KEGG   ENZYME: 1.4.99.5
Entry
EC 1.4.99.5                 Enzyme                                 
Name
glycine dehydrogenase (cyanide-forming);
hydrogen cyanide synthase;
HCN synthase
Class
Oxidoreductases;
Acting on the CH-NH2 group of donors;
With unknown physiological acceptors
Sysname
glycine:acceptor oxidoreductase (hydrogen-cyanide-forming)
Reaction(IUBMB)
glycine + 2 acceptor = hydrogen cyanide + CO2 + 2 reduced acceptor [RN:R05704]
Reaction(KEGG)
R05704 > R00374
Substrate
glycine [CPD:C00037];
acceptor [CPD:C00028]
Product
hydrogen cyanide [CPD:C01326];
CO2 [CPD:C00011];
reduced acceptor [CPD:C00030]
Comment
The enzyme from Pseudomonas sp. contains FAD. The enzyme is membrane-bound, and the 2-electron acceptor is a component of the respiratory chain. The enzyme can act with various artificial electron acceptors, including phenazine methosulfate.
History
EC 1.4.99.5 created 2002
Pathway
ec00460  Cyanoamino acid metabolism
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K10814  hydrogen cyanide synthase HcnA
K10815  hydrogen cyanide synthase HcnB
K10816  hydrogen cyanide synthase HcnC
Genes
VCYIX92_17720 IX92_17725 IX92_17730
VCTJV59_21340 JV59_21345 JV59_21350
VAQFIV01_19435(hcnC)
VNPKW548_21885 KW548_21890 KW548_21895
SALYE8E00_15310 E8E00_15315 E8E00_15320
SCOTHBA18_15205 HBA18_15210 HBA18_15215
SPRON7E60_17180 N7E60_17185 N7E60_17190
PAEPA2193(hcnA) PA2194(hcnB) PA2195(hcnC)
PAEVN297_2266 N297_2267 N297_2268
PAEIN296_2266 N296_2267 N296_2268
PAUPA14_36310(hcnC) PA14_36320(hcnB) PA14_36330(hcnA)
PAPPSPA7_3101(hcnC) PSPA7_3102(hcnB) PSPA7_3103(hcnA)
PAGPLES_31301(hcnC) PLES_31311(hcnB) PLES_31321(hcnA)
PAFPAM18_2840(hcnC) PAM18_2841(hcnB) PAM18_2842(hcnA)
PNCNCGM2_3175(hcnA) NCGM2_3176(hcnB) NCGM2_3177(hcnC)
PDKPADK2_14680 PADK2_14685 PADK2_14690
PSGG655_14175 G655_14180 G655_14185
PRPM062_11375 M062_11380 M062_11385
PAEPPA1S_14755 PA1S_14760 PA1S_14765
PAERPA1R_gp5664 PA1R_gp5665 PA1R_gp5666
PAEMU769_14355 U769_14360 U769_14365
PAELT223_15985 T223_15990 T223_15995
PAESSCV20265_3179 SCV20265_3180 SCV20265_3181
PAEUBN889_02406(hcnA) BN889_02407(hcnB) BN889_02408(hcnC)
PAEGAI22_19020 AI22_19025 AI22_19030
PAECM802_2263 M802_2264 M802_2265
PAEOM801_2265 M801_2266 M801_2267
PPAAB7D75_14070 B7D75_14075 B7D75_14080
PMOLCLJ08_13065 CLJ08_13070 CLJ08_13075
PMOSO165_023000 O165_023005 O165_023010
PFLPFL_2577(hcnA) PFL_2578(hcnB) PFL_2579(hcnC)
PPRCPFLCHA0_c26420(hcnA) PFLCHA0_c26430(ooxA) PFLCHA0_c26440
