KEGG   ENZYME: 1.5.1.28
Entry
EC 1.5.1.28                 Enzyme                                 
Name
opine dehydrogenase;
(2S)-2-{[1-(R)-carboxyethyl]amino}pentanoate dehydrogenase (NAD+, L-aminopentanoate-forming)
Class
Oxidoreductases;
Acting on the CH-NH group of donors;
With NAD+ or NADP+ as acceptor
Sysname
(2S)-2-{[1-(R)-carboxyethyl]amino}pentanoate:NAD+ oxidoreductase (L-aminopentanoate-forming)
Reaction(IUBMB)
(2S)-2-{[1-(R)-carboxyethyl]amino}pentanoate + NAD+ + H2O = L-2-aminopentanoic acid + pyruvate + NADH + H+ [RN:R03732]
Reaction(KEGG)
R03732
Substrate
(2S)-2-{[1-(R)-carboxyethyl]amino}pentanoate [CPD:C06326];
NAD+ [CPD:C00003];
H2O [CPD:C00001]
Product
L-2-aminopentanoic acid [CPD:C01826];
pyruvate [CPD:C00022];
NADH [CPD:C00004];
H+ [CPD:C00080]
Comment
In the forward direction, the enzyme from Arthrobacter sp. acts also on secondary amine dicarboxylates such as N-(1-carboxyethyl)methionine and N-(1-carboxyethyl)phenylalanine. Dehydrogenation forms an imine, which dissociates to the amino acid and pyruvate. In the reverse direction, the enzyme acts also on neutral amino acids as an amino donor. They include L-amino acids such as 2-aminopentanoic acid, 2-aminobutyric acid, 2-aminohexanoic acid, 3-chloroalanine, O-acetylserine, methionine, isoleucine, valine, phenylalanine, leucine and alanine. The amino acceptors include 2-oxoacids such as pyruvate, oxaloacetate, glyoxylate and 2-oxobutyrate.
History
EC 1.5.1.28 created 1999
Orthology
K04940  opine dehydrogenase
Genes
KPYKPNIH31_18350
SYMK6K13_09415
PPUJE2566_14255
DDADd703_1794
PSTABGK56_13885
VSCVSVS12_01102
VGIMID13_16255
PPRPBPRB0771
PGBH744_2c1740
PDSCAY62_19020
PGHFH974_23115
PPUPP_4452
PPJRK21_02019
PSWLK03_15960
PUMHGP31_10745
PTWTUM18999_33620
ADVDJ533_11660
ARJDOM24_00265 DOM24_03895
AGUAS4_15340
ADSFPL17_09170
ABERBSR55_08030
AGYLFPL18_01540
SSESsed_0671
SVOSVI_0643
SJASJ2017_1874
SBKSHEWBE_4245
SPSHFM037_04685
SEURFM038_022115
COMCMT41_09325 CMT41_09330
CBERB5D82_15345
PSYPCNPT3_02670 PCNPT3_07190
KIMG3T16_14950
ALGAQULUS_22390(odh)
ASIPAQUSIP_24770(odh)
LLOLLO_2372
LSHCAB17_17165
THIMKFB96_06605
HAKKO116_00217
HHHCLM76_06950
HOLHORIV_70190
HTTHZS52_05580
HPROLMS44_23015
ASRWL1483_1877
SDFACG33_04745
VMSLVJ82_03840
NBACUN60_04950
RSCRCFBP_20774 RCFBP_20776
BTEBTH_II0336
BTQBTQ_3630
BTJBTJ_4666
BTZBTL_5454
BTVBTHA_4749
BTHEBTN_5124
BTHMBTRA_5393
