KEGG   ENZYME: 1.4.9.1
Entry
EC 1.4.9.1                  Enzyme                                 
Name
methylamine dehydrogenase (amicyanin);
amine dehydrogenase;
primary-amine dehydrogenase;
amine: (acceptor) oxidoreductase (deaminating);
primary-amine:(acceptor) oxidoreductase (deaminating)
Class
Oxidoreductases;
Acting on the CH-NH2 group of donors;
With a copper protein as acceptor
Sysname
methylamine:amicyanin oxidoreductase (deaminating)
Reaction(IUBMB)
methylamine + H2O + 2 amicyanin = formaldehyde + NH3 + 2 reduced amicyanin [RN:R00606]
Reaction(KEGG)
R00606
Substrate
methylamine [CPD:C00218];
H2O [CPD:C00001];
amicyanin [CPD:C19671]
Product
formaldehyde [CPD:C00067];
NH3 [CPD:C00014];
reduced amicyanin [CPD:C19672]
Comment
Contains tryptophan tryptophylquinone (TTQ) cofactor. The enzyme oxidizes aliphatic monoamines and diamines, histamine and ethanolamine, but not secondary and tertiary amines, quaternary ammonium salts or aromatic amines.
History
EC 1.4.9.1 created 1978 as EC 1.4.99.3, modified 1986, transferred 2011 to EC 1.4.98.1, transferred 2011 to EC 1.4.9.1
Pathway
ec00680  Methane metabolism
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K08685  quinohemoprotein amine dehydrogenase
K15228  methylamine dehydrogenase light chain
K15229  methylamine dehydrogenase heavy chain
Genes
YMOHRD69_07500 HRD69_07515
VFUvfu_A02015 vfu_A02018
VFLAL536_13995 AL536_14010
PGHFH974_17590 FH974_17605
PMKMDS_4213 MDS_4216
PREPCA10_23420(mauB) PCA10_23450(mauA) PCA10_32510(peaA) PCA10_34250(mauB) PCA10_34280(mauA)
PFUWKF707C_23300 KF707C_23330 KF707C_25120
PCQPcP3B5_29870 PcP3B5_30140(aauA) PcP3B5_30170(aauB)
PMUIG4G71_11895(peaA) G4G71_16135 G4G71_16150
PPUPP_5602(peaA)
PPFPput_2307
PPGPputGB1_2221 PputGB1_2224 PputGB1_2475
PPWPputW619_2945
PPTPPS_2940
PPBPPUBIRD1_2330
PPIYSA_11298
PPXT1E_1613
PPUHB479_14560
PPUTL483_18160
PPUNPP4_23960(peaA)
PPUDDW66_3200
PMONX969_14050
PMOTX970_13695
PMOSO165_011585
PPJRK21_01725
PMOLCLJ08_00110(peaA)
PFLPFL_4120(peaA)
PPRCPFLCHA0_c41800
PPROPPC_4221(peaA)
PFOPfl01_2975
PMANOU5_1439
PENPSEEN2552 PSEEN2555 PSEEN2977
PDRH681_11585
PSVPVLB_12820
PKCPKB_2656(mauA) PKB_2659(mauB) PKB_2912
PCHEY04_20885
PCZPCL1606_20620
PCPJM49_10330
PALKPSAKL28_24530
PSEMTO66_21570
PSECCCOS191_2324
PSOSPOS17_4220(peaA)
PFKPFAS1_02675
PSETTHL1_3122 THL1_3125 THL1_3144 THL1_3147 THL1_3176
PSILPMA3_20245
PYMAK972_2150 AK972_2153
PKEDLD99_19475(peaA)
PUMHGP31_18355 HGP31_18370 HGP31_22220(peaA)
POJPtoMrB4_25230
PLALFXN65_11130 FXN65_11145 FXN65_11785(peaA)
PKHJLK41_14070(peaA)
PMAOPMYSY11_1047 PMYSY11_1050(mauA)
PSEPC4K39_3038 C4K39_3041
PVWHU752_028865(peaA)
PQIKH389_12680(peaA)
PXNHU772_009655 HU772_009670 HU772_012685(peaA)
PANHHU763_013035(peaA)
