Oxidoreductases;
Acting on the CH-NH2 group of donors;
With NAD+ or NADP+ as acceptor

L-lysine:NAD+ 6-oxidoreductase (deaminating)

L-lysine + NAD+ = (S)-2,3,4,5-tetrahydropyridine-2-carboxylate + NADH + H+ + NH3 (overall reaction) [RN:R10054];
(1a) L-lysine + NAD+ + H2O = (S)-2-amino-6-oxohexanoate + NADH + H+ + NH3 [RN:R00446];
(1b) (S)-2-amino-6-oxohexanoate = (S)-2,3,4,5-tetrahydropyridine-2-carboxylate + H2O (spontaneous) [RN:R02317]

R00446 R02317 R10054

L-lysine [CPD:C00047];
NAD+ [CPD:C00003];
H2O [CPD:C00001];
(S)-2-amino-6-oxohexanoate [CPD:C04076]

The enzyme is highly specific for L-lysine as substrate, although S-(2-aminoethyl)-L-cysteine can act as a substrate, but more slowly. While the enzyme from Agrobacterium tumefaciens can use only NAD+, that from the thermophilic bacterium Geobacillus stearothermophilus can also use NADP+, but more slowly [1,4].

EC 1.4.1.18 created 1989, modified 2006, modified 2011

1  [PMID:6801024]

Misono H, Nagasaki S.

Occurrence of L-lysine epsilon-dehydrogenase in Agrobacterium tumefaciens.

J Bacteriol 150:398-401 (1982)
DOI:10.1128/JB.150.1.398-401.1982

2

Misono, H., Uehigashi, H., Morimoto, E. and Nagasaki, S.

Purification and properties of L-lysine epsilon-dehydrogenase from Agrobacterium tumefaciens.

Agric Biol Chem 49:2253-2255 (1985)

3  [PMID:2768207]

Misono H, Hashimoto H, Uehigashi H, Nagata S, Nagasaki S.

Properties of L-lysine epsilon-dehydrogenase from Agrobacterium tumefaciens.

J Biochem (Tokyo) 105:1002-8 (1989)
DOI:10.1093/oxfordjournals.jbchem.a122757

4  [PMID:14766574]

Heydari M, Ohshima T, Nunoura-Kominato N, Sakuraba H.

Highly stable L-lysine 6-dehydrogenase from the thermophile Geobacillus stearothermophilus isolated from a Japanese hot spring: characterization, gene cloning and sequencing, and expression.

Appl Environ Microbiol 70:937-42 (2004)
DOI:10.1128/AEM.70.2.937-942.2004

[ag:BAB39707]