KEGG ENZYME: 1.4.9.1

ENZYME: 1.4.9.1

Entry

EC 1.4.9.1                  Enzyme

Name

methylamine dehydrogenase (amicyanin);
amine dehydrogenase;
primary-amine dehydrogenase;
amine: (acceptor) oxidoreductase (deaminating);
primary-amine:(acceptor) oxidoreductase (deaminating)

Class

Oxidoreductases;
Acting on the CH-NH2 group of donors;
With a copper protein as acceptor

Sysname

methylamine:amicyanin oxidoreductase (deaminating)

Reaction(IUBMB)

methylamine + H2O + 2 amicyanin = formaldehyde + NH3 + 2 reduced amicyanin [RN:R00606]

Reaction(KEGG)

R00606

Substrate

methylamine [CPD:C00218];
H2O [CPD:C00001];
amicyanin [CPD:C19671]

Product

formaldehyde [CPD:C00067];
NH3 [CPD:C00014];
reduced amicyanin [CPD:C19672]

Comment

Contains tryptophan tryptophylquinone (TTQ) cofactor. The enzyme oxidizes aliphatic monoamines and diamines, histamine and ethanolamine, but not secondary and tertiary amines, quaternary ammonium salts or aromatic amines.

History

EC 1.4.9.1 created 1978 as EC 1.4.99.3, modified 1986, transferred 2011 to EC 1.4.98.1, transferred 2011 to EC 1.4.9.1

Pathway

ec00680

Methane metabolism

ec01100

Metabolic pathways

ec01120

Microbial metabolism in diverse environments

Orthology

K08685

quinohemoprotein amine dehydrogenase

K15228

methylamine dehydrogenase light chain

K15229

methylamine dehydrogenase heavy chain

Genes

YMO:	HRD69_07500 HRD69_07515

CARU:

P0E69_20625

VFU:	vfu_A02015 vfu_A02018

VFL:	AL536_13995 AL536_14010

PGH:	FH974_17590 FH974_17605

PRE:	PCA10_23420(mauB) PCA10_23450(mauA) PCA10_32510(peaA) PCA10_34250(mauB) PCA10_34280(mauA)

PFUW:

KF707C_23300 KF707C_23330 KF707C_25120

POJ:	PtoMrB4_25230

PLAL:

FXN65_11130 FXN65_11145 FXN65_11785(peaA)

PCQ:	PcP3B5_29870 PcP3B5_30140(aauA) PcP3B5_30170(aauB)

PMUI:

G4G71_11895(peaA) G4G71_16135 G4G71_16150

PNT:	G5B91_13190(peaA) G5B91_15900 G5B91_15915 G5B91_33160(peaA)

PPU:	PP_5602(peaA)

PPF:

Pput_2307

PPG:	PputGB1_2221 PputGB1_2224 PputGB1_2475

PPW:	PputW619_2945

PPT:

PPS_2940

PPB:	PPUBIRD1_2330

PPI:

YSA_11298

PPX:

T1E_1613

PPUH:

B479_14560

PPUT:

L483_18160

PPUN:

PP4_23960(peaA)

PPUD:

DW66_3200

PMON:

X969_14050

PMOT:

X970_13695

PPJ:	RK21_01725

PMOL:

CLJ08_00110(peaA)

PALD:

LU682_012715(peaA)

PIX:	RIN61_26575(peaA)

PMOS:

O165_011585

PFL:	PFL_4120(peaA)

PPRC:

PFLCHA0_c41800

PPRO:

PPC_4221(peaA)

PFO:	Pfl01_2975

PMAN:

OU5_1439

PEN:	PSEEN2552 PSEEN2555 PSEEN2977

PDR:	H681_11585

PSV:	PVLB_12820

PKC:	PKB_2656(mauA) PKB_2659(mauB) PKB_2912

PCH:	EY04_20885

PCZ:	PCL1606_20620

PCP:	JM49_10330

PCHP:

C4K32_4281

PALK:

PSAKL28_24530

PSEM:

TO66_21570

PSEC:

CCOS191_2324

PSOS:

POS17_4220(peaA)

PFK:	PFAS1_02675

PSET:

THL1_3122 THL1_3125 THL1_3144 THL1_3147 THL1_3176

PSIL:

