Acting on the CH-NH2 group of donors;
With NAD+ or NADP+ as acceptor
L-lysine:NAD+ 6-oxidoreductase (deaminating)
L-lysine + NAD+ = (S)-2,3,4,5-tetrahydropyridine-2-carboxylate + NADH + H+ + NH3 (overall reaction) [RN:
(1a) L-lysine + NAD+ + H2O = (S)-2-amino-6-oxohexanoate + NADH + H+ + NH3 [RN:
(1b) (S)-2-amino-6-oxohexanoate = (S)-2,3,4,5-tetrahydropyridine-2-carboxylate + H2O (spontaneous) [RN:
The enzyme is highly specific for L-lysine as substrate, although S-(2-aminoethyl)-L-cysteine can act as a substrate, but more slowly. While the enzyme from Agrobacterium tumefaciens can use only NAD+, that from the thermophilic bacterium Geobacillus stearothermophilus can also use NADP+, but more slowly [1,4].
EC 18.104.22.168 created 1989, modified 2006, modified 2011
ec00960 Tropane, piperidine and pyridine alkaloid biosynthesis
ec01110 Biosynthesis of secondary metabolites
BLEN: NCTC4824_00341(lys1_1) » show all
Misono H, Nagasaki S.
Occurrence of L-lysine epsilon-dehydrogenase in Agrobacterium tumefaciens.
Misono, H., Uehigashi, H., Morimoto, E. and Nagasaki, S.
Purification and properties of L-lysine epsilon-dehydrogenase from Agrobacterium tumefaciens.
Agric Biol Chem 49:2253-2255 (1985)
Misono H, Hashimoto H, Uehigashi H, Nagata S, Nagasaki S.
Properties of L-lysine epsilon-dehydrogenase from Agrobacterium tumefaciens.
Heydari M, Ohshima T, Nunoura-Kominato N, Sakuraba H
Highly stable L-lysine 6-dehydrogenase from the thermophile Geobacillus stearothermophilus isolated from a Japanese hot spring: characterization, gene cloning and sequencing, and expression.
ExplorEnz - The Enzyme Database: 22.214.171.124
ExPASy - ENZYME nomenclature database: 126.96.36.199