KEGG ENZYME: 1.4.3.26

ENZYME: 1.4.3.26

Entry

EC 1.4.3.26                 Enzyme

Name

pre-mycofactocin synthase;
mftD (gene name)

Class

Oxidoreductases;
Acting on the CH-NH2 group of donors;
With oxygen as acceptor

Sysname

3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one:oxygen oxidoreductase

Reaction(IUBMB)

3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one + O2 + H2O = 5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidine-2,3-dione + NH3 + H2O2 (overall reaction) [RN:R12684];
(1a) 3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one + O2 = 5-[(4-hydroxyphenyl)methyl]-3-imino-4,4-dimethylpyrrolidin-2-one + H2O2 [RN:R12685];
(1b) 5-[(4-hydroxyphenyl)methyl]-3-imino-4,4-dimethylpyrrolidin-2-one + H2O = 5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidine-2,3-dione + NH3 (spontaneous) [RN:R12686]

Reaction(KEGG)

R12684 R12685 R12686

Substrate

3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one [CPD:C22330];
O2 [CPD:C00007];
H2O [CPD:C00001];
5-[(4-hydroxyphenyl)methyl]-3-imino-4,4-dimethylpyrrolidin-2-one [CPD:C22332]

Product

5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidine-2,3-dione [CPD:C22331];
NH3 [CPD:C00014];
H2O2 [CPD:C00027];
5-[(4-hydroxyphenyl)methyl]-3-imino-4,4-dimethylpyrrolidin-2-one [CPD:C22332]

Comment

A flavoprotein (FMN). The enzyme participates in the biosynthesis of the enzyme cofactor mycofactocin. The enzyme uses oxygen as an electron source to oxidize a C-N bond, followed by spontaneous exchange with water to form an alpha-keto moiety on the resulting molecule.

History

EC 1.4.3.26 created 2020

Orthology

K24693

pre-mycofactocin synthase

Genes

MTU:

Rv0694

MTV:

RVBD_0694

MTC:

MT0721

MRA:	MRA_0702(lldD1)

MTF:	TBFG_10708

MTB:	TBMG_00707

MTK:	TBSG_00711

MTZ:	TBXG_000700

MTI:	MRGA423_04330

MTE:	CCDC5079_0645

MTUR:

CFBS_0730(lldD1)

MTL:	CCDC5180_0636

MTO:	MTCTRI2_0710(lldD1)

MTD:	UDA_0694(lldD1)

MTN:	ERDMAN_0766(lldD1)

MTJ:	J112_03715

MTUB:

MT7199_0712

MTUE:

J114_03705

MTX:	M943_03610

MTUH:

I917_04945

MTUL:

TBHG_00689

MTUT:

HKBT1_0730(lldD1)

MTUU:

HKBT2_0731(lldD1)

MTQ:	HKBS1_0730(lldD1)

MBO:	BQ2027_MB0713(lldD1)

MBB:	BCG_0743(lldD1)

MBT:	JTY_0713(lldD1)

MBM:	BCGMEX_0714(lldD1)

MBK:	K60_007400

MBX:

BCGT_0486

MAF:	MAF_07030(lldD1)

MMIC:

RN08_0774

MCE:	MCAN_06951(lldD1)

MCQ:	BN44_10764(lldD)

MCV:	BN43_20126(lldD)

MCX:	BN42_20449(lldD)

MCZ:	BN45_10794(lldD)

MORY:

MO_000739(mftD)

MPA:	MAP_4154(lldD1)

MAO:

MAP4_4280

MAVI:

RC58_21260

MAVU:

RE97_21310

MAV:

MAV_4477

MIT:

OCO_43680

MIA:

OCU_43440

MID:

MIP_06628

MYO:

OEM_43910

MCHI:

AN480_22765

MIR:

OCQ_44790

MMAL:

CKJ54_20790

MLP:

MLM_3633

MMAN:

MMAN_31910

MSER:

MTY59_07090(mftD)

MSA:	Mycsm_00994

MUL:	MUL_0774(lldD1)

MMI:	MMAR_1022(lldD1)

MMAE:

MMARE11_09750(lldD1)

MLI:	MULP_01149(lldD1)

MPSE:

MPSD_11590(lldD1)

MSHO:

MSHO_28110(mftD)

MMC:

Mmcs_1002

MKM:

Mkms_1019

MJL:

Mjls_1029

MMM:

W7S_22020

MKN:	MKAN_19595

MYV:	G155_07140

MYE:	AB431_05555

MHAD:

B586_18790

MDX:	BTO20_28585

MSHG:

MSG_00883(mftD)

MFJ:	MFLOJ_25790(mftD)

MSTO:

MSTO_29430(lldD1)

MSIM:

MSIM_16070(mftD)

MSAK:

MSAS_44580(lldD1)

MKU:	I2456_05205(mftD)

MLW:	MJO58_04705(mftD)

MXE:	MYXE_39980(mftD)

MNV:	MNVI_25250(mftD)

MPAG:

C0J29_05585

MNM:	MNVM_27260

MGOR:

H0P51_05080(mftD)

MCOO:

MCOO_41770(mftD)

MBAI:

MB901379_00883(lldD_1)

MSEO:

MSEO_48260(mftD)

MHEK:

JMUB5695_03903(mftD)

MGAU:

MGALJ_54740(mftD)

MLJ:	MLAC_34940(mftD)

MBRD:

MBRA_06480(lldD1)

MSHJ:

MSHI_27510(lldD1)

MMAM:

K3U93_04270(mftD)

MSPG:

F6B93_04320(mftD)

MOT:	LTS72_20555(mftD)

MPAA:

MKK62_01645(mftD) MKK62_20120(mftD)

MMEH:

M5I08_21205(mftD)

MKY:	IWGMT90018_10160

MADI:

A7U43_25305

MWU:	PT015_22330(mftD)

MYW:	BVC93_04255 BVC93_10175

MSM:	MSMEG_1424

MSG:	MSMEI_1389(lldD1)

MSB:	LJ00_07100

MSN:	LI99_07100

MSH:	LI98_07100

MVA:

Mvan_1297

MGI:

Mflv_5059

MSP:	Mspyr1_44770

MCB:	Mycch_0935 Mycch_5885

MNE:	D174_06610

MYN:	MyAD_06475

MFT:	XA26_14280

MPHL:

MPHLCCUG_01294(lldD)

MVQ:

MYVA_1102

MLL:	B1R94_05280 B1R94_22380

MRH:	MycrhN_0707 MycrhN_3065

MTHN:

4412656_00904(lldD)

MHAS:

MHAS_00223(mftD)

MDU:	MDUV_34200

MCHT:

MCHIJ_01190

MDR:	MDOR_06290

MAUU:

NCTC10437_01080(lldD)

MMAG:

MMAD_11160

MMOR:

MMOR_54170(mftD)

MFX:	MFAL_21350

MAIC:

MAIC_45860

MIJ:	MINS_13340

MALV:

MALV_24320

MTY:	MTOK_31930

MPSC:

MPSYJ_14440(lldD1) MPSYJ_53840

MARZ:

MARA_27870

MGAD:

MGAD_28130(lldD1)

MHEV:

MHEL_11870

MSAR:

MSAR_25560

MANY:

MANY_39930 MANY_49640 MANY_50610(mftD)

MAUB:

MAUB_22110

MPOF:

MPOR_01030(lldD1) MPOR_15790

MPHU:

MPHO_48450

MMUC:

C1S78_023000(mftD)

MMAT:

MMAGJ_31380(mftD) MMAGJ_42620

MBOK:

MBOE_62320

MFG:	K6L26_25500(mftD)

MSEI:

MSEDJ_23780

MFLV:

NCTC10271_04119(lldD)

MCEE:

MCEL_23180

MMON:

EWR22_05395(mftD)

MGO:	AFA91_31795

MHOL:

K3U96_21200(mftD)

MSEN:

K3U95_06220(mftD)

MPAE:

K0O64_05090(mftD)

MRF:	MJO55_05340(mftD) MJO55_24970(mftD)

MDF:	K0O62_05975(mftD)

MCRO:

MI149_05815(mftD)

MGRO:

FZ046_11355(mftD)

MAUS:

JN090_05240(mftD) JN090_27350(mftD)

MKR:	MKOR_22770

MPAK:

MIU77_15070(mftD)

MAB:

MAB_3834c

MMV:	MYCMA_2120

MABB:

MASS_3845

MABL:

MMASJCM_3849

MCHE:

BB28_19630

MIZ:	BAB75_21905

MSTE:

MSTE_03983

MSAO:

MYCSP_17700

MSAL:

DSM43276_03627(lldD)

MJD:	JDM601_3347(lldD1)

MTER:

4434518_03334(lldD1)

MMIN:

MMIN_10490

MHIB:

MHIB_27750

MHER:

K3U94_19115(mftD)

MVM:	MJO54_19080(mftD)

ASD:	AS9A_4329 AS9A_4369

NFA:	NFA_22320 NFA_32080

NFR:	ERS450000_00862(lldD_1) ERS450000_01865(lldD_2)

NCY:	NOCYR_3384

NNO:	NONO_c43720

NOZ:	DMB37_06070(mftD)

NAH:	F5544_26880(mftD)

NAD:	NCTC11293_04501(lldD)

NWL:	NWFMUON74_31330

NGP:	LTT66_26875(mftD)

NSPU:

IFM12276_22320(lldD1)

RHA:	RHA1_ro06073

RER:

RER_18000

REY:

O5Y_08635

REB:	XU06_08875

RQI:	C1M55_09110

ROP:

ROP_61310

ROA:	Pd630_LPD02714

RPY:	Y013_08770

RBY:	CEJ39_19665

RHB:

NY08_1951

RAV:	AAT18_18585

RHW:	BFN03_03200

RRZ:	CS378_08055

RHU:	A3Q40_00413(mftD)

RHQ:	IM25_00600

RHOD:

AOT96_19475

RRT:	4535765_03564(lldD)

RCR:	NCTC10994_03688(mdlB)

RTM:	G4H71_16290(mftD)

RKO:	JWS14_33115(mftD)

RGO:	KYT97_09555(mftD)

ROZ:	CBI38_20390

RPSK:

JWS13_10845(mftD)

RGOR:

NMQ04_15525(mftD)

RANT:

RHODO2019_14315(mftD) RHODO2019_14320(mftD)

RHOP:

D8W71_12600(mftD) D8W71_12605(mftD)

RHOO:

RA302_09020(mftD)

RHOU:

KZJ41_19685(mftD)

RHOW:

A4U64_06135

RFA:	A3L23_00296(mftD_2)

RHS:	A3Q41_03118(mftD_1)

REQ:

REQ_36260

PDEF:

P9209_16160(mftD)

WHR:	OG579_07505(mftD)

GBR:

Gbro_3777

GPO:	GPOL_c36470

GOR:

KTR9_3736

GOQ:	ACH46_15910

GTA:	BCM27_19565

GOC:	CXX93_06960

GIT:	C6V83_16210(mftD)

GRU:	GCWB2_19175(lldD)

GOM:	D7316_04927(mftD)

GAV:	C5O27_08965(mftD)

GOD:	GKZ92_18105(mftD)

GOS:	QAD21_18475(mftD)

GOJ:	GR168_17865(mftD)

GAM:	GII34_18045(mftD)

GPD:	GII33_17500(mftD)

GJI:	H1R19_17925(mftD)

GOI:	LK459_15810(mftD)

GHN:	MVF96_18490(mftD)

GAMI:

IHQ52_22080(mftD)

GMG:	NWF22_03540(mftD)

GSI:	P5P27_15280(mftD)

SPIN:

KV203_02220(mftD)

TSM:	ASU32_04095

TSD:	MTP03_02830

DTM:	BJL86_2335

DIT:	C3V38_01375(mftD)

DIZ:	CT688_11590

DPC:	A6048_12170

DLU:	A6035_11190

DKN:	NHB83_11610(mftD)

TOY:	FO059_05815(mftD)

SHY:

SHJG_2442

SHO:	SHJGH_2207

SBRO:

GQF42_07990(mftD)

STUI:

GCM10017668_53910

SPRA:

CP972_11020(mftD)

SCIR:

STRCI_007930(mftD)

SANT:

QR300_11385(mftD)

KBU:	Q4V64_47340(mftD)

YIA:	LO772_03295(mftD)

MSED:

E3O41_00715(mftD)

MCAW:

F6J84_03085(mftD)

MELY:

L2X98_23820(mftD)

FSB:	GCM10025867_43720

AARC:

G127AT_05870(mftD)

AMAV:

GCM10025877_11550(mftD)

CPHY:

B5808_07305

CHRE:

IE160_02970(mftD)