BTHADR62_5254
BTHLBG87_5460
BOKDM82_6318
BOCBG90_4356
BURBcep18194_B2489
BCMBcenmc03_3894
BCHBcen2424_3626
BCJBCAM0655
BCENDM39_4502
BCEOI35_4551
BAMBamb_5362
BACBamMC406_3532
BMUBmul_5067
BMKDM80_4242
BMULNP80_3656
BCEDDM42_4435
BCEPAPZ15_21185
BPYRABD05_18580
BCONNL30_22575
BUBBW23_6022
BTEIWS51_01775
BSEMWJ12_23245
BPSLWS57_10650
BMECWJ16_22265
BSTGWT74_18810
BSTLBBJ41_37385
BANNJFN94_20785
BURKDM992_28810
BCAIK788_0004364
PSPWBJG93_34305
PTERC2L65_38055
AXYAXYL_04301
AXONH44784_057821
AXNAX27061_4043
AXXERS451415_04062(odh)
AISBUW96_10710
ASWCVS48_23015
ACHRC2U31_16515
ACHBDVB37_17400
PUDG9Q38_13250
AFAUZ73_09110
AFQAFA_01515
AAQUD3M96_01375
AAMMFE795_01445
PIGEGT29_20880 EGT29_21235
PACRFXN63_16025
RHYRD110_22480
RHFEUB48_08085
AAVAave_0569
ACKC380_20575
ACRABSY15_2487 BSY15_2488
VEIVeis_1521 Veis_1563
VAPVapar_0420 Vapar_1485 Vapar_6050
VPEVarpa_1641
VPDVAPA_1c05000 VAPA_1c15770
VBOCKY39_25135
VAMC4F17_10965 C4F17_16570
ADNAlide_4210
ADKAlide2_4551
LIML103DPR2_02654(odh)
HTNKI616_22000
DRGH9K76_01095
SIMPC6571_14675
MNRACZ75_11700
AZRCJ010_14285
GLOGlov_2392
DRTDret_0075
DWDDSCW_62580
DALKDSCA_25840 DSCA_56040
CFUSCYFUS_003401
MESMeso_2393
AAKAA2016_2193 AA2016_5625 AA2016_6453
AMIHCO731_00337(odh_1) CO731_05356(odh_2) CO731_05401(odh_3)
PHTBLM14_16170 BLM14_27490
NIYFQ775_03900
NKIKW403_06385 KW403_18915
SMESM_b20286
SMKSinme_3886 Sinme_6865
SMQSinmeB_4383
SMXSM11_pD1324
SMIBN406_06379
SMEGC770_GR4pD1310
SMELSM2011_b20286
SMERDU99_31935
SMDSmed_3845
RHINGR_a00740 NGR_b03550
SAMESAMCFNEI73_pC0251
SINOSS05631_a45530 SS05631_b51320
EAHFA04_25690
ESJSJ05684_a40760 SJ05684_b45290
EMXFKV68_24795
ATUAtu5418
ARAArad_14152
ATAAWN88_07295
ATFAch5_51210
AVIAvi_8271
AGTEYD00_17880
ALFCFBP5473_15110 CFBP5473_23920
ARUIG6M88_15545
RTRRTCIAT899_PC01150
RIRBN877_II0850
RPUSCFBP5875_18050
RHVBA939_22590
NENNCHU2750_46460(odh) NCHU2750_55530(odh)
SHZshn_28825
ABAWD5400_06845 D5400_08020
PSCQKHQ08_04000
BJAblr6086(blr6086)
BJUBJ6T_36230
BJPRN69_17565
BRABRADO2316
BBTBBta_2679
BRSS23_21890
AOLS58_43630 S58_52810
BRCBCCGELA001_25845
BRADBF49_5547
BICLMTR13_05335
BROBRAD285_5026 BRAD285_6496
BOTCIT37_23850
BRQCIT40_22410
BGQX265_12350
BGZXH91_11125
BSYMCIT39_21605
BBETF8237_26020
BARHWN72_33995
BVZBRAD3257_3043
BDGLPJ38_32250
RBKE0H22_08225
TRBHB776_01610
BOPAXW83_12780
BVVBHK69_21260
BOFFQV39_15270
CHELAL346_18015
CDQBOQ54_09490
