_04212
SBK: SHEWBE_3881(pyrDA)
MAD: HP15_3391
MCA: MCA0768
METL: U737_16125
MMAI: sS8_1223
THIP: N838_16860
TBN: TBH_C0805
ECOR: SAMEA4412678_1339(pyrDA)
CVI: CV_3551(pydA)
CHAE: CH06BL_34350(pyrDA)
LHK: LHK_00854
AMAH: DLM_0496
TBD: Tbd_2467
DAR: Daro_2839
SULR: B649_05420
DPS: DP0769
DML: Dmul_38520(pyrD2)
DALK: DSCA_43100
ADE: Adeh_0762
MSL: Msil_2988
PZU: PHZ_p0133
RCP: RCAP_rcc01727(pyrD1)
ASZ: ASN_2514
MAG: amb2359
MAGX: XM1_3143
MAGN: WV31_07695
SAU: SA2375
SAV: SAV2589
SAW: SAHV_2573
SAM: MW2509
SAS: SAS2475
SAR: SAR2669
SAC: SACOL2606(pyrD)
SAX: USA300HOU_2583(pyrD)
SAA: SAUSA300_2526(pyrD)
SAE: NWMN_2488(pyrD)
SAD: SAAV_2655(pyrD)
SUE: SAOV_2634
SUJ: SAA6159_02486(pyrD)
SUK: SAA6008_02648(pyrD)
SUC: ECTR2_2443(pyrD)
SUQ: HMPREF0772_10602(pyrD)
SUZ: MS7_2595(pyrD)
SUG: SAPIG2639(pyrD)
SAUA: SAAG_00409
SAUE: RSAU_002430(pyrD)
SAUS: SA40_2343
SAUU: SA957_2427
SAUG: SA268_2505
SAUF: X998_2573
SAB: SAB2464
SAUB: C248_2650
SAUC: CA347_2666(pyrD)
SAUR: SABB_02002
SAUI: AZ30_13560
SAUD: CH52_06060
SAMS: NI36_12960
SER: SERP2144(pyrD)
SEP: SE2132
SEPP: SEB_02136
SEPS: DP17_1072(pyrD)
SHA: SH0471
SHH: ShL2_00375(pyrD)
SSP: SSP0278
SPAS: STP1_1082
SXO: SXYL_00310(pyrD)
SCAP: AYP1020_1752(pyrD)
SARL: SAP2_04040
SSH: NCTC13712_02544(pyrD)
SSIM: SAMEA4384339_2389(pyrD)
GMO: NCTC11323_00512(pyrDA)
GHA: NCTC10459_00606(pyrDA)
LLA: L192589(pydA)
LLK: LLKF_1674(pydA)
LLT: CVCAS_1457(pydA)
LLS: lilo_1474(pydA)
LLX: NCDO2118_1571(pyrDA)
LLJ: LG36_1498(pydA)
LLM: llmg_0952(pyrDA)
LLC: LACR_1644
LLR: llh_4795
LLI: uc509_1504(pyrDA)
LLW: kw2_1506
LGR: LCGT_1508
LGV: LCGL_1530
SPY: SPy_1432(pyrD)
SPZ: M5005_Spy1165(pyrD)
SPYM: M1GAS476_1227(pyrD)
SPYA: A20_1200(pyrD)
SPG: SpyM3_1091(pyrD)
SPS: SPs0774
SPH: MGAS10270_Spy1236(pyrD)
SPI: MGAS10750_Spy1273(pyrD)
SPJ: MGAS2096_Spy1237(pyrD)
SPK: MGAS9429_Spy1214(pyrD)
SPF: SpyM50695(pyrD)
SPB: M28_Spy1159(pyrD)
STG: MGAS15252_1104(pyrD)
STX: MGAS1882_1099(pyrD)
SOZ: Spy49_1144(pyrD)
STZ: SPYALAB49_001164(pyrD)
SPYH: L897_05790
SPN: SP_0764(pyrDA)
SPD: SPD_0665(pyrDa)
SPR: spr0672(pyrDA)
SPW: SPCG_0713
SJJ: SPJ_0701
SNV: SPNINV200_06750(pyrD)
SPX: SPG_0695
SNT: SPT_0777
SND: MYY_0796
SPNN: T308_03565
SNE: SPN23F06860(pyrD)
SPV: SPH_0863
SPP: SPP_0773
SNI: INV104_06350(pyrD)
SPNG: HMPREF1038_00774(pyrDA)
SNP: SPAP_0737
SNX: SPNOXC06910(pyrD)
SNU: SPNA45_01088(pyrD)
SPNE: SPN034156_17400(pyrD)
SPNU: SPN034183_06920(pyrD)
SPNM: SPN994038_06810(pyrD)
SPNO: SPN994039_06820(pyrD)
SAG: SAG0507(pyrDA)
SAN: gbs0553
SAK: SAK_0657(pyrD)
SGC: A964_0538(pyrD)
SAGS: SaSA20_0492(pyrD)
SAGL: GBS222_0484(pyrD)
SAGM: BSA_5950
SAGI: MSA_6100
SAGR: SAIL_6240