HUM:	DVJ78_13580(mftD)

LTR:	EVS81_11980(mftD)

LALL:

MUN78_01460(mftD)

LRP:	MUN76_06410(mftD)

HEA:	HL652_05855(mftD)

HEH:	L3i23_13060(lldD1)

SUBA:

LQ955_15000(mftD)

AJG:	KKR91_08560(mftD)

AZX:	N2K95_07770(mftD)

AJR:	N2K98_08380(mftD)

BEI:	GCM100_01110

JLI:	EXU32_11040(mftD)

JAY:	H7A72_14565(mftD)

NDP:	E2C04_03205(mftD)

NARO:

CFH99_21860 CFH99_24465

NAQU:

ENKNEFLB_00510(mftD)

NRO:	K8W59_04735(mftD)

NPC:	KUV85_09745(mftD)

NPS:	KRR39_16375(mftD)

NMAR:

HPC71_02605

NBI:	SHK19_15705(mftD)

PSIM:

KR76_00720 KR76_24780

AEZ:	C3E78_15425(mftD)

AEB:	C6I20_03060(mftD)

AEF:	GEV26_02915(mftD)

NBT:	KLP28_13000(mftD)

TFU:

Tfu_2980

THAO:

NI17_018885(mftD)

TALX:

FOF52_16185(mftD)

SAIU:

J4H86_09355(mftD)

TCU:

Tcur_2361

ACTW:

F7P10_02080(mftD)

AMAZ:

LUW76_43945(mftD)

FAL:

FRAAL3579

FRE:	Franean1_3205

NML:

Namu_0776

NAKA:

H7F38_19405(mftD)

GOB:

Gobs_0573

BSD:	BLASA_4816

BBRG:

U6N30_28165(mftD)

MMAR:

MODMU_5387(lldD)

SEN:	SACE_2390(lldD1)

SACG:

FDZ84_25660(mftD) FDZ84_29570(mftD) FDZ84_34695(mftD)

AMQ:	AMETH_2163(lldD1)

AMYC:

CU254_14340

APRT:

MUY14_18335(mftD)

AROO:

NQK81_28205(mftD)

ATHM:

L1857_13410(mftD)

ARHD:

VSH64_10225(mftD)

AMOG:

QRX60_23460(mftD) QRX60_42450(mftD)

ACYN:

ORV05_24515(mftD)

PDX:

Psed_4052

PSEA:

WY02_22925

PSEE:

FRP1_19815

PSEH:

XF36_04020

PSEQ:

AD006_27230

PECQ:

AD017_06825

PHH:	AFB00_18590

PAUT:

Pdca_15250

PBRO:

HOP40_01230(mftD)

PPEL:

H6H00_24630(mftD)

ALO:

CRK56115

PMAD:

BAY61_16740

ACTY:

OG774_18855(mftD)

ASE:

ACPL_6088

AMS:	AMIS_27210

ACTS:

ACWT_5956

ACTL:

L3i22_048290 L3i22_048330(mftD)

CATI:

CS0771_62040(mftD)

DROS:

Drose_15385(mftD)

DACT:

KZZ52_13945(mftD)

EKE:	EK0264_02670(mftD)

RXY:

Rxyl_3151

RRD:	RradSPS_2637

RMAR:

GBA65_20425(mftD)

SBAE:

DSM104329_01720(mftD)

AYM:	YM304_36360

AQZ:	KSP35_11425(mftD)

IAM:	HC251_02950(mftD)

TRO:

trd_A0844

STI:

Sthe_2860

MICA:

P0L94_00220(mftD)

» show all

Reference

1 [PMID:31381312]

Authors

Ayikpoe RS, Latham JA.

Title

MftD Catalyzes the Formation of a Biologically Active Redox Center in the Biosynthesis of the Ribosomally Synthesized and Post-translationally Modified Redox Cofactor Mycofactocin.

Journal

J Am Chem Soc 141:13582-13591 (2019)
DOI:10.1021/jacs.9b06102

Sequence

[msm:MSMEG_1424]

Other DBs

ExplorEnz - The Enzyme Database:

1.4.3.26

IUBMB Enzyme Nomenclature:

1.4.3.26

ExPASy - ENZYME nomenclature database:

1.4.3.26

BRENDA, the Enzyme Database:

1.4.3.26

DBGET integrated database retrieval system