PASGKSS96_11100 KSS96_11115
PMAMKSS90_11360(peaA)
PAZEKSS91_08660(peaA)
PORYEJA05_14485(peaA)
PTWTUM18999_24310 TUM18999_32970 TUM18999_33000
PWYHU734_013230(peaA)
PCAVD3880_11325(peaA)
PBBAKN87_05350
SPSHFM037_08780 FM037_08795
PATPatl_0157 Patl_0160
GAGGlaag_4148 Glaag_4151
PMESFX988_02772 FX988_02775
HMIsoil367_17600 soil367_17615
KIMG3T16_03325
GAIIMCC3135_19700(aauA) IMCC3135_19715(aauB) IMCC3135_20060
HCSFF32_04535
HVNEI420_18585(peaA)
HCAMI4484_00320(peaA)
ADIB5T_00161 B5T_00164 B5T_00533 B5T_00536
AXEP40_02480 P40_02495
MARSA8C75_10935 A8C75_13875
MGEOCFI10_12590(peaA) CFI10_12680(peaA)
MSECLN244_10005(peaA) LN244_10130(peaA)
NCUF0U83_05970(peaA) F0U83_06020(peaA)
NJPNEJAP_2487(peaA)
AJPAMJAP_2431 AMJAP_2439
SUTRL0B52_07765 L0B52_07780
PSPIPS2015_1722
PSENH8B_2195
CUHBJN34_11000 BJN34_11020 BJN34_23755 BJN34_23770
REUReut_B4077 Reut_B4080
CBWRR42_s0274 RR42_s0277
CCUPBKK81_28180 BKK81_28195
CUPBKK80_28180 BKK80_28195
CUUBKK79_34095 BKK79_34110
BURBcep18194_B0601 Bcep18194_B0604
BCNBcen_3305 Bcen_3308
BCHBcen2424_5059 Bcen2424_5062
BCMBcenmc03_5222 Bcenmc03_5225
BCJBCAM2301 BCAM2304
BCENDM39_4450(peaA)
BCEOI35_6192 I35_6195
BAMBamb_4469 Bamb_4472
BACBamMC406_4994 BamMC406_4997
BMJBMULJ_04934 BMULJ_04937
BMUBmul_3580 Bmul_3583
BMKDM80_4965 DM80_4968(aauA)
BMULNP80_4958(aauA) NP80_4961
BCTGEM_3380 GEM_3383 GEM_5082
BCEDDM42_5686 DM42_5689(aauA)
BCEPAPZ15_27590 APZ15_27605
BDLAK34_3489 AK34_3492(aauA)
BCONNL30_01045 NL30_01060
BDFWI26_17220
BTEIWS51_08585 WS51_08600
BSEMWJ12_31395 WJ12_31410
BPSLWS57_02710 WS57_02725 WS57_10400
BMECWJ16_29835 WJ16_29850
BSTGWT74_30205 WT74_30220
BSTLBBJ41_25375 BBJ41_25390 BBJ41_34655 BBJ41_34670
BANNJFN94_21045(peaA) JFN94_29355 JFN94_29370
BGFBC1003_3869 BC1003_3872
BYIBYI23_B006010 BYI23_B006040
BUEBRPE67_BCDS08290 BRPE67_BCDS08320
BURKDM992_22970 DM992_22985
BPHBphy_3809 Bphy_3812
BGEBC1002_1587 BC1002_1590
BCAIK788_0000141 K788_0000144
PHSC2L64_30710 C2L64_30725
PTERC2L65_27370 C2L65_27385
PMEGFNZ07_14525 FNZ07_14540
BUOBRPE64_BCDS07560 BRPE64_BCDS07590
AXYAXYL_02123 AXYL_02126
AXONH44784_037061 NH44784_037091
AXNAX27061_1937 AX27061_1940
AXXERS451415_01935(aauB) ERS451415_01938(aauA)
ADTAPT56_09285 APT56_09300
AISBUW96_00985 BUW96_01000
ASWCVS48_14415 CVS48_14430
ACHRC2U31_25675 C2U31_25690
ACHBDVB37_08350 DVB37_08365
ADYHLG70_12820 HLG70_12835
PUSCKA81_14940 CKA81_14955
AKATKWG_18915 TKWG_18935
AMIMMIM_c29740(mauA) MIM_c29770(mauB)
CDNBN940_17491 BN940_17506
AAMMFE795_01630 FE795_01645
HPSEHPF_21410
MPTMpe_A2653
METPC1M51_14860 C1M51_14875
MFAMfla_0548 Mfla_0551
MMBMmol_1572 Mmol_1575
EBAebA2235(qhpA) ebA5178(qhpA)
ABREpbN1_04760(peaA)
APETToN1_01780(qhpA1) ToN1_38420(qhpA2)
DDENKI615_16540
QAUKI612_13825 KI612_13840
