KEGG   ENZYME: 1.4.7.1
Entry
EC 1.4.7.1                  Enzyme                                 
Name
glutamate synthase (ferredoxin);
ferredoxin-dependent glutamate synthase;
ferredoxin-glutamate synthase;
glutamate synthase (ferredoxin-dependent)
Class
Oxidoreductases;
Acting on the CH-NH2 group of donors;
With an iron-sulfur protein as acceptor
Sysname
L-glutamate:ferredoxin oxidoreductase (transaminating)
Reaction(IUBMB)
2 L-glutamate + 2 oxidized ferredoxin = L-glutamine + 2-oxoglutarate + 2 reduced ferredoxin + 2 H+ (overall reaction) [RN:R00021];
(1a) L-glutamate + NH3 = L-glutamine + H2O [RN:R00256];
(1b) L-glutamate + 2 oxidized ferredoxin + H2O = NH3 + 2-oxoglutarate + 2 reduced ferredoxin + 2 H+ [RN:R10086]
Reaction(KEGG)
Substrate
L-glutamate [CPD:C00025];
oxidized ferredoxin [CPD:C00139];
NH3 [CPD:C00014];
H2O [CPD:C00001]
Product
L-glutamine [CPD:C00064];
2-oxoglutarate [CPD:C00026];
reduced ferredoxin [CPD:C00138];
H+ [CPD:C00080];
H2O [CPD:C00001];
NH3 [CPD:C00014]
Comment
Binds a [3Fe-4S] cluster as well as FAD and FMN. The protein is composed of two domains, one hydrolysing L-glutamine to NH3 and L-glutamate (cf. EC 3.5.1.2, glutaminase), the other combining the produced NH3 with 2-oxoglutarate to produce a second molecule of L-glutamate. The NH3 is channeled through a 24 A channel in the active protein. No hydrolysis of glutamine takes place without ferredoxin and 2-oxoglutarate being bound to the protein [5,6].
History
EC 1.4.7.1 created 1976, modified 2012
Pathway
ec00630  Glyoxylate and dicarboxylate metabolism
ec00910  Nitrogen metabolism
ec01120  Microbial metabolism in diverse environments
Orthology
K00284  glutamate synthase (ferredoxin)
Genes
ATHAT2G41220(GLU2) AT5G04140(GLU1)
ALY9309205 9315983
CRB17882431 17889161
CSAT104708708 104734430 104768604 104782609 104785266 104793031
EUSEUTSA_v10012425mg EUTSA_v10016134mg
BRP103847326 103857972 103865867
BNA106352787 106372522 106372673 106391644 106431110
BOE106317861 106335201 106342177
RSZ108817709 108854286
THJ104803262 104816414
CPAP110822870
CIT102609089
CICCICLE_v10004137mg
PVY116132388
MINC123197667 123205484
TCC18598741
GRA105786202
GHI107921512 107921972
GAB108466881
DZI111293883 111311455
EGR104444178
GMX100809299 548016
GSJ114400728 114406643
PVUPHAVU_001G123900g
VRA106752554
VAR108328874
VUN114186821
CCAJ109815625
APRC113863103
MTR11415038
CAM101506279
PSAT127126429
LJALj1g3v4154900.1(Lj1g3v4154900.1) Lj1g3v4154900.2(Lj1g3v4154900.2) Lj1g3v4154900.3(Lj1g3v4154900.3)
ADU107492689
AIP107645553
AHF112695943 112756317
LANG109343289
FVE101314871
RCN112188077
PPER18770391
