KEGG ENZYME: 1.4.1.14

ENZYME: 1.4.1.14

Entry

EC 1.4.1.14                 Enzyme

Name

glutamate synthase (NADH);
glutamate (reduced nicotinamide adenine dinucleotide) synthase;
NADH: GOGAT;
L-glutamate synthase (NADH);
L-glutamate synthetase;
NADH-glutamate synthase;
NADH-dependent glutamate synthase

Class

Oxidoreductases;
Acting on the CH-NH2 group of donors;
With NAD+ or NADP+ as acceptor

Sysname

L-glutamate:NAD+ oxidoreductase (transaminating)

Reaction(IUBMB)

2 L-glutamate + NAD+ = L-glutamine + 2-oxoglutarate + NADH + H+ [RN:R00093];
(1a) L-glutamate + NH3 = L-glutamine + H2O [RN:R00256];
(1b) L-glutamate + NAD+ + H2O = NH3 + 2-oxoglutarate + NADH + H+ [RN:R00243]

Reaction(KEGG)

R00093 R00243 R00256

Substrate

L-glutamate [CPD:C00025];
NAD+ [CPD:C00003];
NH3 [CPD:C00014];
H2O [CPD:C00001]

Product

L-glutamine [CPD:C00064];
2-oxoglutarate [CPD:C00026];
NADH [CPD:C00004];
H+ [CPD:C00080];
H2O [CPD:C00001];
NH3 [CPD:C00014]

Comment

A flavoprotein (FMN). The reaction takes place in the direction of L-glutamate production. The protein is composed of two domains, one hydrolysing L-glutamine to NH3 and L-glutamate (cf. EC 3.5.1.2, glutaminase), the other combining the produced NH3 with 2-oxoglutarate to produce a second molecule of L-glutamate (cf. EC 1.4.1.2, glutamate dehydrogenase).

History

EC 1.4.1.14 created 1978, modified 2019

Pathway

ec00250

Alanine, aspartate and glutamate metabolism

ec00910

Nitrogen metabolism

ec01100

Metabolic pathways

ec01110

Biosynthesis of secondary metabolites

ec01120

Microbial metabolism in diverse environments

Orthology

K00264

glutamate synthase (NADH)

Genes

BFO:

118423229

BBEL:

109473320

SPU:

754163

LPIC:

129258831 129258833 129259375 129259376

ARUN:

117296435

AJC:

117119745

DME:	Dmel_CG9674(CG9674)

DER:

6543630

DSE:

6606013

DSI:	Dsimw501_GD12441(Dsim_GD12441)

DYA:	Dyak_GE22188

DAN:

6493372

DSR:

110190291

DPO:

4812304

DPE:

6601003

DMN:

108152363

DWI:

6645095

DGR:

6557958

DMO:	Dmoj_GI11930

DAZ:

108613420

DNV:

108650790

DHE:

111605684

DVI:

6622079

CCAT:

101448290

BOD:

106620726

BDR:

105232888

RZE:	108360908 108371738

AOQ:

129242928

TDA:

119668579

MDE:	101893373 131804054

SCAC:

106092120

LCQ:

111683468

LSQ:

119602600

GFS:

119632430

ECOE:

129946950

CLON:

129610451 129618227

HIS:

119650296

AGA:

1276969

ACOZ:

120951886

AARA:

120895665

AMER:

121593754

ASTE:

118509523

AFUN:

125772032

AMOU:

128305499

AALI:

118465043

AAG:

5565246

AALB:

109410517

ASUA:

134209681

CQU:	CpipJ_CPIJ007345

CPII:

120430753

CNS:

116345940

BCOO:

119080661

LLL:

129792715

PPAP:

129801393

AME:

413372

ACER:

108004089

ALAB:

122714169

ADR:

102678387

AFLR:

100863333

BIM:

100743936

BBIF:

117208172

BVK:

117235398

BVAN:

117154250

BHUN:

126864791

BTER:

100648506

BAFF:

126916388

BPYO:

122565625

BPAS:

132905378

FVI:

122535197

CCAL:

108629149

OBB:

114879469

OLG:

117610793

MGEN:

117218009

NMEA:

116432027

CGIG:

122398534

SOC:

105197574

MPHA:

105838501

AEC:

105149802

ACEP:

105627138

PBAR:

105424147

VEM:

105559493

HST:

105181045

DQU:

106749864

CFO:

105259420

FEX:

115245214

LHU:

105677426

PGC:

