KEGG   ENZYME: 1.3.99.8
Entry
EC 1.3.99.8                 Enzyme                                 
Name
2-furoyl-CoA dehydrogenase;
furoyl-CoA hydroxylase;
2-furoyl coenzyme A hydroxylase;
2-furoyl coenzyme A dehydrogenase;
2-furoyl-CoA:(acceptor) 5-oxidoreductase (hydroxylating)
Class
Oxidoreductases;
Acting on the CH-CH group of donors;
With unknown physiological acceptors
Sysname
2-furoyl-CoA:acceptor 5-oxidoreductase (hydroxylating)
Reaction(IUBMB)
2-furoyl-CoA + H2O + acceptor = S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor [RN:R02987]
Reaction(KEGG)
R02987
Substrate
2-furoyl-CoA [CPD:C00845];
H2O [CPD:C00001];
acceptor [CPD:C00028]
Product
S-(5-hydroxy-2-furoyl)-CoA [CPD:C03724];
reduced acceptor [CPD:C00030]
Comment
A copper protein. The oxygen atom of the -OH produced is derived from water, not O2; the actual oxidative step is probably dehydrogenation of a hydrated form -CHOH-CH2- to -C(OH)=CH-, which tautomerizes non-enzymically to -CO-CH2-, giving (5-oxo-4,5-dihydro-2-furoyl)-CoA. Methylene blue, nitro blue, tetrazolium and a membrane fraction from Pseudomonas putida can act as acceptors.
History
EC 1.3.99.8 created 1976
Pathway
ec00365  Furfural degradation
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K16877  2-furoyl-CoA dehydrogenase large subunit
K16878  2-furoyl-CoA dehydrogenase FAD binding subunit
K16879  2-furoyl-CoA dehydrogenase 2Fe-2S iron sulfur subunit
Genes
PPSEBN5_2298(hmfA) BN5_2299(hmfB) BN5_2300(hmfC)
PMANOU5_0887 OU5_0888 OU5_0889
PPUUPputUW4_02623 PputUW4_02624 PputUW4_02625
PSILPMA3_09770 PMA3_09775 PMA3_09780
PALLUYA_10530 UYA_10535 UYA_10540
PSTTCH92_11530 CH92_11535 CH92_11540
HSVHNO53_04195 HNO53_04200 HNO53_04205
HPROLMS44_09430 LMS44_09435 LMS44_09440
ADIB5T_02845 B5T_02846 B5T_02847
RPIRpic_2052 Rpic_2053 Rpic_2054
RPFRpic12D_1744 Rpic12D_1745 Rpic12D_1746
RPJN234_37060 N234_37065 N234_37070
REHH16_A1701(coxS3) H16_A1702(h16_A1702) H16_A1703(coxL3)
CUHBJN34_36275 BJN34_36280 BJN34_36285
REUReut_C5888 Reut_C5889 Reut_C5890
COXE0W60_18630 E0W60_18635 E0W60_18640 E0W60_32415 E0W60_32420
BUGBC1001_3787 BC1001_3788 BC1001_3789
BGFBC1003_0590 BC1003_0591 BC1003_0592
BYIBYI23_A013020 BYI23_A013030 BYI23_A013040
BUQAC233_20840 AC233_20845 AC233_20850
BUMAXG89_18115 AXG89_18120 AXG89_18125 AXG89_31120
BUIAX768_27890 AX768_27895 AX768_27900
BXEBxe_A2073 Bxe_A2074 Bxe_A2075
BXBDR64_4228 DR64_4229 DR64_4230(qorS)
BPHBphy_6671 Bphy_6672 Bphy_6673
BGEBC1002_5745 BC1002_5746 BC1002_5747
BPXBUPH_00361 BUPH_00362 BUPH_00363
BPYBphyt_2183 Bphyt_2184 Bphyt_2185