PMUM103341391
PAVI110758098
PDUL117633614
MDM114820893
PXB103928813
ZJU107433784 107435873
MNT21402329
CSV101214380
CMO103486888
BHJ120090125
MCHA111016634
CMAX111469981 111482225
CMOS111441624 111446589
CPEP111789977 111800589
RCU8270489
HBR110658224 110664043
MESC110622961
POP7458925 7495696
PEU105108000 105127577
PALZ118033263 118053033
JRE108995228 109011587
QSU112004190
QLO115952760
TWL119992974 119998497
VVI100233082
VRI117920766
SLY101246028
SPEN107013679
SOT102600112
SSTN125857428
CANN107852264
NTA107781744 107793691
NSY104241431
NTO104118686
NAU109233296
INI109161932
ITR116024931
SIND105159899
OEU111365860 111379166
EGT105949319
SSPL121795818 121799482
HAN110928568
ECAD122581218
LSV111891706
CCAV112512315
DCR108226274
CSIN114293314
BVG104889481
SOE110777056
CQI110710572
NNU104611350
MING122057294
TSS122668165
PSOM113309085 113320934
NCOL116246228
OSA4344164
DOSAOs07t0658400-01(Os07g0658400)
OBR102718939
BDI100827937
ATS109733233
TDC119353479 119362636
TAES123043871 123051744 123187649
SBI8073135
ZMA542710
SITA101766581
SVS117843736
PVIR120662072 120694741
PHAI112879687
PDA103701654 103716926
EGU105049124 105057601
MUS103976157
DCT110101016
PEQ110034398
AOF109849057
ATR18444596
SMOSELMODRAFT_155359 SELMODRAFT_161275 SELMODRAFT_448225
PPP112284730 112286024
CRECHLRE_12g514050v5
VCNVOLCADRAFT_127156(glu1)
MNGMNEG_13125
CSLCOCSUDRAFT_22625
CVRCHLNCDRAFT_142154
APROF751_5489
OLUOSTLU_51965
OTAOT_ostta14g01900
BPGBathy12g01040
MISMICPUN_63658(GLU)
MPPMICPUCDRAFT_70244(GLU)
CMECymeCp051(gltB)
GSLGasu_40050 JL72_p025(gltB)
CCPCHC_1080(gltB)
PTIPHATRDRAFT_56605
FCYFRACYDRAFT_225787
TPSTHAPSDRAFT_269900(glsF)
AAFAURANDRAFT_37118(FG)
EHXEMIHUDRAFT_225692
GTTGUITHDRAFT_88543
EBSECTOBSL9_2331
GERKP004_06430(gltB)
GSUBKP001_08935(gltB)
GNTKP003_05915(gltB)
GPLM1B72_14475(gltB)
AORYAMOR_34870(gltB)
APAUAMPC_36480(gltB)
PCAYFRD00_24080(gltB)
BBAEFRD01_18100(gltB)
MSEMGMB29_09955(gltB)
CTHUHUR95_04910(gltB)
SLMSMM221_01525(gltB)
CCHLFPL14_09895(gltB)
CHEBHH215_17895(gltB)
RGVNQ502_17400(gltB)
SVJNQ490_13625(gltB)
LASAL9O85_08735(gltB)
BCRONQ527_04160(gltB)
BLIQINP51_06770(gltB)
BWXNQ550_06850(gltB)
CASPNQ535_15715(gltB)
SINTK5I26_15105(gltB)
WCPH9Q76_10615(gltB)
QDOH9Q78_13590(gltB)
WHJH9Q79_04290(gltB)
MFORNQ534_15145(gltB)
TMRTmar_0631
THEPDYI95_002945
VRGOKW85_08730(gltB)
AMUOKWG62_00095(gltB)
ATQGH723_08305
EUZDVS28_a2831
SYNsll1499(gltB) sll1502(gltB)
SYZMYO_130230(gltB) MYO_14470(gltB)
SYYSYNGTS_0442(gltB) SYNGTS_2989(gltB)
SYTSYNGTI_0442(gltB) SYNGTI_2988(gltB)
SYSSYNPCCN_0442(gltB) SYNPCCN_2987(gltB)
SYQSYNPCCP_0442(gltB) SYNPCCP_2987(gltB)
