KEGG   ENZYME: 1.4.1.28
Entry
EC 1.4.1.28                 Enzyme                                 
Name
secondary-alkyl amine dehydrogenase [NAD(P)+];
AmDH (ambiguous);
amine dehydrogenase (ambiguous)
Class
Oxidoreductases;
Acting on the CH-NH2 group of donors;
With NAD+ or NADP+ as acceptor
Sysname
secondary-alkyl amine:NAD(P)+ oxidoreductase (deaminating)
Reaction(IUBMB)
a secondary-alkyl amine + H2O + NAD(P)+ = a ketone + NH3 + NAD(P)H + H+ [RN:R13021 R13022]
Reaction(KEGG)
R13021 R13022
Substrate
secondary-alkyl amine [CPD:C22522];
H2O [CPD:C00001];
NAD+ [CPD:C00003];
NADP+ [CPD:C00006]
Product
ketone [CPD:C01450];
NH3 [CPD:C00014];
NADH [CPD:C00004];
NADPH [CPD:C00005];
H+ [CPD:C00080]
Comment
The enzyme has been shown to react preferentially with short-chain ketones such as cyclohexanone, primary amine groups attached to secondary alkyl groups, or D- and L-amino acids. It also reduces aldehydes to primary amines. Cosubstrate preference depends on the substrate.
History
EC 1.4.1.28 created 2022
Orthology
K25950  secondary-alkyl amine dehydrogenase [NAD(P)+]
Genes
PRGRB151_036990
PALACO695_07830
PHEINCTC12003_03184
PRQCYG50_20115
PRJNCTC6933_03276
PMAGJI723_16295
PHAGPZ638_16715
VITADP71_22650
VSTLVJ81_02535
LHKLHK_02682(dapB-1)
DDSDdes_0477
DFIAXF13_02450
DEFCNY67_12015
CFUSCYFUS_009021
CSOALIS82_07275
BDAFSZ17_12900
PKBB4V02_12565
PTHINDS46_20650
PDYQJQ58_01415
PSPNL1F29_30880
SMUSMU_527
SMCSmuNN2025_1443
SMUTSMUGS5_02315
SMJSMULJ23_1460(dapB1)
SMUASMUFR_0455
SSASSA_2021
SGOSGO_0387
SSRSALIVB_1352
SPATA0O21_00670
SRATFY406_05215
STRGSRT_15900
SFERNCTC12278_01801
SCHJDDV21_002215
SOSINT76_10525
LGNABM34_07565
LDXLH506_05355
EMUEMQU_2449
EAVEH197_22580
VPIBW732_10195
VFVK5K99_01910
GSGCYJ72_005115
GADK8O88_08545
HHJNQ487_07515
FSAC5Q98_04800
RUKA4V00_00735
RGVNQ502_12070
SMANC12CBH8_15580
ACOLK5I23_10270
ELMELI_1411
EMTCPZ25_000470
ELIMB2M23_10835
AWOAwo_c34400(dapB2)
LACYA4V08_05170
CSHClosa_0973
RGNRGna_09095
RLRNQ541_01535
CBOLCGC65_17860
CASPNQ535_13680
LBWC3V36_05155 C3V36_09895
FAAHMPREF0389_01077
CMIUB1H56_09445
TEPTepRe1_0280
TAETepiRe1_0312
HOVEQF90_007930
SPOAEQM13_14710
ERHERH_1328
ERSK210_04990
ERLAOC36_02945
ERIEEI45_00350
EBMSG0102_18650
FITFi14EGH31_22290
ERMEYR00_10680
CMITNQ499_01290
OLSOlsu_0652
TIOINP52_04835
MSMMSMEG_3317(dapB)
MSGMSMEI_3231
MSBLJ00_16480
MSNLI99_16485
MSHLI98_16490
MVQMYVA_1576(dapB)
SANLKZO11_34405
SRCM271_34125
LDNH9L06_04775
BROObrsh051_07550
SACGFDZ84_28340
BLOBL0836
BLJBLD_0569
BLFBLIF_0905
BLLBLJ_0914
BLBBBMN68_575
BLMBLLJ_0783
BLKBLNIAS_01599
BLGBIL_10530
BLZBLGT_04910
BLXGS08_04730
BLABLA_0924(dapB)
BLCBalac_1124
BLTBalat_1124
BBBBIF_02266
BBCBLC1_1080
BNMBALAC2494_00125
BLVBalV_1084
BLWW7Y_1124
BLSW91_1149
BANIBl12_1046
BANLBLAC_05660
BNIBANAN_05535
BANMEN10_05695
BCHOBcFMB_08615
ABAOBEQ56_08085
TPAVHRQ91_01365
SSMSpirs_3711
EMIEmin_0479
EPOEpro_1033
CSOMMKD34_04580
PPIOCE91St28_24130
ARAMKAR29_01470
HARAAArcS_1758
NGENatgr_2708
 » show all
Reference
1
  Authors
Itoh, N., Yachi, C. and Kudome, T.
  Title
Determining a novel NAD+-dependent amine dehydrogenase with a broad substrate range from Streptomyces virginiae IFO 12827: purification and characterization.
  Journal
Journal of Molecular Catalysis B: Enzymatic 10:281-290 (2000)
Reference
2
  Authors
Mayol, O., David, S., Darii, E., Debard, A., Mariage, A., Pellouin, V., Petit, J.L., Salanoubat, M., de Berardinis, V., Zaparucha, A. and Vergne-Vaxelaire, C.
  Title
Asymmetric reductive amination by a wild-type amine dehydrogenase from the thermophilic bacteria Petrotoga mobilis.
  Journal
Catalysis Science & Technology 6:7421-7428 (2016)
Reference
3
  Authors
Mayol, O., Bastard, K., Beloti, L., Frese, A., Turkenburg, J.P., Petit, J.L., Mariage, A., Debard, A., Pellouin, V., Perret, A., de Berardinis, V., Zaparucha, A., Grogan, G. and Vergne-Vaxelaire, C.
  Title
A family of native amine dehydrogenases for the asymmetric reductive amination of ketones.
  Journal
Nature Catalysis 2:324-333 (2019)
Reference
4
  Authors
Lee, S., Jeon, H., Giri, P., Lee, U.J., Jung, H., Lim, S., Sarak, S., Khobragade, T.P., Kim, B.G. and Yun, H.
  Title
The reductive amination of carbonyl compounds using native amine dehydrogenase from Laribacter hongkongensis.
  Journal
Biotechnol Bioprocess Eng 26:384-391 (2021)
Other DBs
ExplorEnz - The Enzyme Database: 1.4.1.28
IUBMB Enzyme Nomenclature: 1.4.1.28
ExPASy - ENZYME nomenclature database: 1.4.1.28
BRENDA, the Enzyme Database: 1.4.1.28

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