KEGG   ENZYME: 1.4.3.26
Entry
EC 1.4.3.26                 Enzyme                                 

Name
pre-mycofactocin synthase;
mftD (gene name)
Class
Oxidoreductases;
Acting on the CH-NH2 group of donors;
With oxygen as acceptor
Sysname
3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one:oxygen oxidoreductase
Reaction(IUBMB)
3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one + O2 + H2O = 5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidine-2,3-dione + NH3 + H2O2 (overall reaction) [RN:R12684];
(1a) 3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one + O2 = 5-[(4-hydroxyphenyl)methyl]-3-imino-4,4-dimethylpyrrolidin-2-one + H2O2 [RN:R12685];
(1b) 5-[(4-hydroxyphenyl)methyl]-3-imino-4,4-dimethylpyrrolidin-2-one + H2O = 5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidine-2,3-dione + NH3 (spontaneous) [RN:R12686]
Reaction(KEGG)
Substrate
3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one [CPD:C22330];
O2 [CPD:C00007];
H2O [CPD:C00001];
5-[(4-hydroxyphenyl)methyl]-3-imino-4,4-dimethylpyrrolidin-2-one [CPD:C22332]
Product
5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidine-2,3-dione [CPD:C22331];
NH3 [CPD:C00014];
H2O2 [CPD:C00027];
5-[(4-hydroxyphenyl)methyl]-3-imino-4,4-dimethylpyrrolidin-2-one [CPD:C22332]
Comment
A flavoprotein (FMN). The enzyme participates in the biosynthesis of the enzyme cofactor mycofactocin. The enzyme uses oxygen as an electron source to oxidize a C-N bond, followed by spontaneous exchange with water to form an alpha-keto moiety on the resulting molecule.
History
EC 1.4.3.26 created 2020
Orthology
K24693  pre-mycofactocin synthase
Genes
MTU: Rv0694
MTV: RVBD_0694
MTC: MT0721
MRA: MRA_0702(lldD1)
MTF: TBFG_10708
MTB: TBMG_00707
MTK: TBSG_00711
MTZ: TBXG_000700
MTI: MRGA423_04330
MTE: CCDC5079_0645
MTUR: CFBS_0730(lldD1)
MTO: MTCTRI2_0710(lldD1)
MTD: UDA_0694(lldD1)
MTN: ERDMAN_0766(lldD1)
MTUE: J114_03705
MTUH: I917_04945
MTUL: TBHG_00689
MTUT: HKBT1_0730(lldD1)
MTUU: HKBT2_0731(lldD1)
MTQ: HKBS1_0730(lldD1)
MBO: BQ2027_MB0713(lldD1)
MBB: BCG_0743(lldD1)
MBT: JTY_0713(lldD1)
MBM: BCGMEX_0714(lldD1)
MBX: BCGT_0486
MAF: MAF_07030(lldD1)
MMIC: RN08_0774
MCE: MCAN_06951(lldD1)
MCQ: BN44_10764(lldD)
MCV: BN43_20126(lldD)
MCX: BN42_20449(lldD)
MCZ: BN45_10794(lldD)
MPA: MAP_4154(lldD1)
MAO: MAP4_4280
MAVI: RC58_21260
MAVU: RE97_21310
MAV: MAV_4477
MIT: OCO_43680
MIA: OCU_43440
MID: MIP_06628
MYO: OEM_43910
MIR: OCQ_44790
MLP: MLM_3633
MMAN: MMAN_31910
MUL: MUL_0774(lldD1)
MMC: Mmcs_1002
MKM: Mkms_1019
MJL: Mjls_1029
MMI: MMAR_1022(lldD1)
MMAE: MMARE11_09750(lldD1)
MMM: W7S_22020
MLI: MULP_01149(lldD1)
MHAD: B586_18790
MSHG: MSG_00883(mftD)
MFJ: MFLOJ_25790(mftD)
MSTO: MSTO_29430(lldD1)
MSIM: MSIM_16070(mftD)
MSAK: MSAS_44580(lldD1)
MGRO: FZ046_11355(mftD)
MXE: MYXE_39980(mftD)
MNV: MNVI_25250(mftD)
MGOR: H0P51_05080(mftD)
MCOO: MCOO_41770(mftD)
MBAI: MB901379_00883(lldD_1)
MSEO: MSEO_48260(mftD)
MPSE: MPSD_11590(lldD1)
