KEGG   ENZYME: 1.3.99.27
Entry
EC 1.3.99.27                Enzyme                                 
Name
1-hydroxycarotenoid 3,4-desaturase;
CrtD;
hydroxyneurosporene desaturase;
carotenoid 3,4-dehydrogenase;
1-hydroxy-carotenoid 3,4-dehydrogenase
Class
Oxidoreductases;
Acting on the CH-CH group of donors;
With unknown physiological acceptors
Sysname
1-hydroxy-1,2-dihydrolycopene:acceptor oxidoreductase
Reaction(IUBMB)
1-hydroxy-1,2-dihydrolycopene + acceptor = 1-hydroxy-3,4-didehydro-1,2-dihydrolycopene + reduced acceptor [RN:R07520]
Reaction(KEGG)
R07520;
(other) R07523 R07534
Substrate
1-hydroxy-1,2-dihydrolycopene [CPD:C19795];
acceptor [CPD:C00028]
Product
1-hydroxy-3,4-didehydro-1,2-dihydrolycopene [CPD:C15874];
reduced acceptor [CPD:C00030]
Comment
The enzymes from Rubrivivax gelatinosus and Rhodobacter sphaeroides prefer the acyclic carotenoids (e.g. 1-hydroxy-1,2-dihydroneurosporene, 1-hydroxy-1,2-dihydrolycopene) as substrates. The conversion rate for the 3,4-desaturation of the monocyclic 1'-hydroxy-1',2'-dihydro-gamma-carotene is lower [2,3]. The enzyme from the marine bacterium strain P99-3 shows high activity with the monocyclic carotenoid 1'-hydroxy-1',2'-dihydro-gamma-carotene [1]. The enzyme from Rhodobacter sphaeroides utilizes molecular oxygen as the electron acceptor in vitro [3]. However, oxygen is unlikely to be the natural electron acceptor under anaerobic conditions.
History
EC 1.3.99.27 created 2011
Pathway
ec00906  Carotenoid biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K09845  1-hydroxycarotenoid 3,4-desaturase
Genes
ALVAlvin_2566
ATEPAtep_25190
TVIThivi_2015
TMBThimo_3009
TSYTHSYN_31070
TTPE6P07_01720(crtI)
THIPN838_26740(crtI)
CJAPGWK36_12660(crtI)
THIMKFB96_13175(crtI) KFB96_13180(crtI)
TBOGLT988_08925(crtI)
HHAHhal_1614
HHCM911_10370
EBSECTOBSL9_3050
PDQCL55_00013470
RACRA876_06050
LIML103DPR2_00983(crtD)
LIHL63ED372_00638(crtD)
HYRBSY239_1370(crtI)
METPC1M51_03595
RGERGE_33590(crtD)
RBNRBXJA2T_09492
RDPRD2015_1033
METRBSY238_2435(crtI)
BEBAEM42_07445
MSDMYSTI_00907
MYMA176_006084
MMASMYMAC_000921
MBDMEBOL_004336
CFUSCYFUS_001183
SCLsce0585(crtD)
SCUSCE1572_04055
CCROCMC5_014490
MRMA7982_08935
BSEDDN745_03165
PCAYFRD00_10895(crtI)
RPODE0E05_11770(crtI)
AGCBSY240_3672(crtI)
ABAWD5400_04850(crtI)
PDESFE840_002305(crtI)
BRABRADO1614(crtD)
BBTBBta_6441(crtD)
BRSS23_19140(crtD)
AOLS58_59220
BROBRAD285_5786(crtD)
BRQCIT40_23960
BGZXH91_02625
BBETF8237_24655(crtI)
RPARPA1518(crtD)
RPBRPB_4005
RPCRPC_1289
RPDRPD_3760
RPERPE_1323
RPTRpal_1706
RPXRpdx1_4002
BOSBSY19_4059(crtI)
MEAMex_1p2923
MDIMETDI3492
MEXMext_2726
MCHMchl_2953
MPOMpop_2848
MZAB2G69_21170
MRDMrad2831_2822
METM446_3693
MORMOC_3187
METAY590_13120
MAQUMaq22A_1p32525
MPHYMCBMB27_02263
MEEDA075_03940
METDC0214_15285
METXA3862_17680
METSDK389_31455
METIDK427_17915
MMESMMSR116_13595(crtI)
MTEADK419_04895
MINDmvi_08940
