KEGG   ENZYME: 1.3.99.5
Entry
EC 1.3.99.5                 Enzyme                                 

Name
3-oxo-5alpha-steroid 4-dehydrogenase (acceptor);
steroid 5alpha-reductase;
3-oxosteroid Delta4-dehydrogenase;
3-oxo-5alpha-steroid Delta4-dehydrogenase;
steroid Delta4-5alpha-reductase;
Delta4-3-keto steroid 5alpha-reductase;
Delta4-3-oxo steroid reductase;
Delta4-3-ketosteroid5alpha-oxidoreductase;
Delta4-3-oxosteroid-5alpha-reductase;
3-keto-Delta4-steroid-5alpha-reductase;
5alpha-reductase;
testosterone 5alpha-reductase;
4-ene-3-ketosteroid-5alpha-oxidoreductase;
Delta4-5alpha-dehydrogenase;
3-oxo-5alpha-steroid:(acceptor) Delta4-oxidoreductase;
tesI (gene name)
Class
Oxidoreductases;
Acting on the CH-CH group of donors;
With unknown physiological acceptors
Sysname
3-oxo-5alpha-steroid:acceptor Delta4-oxidoreductase
Reaction(IUBMB)
a 3-oxo-5alpha-steroid + acceptor = a 3-oxo-Delta4-steroid + reduced acceptor [RN:R02643]
Reaction(KEGG)
R02643 > R01834 R04827
Substrate
3-oxo-5alpha-steroid [CPD:C02940];
acceptor [CPD:C00028]
Product
3-oxo-Delta4-steroid [CPD:C00619];
reduced acceptor [CPD:C00030]
Comment
A flavoprotein. This bacterial enzyme, characterized from Comamonas testosteroni, is involved in androsterone degradation. cf. EC 1.3.1.22, 3-oxo-5alpha-steroid 4-dehydrogenase (NADP+).
History
EC 1.3.99.5 created 1965, modified 2012
Pathway
ec00984  Steroid degradation
ec01120  Microbial metabolism in diverse environments
Orthology
K16051  3-oxo-5alpha-steroid 4-dehydrogenase
Genes
RGL: CS053_01900
PRE: PCA10_43670
PPUT: L483_14100
PPUN: PP4_27190
PPUU: PputUW4_01680
PKC: PKB_4321
PFZ: AV641_10570
PLQ: AA042_20375
PPSY: AOC04_07870
PSET: THL1_4409
PSIL: PMA3_11575
PHA: PSHAa2146
GPB: HDN1F_19870(tesI)
REH: H16_B0641(h16_B0641)
BCEN: DM39_3421
BCEO: I35_5434
BCED: DM42_6385
BCON: NL30_04390
BTEI: WS51_05515
BPSL: WS57_06230
BMEC: WJ16_26540
BFN: OI25_4613
CABA: SBC2_48770
CTES: O987_07765
GLO: Glov_1298
SPMI: K663_02990
SPHB: EP837_01509(tesI)
SFLA: SPHFLASMR4Y_02380(ifcA)
DHD: Dhaf_0773
MTV: RVBD_1817
MRA: MRA_1829
MTUR: CFBS_1909
MTD: UDA_1817
MTUC: J113_12610
MTUE: J114_09700
MTUH: I917_12910
MTUL: TBHG_01774
MTUT: HKBT1_1906
MTUU: HKBT2_1914
MBB: BCG_1852
MBT: JTY_1836
MAF: MAF_18390
MMIC: RN08_2021
MIT: OCO_28580
MIA: OCU_28450
MID: MIP_03716
MYO: OEM_27040
MMM: W7S_13850
MHAD: B586_11860
MTHN: 4412656_00946(ifcA_1) 4412656_02236(ifcA_2)
MHAS: MHAS_01796(fccA) MHAS_02476
MAB: MAB_0623
NFR: ERS450000_00982(ifcA_1) ERS450000_03773(ifcA_3) ERS450000_05269(ifcA_4)
ROP: ROP_57660
RRT: 4535765_00633(ifcA_1) 4535765_00838(ifcA_2)
RCR: NCTC10994_01879(ifcA_1) NCTC10994_02087(ifcA_2) NCTC10994_03292(ifcA_5)
GOR: KTR9_4518
GRU: GCWB2_04130(ifcA1) GCWB2_22595(ifcA3)
GOM: D7316_02580(urdA) D7316_03032(ifcA)
TPR: Tpau_3846
SALU: DC74_8142
SALL: SAZ_42040
NCA: Noca_0296
NDK: I601_0577(urdA) I601_1513(ifcA_1)
TCU: Tcur_3515
FAL: FRAAL3038
AMD: AMED_2521
AMN: RAM_12815
AMM: AMES_2493
AMZ: B737_2494
AOI: AORI_2483
AMYY: YIM_14445 YIM_36750(urdA)
KAL: KALB_5139
ALO: CRK59602
SAQ: Sare_2883
 » show all
Reference
1  [PMID:13673006]
  Authors
LEVY HR, TALALAY P.
  Title
Bacterial oxidation of steroids. II. Studies on the enzymatic mechanism of ring A dehydrogenation.
  Journal
J Biol Chem 234:2014-21 (1959)
Reference
2  [PMID:8655514]
  Authors
Florin C, Kohler T, Grandguillot M, Plesiat P
  Title
Comamonas testosteroni 3-ketosteroid-delta 4(5 alpha)-dehydrogenase: gene and protein characterization.
  Journal
J Bacteriol 178:3322-30 (1996)
DOI:10.1128/JB.178.11.3322-3330.1996
  Sequence
Reference
3  [PMID:12902225]
  Authors
Horinouchi M, Hayashi T, Yamamoto T, Kudo T
  Title
A new bacterial steroid degradation gene cluster in Comamonas testosteroni TA441  which consists of aromatic-compound degradation genes for seco-steroids and 3-ketosteroid dehydrogenase genes.
  Journal
Appl Environ Microbiol 69:4421-30 (2003)
DOI:10.1128/AEM.69.8.4421-4430.2003
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.3.99.5
IUBMB Enzyme Nomenclature: 1.3.99.5
ExPASy - ENZYME nomenclature database: 1.3.99.5
BRENDA, the Enzyme Database: 1.3.99.5
CAS: 9036-43-5

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