MSHO: MSHO_28110(mftD)
MHEK: JMUB5695_03903(mftD)
MGAU: MGALJ_54740(mftD)
MLJ: MLAC_34940(mftD)
MBRD: MBRA_06480(lldD1)
MSHJ: MSHI_27510(lldD1)
MDF: K0O62_05975(mftD)
MMAM: K3U93_04270(mftD)
MSG: MSMEI_1389(lldD1)
MVA: Mvan_1297
MGI: Mflv_5059
MPHL: MPHLCCUG_01294(lldD)
MVQ: MYVA_1102
MTHN: 4412656_00904(lldD)
MHAS: MHAS_00223(mftD)
MAUU: NCTC10437_01080(lldD)
MMAG: MMAD_11160
MMOR: MMOR_54170(mftD)
MAIC: MAIC_45860
MALV: MALV_24320
MPSC: MPSYJ_14440(lldD1) MPSYJ_53840
MARZ: MARA_27870
MGAD: MGAD_28130(lldD1)
MHEV: MHEL_11870
MSAR: MSAR_25560
MAUB: MAUB_22110
MPOF: MPOR_01030(lldD1) MPOR_15790
MPHU: MPHO_48450
MMUC: C1S78_023000(mftD)
MBOK: MBOE_62320
MAB: MAB_3834c
MABB: MASS_3845
MCHE: BB28_19630
MSTE: MSTE_03983
MSAL: DSM43276_03627(lldD)
MJD: JDM601_3347(lldD1)
MTER: 4434518_03334(lldD1)
MMIN: MMIN_10490
MHIB: MHIB_27750
NFR: ERS450000_00862(lldD_1) ERS450000_01865(lldD_2)
NOZ: DMB37_06070(mftD)
NAH: F5544_26880(mftD)
NAD: NCTC11293_04501(lldD)
RER: RER_18000
REY: O5Y_08635
ROP: ROP_61310
REQ: REQ_36260
RHB: NY08_1951
RFA: A3L23_00296(mftD_2)
RHS: A3Q41_03118(mftD_1)
RHU: A3Q40_00413(mftD)
RRT: 4535765_03564(lldD)
RCR: NCTC10994_03688(mdlB)
RTM: G4H71_16290(mftD)
RKO: JWS14_33115(mftD)
RGO: KYT97_09555(mftD)
GBR: Gbro_3777
GOR: KTR9_3736
GIT: C6V83_16210(mftD)
GRU: GCWB2_19175(lldD)
GOM: D7316_04927(mftD)
GAV: C5O27_08965(mftD)
GOD: GKZ92_18105(mftD)
GAM: GII34_18045(mftD)
GPD: GII33_17500(mftD)
GJI: H1R19_17925(mftD)
SPIN: KV203_02220(mftD)
DIT: C3V38_01375(mftD)
TOY: FO059_05815(mftD)
SHY: SHJG_2442
SBRO: GQF42_07990(mftD)
MSED: E3O41_00715(mftD)
MCAW: F6J84_03085(mftD)
HUM: DVJ78_13580(mftD)
LTR: EVS81_11980(mftD)
HEA: HL652_05855(mftD)
CHRE: IE160_02970(mftD)
JLI: EXU32_11040(mftD)
NDP: E2C04_03205(mftD)
NAQU: ENKNEFLB_00510(mftD)
AEZ: C3E78_15425(mftD)
AEB: C6I20_03060(mftD)
AEF: GEV26_02915(mftD)
TFU: Tfu_2980
TCU: Tcur_2361
ACTW: F7P10_02080(mftD)
FAL: FRAAL3579
NML: Namu_0776
GOB: Gobs_0573
MMAR: MODMU_5387(lldD)
SEN: SACE_2390(lldD1)
SACG: FDZ84_25660(mftD) FDZ84_29570(mftD) FDZ84_34695(mftD)
AMQ: AMETH_2163(lldD1)
PDX: Psed_4052
PSEA: WY02_22925
PSEE: FRP1_19815
PSEH: XF36_04020
PAUT: Pdca_15250
PBRO: HOP40_01230(mftD)
ALO: CRK56115
ASE: ACPL_6088
ACTS: ACWT_5956
CATI: CS0771_62040(mftD)
EKE: EK0264_02670(mftD)
RXY: Rxyl_3151
AQZ: KSP35_11425(mftD)
IAM: HC251_02950(mftD)
TRO: trd_A0844
STI: Sthe_2860
 » show all
Reference
1  [PMID:31381312]
  Authors
Ayikpoe RS, Latham JA.
  Title
MftD Catalyzes the Formation of a Biologically Active Redox Center in the Biosynthesis of the Ribosomally Synthesized and Post-translationally Modified Redox Cofactor Mycofactocin.
  Journal
J Am Chem Soc 141:13582-13591 (2019)
DOI:10.1021/jacs.9b06102
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.4.3.26
IUBMB Enzyme Nomenclature: 1.4.3.26
ExPASy - ENZYME nomenclature database: 1.4.3.26
BRENDA, the Enzyme Database: 1.4.3.26

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