109859877

OBO:

105286430

PCF:

106785427

PFUC:

122514263

VPS:

122634919

VCRB:

124430514

VVE:

124954153

NVI:

100122109

CSOL:

105368803

TPRE:

106656707

LHT:

122508094

LBD:

127280028

MDL:

103570096

CGLO:

123272834

FAS:

105270599

DAM:

107043748

AGIF:

122853354

CINS:

118074872

VCAN:

122411181

CCIN:

107270167

DSM:

124416509

NPT:

124224641

NFB:

124182271

NLO:

107216749

NVG:

124304630

AROA:

105689110

TCA:

658584

DPA:	109543624 125503707

SOY:

115886449

AGRG:

126745911

ATD:

109601527

CSET:

123312571

AGB:

108917077

LDC:

111508893

DVV:

114336495

NVL:

108563867

DCOR:

135796622

APLN:

108732952

PPYR:

116171129

OTU:

111413251

MSEX:

115452078

DPL:	KGM_210060

BANY:

112047329

MJU:

123866946

NIQ:

126768611

VCD:

124530588

MCIX:

123654185

PPOT:

106100510

PXU:

106125288

PRAP:

110993503

PBX:

123714623

PNAP:

125060109

ZCE:

119831402

CCRC:

123692791

LSIN:

126973096

AAGE:

121731315

HZE:

124630844

SLIU:

111349578

OFU:

114358173

PGW:

126367002

CFEL:

113378636

CCRN:

123290403

API:

100158883

DNX:

107164646

AGS:

114126871

RMD:

113555018

ACOO:

126832971

DVT:

126906074

BTAB:

109029618

DCI:

103524615

CLEC:

106667559

HHAL:

106690746

NLU:

111052344

HVI:

124358254

MQU:

128983935

FOC:

113206005

TPAL:

117639366

ZNE:

110836732

CSEC:

111865708

SGRE:

126284988

BROR:

134529642

IEL:

124160182

DPX:	DAPPUDRAFT_110162

DMK:

116920522

DPZ:

124316122

PVM:

113799962

PJA:

122255183

PCHN:

125045218

PMOO:

119584663

HAME:

121879794

PCLA:

123755771

CQD:

128704206

PTRU:

123520672

ESN:

126998347

MNZ:

135199441

MRJ:

136851520

HAZT:

108678918

EAF:

111704323

LSM:

121128450

TCF:

131887265

PPOI:

119102368 119102488

ISC:

120849612

DSV:

119461673

RSAN:

119395866

RMP:

119174150

VDE:

111247835

VJA:

111272506

DPTE:

113798827

DFR:

124499735

CSCU:

111624033 111638555

CVS:	136972973 136977827

PTEP:

107440837

ABRU:

129975231 129984806

UDV:	129224565 129232157

SDM:	118187282 118187991

LPOL:

106459606

CEL:	CELE_W07E11.1(W07E11.1)

CBR:

CBG_01975

CRQ:	GCK72_023520

PVUL:

126810534

BGT:

106056462

GAE:

121371892

HRF:

124116337

HRJ:

124282667

HASI:

137288160

CRG:

105337796

CANU:

128186176

CVN:	111100398 111100399

OED:

125673905

MYI:

110451591

PMAX:

117327627

AIRR:

138330850

MMER:

123552518

RPHI:

132756035

DPOL:

127852221

MAEA:

128237296

OBI:

106883777

OSN:

115223129

LJP:

135481753

LLON:

135487499

MCAZ:

138052646

MFOO:

137996932

XEN:

124443157

RES:

135685722

ATH:	AT5G53460(GLT1)

ALY:

9300320

CRB:

17876423

CSAT:

104725863 104728554 104761363

EUS:	EUTSA_v10012414mg

BRP:

103857079

BNA:	106387482 111213076

BOE:

106332590

RSZ:

108845736

THJ:

104811925

CPAP:

110823143

CIT:

102620225

PVY:

116133280

MINC:

123198124 123206665 123226207

TCC:

18605743

GRA:

105765457

GHI:	107929529 107947250

GAB:

108479092

HSYR:

120117517 120184088 120206192

DZI:	111280486 111289287 111309227

EGR:

104437859

GMX:

100815931

GSJ:

114410308

PVU:	137813561 137822651

VRA:

106764633

VAR:

108331410

VUN:

114194130

VUM:

124822217

CCAJ:

109814577

APRC:

113850812

MTR:

25482265

TPRA:

123902867

CAM:

101509615

PSAT:

127091893

VVO:

131596096

ADU:

107495014

AIP:

107605257

AHF:	112697424 112705922 112755343 112765389

LANG:

109326556

QSA:	O6P43_015015 O6P43_019921

FVE:

101309397

AANS:

126803411

RCN:

112183464

PPER:

18785174

PMUM:

103333633

PAVI:

110767133

PDUL:

117619118

MDM:	103439880 103443818

MSYL:

126624044 126629149

PXB:	103941488 103943433 103967236

ZJU:

107414598

MNT:

21390250

CSAV:

115715577

CSV:

101213224

CMO:

103485701

BHJ:

120081937

MCHA:

111011404

CMAX:

111482791

CMOS:

111430634

CPEP:

111810135

RCU:

8274353

MEAN:

126671937

JCU:

105648339

HBR:	110665757 110666168

MESC:

110617349 110630652

POP:	18103637 7453835

PEU:	105109026 105110510

PALZ:

118033501 118057296

PNZ:	133676101 133678545

JRE:	109004582 109013214

CILL:

122311011 122312965

CAVE:

132162263 132165333

AGLU:

133857515

QSU:

112017959

QLO:

115959755

TWL:	119984320 120003889

VVI:

100246868

VRI:

117933639

SLY:

101254281

SPEN:

107013700

SOT:

102580516

SSTN:

125858190

SDUL:

129894061

CANN:

107855963

LBB:

132632063

NSY:

104244568

NAU:

109213942

INI:

109183410

ITR:

116012569

SIND:

105160371

OEU:

111405628

EGT:

105971643

SSPL:

121747965 121752173 121779747 121782410

SMIL:

131014672

SHIS:

125213241 125213411

APAN:

127256298 127259584

HAN:	110878172 110912724

ECAD:

122586618

LSV:

111918005

CCAV:

112508668 112515985

DCR:	108214500 108226948

CSIN:

114285508 114310758

RVL:	131301738 131319786 131319792

AEW:	130753473 130767247

BVG:

104888263

SOE:

110783090

CQI:	110696098 110728845

ATRI:

130806671

MOF:

131145391

NNU:	104600364 104604004

MING:

122062882 122081042

TSS:	122640469 122666202

PSOM:

113299164 113358466

OSA:	4324398 4339561(GLT2)

DOSA:

Os01g0681900

OBR:	102713634 102721892

OGL:	127761687 127773744

BDI:	100832857 100844360

ATS:

109760356

TDC:	119268802 119279609

TAES:

123061864 123070558 123078967

LPER:

127343353

LRD:

124660979

SBI:

8068026

ZMA:	103636185 103651348

MFLO:

136450302 136456380

SITA:

101785947

SVS:

117857834

PVIR:

120664696 120675270 120697894 120707627

PHAI:

112894413

PAUA:

133908489 133918129 133929376

PDA:	103708325 103713334

EGU:	105039187 105041491

MUS:	103980032 103996064 135617878

ZOF:	122020245 122025187 122026291 122032959 122033388

DCT:

110110468

PEQ:

110020245

AOF:

109840747

MSIN:

131241230 131245159

ACAK:

137942584 137961875

NCOL:

116266917

ATR:

18442390

CJF:

131065765

SMO:	SELMODRAFT_446658

PPP:

112283025

CRE:	CHLRE_13g592200v5

VCN:	VOLCADRAFT_108038

APRO:

F751_6615

MIS:	MICPUN_105473(GLT)

MPP:	MICPUCDRAFT_57115(GLT)

SCE:	YDL171C(GLT1)

SEUB:

DI49_0907

SPAO:

SPAR_D00710

AGO:	AGOS_ADR290W

ERC:

Ecym_4713

KLA:	KLLA0_E19625g

KMX:	KLMA_50304(GLT1)

LTH:	KLTH0B04708g

VPO:	Kpol_530p21

ZRO:	ZYRO0B02596g

CGR:	2890911(GLT1)

NCS:	NCAS_0A14190(NCAS0A14190)

NDI:	NDAI_0A01860(NDAI0A01860)

TPF:	TPHA_0F03070(TPHA0F03070)

TGB:	HG536_0B05360

TDL:	TDEL_0C01870(TDEL0C01870)

KNG:	KNAG_0C03720(KNAG0C03720)

ZMK:	HG535_0B04610

SLUD:

SCDLUD_000217

DHA:	DEHA2G15642g

PIC:	PICST_87428(GLT1)