MEYTM49_08060
MAADAZF01_00385
MLUTJET14_01590
AALAIGS74_04160
AALMLUX29_04765 LUX29_20630
PSINCAK95_27035
SIWGH266_20145
SILSPO1466
RUAD1823_08260
RUTFIU92_04995(odh)
RDERD1_0281
RLIRLO149_c043040
OATOAN307_c17950
YPACCEW88_03990 CEW88_18790
PABYGa0080574_TMP4526
SDOSD1155_01160
AHTANTHELSMS3_01768(odh)
SAGUCDO87_23075
MALUKU6B_15920
PZHCX676_20540
GFUKM031_19610
PSEBEOK75_18505
PPRUFDP22_13150
PALWPSAL_036070(odh)
PPAFI8N54_06975
THASC6Y53_18015
NDRHT578_14495
SWISwit_0736
SPHDHY78_23015
SRFLHU95_05185
SVCSTVA_11790(odh)
AZZDEW08_27845
THACCSC3H3_23765
SBFJCM31447_15750
BCERBCK_09040
BFDNCTC4823_00639(odh)
BDAFSZ17_20775
CFIRNAF01_04355
AXLAXY_19850
LYZDCE79_01465
VIGBKP57_15195
VPTKBP50_14755
LAOAOX59_18555
SCIAHUG15_01365
NDTL1999_05710
SAUSA2095
SAVSAV2302
SAWSAHV_2286
SAHSaurJH1_2368
SAJSaurJH9_2325
SAMMW2220
SASSAS2192
SARSAR2386
SACSACOL2293
SAXUSA300HOU_2283
SAASAUSA300_2251
SAOSAOUHSC_02574
SAENWMN_2201
SADSAAV_2363
SUUM013TW_2258
SUVSAVC_10365
SUESAOV_2341c
SUJSAA6159_02205
SUKSAA6008_02337
SUCECTR2_2161
SUTSAT0131_02480
SUQHMPREF0772_10896
SUZMS7_2319
SUDST398NM01_2352
SUXSAEMRSA15_21990
SUWSATW20_24320
SUGSAPIG2352
SUFSARLGA251_20840
SAUASAAG_00127
SAUERSAU_002134
SAUNSAKOR_02267
SAUSSA40_2047
SAUUSA957_2131
SAUGSA268_2202
SAUZSAZ172_2401
SAUTSAI1T1_2016990
SAUJSAI2T2_1017000
SAUKSAI3T3_1016990
SAUQSAI4T8_1017000
SAUVSAI7S6_1016990
SAUWSAI5S5_1016930
SAUXSAI6T6_1016940
SAUYSAI8T7_1016970
SAUFX998_2283
SABSAB2174c
SUYSA2981_2240
SAUBC248_2341
SAUMBN843_23380
SAUCCA347_2380
SAURSABB_01371
SAUIAZ30_12130
SAUDCH52_07465
SAMSNI36_11355
SUHSAMSHR1132_21260
SERSERP1882
SEPSE_1874
SEPPSEB_01886
SEPSDP17_824
SHASH0757
SHHShL2_00657
SSPSSP0611
SCASCA_1793
SLGSLGD_00729
SLNSLUG_07290
SSDSPSINT_1961
SDTSPSE_0521
SDPNCTC12225_00601(odh)
SWAA284_03050
SPASSTP1_0780
SXYBE24_08925
SXLSXYLSMQ121_0644
SXOSXYL_00654
SHUSHYC_02925
SCAPAYP1020_1476(odh)
SSCHLH95_02730
SSCZRN70_03005
SAGQEP23_07195
SEQOSE1039_19640
SSIFAL483_00830
SCVA4G25_11160
SPETCEP67_05390
SLZB5P37_03565
SCOHBZ166_07495
SNLBJD96_03385
SKLC7J89_04615
SFQC7J90_05630
SHOMEGX58_07295
SMUSC7J88_05685
SCARDWB96_03250
SCHRDWB92_02770
SARLSAP2_06870
SPICSAMEA4384060_0733(odh)
SSHNCTC13712_02248(odh)
SSIMSAMEA4384339_2129(odh)
SDBCNQ82_10985
SLLOISP08_03280
SAULI6G39_02675
SSACI6I31_01420
SRAILN051_02400
SPSDJMB28_03725
STASHYI43_03580
SSCUCEP64_12120
SFFFOB90_05025