AZOazo1239(qhpA) azo2257(mauA) azo2260(maub)
AOAdqs_1354 dqs_2388 dqs_2391
AZAAZKH_3023(qhpA)
AZIAzCIB_2960
AZDCDA09_16050(peaA)
AZRCJ010_14330 CJ010_14345
TMZTmz1t_0761 Tmz1t_2982
THUAC731_014475 AC731_014490 AC731_017170
TCLTchl_2123
THKCCZ27_00380
TAKTharo_1281
AZQG3580_00160(peaA)
ABUAbu_0465
ABTABED_0440
ABLA7H1H_0465
AELLAELL_0619
ASUIASUIS_0583
AVPAVENP_0707 AVENP_0712
ADZADFLV_0614 ADFLV_1358
AMOLAMOL_1804
AANAAANAER_1824
ARCABLL_0607
DTOTOL2_C06430(qhpA)
MESPC1M53_03980 C1M53_03995
KMNHW532_09310(peaA)
RBSRHODOSMS8_03492(aauA) RHODOSMS8_03495(aauB)
MEAMex_1p2770(mauB) Mex_1p2773(mauA)
MCHMchl_0562 Mchl_0565
METDC0214_27025(mauA) C0214_27040(mauB)
HNIW911_02545 W911_02560
FILBN1229_v1_3765(mauB) BN1229_v1_3768(mauA)
FIYBN1229_v1_3755(mauB) BN1229_v1_3758(mauA)
PHLKKY_2939 KKY_2942
BLAGBLTE_16570
PLEOOHA_1_02005(MadB) OHA_1_02008(MadA)
LAPACP90_10715
LABRCHH27_09745(peaA)
LABPFJ695_27525(peaA)
LABTFIU93_16290
LAGGB0E33_22635
CAKCaul_0557 Caul_0560
LMDMETH_16640
LEJETW24_23980(peaA)
LAQUR2C4_20365(peaA)
CIDP73_3335 P73_3338
CEHCEW89_12055 CEW89_12070
PALXGQA70_00090(peaA)
RHPLPB142_09660 LPB142_09675
PDEPden_1703 Pden_4730 Pden_4733
PAMIJCM7686_0163 JCM7686_0166
PYEA6J80_01745(peaA) A6J80_21995(mauB) A6J80_22010(mauA)
PCONB0A89_14420
PZHCX676_03775(peaA)
PAROCUV01_13130(peaA)
PKDF8A10_18985(peaA) F8A10_20020(mauA) F8A10_20035(mauB)
PPANESD82_08405(peaA)
PSANHGN31_15410(peaA)
RHCRGUI_4107
GFUKM031_08500(peaA)
HMLHmaOT1_16775 HmaOT1_16790
PGVSL003B_2489
THASC6Y53_00400(peaA)
HBAHbal_2737 Hbal_2741
NARSaro_1685 Saro_1688 Saro_3028 Saro_3818 Saro_3821
NPPPP1Y_Mpl7173 PP1Y_Mpl7192
NOTC7W88_08020(peaA) C7W88_11135 C7W88_11150 C7W88_11315 C7W88_11330
NORFA702_12465(peaA) FA702_12490 FA702_12505 FA702_14885 FA702_14900 FA702_20775 FA702_20790
NDRHT578_04720(peaA) HT578_17915 HT578_17930
SMAGAN936_02850 AN936_02865
SMAZLH19_18390 LH19_18405
SWISwit_3251 Swit_3254 Swit_4363 Swit_4365
SPHDHY78_09285 HY78_09300
SPHMG432_07310 G432_07325
SSANNX02_15530 NX02_15540 NX02_15555
SPHRBSY17_2364(peaA) BSY17_2810(mauA) BSY17_2813
SHYDCJD35_17800 CJD35_17815 CJD35_17865(peaA)
SYAA6768_01720(peaA) A6768_17000 A6768_17015
SMICSmB9_02170 SmB9_02200 SmB9_03800 SmB9_03830
ALBAEB_P3055
GFAMKW11_12685(mauA) MKW11_12700
GDIGDI0271(mauB) GDI0273(mauA) GDI1806(mauB) GDI1807(mauA)
GDJGdia_0035 Gdia_0036 Gdia_2335 Gdia_2337
GXLH845_1153 H845_1154 H845_1163 H845_1164 H845_1172 H845_1175
KEUS101446_00287(mauB) S101446_00288(mauA) S101446_00300 S101446_00301 S101446_00310 S101446_00313
APTAPA01_04540 APA01_04570
APWAPA42C_04540 APA42C_04570
APFAPA03_04540 APA03_04570
APUAPA07_04540 APA07_04570
APGAPA12_04540 APA12_04570
APQAPA22_04540 APA22_04570
APXAPA26_04540 APA26_04570
