KEGG   ENZYME: 1.3.99.27Help
Entry
EC 1.3.99.27                Enzyme                                 

Name
1-hydroxycarotenoid 3,4-desaturase;
CrtD;
hydroxyneurosporene desaturase;
carotenoid 3,4-dehydrogenase;
1-hydroxy-carotenoid 3,4-dehydrogenase
Class
Oxidoreductases;
Acting on the CH-CH group of donors;
With unknown physiological acceptors
BRITE hierarchy
Sysname
1-hydroxy-1,2-dihydrolycopene:acceptor oxidoreductase
Reaction(IUBMB)
1-hydroxy-1,2-dihydrolycopene + acceptor = 1-hydroxy-3,4-didehydro-1,2-dihydrolycopene + reduced acceptor [RN:R07520]
Reaction(KEGG)
R07520;
(other) R07523 R07534
Show
Substrate
1-hydroxy-1,2-dihydrolycopene [CPD:C19795];
acceptor [CPD:C00028]
Product
1-hydroxy-3,4-didehydro-1,2-dihydrolycopene [CPD:C15874];
reduced acceptor [CPD:C00030]
Comment
The enzymes from Rubrivivax gelatinosus and Rhodobacter sphaeroides prefer the acyclic carotenoids (e.g. 1-hydroxy-1,2-dihydroneurosporene, 1-hydroxy-1,2-dihydrolycopene) as substrates. The conversion rate for the 3,4-desaturation of the monocyclic 1'-hydroxy-1',2'-dihydro-gamma-carotene is lower [2,3]. The enzyme from the marine bacterium strain P99-3 shows high activity with the monocyclic carotenoid 1'-hydroxy-1',2'-dihydro-gamma-carotene [1]. The enzyme from Rhodobacter sphaeroides utilizes molecular oxygen as the electron acceptor in vitro [3]. However, oxygen is unlikely to be the natural electron acceptor under anaerobic conditions.
History
EC 1.3.99.27 created 2011
Pathway
ec00906  Carotenoid biosynthesis
ec01100  Metabolic pathways
Orthology
K09845  1-hydroxycarotenoid 3,4-desaturase
Genes
ALV: Alvin_2566
TVI: Thivi_2015
TMB: Thimo_3009
MPUR: MARPU_13290
TSY: THSYN_31065 THSYN_31070
HHA: Hhal_1614
HHC: M911_10370
EBS: ECTOBSL9_3050
PDQ: CL55_00013470
RAC: RA876_06050
LIM: L103DPR2_00983(crtD)
LIH: L63ED372_00638(crtD)
HYR: BSY239_1370(crtI)
RGE: RGE_33590(crtD)
METR: BSY238_2435(crtI)
SCL: sce0585(crtD)
AGC: BSY240_3672(crtI)
BRA: BRADO1614(crtD)
BBT: BBta_6441(crtD)
BRS: S23_19140(crtD)
AOL: S58_59220
BRO: BRAD285_5786(crtD)
RPA: RPA1518(crtD)
RPB: RPB_4005
RPC: RPC_1289
RPD: RPD_3760
RPE: RPE_1323
RPT: Rpal_1706
BOS: BSY19_4059(crtI)
MEX: Mext_2726
MDI: METDI3492
MCH: Mchl_2953
MET: M446_3693
MPO: Mpop_2848
MOR: MOC_3187
META: Y590_13120
MSL: Msil_2048
RVA: Rvan_0207
BVR: BVIR_3174
RSP: RSP_0266(crtD)
RCP: RCAP_rcc00683(crtD)
JAN: Jann_0183(crtD)
RDE: RD1_0115(crtD)
RLI: RLO149_p940120(crtD)
DSH: Dshi_3513(crtD)
PTP: RCA23_c29580(crtD)
RSU: NHU_04283
RHC: RGUI_1965
RMM: ROSMUCSMR3_00909(crtD)
LVS: LOKVESSMR4R_03675(crtD)
SPHI: TS85_22890
SSAN: NX02_18465
BLAS: BSY18_2718(crtI)
AMV: ACMV_18830(crtD)
RRU: Rru_A2984
RRF: F11_15285
RCE: RC1_2090(crtD)
RPM: RSPPHO_02323(crtD)
 » show all
Taxonomy
Reference
1  [PMID:15251462]
  Authors
Teramoto M, Rahlert N, Misawa N, Sandmann G.
  Title
1-Hydroxy monocyclic carotenoid 3,4-dehydrogenase from a marine bacterium that produces myxol.
  Journal
FEBS Lett 570:184-8 (2004)
DOI:10.1016/j.febslet.2004.05.085
Reference
2  [PMID:10880364]
  Authors
Steiger S, Astier C, Sandmann G
  Title
Substrate specificity of the expressed carotenoid 3,4-desaturase from Rubrivivax  gelatinosus reveals the detailed reaction sequence to spheroidene and spirilloxanthin.
  Journal
Biochem J 349:635-40 (2000)
  Sequence
[rge:RGE_33590]
Reference
3  [PMID:9393712]
  Authors
Albrecht M, Ruther A, Sandmann G.
  Title
Purification and biochemical characterization of a hydroxyneurosporene desaturase involved in the biosynthetic pathway of the carotenoid spheroidene in Rhodobacter sphaeroides.
  Journal
J Bacteriol 179:7462-7 (1997)
DOI:10.1128/JB.179.23.7462-7467.1997
  Sequence
[rsp:RSP_0266]
Other DBs
ExplorEnz - The Enzyme Database: 1.3.99.27
IUBMB Enzyme Nomenclature: 1.3.99.27
ExPASy - ENZYME nomenclature database: 1.3.99.27
BRENDA, the Enzyme Database: 1.3.99.27

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