PMA3_20245

PYM:	AK972_2150 AK972_2153

PKE:	DLD99_19475(peaA)

PUM:	HGP31_18355 HGP31_18370 HGP31_22220(peaA)

PMAO:

PMYSY11_1047 PMYSY11_1050(mauA)

PSEP:

C4K39_3038 C4K39_3041

PPII:

QL104_15105 QL104_15120

PVW:	HU752_028865(peaA)

PQI:	KH389_12680(peaA)

PXN:	HU772_009655 HU772_009670 HU772_012685(peaA)

PANH:

HU763_013035(peaA)

PASG:

KSS96_11100 KSS96_11115

PMAM:

KSS90_11360(peaA)

PAZE:

KSS91_08660(peaA)

PORY:

EJA05_14485(peaA)

PTW:	TUM18999_24310 TUM18999_32970 TUM18999_33000

PWY:	HU734_013230(peaA)

PCAV:

D3880_11325(peaA)

PIZ:	LAB08_R20860 LAB08_R20890 LAB08_R42330(peaA)

PSIH:

LOY51_14115(peaA)

PFIT:

KJY40_20070(peaA)

PASI:

LG197_20270(peaA)

PKM:	PZ739_15150(peaA) PZ739_16400 PZ739_16415

PNB:	NK667_05315(peaA)

PSOA:

PSm6_45540 PSm6_50130 PSm6_50160

PTAI:

ICN73_18625(peaA)

PKK:	QQ992_12005(peaA)

PKJ:	Q1W70_18750(peaA) Q1W70_25785 Q1W70_25800

PHOM:

KJF94_17245(peaA)

PTRL:

OU419_12830(peaA) OU419_13225 OU419_13240

PSJY:

AA098_12455

PSHS:

JJN09_10915(peaA)

PSNC:

C0058_12620 C0058_12635

PFOU:

OZ911_15435(peaA)

PBEJ:

PSH84_16300(peaA)

PSKP:

KU43P_23980 KU43P_30770 KU43P_30800

PDEU:

QEP73_09025(peaA)

PSDT:

NH234_15940 NH234_15955 NH234_20610(peaA)

PSWO:

SD196_15255 SD196_15270 SD196_15935(peaA)

PMK:	MDS_4213 MDS_4216

PKH:	JLK41_14070(peaA)

PBB:	AKN87_05350

PDEN:

F1C79_04845(peaA) F1C79_07630 F1C79_07645

HARR:

HV822_17320

PSPI:

PS2015_1722

SPSH:

FM037_08780 FM037_08795

ALTE:

ALT761_03050 ALT761_03053

ALTR:

BM528_03615

GAG:	Glaag_4148 Glaag_4151

PMES:

FX988_02772 FX988_02775

PAT:	Patl_0157 Patl_0160

HMI:	soil367_17600 soil367_17615

HARO:

R50071_32560 R50071_32590

MNY:	R50073_06010 R50073_44480 R50073_44510

KIM:	G3T16_03325

CVB:	R0135_13150 R0135_13165

CBRV:

R0137_00990 R0137_01005

MMAF:

GCM100_19830 GCM100_19860

MTHA:

VSX76_15095(mauB) VSX76_15110(mauA)

GAI:	IMCC3135_19700(aauA) IMCC3135_19715(aauB) IMCC3135_20060

HCS:	FF32_04535

HAMS:

NF683_05165(peaA)

HCAM:

I4484_00320(peaA)

HAXH:

J4377_16945(peaA)

HAFN:

KF947_09345(peaA)

HVN:	EI420_18585(peaA)

HNP:	SR894_19505(peaA)

ALCA:

ASALC70_04365(aauA) ASALC70_04368(aauB)

ADI:	B5T_00161 B5T_00164 B5T_00533 B5T_00536

AXE:	P40_02480 P40_02495

TWAN:

HUF19_01030

MARS:

A8C75_10935 A8C75_13875

MGEO:

CFI10_12590(peaA) CFI10_12680(peaA)

MSEC:

LN244_10005(peaA) LN244_10130(peaA)

MRZ:	KDW95_04965(peaA)

NCU:	F0U83_05970(peaA) F0U83_06020(peaA)