SAGP: V193_02825
SAGC: DN94_02825
SAGE: EN72_02920
SAGG: EN73_02995
SAGN: W903_0684
SMU: SMU_595(pyrD)
SMC: SmuNN2025_1383(pyrD)
SMJ: SMULJ23_1390(pyrD)
SMUA: SMUFR_0516(pyrD)
STC: str1207(pyrDa)
STL: stu1207(pyrDa)
STE: STER_1173
STN: STND_1143
STU: STH8232_1412(pyrD2)
STW: Y1U_C1109
STHE: T303_06890
SSA: SSA_0373(pyrDA)
SSB: SSUBM407_0226(pyrD)
SSI: SSU0235(pyrD)
SSS: SSUSC84_0224(pyrD)
SUP: YYK_01095
SSUY: YB51_1255
SSUT: TL13_0279(pyrD)
SSUI: T15_0241(pyrD)
SGO: SGO_0277(pyrA)
SEZ: Sez_0633
SEQ: SZO_13640
SEZO: SeseC_00765(pyrD)
SEQU: Q426_06160
SEU: SEQ_0655
SUB: SUB1264(pyrD)
SDS: SDEG_1471(pyrD)
SDA: GGS_1341(pyrD)
SDC: SDSE_1579(pyrD)
SDQ: SDSE167_1579(pyrD)
SGA: GALLO_0639(pyrD)
SGG: SGGBAA2069_c05820(pyrD)
SGT: SGGB_0614(pyrD1)
SMB: smi_0815(pyrD2)
SOR: SOR_0779(pyrD)
STK: STP_1098
STB: SGPB_0508(pyrD.1)
SCF: Spaf_1800(pyrD3)
SSR: SALIVB_1200(pyrD)
STF: Ssal_01281(pyrD)
STJ: SALIVA_0882(pyrD)
SSAH: HSISS4_00782(pyrDa)
SMN: SMA_0601(pyrD)
SIF: Sinf_0520(pyrD)
SIE: SCIM_0207(pyrD)
SIB: SIR_0264(pyrDa)
SIU: SII_0247(pyrDa)
SANG: SAIN_1602(pyrDa)
SANC: SANR_1834(pyrDa)
SANS: DK43_01485
SCG: SCI_0264(pyrDa)
SCON: SCRE_0244(pyrDa)
SCOS: SCR2_0244(pyrDa)
SIK: K710_0597
SLU: KE3_0599
STRN: SNAG_0804(pyrDA)
STRG: SRT_15260(pyrD)
SVB: NCTC12167_01130(pyrDa)
SMEN: SAMEA4412692_1637(pyrDA)
SFER: NCTC12278_00551(pyrD)
SCAI: NCTC12191_01271(pyrD)
LJO: LJ_1281
LJF: FI9785_918(pyrD)
LJH: LJP_0899
LAC: LBA1384(pyrD)
LAD: LA14_1382
LAF: SD55_1367(pyrD)
LDB: Ldb1530(pyrD2) Ldb2114(pyrD1)
LDE: LDBND_1465(pyrD2)
LGA: LGAS_1092
LHE: lhv_2937
LHL: LBHH_0706
LHV: lhe_1366
LHH: LBH_1217
LHD: HUO_04845
LCR: LCRIS_01374(pyrD)
LAM: LA2_07985
LKE: WANG_0334
LAE: LBAT_0593
LPW: LpgJCM5343_08490(pyrD)
LCA: LSEI_1732
LCS: LCBD_1929
LCE: LC2W_1908
LCW: BN194_19150(pyrDA)
LPAP: LBPC_1652
LCB: LCABL_19520(pyrDA)
LRH: LGG_01789(pyrD)
LRG: LRHM_1725
LRL: LC705_01772(pyrD)
LRA: LRHK_1764(ura1)
LFE: LAF_1201
LFR: LC40_0780
LFF: LBFF_1312
LSN: LSA_05870(pyrDA)
LBH: Lbuc_0385
LBN: LBUCD034_0423(pyrD)
LHIL: G8J22_00338(pyrDA)
LSL: LSL_0194(pyrD)
LSI: HN6_00184(pyrD)
LSJ: LSJ_0237(pyrD)
LRM: LRC_09270
LSA: LCA_0446
OOE: OEOE_0264
LME: LEUM_1279
LMM: MI1_05585
LMK: LMES_1061
LKI: LKI_09935
LGS: LEGAS_1084(pyrDA)
WCE: WS08_0824
WCT: WS74_0890
WSO: WSWS_00901(pyrD)
EFA: EF0285(pyrD-1)
EFL: EF62_0674(pyrD-1)
EFI: OG1RF_10228(pyrDA)
EFS: EFS1_0226(pyrD-1)
EFN: DENG_00270(pyrD)
EFQ: DR75_2333(ura1)
EFC: EFAU004_00893(pyrDA)
EFAU: EFAU085_01453(pyrDA)
EFU: HMPREF0351_11431(pyrD2)
EFM: M7W_1926
EHR: EHR_11760
ECAS: ECBG_02253
EMU: EMQU_1428(pyrDA)
EDU: LIU_08370
ESG: EsVE80_11910(pyrDA)
THL: TEH_14370(pyrD)