MOGMMB17_23905(crtI)
MSLMsil_2048
BBARRHAL1_03018(crtD)
RVARvan_0207
BVRBVIR_3174
BLAGBLTE_15830
MROSEHO51_17015(crtI)
AUBLXB15_06395(crtI)
BSBBresu_3048
BRLBZG35_15470
RUTFIU92_10175(crtD)
JANJann_0183(crtD)
RDERD1_0115(crtD)
RLIRLO149_p940120(crtD)
RPONG3256_18640(crtI)
DSHDshi_3513(crtD)
REDroselon_01074
SUAMBOO69_19430
TOMBWR18_20220
RMMROSMUCSMR3_00909(crtD)
ROKRAK1035_3778
RIDRIdsm_01751(crtP)
ROMEI983_05420(crtI)
ROHFIU89_14255(crtD)
THAACFI11_23530
MARUFIU81_13880(crtD)
ROTFIV09_18050(crtD)
MALUKU6B_16390(crtD)
RSPRSP_0266(crtD)
RSHRsph17029_1910
RSQRsph17025_2040
RSKRSKD131_1593
CAZTLV780_09095(crtI)
RCPRCAP_rcc00683(crtD)
RBLB6K69_00170
PTPRCA23_c29580(crtD)
RSUNHU_04283
RHMB5V46_17505
RHCRGUI_1965
LVSLOKVESSMR4R_03675(crtD)
RBGBG454_03015
GEHHYN69_05505
TAWEI545_18925(crtI)
POZI0K15_00030(crtI)
FAQG5B39_11900(crtI)
HBCAEM38_14635
NGFFRF71_09130(crtI)
NOGGKE62_08420(crtI)
SPHITS85_22890
SSANNX02_18465
SCHYGVO57_11645(crtI)
CIJWG74_05030
BLASBSY18_2718(crtI)
BFWB5J99_04540
PALGHFP57_06965(crtI)
PARJJ4G78_07550(crtI)
SANDH3309_05735
POQKZX46_18720(crtI)
ALHG6N82_11415(crtI)
ELQGa0102493_111841
ERKCD351_13855
ERFFIU90_13815(crtD)
PNSA9D12_01275
PORLBG023_112667
PHZCHX26_14485
POTE2E27_07350(crtI)
AMVACMV_18830(crtD)
NCBC0V82_18185
SKTIGS68_17135(crtI)
SMUCJL100_008900(crtI)
SROEJL101_015315(crtI)
RRURru_A2984
RRFF11_15285
RCERC1_2090(crtD)
RPMRSPPHO_02323(crtD)
ELIOKO353_03095(crtI)
DGEDgeo_2306
DDRDeide_2p01180
DMRDeima_3265
DPTDeipr_1739
DGODGo_CA2871(crtD)
DPDDeipe_2602
DSWQR90_14400
DPUSU48_01075
DEZDKM44_13750
DWUDVJ83_08920
DFCDFI_00330
DEINDAAJ005_15600
DGADEGR_28030
MRBMrub_0386
MREK649_01555
MSVMesil_1492
MTAIMtai_v1c24730
 » show all
Reference
1  [PMID:15251462]
  Authors
Teramoto M, Rahlert N, Misawa N, Sandmann G.
  Title
1-Hydroxy monocyclic carotenoid 3,4-dehydrogenase from a marine bacterium that produces myxol.
  Journal
FEBS Lett 570:184-8 (2004)
DOI:10.1016/j.febslet.2004.05.085
Reference
2  [PMID:10880364]
  Authors
Steiger S, Astier C, Sandmann G
  Title
Substrate specificity of the expressed carotenoid 3,4-desaturase from Rubrivivax gelatinosus reveals the detailed reaction sequence to spheroidene and spirilloxanthin.
  Journal
Biochem J 349:635-40 (2000)
DOI:10.1042/bj3490635
  Sequence
[rge:RGE_33590]
Reference
3  [PMID:9393712]
  Authors
Albrecht M, Ruther A, Sandmann G
  Title
Purification and biochemical characterization of a hydroxyneurosporene desaturase involved in the biosynthetic pathway of the carotenoid spheroidene in Rhodobacter sphaeroides.
  Journal
J Bacteriol 179:7462-7 (1997)
DOI:10.1128/JB.179.23.7462-7467.1997
  Sequence
[rsp:RSP_0266]
Other DBs
ExplorEnz - The Enzyme Database: 1.3.99.27
IUBMB Enzyme Nomenclature: 1.3.99.27
ExPASy - ENZYME nomenclature database: 1.3.99.27
BRENDA, the Enzyme Database: 1.3.99.27

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