SYJD082_27210(glsF) D082_30770(gltB)
SYOC7I86_02300 C7I86_15650
SYCsyc0650_c(glsF)
SYUM744_12285
SYFSynpcc7942_0890
SYDSyncc9605_0326
SYESyncc9902_2016
SYGsync_0387
SYRSynRCC307_2164(gltS)
SYXSynWH7803_0385(gltS)
SYPSYNPCC7002_A2393(gltS)
CYACYA_2704(gltS)
CYBCYB_1253(gltS)
SYNESyn6312_2924
SYNPSyn7502_00626
SYNKKR100_03040
SYNRKR49_10635
SYNDKR52_00805
SYHSyncc8109_0331(glsF)
SYNWSynWH8103_02451(glsF)
SYVAWQ23_11920
SYLAWQ21_12565
SYNCCB0101_10210(gltB)
SYNYBM449_08435
DSLDacsa_3392
SYWSYNW2132(glsF)
CGCCyagr_1859
CYICBM981_1898(gltS)
PMAPro_1668(glsF)
PMMPMM1512(glsF)
PMTPMT_1777
PMNPMN2A_1078
PMIPMT9312_1604
PMBA9601_17161(gltB)
PMCP9515_16921(gltB)
PMFP9303_23581(gltB)
PMGP9301_17041(gltB)
PMHP9215_17801(gltB)
PMJP9211_16351(gltB)
PMENATL1_19531(gltB)
PRMEW15_2017
CMPCha6605_3414 Cha6605_4807
AMRAM1_1662(gltB) AM1_6366
WNAKA717_33855 KA717_38915(gltB)
TVNNIES2134_111810(glsF)
SLWBRW62_01870
TELtll1368(glsF)
THNNK55_02305(glsF)
THECFFX45_09225
LEPLepto7376_1007
LENLEP3755_14620 LEP3755_25630
LETO77CONTIG1_00377(gltS_2) O77CONTIG1_04635(gltB_2)
LBOLBWT_33050 LBWT_36910
KOVK9N68_24780(gltB) K9N68_26565
LSCKIK02_11125(gltB) KIK02_23740(gltB)
LSKJ5X98_13485(gltB) J5X98_15655(gltB)
HHGXM38_009140(gltS)
PSEUPse7367_2837
PSERABRG53_0383(gltB) ABRG53_2376(gltS)
THEUHPC62_07970(gltB) HPC62_18475(gltB)
GLPGlo7428_3738
GENGM3709_3385 GM3709_950
GEEGM3708_2694 GM3708_514
CHONNIES4102_36890(glsF)
MARMAE_07560(gltB) MAE_29110(glsF)
MPKVL20_163 VL20_5286
MIQB5D77_15080 B5D77_21585
MVZmyaer102_05130(glsF) myaer102_42050(gltB)
CANCyan10605_0489
CSNCyast_0608 Cyast_0917
CYLAA637_10340(gltB) AA637_11000
HAOPCC7418_1264 PCC7418_1589
ENNFRE64_12340(gltB)
CYUUCYN_03690
CYTcce_4353(glsF)
CWACwatDRAFT_5417
CYPPCC8801_3730
CYHCyan8802_3782
CYCPCC7424_1468 PCC7424_3654
CYJCyan7822_2992 Cyan7822_4149
CYNCyan7425_0526
TERTery_0466
ARPNIES39_J05540(glsF)
PAGHNIES204_16940 NIES204_23340(glsF)
PPSUNO713_01836(gltS) NO713_05437(gltB)
OXYHCG48_19975
MVAGD0A34_12415 D0A34_16000
LFSHFV01_04520(gltB)
MICMic7113_1425 Mic7113_6228
GEIGEI7407_2827
OACOscil6304_3505 Oscil6304_4177
ONIOsc7112_1580 Osc7112_2538
MPROBJP34_31320
PHORJWS08_08250(gltB) JWS08_11275(gltB)
PYHNEA10_01415(gltB)
CEPCri9333_0783 Cri9333_3015
GVIglr1508(glsF)
GLJGKIL_0118(gltS)
GMLISF26_17045
ANAalr4344(gltS)
NPUNpun_R3877
NOSNos7107_2676
NOPNos7524_4221
NONNOS3756_08790
NFLCOO91_04534
NOECLI64_22005
NSHGXM_03059
NEDHUN01_14830
AVAAva_1294
NAZAazo_0653
ANBANA_C10135(gltS)
ACYAnacy_2495
AWAAA650_06545
ANNEH233_11960
CSGCylst_5006
RSINB6N60_00992
CALOCal7507_2925
CALTCal6303_1504
CALHIJ00_19245
CALNNIES2098_46520