PPROPPC_2622(hcnA) PPC_2623(hcnB) PPC_2624(hcnC)
PFEPSF113_2367(hcnA) PSF113_2368(hcnB) PSF113_2369(hcnC)
PFOPfl01_3514(hcnC) Pfl01_3515(hcnB) Pfl01_3516(hcnA)
PMANOU5_6068 OU5_6069 OU5_6070
PCGAXG94_04380 AXG94_04385 AXG94_04390
PMEDE3Z27_16665(hcnC) E3Z27_16670(hcnB) E3Z27_16675(hcnA)
PKRAYO71_19305 AYO71_19310 AYO71_19315
PENPSEEN5520(hcnC) PSEEN5521(hcnB) PSEEN5522(hcnA)
PBAPSEBR_a3359 PSEBR_a3360 PSEBR_a3361
PBCCD58_12305 CD58_12310 CD58_12315
PCHEY04_11740 EY04_11745 EY04_11750
PCZPCL1606_36260 PCL1606_36270 PCL1606_36280
PCPJM49_18105 JM49_18110 JM49_18115
PCHPC4K32_2519 C4K32_2520 C4K32_2521
PFZAV641_13585 AV641_13590 AV641_13595
PLQAA042_19290 AA042_19295 AA042_19300
PALKPSAKL28_52480 PSAKL28_52490 PSAKL28_52500
PSWLK03_05925 LK03_05930
PPVNJ69_19230 NJ69_19235 NJ69_19240
PSEMTO66_12560 TO66_12565 TO66_12570
PSECCCOS191_5263(hcnC) CCOS191_5264(hcnB) CCOS191_5265(hcnA)
PPSYAOC04_10125 AOC04_10130 AOC04_10135
PSOSPOS17_2580(hcnA) POS17_2581(hcnB) POS17_2582(hcnC)
PKEDLD99_17555 DLD99_17560 DLD99_17565
PGYAWU82_10070 AWU82_10075 AWU82_10080
PVKEPZ47_12195(hcnA) EPZ47_12200(hcnB) EPZ47_12205(hcnC)
PBZGN234_05950(hcnC) GN234_05955(hcnB) GN234_05960(hcnA)
PATAJWU58_10955 JWU58_10960(hcnB) JWU58_10965(hcnC)
PDWBV82_2272(hcnC) BV82_2273(hcnB) BV82_2274(hcnA)
PZEHU754_011890(hcnA) HU754_011895(hcnB) HU754_011900(hcnC)
PMOEHV782_018050(hcnC) HV782_018055(hcnB) HV782_018060
PSEPC4K39_2594 C4K39_2595 C4K39_2596
PPIIQL104_13580(hcnA) QL104_13585(hcnB) QL104_13590(hcnC)
PVWHU752_010780(hcnA) HU752_010785(hcnB) HU752_010790(hcnC)
PXAKSS93_02125(hcnA) KSS93_02130(hcnB) KSS93_02135(hcnC)
PTZHU718_018835(hcnC) HU718_018840(hcnB) HU718_018845(hcnA)
PQIKH389_26980(hcnC) KH389_26985(hcnB) KH389_26990(hcnA)
PXNHU772_024760(hcnC) HU772_024765(hcnB) HU772_024770(hcnA)
PHVHU739_001835 HU739_001840(hcnB) HU739_001845(hcnC)
PFAKKSS94_26940(hcnC) KSS94_26945(hcnB) KSS94_26950(hcnA)
PMUYKSS95_03225(hcnA) KSS95_03230(hcnB) KSS95_03235(hcnC)
PMAMKSS90_25305(hcnC) KSS90_25310(hcnB) KSS90_25315(hcnA)
PALVKSS97_11955(hcnA) KSS97_11960(hcnB) KSS97_11965(hcnC)
PORYEJA05_27955(hcnC) EJA05_27960(hcnB) EJA05_27965(hcnA)
PTKEXN22_01415(hcnC) EXN22_01420(hcnB) EXN22_01425(hcnA)
PZAHU749_018035(hcnC) HU749_018040(hcnB) HU749_018045(hcnA)
PIEHU724_017695(hcnC) HU724_017700(hcnB) HU724_017705
PWYHU734_001010(hcnA) HU734_001015(hcnB) HU734_001020(hcnC)
PGFJ0G10_18790(hcnC) J0G10_18795(hcnB) J0G10_18800(hcnA)
PSIINF676_16570(hcnC) NF676_16575(hcnB) NF676_16580