CDF: CD630_20770(pyrD1)
PDC: CDIF630_02300(preA1)
CDC: CD196_1941(pyrD)
CDL: CDR20291_1984(pyrD)
PDF: CD630DERM_20770(pyrD1)
ATE: Athe_2085
MMC: Mmcs_4864
MKM: Mkms_4953
MJL: Mjls_5232
MMI: MMAR_3409
MCOO: MCOO_35610
MSEO: MSEO_01210
MVA: Mvan_4110
MHEV: MHEL_40650
SBH: SBI_02815
PAC: PPA0161
PAW: PAZ_c01720(pyrD1)
PACC: PAC1_00855
PACH: PAGK_0189
CACN: RN83_01250
CGRN: 4412665_00536(pyrD)
PAUS: NCTC13651_00002(preA_1) NCTC13651_01709(preA_2)
ACIJ: JS278_02796(preA)
TLA: TLA_TLA_01037(preA)
FAL: FRAAL1902
BLO: BL1213
SYN: sll0744
SYY: SYNGTS_2808(sll0744)
SYT: SYNGTI_2807(sll0744)
SYS: SYNPCCN_2806(sll0744)
SYQ: SYNPCCP_2806(sll0744)
SYC: syc1720_c
AMR: AM1_D0141(pyrD)
MAR: MAE_38150
MPK: VL20_5511
CYT: cce_3252
ARP: NIES39_L04100(pyrD)
PAGH: NIES204_36800(pyrD_2)
ANA: alr1912
AVA: Ava_3045
ANB: ANA_C11950(pyrD)
DOU: BMF77_00528(preA)
CTHE: Chro_4406
STAN: STA3757_05380(pyrD_1)
RCA: Rcas_2064
CAU: Caur_2081
CAG: Cagg_2635
ATM: ANT_28310
TBC: A0O31_00009(pyrD)
OTE: Oter_0606
OBG: Verru16b_00598(preA_1) Verru16b_01854(preA_2)
CAA: Caka_1151
MIN: Minf_1387(pyrD) Minf_2385(pyrD)
MEAP: MTHMO_0210
RBA: RB4738 RB4752(pyrD)
RUL: UC8_16580(pyrDB) UC8_27550
AHEL: Q31a_47690(pyrDB_1) Q31a_48380(pyrDB_2)
TTF: THTE_4057
AMUC: Pan181_30330(pyrD_1)
PND: Pla175_23340(pyrD)
PLH: VT85_05270(preA)
FMR: Fuma_01332(preA)
MRI: Mal4_54110(preA)
ULI: ETAA1_62400(preA)
AGV: OJF2_24280(preA)
PCOR: KS4_01440(pyrDB_1)
VBL: L21SP4_00337(pyrD_1)
ABAS: ACPOL_5878
SUS: Acid_6995
ABAC: LuPra_04112(preA_2)
LBA: Lebu_2234
BTH: BT_1333
BTHO: Btheta7330_01888(preA)
BFR: BF2950
BXY: BXY_43000
BOA: Bovatus_01352(preA)
BCEL: BcellWH2_05077(preA)
BVU: BVU_3869
PGI: PG_2055
PGN: PGN_1999
PCRE: NCTC12858_00853(pyrD_1)
PCAG: NCTC12856_00153(preA)
PMUC: ING2E5A_0046(URA1) ING2E5A_0807(pyrD1) ING2E5A_2689(pyrD5)
PDI: BDI_0543
TFO: BFO_0242
AFD: Alfi_1991
BLQ: L21SP5_00505(pyrD_2) L21SP5_00918(preA) L21SP5_01040(pyrDA_1) L21SP5_02712(pyrDA_2)
SGN: SGRA_p0002(pyrD)
FBU: UJ101_01334(pyrDI)
EAO: BD94_0307
ELB: VO54_02286(pyrDA)
BPI: BPLAN_127(pyrD)
BMM: MADAR_234(pyrD)
BCP: BLBCPU_481(pyrD)
BBG: BGIGA_124(pyrD)
BLP: BPAA_124(pyrD)
BLU: K645_2431
CLI: Clim_2445
PVI: Cvib_1724
PLT: Plut_2084
PAA: Paes_2219
PROC: Ptc2401_02228(pyrD_2)
CTS: Ctha_0742
IAL: IALB_2950(pyrD)
MRO: MROS_2662
TLE: Tlet_1878
TME: Tmel_0121
CABY: Cabys_261
DTU: Dtur_0444
NDE: NIDE1091(pyrD)
NMV: NITMOv2_0967(pyrD)
NIO: NITINOP_2763(pyrD)
NJA: NSJP_2098(pyrDA)
STO: STK_14770(pyrD)
SSO: SSO0610(pyrD)
SOL: Ssol_1674
SSOA: SULA_1702
SSOL: SULB_1703
SSOF: SULC_1701
SAI: Saci_1592(pyrD)
SID: M164_1519
SII: LD85_1729
SIH: SiH_1488
SIR: SiRe_1397
SIC: SiL_1400