NJP:	NEJAP_2487(peaA)

AJP:	AMJAP_2431 AMJAP_2439

SUTR:

L0B52_07765 L0B52_07780

PSE:

NH8B_2195

CUH:	BJN34_11000 BJN34_11020 BJN34_23755 BJN34_23770

REU:	Reut_B4077 Reut_B4080

CBW:	RR42_s0274 RR42_s0277

CCUP:

BKK81_28180 BKK81_28195

CUP:	BKK80_28180 BKK80_28195

CUU:	BKK79_34095 BKK79_34110

BUR:	Bcep18194_B0601 Bcep18194_B0604

BCM:	Bcenmc03_5222 Bcenmc03_5225

BCH:	Bcen2424_5059 Bcen2424_5062

BCJ:	BCAM2301 BCAM2304

BCEN:

DM39_4450(peaA)

BCEO:

I35_6192 I35_6195

BAM:	Bamb_4469 Bamb_4472

BAC:	BamMC406_4994 BamMC406_4997

BMJ:	BMULJ_04934 BMULJ_04937

BMU:	Bmul_3580 Bmul_3583

BMK:	DM80_4965 DM80_4968(aauA)

BMUL:

NP80_4958(aauA) NP80_4961

BCT:	GEM_3380 GEM_3383 GEM_5082

BCED:

DM42_5686 DM42_5689(aauA)

BCEP:

APZ15_27590 APZ15_27605

BDL:	AK34_3489 AK34_3492(aauA)

BCON:

NL30_01045 NL30_01060

BDF:	WI26_17220

BTEI:

WS51_08585 WS51_08600

BSEM:

WJ12_31395 WJ12_31410

BPSL:

WS57_02710 WS57_02725 WS57_10400

BMEC:

WJ16_29835 WJ16_29850

BSTG:

WT74_30205 WT74_30220

BSTL:

BBJ41_25375 BBJ41_25390 BBJ41_34655 BBJ41_34670

BANN:

JFN94_21045(peaA) JFN94_29355 JFN94_29370

BARI:

NLX30_25745 NLX30_25760

BAEN:

L3V59_26030 L3V59_26045 L3V59_33245(peaA)

BSER:

R0290_18105 R0290_18120

BGF:	BC1003_3869 BC1003_3872

BYI:	BYI23_B006010 BYI23_B006040

BURK:

DM992_22970 DM992_22985

BURT:

BTHE68_33140 BTHE68_33170

BURM:

GJG85_22575 GJG85_22590

BPH:	Bphy_3809 Bphy_3812

BGE:	BC1002_1587 BC1002_1590

BCAI:

K788_0000141 K788_0000144

PHS:	C2L64_30710 C2L64_30725

PTER:

C2L65_27370 C2L65_27385

PMEG:

FNZ07_14525 FNZ07_14540

PSAA:

QEN71_14395 QEN71_14410

PPAI:

E1956_39495(peaA) E1956_39820 E1956_39835

PLAD:

PPGU16_43860 PPGU16_43890

PAZZ:

PBP221_52550 PBP221_52580

BUO:	BRPE64_BCDS07560 BRPE64_BCDS07590

CABK:

NK8_36020 NK8_36050

BUE:	BRPE67_BCDS08290 BRPE67_BCDS08320

AXY:	AXYL_02123 AXYL_02126

AXO:	NH44784_037061 NH44784_037091

AXN:	AX27061_1937 AX27061_1940

AXX:	ERS451415_01935(aauB) ERS451415_01938(aauA)

ADT:	APT56_09285 APT56_09300

AIS:	BUW96_00985 BUW96_01000

ASW:	CVS48_14415 CVS48_14430

ACHR:

C2U31_25675 C2U31_25690

ACHB:

DVB37_08350 DVB37_08365

ADY:	HLG70_12820 HLG70_12835

AAEG:

RA224_22495 RA224_22510

ASEL:

RAS12_12580 RAS12_12595 RAS12_16880(mauA) RAS12_16895

ACHA:

KYT87_08865 KYT87_08880

AVH:	WHX56_14835 WHX56_14850

ACHH:

ABFG95_23850 ABFG95_23865

PUS:	CKA81_14940 CKA81_14955

AKA:	TKWG_18915 TKWG_18935

AMIM:

MIM_c29740(mauA) MIM_c29770(mauB)

CDN:	BN940_17491 BN940_17506

AAMM:

FE795_01630 FE795_01645

ALCN:

MID00_06280 MID00_06295

HPSE:

HPF_21410

MPT:

Mpe_A2653

METP:

C1M51_14860 C1M51_14875

MFA:	Mfla_0548 Mfla_0551

MMB:	Mmol_1572 Mmol_1575

EBA:	ebA2235(qhpA) ebA5178(qhpA)

ABRE:

pbN1_04760(peaA)

APET:

ToN1_01780(qhpA1) ToN1_38420(qhpA2)

DDEN:

KI615_16540 KI615_16555

DECH:

GBK02_00305 GBK02_00320

QAU:	KI612_13825 KI612_13840

AZO:	azo1239(qhpA) azo2257(mauA) azo2260(maub)

AOA:	dqs_1354 dqs_2388 dqs_2391

AZA:	AZKH_3023(qhpA)

AZI:	AzCIB_2960

AZD:	CDA09_16050(peaA)

AZR:	CJ010_14330 CJ010_14345

TMZ:	Tmz1t_0761 Tmz1t_2982

THU:	AC731_014475 AC731_014490 AC731_017170

THAU:

C4PIVTH_0800 C4PIVTH_1325(mauA) C4PIVTH_1328(mauB)

TCL:

Tchl_2123

THK:	CCZ27_00380

TAK:	Tharo_1281

THAG:

CKCBHOJB_03182

AZQ:	G3580_00160(peaA)

MESP:

C1M53_03980 C1M53_03995

KMN:	HW532_09310(peaA)

RBS:	RHODOSMS8_03492(aauA) RHODOSMS8_03495(aauB)

MEA:	Mex_1p2770(mauB) Mex_1p2773(mauA)

MCH:	Mchl_0562 Mchl_0565

METD:

C0214_27025(mauA) C0214_27040(mauB)

HNI:	W911_02545 W911_02560

FIL:	BN1229_v1_3765(mauB) BN1229_v1_3768(mauA)

FIY:	BN1229_v1_3755(mauB) BN1229_v1_3758(mauA)

PHL:	KKY_2939 KKY_2942

PEM:	OF122_03140 OF122_03155

DNP:	N8A98_21260

BLAG:

BLTE_16570

PLEO:

OHA_1_02005(MadB) OHA_1_02008(MadA)

LAP:	ACP90_10715

LABR:

CHH27_09745(peaA)

LABP:

FJ695_27525(peaA)

LABT:

FIU93_16290

LAGG:

B0E33_22635

CAK:	Caul_0557 Caul_0560

LMD:	METH_16640

LEJ:	ETW24_23980(peaA)

LAQU:

R2C4_20365(peaA)

LEV:	ETW23_22245(peaA)

LCAE:

K3721_19810(peaA)

CID:	P73_3335 P73_3338

CEH:	CEW89_12055 CEW89_12070

PALX:

GQA70_00090(peaA)

PDE:	Pden_1703 Pden_4730 Pden_4733

PAMI:

JCM7686_0163 JCM7686_0166

PYE:	A6J80_01745(peaA) A6J80_21995(mauB) A6J80_22010(mauA)

PCON:

B0A89_14420

PZH:	CX676_03775(peaA)

PARO:

CUV01_13130(peaA)

PKD:	F8A10_18985(peaA) F8A10_20020(mauA) F8A10_20035(mauB)

PPAN:

ESD82_08405(peaA)

PSAN:

HGN31_15410(peaA)

PMEH:

JWJ88_20975(mauB) JWJ88_20990(mauA)

PSEW:

JHW44_17740(peaA)

PALP:

JHW40_00705(mauB) JHW40_00720(mauA)

PDIM:

PAF18_02540 PAF18_02555

PFEO:

E3U26_13605(mauA) E3U26_13620(mauB)

RHC:

RGUI_4107

GFU:	KM031_08500(peaA)

HML:	HmaOT1_16775 HmaOT1_16790

PGV:	SL003B_2489

THAS:

C6Y53_00400(peaA)