PSIHLOY51_26750(hcnC) LOY51_26755(hcnB) LOY51_26760(hcnA)
PCASLOY40_11445(hcnA) LOY40_11450(hcnB) LOY40_11455(hcnC)
PFITKJY40_18150(hcnC) KJY40_18155(hcnB) KJY40_18160(hcnA)
PNBNK667_14425(hcnC) NK667_14430(hcnB) NK667_14435(hcnA)
PCUCPSH97_17330(hcnC) PSH97_17335(hcnB) PSH97_17340
THATH3N35_17380 H3N35_17385 H3N35_17390
TACTSG35_018375 SG35_018380 SG35_018385
TVDSG34_010900 SG34_010905 SG34_010910
PRRAT705_00060 AT705_00065 AT705_00070
PLZS4054249_17860 S4054249_17865 S4054249_17870
PALNB0W48_15825 B0W48_15830 B0W48_15835
PPISB1L02_03835 B1L02_03840
PSENPNC201_13070(hcnC) PNC201_13075(hcnB) PNC201_13080(hcnA)
PVBJ5X90_12455 J5X90_12460 J5X90_12465
PSAZPA25_08410(hcnC) PA25_08420(hcnB) PA25_08430(hcnA)
PFLICTT31_13375 CTT31_13380 CTT31_13385
PMAZR5H13_13860 R5H13_13865 R5H13_13870
HAXIHAALTHF_45770n
CVICV_1682(hcnC) CV_1683(hcnB) CV_1684(hcnA)
CVCBKX93_21640 BKX93_21645 BKX93_21650
CHROCXB49_13965 CXB49_13970 CXB49_13975
CHRIDK842_15670 DK842_15675 DK842_15680
CHRBDK843_21200 DK843_21205 DK843_21210
CRZD1345_05980 D1345_05985 D1345_05990
CHAECH06BL_12990(hcnC) CH06BL_13000(hcnB) CH06BL_13010(hcnA)
SECHB18_22880 B18_22885 B18_22890
STELSTAQ_37370(ooxA)
MSAGGCM10017556_23080(hcnC)
 » show all
Reference
1  [PMID:234422]
  Authors
Wissing F.
  Title
Cyanide production from glycine by a homogenate from a Pseudomonas species.
  Journal
J Bacteriol 121:695-9 (1975)
DOI:10.1128/JB.121.2.695-699.1975
Reference
2  [PMID:233722]
  Authors
Castric PA
  Title
Glycine metabolism by Pseudomonas aeruginosa: hydrogen cyanide biosynthesis.
  Journal
J Bacteriol 130:826-31 (1977)
DOI:10.1128/JB.130.2.826-831.1977
  Sequence
Reference
3  [PMID:9620970]
  Authors
Laville J, Blumer C, Von Schroetter C, Gaia V, Defago G, Keel C, Haas D
  Title
Characterization of the hcnABC gene cluster encoding hydrogen cyanide synthase and anaerobic regulation by ANR in the strictly aerobic biocontrol agent Pseudomonas fluorescens CHA0.
  Journal
J Bacteriol 180:3187-96 (1998)
DOI:10.1128/JB.180.12.3187-3196.1998
  Sequence
Reference
4  [PMID:10763748]
  Authors
Blumer C, Haas D.
  Title
Mechanism, regulation, and ecological role of bacterial cyanide biosynthesis.
  Journal
Arch Microbiol 173:170-7 (2000)
DOI:10.1007/s002039900127
Other DBs
ExplorEnz - The Enzyme Database: 1.4.99.5
IUBMB Enzyme Nomenclature: 1.4.99.5
ExPASy - ENZYME nomenclature database: 1.4.99.5
BRENDA, the Enzyme Database: 1.4.99.5

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