MSE: Msed_1960
MCN: Mcup_0324
AHO: Ahos_1133
STEP: IC006_1331
PAI: PAE3263
PIS: Pisl_0507
PCL: Pcal_1778
PAS: Pars_1692
PYR: P186_1337
POG: Pogu_0443
TNE: Tneu_1590
TTN: TTX_0796(pyrD)
TUZ: TUZN_0505
VDI: Vdis_1107
VMO: VMUT_1972
 » show all
Reference
1  [PMID:9405053]
  Authors
Bjornberg O, Rowland P, Larsen S, Jensen KF
  Title
Active site of dihydroorotate dehydrogenase A from Lactococcus lactis investigated by chemical modification and mutagenesis.
  Journal
Biochemistry 36:16197-205 (1997)
DOI:10.1021/bi971628y
  Sequence
[llm:llmg_0952]
Reference
2  [PMID:9655329]
  Authors
Rowland P, Bjornberg O, Nielsen FS, Jensen KF, Larsen S
  Title
The crystal structure of Lactococcus lactis dihydroorotate dehydrogenase A complexed with the enzyme reaction product throws light on its enzymatic function.
  Journal
Protein Sci 7:1269-79 (1998)
DOI:10.1002/pro.5560070601
  Sequence
[llm:llmg_0952]
Reference
3  [PMID:12732650]
  Authors
Norager S, Arent S, Bjornberg O, Ottosen M, Lo Leggio L, Jensen KF, Larsen S
  Title
Lactococcus lactis dihydroorotate dehydrogenase A mutants reveal important facets of the enzymatic function.
  Journal
J Biol Chem 278:28812-22 (2003)
DOI:10.1074/jbc.M303767200
  Sequence
[llm:llmg_0952]
Reference
4  [PMID:17617217]
  Authors
Zameitat E, Pierik AJ, Zocher K, Loffler M
  Title
Dihydroorotate dehydrogenase from Saccharomyces cerevisiae: spectroscopic investigations with the recombinant enzyme throw light on catalytic properties and metabolism of fumarate analogues.
  Journal
FEMS Yeast Res 7:897-904 (2007)
DOI:10.1111/j.1567-1364.2007.00275.x
  Sequence
[sce:YKL216W]
Reference
5  [PMID:18808149]
  Authors
Inaoka DK, Sakamoto K, Shimizu H, Shiba T, Kurisu G, Nara T, Aoki T, Kita K, Harada S
  Title
Structures of Trypanosoma cruzi dihydroorotate dehydrogenase complexed with substrates and products: atomic resolution insights into mechanisms of dihydroorotate oxidation and fumarate reduction.
  Journal
Biochemistry 47:10881-91 (2008)
DOI:10.1021/bi800413r
  Sequence
[tcr:508375.50]
Reference
6  [PMID:19932077]
  Authors
Cheleski J, Wiggers HJ, Citadini AP, da Costa Filho AJ, Nonato MC, Montanari CA
  Title
Kinetic mechanism and catalysis of Trypanosoma cruzi dihydroorotate dehydrogenase enzyme evaluated by isothermal titration calorimetry.
  Journal
Anal Biochem 399:13-22 (2010)
DOI:10.1016/j.ab.2009.11.018
Other DBs
ExplorEnz - The Enzyme Database: 1.3.98.1
IUBMB Enzyme Nomenclature: 1.3.98.1
ExPASy - ENZYME nomenclature database: 1.3.98.1
BRENDA, the Enzyme Database: 1.3.98.1
CAS: 9029-03-2

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