RHP:	LPB142_09660 LPB142_09675

HBA:	Hbal_2737 Hbal_2741

NAR:	Saro_1685 Saro_1688 Saro_3028 Saro_3818 Saro_3821

NPP:	PP1Y_Mpl7173 PP1Y_Mpl7192

NOT:	C7W88_08020(peaA) C7W88_11135 C7W88_11150 C7W88_11315 C7W88_11330

NOR:	FA702_12465(peaA) FA702_12490 FA702_12505 FA702_14885 FA702_14900 FA702_20775 FA702_20790

NDR:	HT578_04720(peaA) HT578_17915 HT578_17930

NHUM:

PQ457_03230

NCP:	U0041_07350 U0041_07365

NOVO:

IM701_09270 IM701_09285 IM701_09420 IM701_09435 IM701_12710(peaA)

SMAG:

AN936_02850 AN936_02865

SMAZ:

LH19_18390 LH19_18405

SPJJ:

V6U71_16220 V6U71_16235

SWI:	Swit_3251 Swit_3254 Swit_4363 Swit_4365

SPHD:

HY78_09285 HY78_09300

SPHM:

G432_07310 G432_07325

SSAN:

NX02_15530 NX02_15540 NX02_15555

SBIN:

SBA_ch1_01450 SBA_ch1_01480 SBA_ch1_16750

SPHR:

BSY17_2364(peaA) BSY17_2810(mauA) BSY17_2813

SHYD:

CJD35_17800 CJD35_17815 CJD35_17865(peaA)

SYA:	A6768_01720(peaA) A6768_17000 A6768_17015

SPAG:

sphantq_01412(mauB) sphantq_01415(mauA) sphantq_02895

SPCA:

GL174_07495(peaA) GL174_09035 GL174_09050

SPWT:

N6H05_09395 N6H05_09410 N6H05_23340(peaA)

SMIC:

SmB9_02170 SmB9_02200 SmB9_03800 SmB9_03830

ALB:

AEB_P3055

ERQ:	PS060_15585 PS060_15600

GFA:	MKW11_12685(mauA) MKW11_12700

GDI:	GDI0271(mauB) GDI0273(mauA) GDI1806(mauB) GDI1807(mauA)

GDJ:	Gdia_0035 Gdia_0036 Gdia_2335 Gdia_2337

GXL:	H845_1153 H845_1154 H845_1163 H845_1164 H845_1172 H845_1175

KEU:	S101446_00287(mauB) S101446_00288(mauA) S101446_00300 S101446_00301 S101446_00310 S101446_00313

APT:	APA01_04540 APA01_04570

APW:	APA42C_04540 APA42C_04570

APF:	APA03_04540 APA03_04570

APU:	APA07_04540 APA07_04570

APG:	APA12_04540 APA12_04570

APQ:	APA22_04540 APA22_04570

APX:	APA26_04540 APA26_04570

APZ:	APA32_04540 APA32_04570

APK:	APA386B_1936(mauB) APA386B_1939(mauA)

ASZ:	ASN_2153(mauB) ASN_2156(mauA)

ASV:	WG31_02710 WG31_02725

AACE:

A0U92_10600 A0U92_10615

APER:

A0U91_00065

APOM:

CPF11_05025(mauA) CPF11_05040

ATO:	CIW82_01900(mauA) CIW82_01915

AASC:

A4S02_09860

ACET:

DS739_02395 DS739_02410(mauA)

AOY:	EOV40_002245 EOV40_002260(mauA)

NTN:	D5366_10130 D5366_10145(mauA)

NGU:	QN315_00185 QN315_00190 QN315_02220 QN315_02235 QN315_08615 QN315_08625

AZZ:	DEW08_00570(peaA)

AZS:	E6C72_23175(peaA)

NCB:	C0V82_12865 C0V82_12880

MAGX:

XM1_2531

ECOG:

FIV45_14145(peaA) FIV45_16005(peaA)

GSO:	PH603_12710 PH603_12725

ABU:

Abu_0465

ABT:

ABED_0440

ABL:	A7H1H_0465

AELL:

AELL_0619

ASUI:

ASUIS_0583

AVP:	AVENP_0707 AVENP_0712

ADZ:	ADFLV_0614 ADFLV_1358

ARC:

ABLL_0607

AMOL:

AMOL_1804

AANA:

AANAER_1824

DTO:	TOL2_C06430(qhpA)

DHG:	EYB58_07975(peaA)

DVR:	Dvar_52670

MIN:	Minf_1997 Minf_2000

MKC:	kam1_317 kam1_320

MFH:	MFUM_0347 MFUM_0350(aauA)

MCAO:

IT6_04770 IT6_04785

BPIT:

BPIT_26730

TALB:

FTW19_10610(peaA)

BACO:

OXB_3506

BKN:	WDJ61_07915(peaA)

NMK:	CHR53_12040(peaA)

BBAD:

K7T73_07500(peaA)

PTL:	AOT13_15015

ANX:	ACH33_09155

ATHE:

K3F53_09935(peaA)

CPRE:

Csp1_26450(mauA)

RER:

RER_53630

REY:

O5Y_25460

REB:	XU06_25810

RQI:	C1M55_27045

RPY:	Y013_17135

RBY:	CEJ39_03535

RAV:	AAT18_05020

RRZ:	CS378_20525

RHQ:	IM25_07085

RHOD:

AOT96_07005

RRT:	4535765_00133(mauA)

RCR:	NCTC10994_01857(mauA)

RTM:	G4H71_05040

RGO:	KYT97_16925

RHOP:

D8W71_22610

RHOO:

RA302_26060

RHOU:

KZJ41_05565

RHOW:

A4U64_19525

RHOS:

QWW67_22505

RHOQ:

QWW98_22515

RHOY:

QNA09_03095

RHOH:

CPI83_03470

RHOX:

RBB84_21715

RSF:	OED52_00610

RHZG:

BO226_18235

RHBH:

A0W34_25680

RHS:	A3Q41_02066(mauA)

REQ:	REQ_44990(mauA)

DIZ:	CT688_08630

MIO:	AOA12_17540

MOJ:	D7D94_07795

» show all

Reference

1 [PMID:6246962]

Authors

de Beer R, Duine JA, Frank J, Large PJ.

Title

The prosthetic group of methylamine dehydrogenase from Pseudomonas AM1: evidence for a quinone structure.

Journal

Biochim Biophys Acta 622:370-4 (1980)
DOI:10.1016/0005-2795(80)90050-1

Reference

2 [PMID:4388687]

Authors

Eady RR, Large PJ.

Title

Purification and properties of an amine dehydrogenase from Pseudomonas AM1 and its role in growth on methylamine.

Journal

Biochem J 106:245-55 (1968)
DOI:10.1042/bj1060245

Reference

3 [PMID:5124384]

Authors

Eady RR, Large PJ.

Title

Microbial oxidation of amines. Spectral and kinetic properties of the primary amine dehydrogenase of Pseudomonas AM1.

Journal

Biochem J 123:757-71 (1971)
DOI:10.1042/bj1230757

Reference

4 [PMID:18512962]

Authors

Cavalieri C, Biermann N, Vlasie MD, Einsle O, Merli A, Ferrari D, Rossi GL, Ubbink M

Title

Structural comparison of crystal and solution states of the 138 kDa complex of methylamine dehydrogenase and amicyanin from Paracoccus versutus.

Journal

Biochemistry 47:6560-70 (2008)
DOI:10.1021/bi7023749

Reference

5 [PMID:20873742]

Authors

Meschi F, Wiertz F, Klauss L, Cavalieri C, Blok A, Ludwig B, Heering HA, Merli A, Rossi GL, Ubbink M

Title

Amicyanin transfers electrons from methylamine dehydrogenase to cytochrome c-551i via a ping-pong mechanism, not a ternary complex.

Journal

J Am Chem Soc 132:14537-45 (2010)
DOI:10.1021/ja105498m

Other DBs

ExplorEnz - The Enzyme Database:

1.4.9.1

IUBMB Enzyme Nomenclature:

1.4.9.1

ExPASy - ENZYME nomenclature database:

1.4.9.1

UM-BBD (Biocatalysis/Biodegradation Database):

1.4.9.1

BRENDA, the Enzyme Database:

1.4.9.1

DBGET integrated database retrieval system