RIVRiv7116_1068
FISFIS3754_01900
NSPHBDGGKGIB_02156(gltS)
DOUBMF77_02710(gltS)
DFSHGD76_03835(gltB)
DHTNG743_20300
CCURIAR63_15135
CRKL3I90_16415
AFLOHEQ12_18625
TOQHCG51_11690(gltB)
NCNBZZ01_24715
CTHEChro_3057 Chro_3459
PLPPle7327_4235 Ple7327_4643
SCSSta7437_2250 Sta7437_2290
STANSTA3757_00340 STA3757_02320(gltB)
CEOETSB_0993(glsF)
CERRGRSB_0946(glsF)
RRSRoseRS_2985
RCARcas_1903
CAUCaur_3258
CHLChy400_3517
CAGCagg_0145
HAUHaur_4476
TROtrd_1105
LEKhrd7_07070(gltB)
CAPCLDAP_26600(gltB)
KBSEPA93_24905(gltB)
THUGKNN16_03195(gltB)
TRATrad_0209
OPROcepr_1168
MHDMarky_1283
FGIOP10G_3032
VBHCMV30_08635
OLEK0B96_16910(gltB)
OBTOPIT5_11435
RUFIK0V07_15545(gltB)
ROOG5S37_25000(gltB)
LAMBKBB96_08710(gltB)
SOAG3M56_005025(gltB)
MEAPMTHMO_1987(gltS)
VBAIMCC26134_00205
GESVT84_11790(gltS)
GMSSOIL9_14000
TIMGMBLW1_42690
FTJFTUN_8218
ULIETAA1_22910(gltB)
TSPHKIH39_16360
MCADPan265_27950(gltB_2)
TAERGT409_07855(gltB)
ACAACP_2997(gltB)
ACMAciX9_1897
GMAAciX8_2097
GRWFTO74_06975
GRBGOB94_02530
TSAAciPR4_2050
TRSTerro_1714
TALBFTW19_14165
EDAGWR55_04770
EDGH7846_10995
ADINH7849_08145(gltB)
CVLJ8C06_04680(gltB)
CHLOJ8C02_06370(gltB)
SCORJ3U87_09960(gltB)
GAUGAU_3081(gltB)
GPHGEMMAAP_15705
GGRHKW67_20475(gltB)
SRMSRM_01351(gltB)
FPBNLJ00_11715(gltB)
PUNNQP51_08365(gltB)
LPALLDL79_15180(gltB)
FLBLV704_12170(gltB)
SAHNJRG66_08800(gltB)
CTSCtha_0711
HDLHALDL1_06165
HHBHhub_1568(gltB)
HNOLT974_02880(gltB)
HLULT972_10785(gltB)
HJEHacjB3_13680
HSUHLASF_0085(gltA1)
HSFHLASA_0085(gltA1)
SALRFQU85_01415(gltB)
HALREFA46_006790(gltB)
HAADMW046_08420(gltB)
HALXM0R89_12695(gltB)
HAXZM0R88_13050(gltB)
SALIL593_09320
SAIMK0C01_03750(gltB)
HARAAArcS_2470(gltB)
HMArrnAC0169(gltB)
HHIHAH_0919(gltB)
HHNHISP_04735
HABSG26_12425
HTABVU17_04490
HALJG9465_07175(gltB) G9465_07910(gltB)
HSINKDQ40_08925(gltB)
NPHNP_1794A(gltB)
NMONmlp_2987(gltB)
NHOHWV23_13415(gltB)
NSALHWV07_05345(gltB)
HALAHrd1104_06280
HMUHmuk_3135
HALZE5139_02915(gltB)
HALLLC1Hm_2478(gltS)
HAZPGBQ70_02915(gltB)
HALIBV210_03985
HSNDV733_03050 DV733_16480
HDSHSR122_1784(gltB) HSR122_2266(gltB3)
HRRHZS55_11310(gltB)
HPELHZS54_09415(gltB)
HLTI7X12_01920(gltB)
HDOMUK72_07660(gltB)
SSAIN0B31_00390(gltB)
HWAHQ_1714A(gltB)
HWCHqrw_1833(gltB)
HVOHVO_0869(gltB)
HMEHFX_0844(gltS)
HGIABY42_04180
HALEG3A49_04365(gltB)
HLSKU306_08970(gltB)
HBOHbor_23510
HAQDU484_08370
HAJDU500_08410
HAERDU502_05770
HRAEI982_08020
HLMDV707_13265
HALMFCF25_11350(gltB)
HLAHlac_0912
HALPDOS48_03595
HALBEKH57_07395
HEZZEO776_04990
HALQHrr1229_002420(gltB)
HSSJ7656_11845(gltB)
HSAIHPS36_02170(gltB)
HAZZKI388_02545(gltB)
SRUBC2R22_04850
HAEhalTADL_0125