SPAA:

SPAPADRAFT_141734(GLT1)

LEL:	PVL30_000542(GLT1)

LBG:	92205742(LODBEIA_P05460)

CAL:	CAALFM_C106550WA(GLT1)

CTP:	CTRG_04234

COT:	CORT_0A06160

CDU:	CD36_06130

CTEN:

18245441(GLT1)

CLU:	CLUG_03565

CLUS:

A9F13_01g06028

CAUR:

CJI96_0003482

PKZ:	C5L36_0E05370

BNN:	FOA43_002177(GLT1)

BBRX:

BRETT_003814

OPA:	HPODL_00613

YLI:	2907005(YALI2_C00682g)

NCR:	NCU01744(en(am)-2)

NTE:	NEUTE1DRAFT65142(NEUTE1DRAFT_65142)

SMP:	10808319(SMAC4_02431)

PAN:	PODANSg6535

PBEL:

QC761_0055180(GLT1_2)

PPSD:

QC762_0055380(GLT1)

PPSP:

QC763_0054760(GLT1)

PPSA:

QC764_0054500(GLT1)

TTT:	THITE_2124366

MTM:	MYCTH_2312662

CTHR:

CTHT_0008270

MGR:

MGG_07187

PPEI:

PpBr36_01824

PGRI:

PgNI_07601

TMN:	UCRPA7_4478

SSCK:

SPSK_08269

FGR:	FGSG_01433

FPU:	FPSE_08520

FPOA:

FPOAC1_001542(GLT1)

FVN:	FVRRES_01748

FVR:	FVEG_09511

FOX:	FOXG_11281

NHE:	NECHADRAFT_57063

FFC:	NCS54_00040100

FKR:	NCS57_00038200

FMU:	J7337_000450(GLT1)

TRE:	TRIREDRAFT_122287(glt1)

TRR:	M419DRAFT_24904

TATV:

25775645(TrAtP1_005625)

TASP:

36612333(GLT1)

MAW:	19244343(GLT1_1)

MAJ:

MAA_01819

MBRN:

26239404(G6M90_00g034430)

PCHM:

VFPPC_01998

CMT:

CCM_03495

AMUS:

LMH87_010616

PLJ:	28889325(PLICBS_008229)

PTKZ:

JDV02_006427(GLT1)

CFJ:	CFIO01_07667

CLUP:

CLUP02_00774

CHIG:

CH63R_12724

CDET:

87942897(CDEST_06394)

SAPO:

SAPIO_CDS0981

ELA:	UCREL1_4299

PFY:	PFICI_11366

SSL:	SS1G_07849

BFU:	BCIN_15g02460(Bcglt1)

MBE:

MBM_04374

PSCO:

LY89DRAFT_691010

GLZ:	GLAREA_03052

ANI:	ANIA_05134

AFM:	AFUA_1G07380

ACT:	ACLA_027210

NFI:	NFIA_017350

AOR:	AO090012000988

ANG:	An07g09920

AFV:	AFLA_002716

ALUC:

AKAW2_11294S(GLT1)

APUU:

APUU_41225A(GLT1)

PCS:	N7525_005197

PDP:	PDIP_26070

POU:	POX_e06407

TMF:	EYB26_006835

TRG:	TRUGW13939_11475

CIM:	CIMG_02486

CPW:	9696482(GLT1)

URE:	UREG_03225

ABE:

ARB_04215

TVE:

TRV_01094

PBL:	PAAG_12483(PAAG_07998)

PBN:	PADG_02101

BGH:	BDBG_06065

PTE:

PTT_08692

PTRR:

6342223(PtrM4_092930)

BZE:	COCCADRAFT_4567

BSC:	COCSADRAFT_185254

BOR:	COCMIDRAFT_10233

AALT:

CC77DRAFT_261749

ARAB:

EKO05_0008651

ZTR:	MYCGRDRAFT_110982

PFJ:	MYCFIDRAFT_52899

FFU:	CLAFUR5_03176

CBET:

CB0940_03694

BCOM:

BAUCODRAFT_475239

NPA:	UCRNP2_1000

TML:	GSTUM_00002450001

SPO:	2543509(glt1)

SOM:	SOMG_00784(glt1)

CNE:

CNJ02910

CNB:

CNBJ0620

CNG:	CNAG_04862

CGI:	CGB_I4060C

CDEU:

CNBG_5689

CDEP:

91088717(L203_104507)