BUZAYM40_12165 AYM40_12170 AYM40_12175
BFNOI25_3188 OI25_3189 OI25_3190
BCAIK788_0001305 K788_0001306 K788_0001307
PARBCJU94_33600 CJU94_33605 CJU94_33610
PHSC2L64_39380 C2L64_39385 C2L64_39390
PTERC2L65_36660 C2L65_36665 C2L65_36670
PGPCUJ91_22575 CUJ91_22580 CUJ91_22585
PCJCUJ87_08680 CUJ87_08685
PDIOPDMSB3_2266(cutC) PDMSB3_2267 PDMSB3_2268
PDQCL55_00008510 CL55_00008520 CL55_00008530
CABASBC2_58950 SBC2_58960(cdhB_2) SBC2_58970(cdhC)
BPEBP0684A
BPCBPTD_0692
BPERBN118_3020
BPETB1917_0471
BPEUQ425_4310
BPARBN117_0386
BPABPP0389
BBHBN112_3022
BBRBB0391
BBMBN115_0376
BBXBBS798_0387
BAVBAV2737(cutS)
BBROBAU06_03095 BAU06_03100 BAU06_03105 BAU06_14970 BAU06_14975
BFZBAU07_13315 BAU07_13320 BAU07_17240 BAU07_17245 BAU07_17250
BOHAKI39_05190 AKI39_05195 AKI39_05200
BGMCAL15_18105
ACHRC2U31_19620
AKATKWG_13615
AMIMMIM_c34420 MIM_c34430 MIM_c34440
AFAUZ73_14445
ODIODI_R0528 ODI_R0529 ODI_R0530
PIGEGT29_14620 EGT29_14625 EGT29_14630
ACKC380_16250 C380_16255 C380_21260 C380_21265 C380_21270
LIML103DPR2_01708(cutL_3) L103DPR2_01710(kdhA)
HPSEHPF_15720(cdhA) HPF_15725(kdhA) HPF_15730(cdhC)
HCZG9Q37_00905 G9Q37_00910 G9Q37_00915 G9Q37_18270
ATERMW290_03380 MW290_03385 MW290_03390
ATWC0099_09885 C0099_09890 C0099_09895
MLNA9174_34060 A9174_34085
ORMHTY61_00440 HTY61_00445 HTY61_00450
KMNHW532_18830 HW532_18835
EAKEKH55_3886 EKH55_3887
REIIE4771_PA00054
RGARGR602_PA00038(coxM-2)
RHNAMJ98_PC00054
REZAMJ99_PB00053
ABAWD5400_18155 D5400_18160 D5400_18165
BJAblr3533(cutL) blr3534(cutM) blr3535(blr3535)
BJUBJ6T_61950 BJ6T_61960 BJ6T_61970
BJPRN69_30010 RN69_30015 RN69_30020
BOTCIT37_36540 CIT37_36545 CIT37_36550
BGQX265_16165 X265_16170 X265_16175
BARHWN72_17955 WN72_17960 WN72_17965
BELBE61_31160(coxM)
RPCRPC_0848 RPC_0849 RPC_0850
BOSBSY19_1058 BSY19_1059 BSY19_1060
BVVBHK69_28550 BHK69_28555 BHK69_28560
BOFFQV39_09960
XDIEZH22_10480 EZH22_10485 EZH22_10490
MRDMrad2831_4723 Mrad2831_4724 Mrad2831_4725
METM446_1301 M446_1302 M446_1303
MNOMnod_3296 Mnod_3297 Mnod_3298
MORMOC_3637 MOC_3638 MOC_3639
MAQUMaq22A_c13985(coxS) Maq22A_c13990(coxM) Maq22A_c13995(coxL)
MPHYMCBMB27_05472 MCBMB27_05473 MCBMB27_05474
MEEDA075_33360 DA075_33365 DA075_33370
METXA3862_04985 A3862_04990 A3862_04995
METIDK427_10980 DK427_10985 DK427_10990
RHZRHPLAN_07920 RHPLAN_07930 RHPLAN_07940 RHPLAN_53800
PSINCAK95_25375 CAK95_25380 CAK95_25385 CAK95_28845 CAK95_28855
RBMTEF_08695
DSHDshi_3719(coxL) Dshi_3720(coxM) Dshi_3721(coxS)
PABYGa0080574_TMP3693 Ga0080574_TMP3694 Ga0080574_TMP3695
SULDB5M07_03660 B5M07_03665 B5M07_03670
SPOTG6548_00070 G6548_00075 G6548_00080