HALNB4589_007465(gltB)
HALGHUG10_00530(gltB)
HALUHUG12_17205(gltB)
HMPK6T50_14680(gltB)
HREK6T36_14635(gltB)
HRMK6T25_02120(gltB)
HACBHbl1158_06740(gltB)
HDFAArcSl_1360(gltB)
HTUHtur_3198
HDABB347_10485
HSALJMJ58_12465(gltB)
HLOJ0X27_05560(gltB)
HAKZJ0X25_03350(gltB)
NMGNmag_2529(gltB)
HXAHalxa_3804
NATNJ7G_2607
NPENatpe_0471
NVRFEJ81_02705(gltB)
NPLFGF80_08195(gltB)
NAYHYG81_08095(gltB)
HALYHYG82_11285(gltB)
HJTDVR14_11645
NGENatgr_3664
NOUNatoc_2112
HLRHALLA_17405
HLCCHINAEXTREME00065
NAJB1756_00660
NAGAArcMg_1525
NANAArc1_2117
NBGDV706_09260
NASGCU68_02815(gltB)
SAWLNGM29_09860(gltB)
 » show all
Reference
1  [PMID:24254054]
  Authors
Galvan F, Marquez AJ, Vega JM
  Title
Purification and molecular properties of ferredoxin-glutamate synthase from Chlamydomonas reinhardii.
  Journal
Planta 162:180-7 (1984)
DOI:10.1007/BF00410216
Reference
2  [PMID:4423889]
  Authors
Lea PJ, Miflin BJ.
  Title
Alternative route for nitrogen assimilation in higher plants.
  Journal
Nature 251:614-6 (1974)
DOI:10.1038/251614a0
Reference
3  [PMID:12069605]
  Authors
Ravasio S, Dossena L, Martin-Figueroa E, Florencio FJ, Mattevi A, Morandi P, Curti B, Vanoni MA
  Title
Properties of the recombinant ferredoxin-dependent glutamate synthase of Synechocystis PCC6803. Comparison with the Azospirillum brasilense NADPH-dependent enzyme and its isolated alpha subunit.
  Journal
Biochemistry 41:8120-33 (2002)
DOI:10.1021/bi020083r
Reference
4  [PMID:10898944]
  Authors
Navarro F, Martin-Figueroa E, Candau P, Florencio FJ
  Title
Ferredoxin-dependent iron-sulfur flavoprotein glutamate synthase (GlsF) from the  Cyanobacterium synechocystis sp. PCC 6803: expression and assembly in Escherichia coli.
  Journal
Arch Biochem Biophys 379:267-76 (2000)
DOI:10.1006/abbi.2000.1894
Reference
5  [PMID:11967268]
  Authors
van den Heuvel RH, Ferrari D, Bossi RT, Ravasio S, Curti B, Vanoni MA, Florencio FJ, Mattevi A
  Title
Structural studies on the synchronization of catalytic centers in glutamate synthase.
  Journal
J Biol Chem 277:24579-83 (2002)
DOI:10.1074/jbc.M202541200
Reference
6  [PMID:12818206]
  Authors
van den Heuvel RH, Svergun DI, Petoukhov MV, Coda A, Curti B, Ravasio S, Vanoni MA, Mattevi A
  Title
The active conformation of glutamate synthase and its binding to ferredoxin.
  Journal
J Mol Biol 330:113-28 (2003)
DOI:10.1016/S0022-2836(03)00522-9
Other DBs
ExplorEnz - The Enzyme Database: 1.4.7.1
IUBMB Enzyme Nomenclature: 1.4.7.1
ExPASy - ENZYME nomenclature database: 1.4.7.1
BRENDA, the Enzyme Database: 1.4.7.1
CAS: 62213-56-3

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