KMG:	30161597(I203_107037)

KNE:	92182689(IAR55_005431)

TMS:	TREMEDRAFT_42578

TASA:

A1Q1_06977

CCAC:

CcaHIS019_0602820(GLT1)

TVS:	TRAVEDRAFT_27775

DSQ:	DICSQDRAFT_177406

PCO:	PHACADRAFT_127541

SHS:	STEHIDRAFT_74778

HIR:	HETIRDRAFT_433732

PSQ:	PUNSTDRAFT_144302

ADL:	AURDEDRAFT_114408

FME:	FOMMEDRAFT_166121

GTR:	GLOTRDRAFT_137109

RSX:	RhiXN_02756

LBC:	LACBIDRAFT_183838

CCI:	CC1G_13933

ABP:	AGABI1DRAFT104661(AGABI1DRAFT_104661)

ABV:	AGABI2DRAFT176670(AGABI2DRAFT_176670)

PCUB:

JR316_0003626

MRR:	Moror_14828

E1B28_002588(GLT1)

SCM:	SCHCO_02607326(SCHCODRAFT_02607326)

CPUT:

CONPUDRAFT_80026

SLA:	SERLADRAFT_357441(NADH-GOGAT)

UMA:	UMAG_03850

PFP:	PFL1_06431

SGRA:

EX895_001280

MGL:

MGL_2017

MRT:

MRET_1549

MSYM:

MSY001_1099

PGR:	PGTG_15142

PTRC:

PtA15_6A702

PSTR:

Pst134EA_002810

MLR:	MELLADRAFT_48761

RIE:	OCT59_005483

MBR:	MONBRDRAFT_15256

PFA:	PF3D7_1435300

PFD:	PFDG_02810

PFH:	PFHG_00264

PREI:

PRSY57_1434600

PGAB:

PGSY75_1435300

PLAG:

39744291(PADL01_1435300)

PYO:	PY17X_1011100

PCB:	PCHAS_1010400

PBE:	PBANKA_1009500

PVV:	PVVCY_0801000

PKN:	PKNH_0421900

PVX:	PVX_084770

PCY:	PCYB_132360

PMAL:

PMUG01_13025000

PBRS:

92372974(MKS88_004887)

PREL:

PRELSG_1315800

PCOT:

PCOAH_00007830

PTI:	PHATRDRAFT_51214(GltX)

TPS:	THAPSDRAFT_29861

» show all

Reference

1 [PMID:4362465]

Authors

Roon RJ, Even HL, Larimore F

Title

Glutamate synthase: properties of the reduced nicotinamide adenine dinucleotide-dependent enzyme from Saccharomyces cerevisiae.

Journal

J Bacteriol 118:89-95 (1974)
DOI:10.1128/JB.118.1.89-95.1974

Reference

2 [PMID:21790]

Authors

Boland MJ, Benny AG.

Title

Enzymes of nitrogen metabolism in legume nodules. Purification and properties of NADH-dependent glutamate synthase from lupin nodules.

Journal

Eur J Biochem 79:355-62 (1977)
DOI:10.1111/j.1432-1033.1977.tb11816.x

Reference

3 [PMID:7047525]

Authors

Masters DS Jr, Meister A

Title

Inhibition of homocysteine sulfonamide of glutamate synthase purified from Saccharomyces cerevisiae.

Journal

J Biol Chem 257:8711-5 (1982)

Reference

4 [PMID:16666762]

Authors

Anderson MP, Vance CP, Heichel GH, Miller SS

Title

Purification and Characterization of NADH-Glutamate Synthase from Alfalfa Root Nodules.

Journal

Plant Physiol 90:351-8 (1989)
DOI:10.1104/pp.90.1.351

Reference

5 [PMID:18182018]

Authors

Blanco L, Reddy PM, Silvente S, Bucciarelli B, Khandual S, Alvarado-Affantranger X, Sanchez F, Miller S, Vance C, Lara-Flores M

Title

Molecular cloning, characterization and regulation of two different NADH-glutamate synthase cDNAs in bean nodules.

Journal

Plant Cell Environ 31:454-72 (2008)
DOI:10.1111/j.1365-3040.2008.01774.x

Other DBs

ExplorEnz - The Enzyme Database:

1.4.1.14

IUBMB Enzyme Nomenclature:

1.4.1.14

ExPASy - ENZYME nomenclature database:

1.4.1.14

BRENDA, the Enzyme Database:

1.4.1.14

CAS:	65589-88-0

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