APZAPA32_04540 APA32_04570
APKAPA386B_1936(mauB) APA386B_1939(mauA)
ASZASN_2153(mauB) ASN_2156(mauA)
ASVWG31_02710 WG31_02725
AACEA0U92_10600 A0U92_10615
APERA0U91_00065
APOMCPF11_05025(mauA) CPF11_05040
ATOCIW82_01900(mauA) CIW82_01915
AASCA4S02_09860
ACETDS739_02395 DS739_02410(mauA)
AOYEOV40_002245 EOV40_002260(mauA)
NTND5366_10130 D5366_10145(mauA)
AZZDEW08_00570(peaA)
NCBC0V82_12865 C0V82_12880
MAGXXM1_2531
ECOGFIV45_14145(peaA) FIV45_16005(peaA)
BBADK7T73_07500(peaA)
BACOOXB_3506
PTLAOT13_15015
NMKCHR53_12040(peaA)
ANXACH33_09155
ATHEK3F53_09935(peaA)
CPRECsp1_26450(mauA)
NWLNWFMUON74_61450
RERRER_53630
REYO5Y_25460
REBXU06_25810
RQIC1M55_27045
REQREQ_44990(mauA)
RPYY013_17135
RBYCEJ39_03535
RAVAAT18_05020
RHSA3Q41_02066(mauA)
RRZCS378_20525
RHQIM25_07085
RHODAOT96_07005
RRT4535765_00133(mauA)
RCRNCTC10994_01857(mauA)
RTMG4H71_05040
RGOKYT97_16925
DIZCT688_08630
MIOAOA12_17540
MINMinf_1997 Minf_2000
MKCkam1_317 kam1_320
BPITBPIT_26730
TALBFTW19_10610(peaA)
 » show all
Reference
1  [PMID:6246962]
  Authors
de Beer R, Duine JA, Frank J, Large PJ.
  Title
The prosthetic group of methylamine dehydrogenase from Pseudomonas AM1: evidence for a quinone structure.
  Journal
Biochim Biophys Acta 622:370-4 (1980)
DOI:10.1016/0005-2795(80)90050-1
Reference
2  [PMID:4388687]
  Authors
Eady RR, Large PJ.
  Title
Purification and properties of an amine dehydrogenase from Pseudomonas AM1 and its role in growth on methylamine.
  Journal
Biochem J 106:245-55 (1968)
DOI:10.1042/bj1060245
Reference
3  [PMID:5124384]
  Authors
Eady RR, Large PJ.
  Title
Microbial oxidation of amines. Spectral and kinetic properties of the primary amine dehydrogenase of Pseudomonas AM1.
  Journal
Biochem J 123:757-71 (1971)
DOI:10.1042/bj1230757
Reference
4  [PMID:18512962]
  Authors
Cavalieri C, Biermann N, Vlasie MD, Einsle O, Merli A, Ferrari D, Rossi GL, Ubbink M
  Title
Structural comparison of crystal and solution states of the 138 kDa complex of methylamine dehydrogenase and amicyanin from Paracoccus versutus.
  Journal
Biochemistry 47:6560-70 (2008)
DOI:10.1021/bi7023749
Reference
5  [PMID:20873742]
  Authors
Meschi F, Wiertz F, Klauss L, Cavalieri C, Blok A, Ludwig B, Heering HA, Merli A, Rossi GL, Ubbink M
  Title
Amicyanin transfers electrons from methylamine dehydrogenase to cytochrome c-551i via a ping-pong mechanism, not a ternary complex.
  Journal
J Am Chem Soc 132:14537-45 (2010)
DOI:10.1021/ja105498m
Other DBs
ExplorEnz - The Enzyme Database: 1.4.9.1
IUBMB Enzyme Nomenclature: 1.4.9.1
ExPASy - ENZYME nomenclature database: 1.4.9.1
UM-BBD (Biocatalysis/Biodegradation Database): 1.4.9.1
BRENDA, the Enzyme Database: 1.4.9.1

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