RIDRIdsm_04272(cdhA) RIdsm_04273(cdhB) RIdsm_04274(cdhC_3)
PTPRCA23_c12060 RCA23_c12070 RCA23_c12080
CMAGCBW24_14190 CBW24_14195 CBW24_14200
SMICSmB9_31320
ACRAcry_0668 Acry_0669 Acry_0670
AMVACMV_11990 ACMV_12000 ACMV_12010
AZLAZL_a11000 AZL_a11010(cutM) AZL_a11020(xdhB)
ATIAL072_24270 AL072_24275 AL072_24280
AOZHUE56_07955 HUE56_07960 HUE56_07965
BACQDOE78_11790 DOE78_11800
BKOCKF48_20745
CFIRNAF01_14290
GKAGKP19 GKP21
GMCGY4MC1_2423 GY4MC1_2425
GGHGHH_c13560(cutL) GHH_c13580
PTLAOT13_10300 AOT13_10310
PCALBV455_00386(kdhA) BV455_00388(cdhA)
PARGPspKH34_23880 PspKH34_23900(cutL)
ANMGFC28_297 GFC28_299
ANLGFC29_1325 GFC29_1327
APAKAP3564_10395 AP3564_10405
SHVAAT16_04525 AAT16_04535
PBJVN24_01240
PNPIJ22_36520 IJ22_36540
KYRCVV65_11710
EFFskT53_32220(cutL) skT53_32240
SAYTPY_2900(cutM)
SAPSulac_0987
MDXBTO20_26750
MGORH0P51_10250
MALVMALV_28090
SVLStrvi_4351 Strvi_4353
SRCM271_02660 M271_02670
PSEIGCE65_15275
JAYH7A72_04100 H7A72_12330 H7A72_12340
BLUTEW640_00545
NCANoca_1489
NDKI601_1311(kdhA)
NOYEXE57_06220
NANOG5V58_15385
NPSKRR39_18325 KRR39_18335
PSIMKR76_07345
NOABKM31_01510
NOWGBF35_36485
BLAPMVA48_02295 MVA48_10745 MVA48_10755
SACCEYD13_13315(kdhA)
AMQAMETH_3079 AMETH_3081(cutM)
AACDLWP59_20645 LWP59_20655
PDXPsed_5931 Psed_5933 Psed_6475
PSEAWY02_08300 WY02_08310 WY02_22155
PSEEFRP1_20535
PSEHXF36_05370 XF36_23380
PSEQAD006_27955
PECQAD017_07550
PHHAFB00_17905
PAUTPdca_02880 Pdca_13730 Pdca_67730(cutL_4) Pdca_67750
PBROHOP40_06535 HOP40_06545
EKEEK0264_00850
SBAEDSM104329_02310(cutS_1) DSM104329_04642(cutL_4)
KBSEPA93_17140
HDLHALDL1_11485
HALJG9465_23325
HWAHQ_1944A(coxL)
SRUBC2R22_07980
HMPK6T50_16000
HREK6T36_15890
HAHHalar_1108
NVRFEJ81_19730
HLRHALLA_02370
HLCCHINAEXTREME03555
STOSTK_00470
SOHD1869_14670 D1869_14875
SISLS215_0755
SIAM1425_0853
SIMM1627_0918
SIDM164_0871
SIYYG5714_0773
SIHSiH_0725
SULABFU36_07620
SULEGFS03_05235
AMANB6F84_00990
ASULDFR86_00545
SACDHS1genome_0454 HS1genome_0527
PYRP186_1143
POGPogu_1990
VDIVdis_0419
VMOVMUT_1125
 » show all
Reference
1  [PMID:4655411]
  Authors
Kitcher JP, Trudgill PW, Rees JS.
  Title
Purification and properties of 2-furoyl-coenzyme A hydroxylase from Pseudomonas putida F2.
  Journal
Biochem J 130:121-32 (1972)
DOI:10.1042/bj1300121
Other DBs
ExplorEnz - The Enzyme Database: 1.3.99.8
IUBMB Enzyme Nomenclature: 1.3.99.8
ExPASy - ENZYME nomenclature database: 1.3.99.8
UM-BBD (Biocatalysis/Biodegradation Database): 1.3.99.8
BRENDA, the Enzyme Database: 1.3.99.8
CAS: 9068-18-2

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