KEGG   ENZYME: 1.4.99.5
Entry
EC 1.4.99.5                 Enzyme                                 

Name
glycine dehydrogenase (cyanide-forming);
hydrogen cyanide synthase;
HCN synthase
Class
Oxidoreductases;
Acting on the CH-NH2 group of donors;
With unknown physiological acceptors
Sysname
glycine:acceptor oxidoreductase (hydrogen-cyanide-forming)
Reaction(IUBMB)
glycine + 2 acceptor = hydrogen cyanide + CO2 + 2 reduced acceptor [RN:R05704]
Reaction(KEGG)
R05704 > R00374
Substrate
glycine [CPD:C00037];
acceptor [CPD:C00028]
Product
hydrogen cyanide [CPD:C01326];
CO2 [CPD:C00011];
reduced acceptor [CPD:C00030]
Comment
The enzyme from Pseudomonas sp. contains FAD. The enzyme is membrane-bound, and the 2-electron acceptor is a component of the respiratory chain. The enzyme can act with various artificial electron acceptors, including phenazine methosulfate.
History
EC 1.4.99.5 created 2002
Pathway
ec00460  Cyanoamino acid metabolism
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K10814  hydrogen cyanide synthase HcnA
K10815  hydrogen cyanide synthase HcnB
K10816  hydrogen cyanide synthase HcnC
Genes
LVE: 103087047
VEX: VEA_000037
VHR: AL538_20020
VCY: IX92_17720 IX92_17725 IX92_17730
VCT: JV59_21340 JV59_21345 JV59_21350
SALY: E8E00_15310 E8E00_15315 E8E00_15320
SCOT: HBA18_15205 HBA18_15210 HBA18_15215
PAE: PA2193(hcnA) PA2194(hcnB) PA2195(hcnC)
PAEV: N297_2266 N297_2267 N297_2268
PAEI: N296_2266 N296_2267 N296_2268
PAU: PA14_36310(hcnC) PA14_36320(hcnB) PA14_36330(hcnA)
PAP: PSPA7_3101(hcnC) PSPA7_3102(hcnB) PSPA7_3103(hcnA)
PAG: PLES_31301(hcnC) PLES_31311(hcnB) PLES_31321(hcnA)
PAF: PAM18_2840(hcnC) PAM18_2841(hcnB) PAM18_2842(hcnA)
PNC: NCGM2_3175(hcnA) NCGM2_3176(hcnB) NCGM2_3177(hcnC)
PAEU: BN889_02406(hcnA) BN889_02407(hcnB) BN889_02408(hcnC)
PFL: PFL_2577(hcnA) PFL_2578(hcnB) PFL_2579(hcnC)
PPRO: PPC_2622(hcnA) PPC_2623(hcnB) PPC_2624(hcnC)
PFO: Pfl01_3514(hcnC) Pfl01_3515(hcnB) Pfl01_3516(hcnA)
PFE: PSF113_2367(hcnA) PSF113_2368(hcnB) PSF113_2369(hcnC)
PEN: PSEEN5520(hcnC) PSEEN5521(hcnB) PSEEN5522(hcnA)
PSEC: CCOS191_5263(hcnC) CCOS191_5264(hcnB) CCOS191_5265(hcnA)
PSOS: POS17_2580(hcnA) POS17_2581(hcnB) POS17_2582(hcnC)
PSEN: PNC201_13070(hcnC) PNC201_13075(hcnB) PNC201_13080(hcnA)
CVI: CV_1682(hcnC) CV_1683(hcnB) CV_1684(hcnA)
CHAE: CH06BL_12990(hcnC) CH06BL_13000(hcnB) CH06BL_13010(hcnA)
BPAR: BN117_3795
BPA: BPP0901
BBR: BB0994
AXX: ERS451415_00042(thiO_2)
MES: Meso_3638
SMD: Smed_5072
AGC: BSY240_56
RID: RIdsm_01233(hcnC)
SPHR: BSY17_3519
 » show all
Reference
1  [PMID:234422]
  Authors
Wissing F.
  Title
Cyanide production from glycine by a homogenate from a Pseudomonas species.
  Journal
J Bacteriol 121:695-9 (1975)
DOI:10.1128/JB.121.2.695-699.1975
Reference
2  [PMID:233722]
  Authors
Castric PA.
  Title
Glycine metabolism by Pseudomonas aeruginosa: hydrogen cyanide biosynthesis.
  Journal
J Bacteriol 130:826-31 (1977)
DOI:10.1128/JB.130.2.826-831.1977
  Sequence
Reference
3  [PMID:9620970]
  Authors
Laville J, Blumer C, Von Schroetter C, Gaia V, Defago G, Keel C, Haas D.
  Title
Characterization of the hcnABC gene cluster encoding hydrogen cyanide synthase and anaerobic regulation by ANR in the strictly aerobic biocontrol agent Pseudomonas fluorescens CHA0.
  Journal
J Bacteriol 180:3187-96 (1998)
DOI:10.1128/JB.180.12.3187-3196.1998
  Sequence
Reference
4  [PMID:10763748]
  Authors
Blumer C, Haas D.
  Title
Mechanism, regulation, and ecological role of bacterial cyanide biosynthesis.
  Journal
Arch Microbiol 173:170-7 (2000)
DOI:10.1007/s002039900127
Other DBs
ExplorEnz - The Enzyme Database: 1.4.99.5
IUBMB Enzyme Nomenclature: 1.4.99.5
ExPASy - ENZYME nomenclature database: 1.4.99.5
BRENDA, the Enzyme Database: 1.4.99.5

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