KEGG ENZYME: 1.3.99.32

ENZYME: 1.3.99.32

Entry

EC 1.3.99.32 Enzyme

Name

glutaryl-CoA dehydrogenase (acceptor);
GDHDes;
nondecarboxylating glutaryl-coenzyme A dehydrogenase;
nondecarboxylating glutaconyl-coenzyme A-forming GDH;
glutaryl-CoA dehydrogenase (non-decarboxylating)

Class

Oxidoreductases;
Acting on the CH-CH group of donors;
With unknown physiological acceptors

Sysname

glutaryl-CoA:acceptor 2,3-oxidoreductase (non-decarboxylating)

Reaction(IUBMB)

glutaryl-CoA + acceptor = (E)-glutaconyl-CoA + reduced acceptor [RN:R05579]

Reaction(KEGG)

R05579

Substrate

glutaryl-CoA [CPD:C00527];
acceptor [CPD:C00028]

Product

(E)-glutaconyl-CoA [CPD:C02411];
reduced acceptor [CPD:C00030]

Comment

The enzyme contains FAD. The anaerobic, sulfate-reducing bacterium Desulfococcus multivorans contains two glutaryl-CoA dehydrogenases: a decarboxylating enzyme (EC 1.3.8.6), and a nondecarboxylating enzyme (this entry). The two enzymes cause different structural changes around the glutaconyl carboxylate group, primarily due to the presence of either a tyrosine or a valine residue, respectively, at the active site.

History

EC 1.3.99.32 created 2012, modified 2013

Pathway

ec00362

Benzoate degradation

ec01100

Metabolic pathways

ec01120

Microbial metabolism in diverse environments

Orthology

K16173

glutaryl-CoA dehydrogenase (non-decarboxylating)

Genes

GBM:

Gbem_1452

GEO:

Geob_0237

GEM:

GM21_2801

DML:	Dmul_25030(acdA5)

DAL:

Dalk_0058

DAT:	HRM2_38550(acd11) HRM2_38670(acd12)

DTO:	TOL2_C38270(acdA5)

SAT:

SYN_00480

DTI:	Desti_2252 Desti_4873

DBR:

Deba_2447

BMYC:

DJ92_4005

BAG:

Bcoa_0791

BSM:	BSM4216_2477

GWC:	GWCH70_0441

GMC:	GY4MC1_3338

GTH:	Geoth_3392

AFL:	Aflv_1240(acdA)

AGN:	AFK25_08310

HMN:	HM131_13220

DKU:	Desku_2606

DGI:	Desgi_4610

DOR:	Desor_1180 Desor_2984

DAI:	Desaci_1830

CHY:

CHY_1737

» show all

Reference

1 [PMID:19395484]

Authors

Wischgoll S, Taubert M, Peters F, Jehmlich N, von Bergen M, Boll M

Title

Decarboxylating and nondecarboxylating glutaryl-coenzyme A dehydrogenases in the aromatic metabolism of obligately anaerobic bacteria.

Journal

J Bacteriol 191:4401-9 (2009)
DOI:10.1128/JB.00205-09

Sequence

[ag:ACP50614]

Reference

2 [PMID:20486657]

Authors

Wischgoll S, Demmer U, Warkentin E, Gunther R, Boll M, Ermler U

Title

Structural basis for promoting and preventing decarboxylation in glutaryl-coenzyme a dehydrogenases.

Journal

Biochemistry 49:5350-7 (2010)
DOI:10.1021/bi100317m

Sequence

[ag:ACP50614]

Other DBs

ExplorEnz - The Enzyme Database:

1.3.99.32

IUBMB Enzyme Nomenclature:

1.3.99.32

ExPASy - ENZYME nomenclature database:

1.3.99.32

BRENDA, the Enzyme Database:

1.3.99.32

DBGET integrated database retrieval system