KEGG   ENZYME: 1.4.1.18Help
Entry
EC 1.4.1.18                 Enzyme                                 

Name
lysine 6-dehydrogenase;
L-lysine epsilon-dehydrogenase;
L-lysine 6-dehydrogenase;
LysDH
Class
Oxidoreductases;
Acting on the CH-NH2 group of donors;
With NAD+ or NADP+ as acceptor
BRITE hierarchy
Sysname
L-lysine:NAD+ 6-oxidoreductase (deaminating)
Reaction(IUBMB)
L-lysine + NAD+ = (S)-2,3,4,5-tetrahydropyridine-2-carboxylate + NADH + H+ + NH3 (overall reaction) [RN:R10054];
(1a) L-lysine + NAD+ + H2O = (S)-2-amino-6-oxohexanoate + NADH + H+ + NH3 [RN:R00446];
(1b) (S)-2-amino-6-oxohexanoate = (S)-2,3,4,5-tetrahydropyridine-2-carboxylate + H2O (spontaneous) [RN:R02317]
Reaction(KEGG)
Substrate
L-lysine [CPD:C00047];
NAD+ [CPD:C00003];
H2O [CPD:C00001];
(S)-2-amino-6-oxohexanoate [CPD:C04076]
Product
(S)-2,3,4,5-tetrahydropyridine-2-carboxylate [CPD:C00450];
NADH [CPD:C00004];
H+ [CPD:C00080];
NH3 [CPD:C00014];
(S)-2-amino-6-oxohexanoate [CPD:C04076];
H2O [CPD:C00001]
Comment
The enzyme is highly specific for L-lysine as substrate, although S-(2-aminoethyl)-L-cysteine can act as a substrate, but more slowly. While the enzyme from Agrobacterium tumefaciens can use only NAD+, that from the thermophilic bacterium Geobacillus stearothermophilus can also use NADP+, but more slowly [1,4].
History
EC 1.4.1.18 created 1989, modified 2006, modified 2011
Pathway
ec00960  Tropane, piperidine and pyridine alkaloid biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K19064  lysine 6-dehydrogenase
Genes
PKC: PKB_4254
FGU: SD28_04625
KKO: Kkor_1137
KGE: TQ33_1009
KSD: KS2013_1048
KPD: CW740_05445
PKT: AT984_07930
DAT: HRM2_15290
DOV: DSCO28_39180 DSCO28_43680(lys1) DSCO28_56500 DSCO28_57840
DWD: DSCW_16250 DSCW_58120
MHUA: MCHK_3703
RBS: RHODOSMS8_03240(lysDH)
RLG: Rleg_1836
BACO: OXB_1319
OIH: OB0862
GKA: GK0375
GTN: GTNG_0350
GEA: GARCT_00423(lysDH)
AFL: Aflv_1247
LSP: Bsph_0857
HLI: HLI_20185
VPN: A21D_02343(lysDH)
PSYO: PB01_02625
PSWU: SY83_08020
SIV: SSIL_3578
TMR: Tmar_2139
STED: SPTER_40800(lysDH)
LPIL: LIP_2409
MABB: MASS_0903
NSR: NS506_03769(lys1)
RER: RER_05580
REY: O5Y_02660
SAQ: Sare_0572
GAU: GAU_0501
GBA: J421_2400
SRM: SRM_02773(LYS9)
RMR: Rmar_2611
IAL: IALB_0197
CABY: Cabys_3067
TVO: TVG1335287(TVG1335287)
PHO: PH1688(PH1688)
PYN: PNA2_0280
TON: TON_0429
TGA: TGAM_1420(lys9)
THE: GQS_03055
THA: TAM4_1810
THM: CL1_0259
TLT: OCC_09651
THS: TES1_0147
TNU: BD01_0274
TEU: TEU_07095
PPAC: PAP_01640
MHI: Mhar_0275
TAG: Tagg_0021
HBU: Hbut_0103
PIS: Pisl_1622
PCL: Pcal_0527
PAS: Pars_0657
PYR: P186_2852
POG: Pogu_1699
TNE: Tneu_0647
CMA: Cmaq_1035
TTN: TTX_0809
TUZ: TUZN_0444
VDI: Vdis_0060
VMO: VMUT_1641
ASC: ASAC_0907
ACIA: SE86_05215
BARC: AOA65_1898
BARB: AOA66_0413
LOKI: Lokiarch_02540(lysDH_1) Lokiarch_02550(lysDH_2)
 » show all
Taxonomy
Reference
1  [PMID:6801024]
  Authors
Misono H, Nagasaki S.
  Title
Occurrence of L-lysine epsilon-dehydrogenase in Agrobacterium tumefaciens.
  Journal
J Bacteriol 150:398-401 (1982)
DOI:10.1128/JB.150.1.398-401.1982
Reference
2
  Authors
Misono, H., Uehigashi, H., Morimoto, E. and Nagasaki, S.
  Title
Purification and properties of L-lysine epsilon-dehydrogenase from Agrobacterium tumefaciens.
  Journal
Agric Biol Chem 49:2253-2255 (1985)
Reference
3  [PMID:2768207]
  Authors
Misono H, Hashimoto H, Uehigashi H, Nagata S, Nagasaki S.
  Title
Properties of L-lysine epsilon-dehydrogenase from Agrobacterium tumefaciens.
  Journal
J Biochem (Tokyo) 105:1002-8 (1989)
DOI:10.1093/oxfordjournals.jbchem.a122757
Reference
4  [PMID:14766574]
  Authors
Heydari M, Ohshima T, Nunoura-Kominato N, Sakuraba H.
  Title
Highly stable L-lysine 6-dehydrogenase from the thermophile Geobacillus stearothermophilus isolated from a Japanese hot spring: characterization, gene cloning and sequencing, and expression.
  Journal
Appl Environ Microbiol 70:937-42 (2004)
DOI:10.1128/AEM.70.2.937-942.2004
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.4.1.18
IUBMB Enzyme Nomenclature: 1.4.1.18
ExPASy - ENZYME nomenclature database: 1.4.1.18
BRENDA, the Enzyme Database: 1.4.1.18
CAS: 89400-30-6

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