KEGG   ENZYME: 1.5.1.20
Entry
EC 1.5.1.20                 Enzyme                                 

Name
methylenetetrahydrofolate reductase [NAD(P)H];
MTHFR (gene name)
Class
Oxidoreductases;
Acting on the CH-NH group of donors;
With NAD+ or NADP+ as acceptor
Sysname
5-methyltetrahydrofolate:NAD(P)+ oxidoreductase
Reaction(IUBMB)
5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H + H+ [RN:R01224 R07168]
Reaction(KEGG)
R01224 R07168
Substrate
5-methyltetrahydrofolate [CPD:C00440];
NAD+ [CPD:C00003];
NADP+ [CPD:C00006]
Product
5,10-methylenetetrahydrofolate [CPD:C00143];
NADH [CPD:C00004];
NADPH [CPD:C00005];
H+ [CPD:C00080]
Comment
A flavoprotein (FAD). The enzyme catalyses the reversible conversion of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate, playing an important role in folate metabolism by regulating the distribution of one-carbon moieties between cellular methylation reactions and nucleic acid synthesis. This enzyme, characterized from Protozoan parasites of the genus Leishmania, is unique among similar characterized eukaryotic enzymes in that it lacks the C-terminal allosteric regulatory domain (allowing it to catalyse a reversible reaction) and uses NADH and NADPH with equal efficiency under physiological conditions. cf. EC 1.5.1.53, methylenetetrahydrofolate reductase (NADPH); EC 1.5.1.54, methylenetetrahydrofolate reductase (NADH); and EC 1.5.7.1, methylenetetrahydrofolate reductase (ferredoxin).
History
EC 1.5.1.20 created 1978 as EC 1.1.1.171, transferred 1984 to EC 1.5.1.20 (EC 1.7.99.5 incorporated 2005), modified 2005., modified 2021
Pathway
ec00670  One carbon pool by folate
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K25009  methylenetetrahydrofolate reductase [NAD(P)H]
Genes
LMA: LMJF_36_6390
LIF: LINJ_36_6670
LDO: LDBPK_366670
LMI: LMXM_36_6390
LBZ: LBRM_35_6720
LPAN: LPMP_356560
Reference
1  [PMID:17032644]
  Authors
Vickers TJ, Orsomando G, de la Garza RD, Scott DA, Kang SO, Hanson AD, Beverley SM.
  Title
Biochemical and genetic analysis of methylenetetrahydrofolate reductase in Leishmania metabolism and virulence.
  Journal
J Biol Chem 281:38150-8 (2006)
DOI:10.1074/jbc.M608387200
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.5.1.20
IUBMB Enzyme Nomenclature: 1.5.1.20
ExPASy - ENZYME nomenclature database: 1.5.1.20
BRENDA, the Enzyme Database: 1.5.1.20
CAS: 71822-25-8

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