SSTESAMEA4384403_2425(odh)
MLENH3V22_09710
MVTI6J10_11375
SHVAAT16_01855 AAT16_04580 AAT16_10350
SBACBVH56_07700
JEAJEM45_08205 JEM45_09165
PSABPSAB_03580
PBJVN24_18250
PKUAUO94_08225 AUO94_14135
PRTAUC31_14985
PPLABBI15_07490
PFAEAJGP001_03050 AJGP001_16835
PDECH1Q58_08115 H1Q58_12350
KZONCTC404_02705(odh)
SPSYAZE41_18985
SPORSporoP33_01745
SURESporoP32a_10975
RSTATY39_13220
PANCE2636_12305 E2636_15760
LCZLCAZH_0417
LCWBN194_04600
LCLLOCK919_0480
LPILBPG_02566
LPAPLBPC_0352
LCBLCABL_04530(AGR_pAT_610)
LCXLCA12A_2399
LRHLGG_00477
LFFLBFF_0980
LRMLRC_15610
ESSATZ33_18060
EAVEH197_12370
ERAFJ9537_18205
AVSAWM76_04905
JDABW727_101397(odh_1) BW727_101917(odh_2)
JEHEJN90_09045
JARG7057_07950
JPOG7058_09100
CLTCM240_3066
CEUA7L45_04215
CGASJ1C67_09700
AMTAmet_2956
GFEGferi_04555 Gferi_15455
CRSFQB35_01385 FQB35_02255 FQB35_03370 FQB35_03395 FQB35_14565
CPRFK7H06_04865 K7H06_06220 K7H06_10295 K7H06_15035 K7H06_15900
RALRumal_0438
CAPREQM14_09355
OBJEIO64_01270
ACOLK5I23_05245 K5I23_13220
BYLA4V09_17285
BHANCGC63_00480
BLABEYS05_03445
ARFAR1Y2_3063
HSDSD1D_0903
SINTK5I26_14940
WHJH9Q79_08845
ROCHF520_07675
TEMJW646_04940
TGCL0P85_03420
TEBT8CH_0355(odh) T8CH_2851
IBRNQ514_12015
STHSTH257
DDLDesdi_1431
DORDesor_1773
FWADCMF_01875 DCMF_04045 DCMF_26680
TJRTherJR_2412
ELMELI_3684
EMTCPZ25_018340
ELIMB2M23_08030
AWOAwo_c27890(odh)
AWICHL1_000715
TMRTmar_0905
THEFE1B22_08195
THEPDYI95_004515
LASAL9O85_05365
TTETTE0923
TEXTeth514_2108
THXThet_0827
TPDTeth39_1425
TITThit_0861
TMTTmath_0903
TBOThebr_1460
TWIThewi_1012
TKITKV_c09360(odh)
GEKkuro4_08130 kuro4_26110 kuro4_28280
TTMTthe_0328
TTOThethe_00276
TXYThexy_0233
TSHTsac_0905
MASMahau_2054
TEPTepRe1_0361
TAETepiRe1_0401(odh)
TOCToce_0231
HORHore_09740
HALSD7D81_11685
IFNGM661_09140
AARAcear_0924
AFTBBF96_06005
KMEH0A61_00159(odh_1) H0A61_00204(odh_2)
PEDING2D1G_0149 ING2D1G_1090 ING2D1G_1417
SPOAEQM13_13910
KPARJL105_06980 JL105_09105
SEDZJYG23_08225
MEDMELS_0825
DHODia5BBH33_20930
STEDSPTER_03930(odh)
AINAcin_2030
ERHERH_0138
ERSK210_07830
ERIEEI45_00915
LPILLIP_3237
NFANFA_20460
NFRERS450000_02063(odh)
NBRO3I_021570
GORKTR9_4000
GOQACH46_07700
GTABCM27_21020
GOMD7316_04418(odh)
GJIH1R19_19380
GHNMVF96_19865
SRCM271_41285
SBATG4Z16_16955
MIOAOA12_14890
ARZAUT26_08575
ARXARZXY2_1768
BCVBcav_1678
MPHMLP_28670(odh)
BRTJ4N02_07430 J4N02_09890
NAQUENKNEFLB_02180(odh)
SENSACE_4763
AMYCCU254_11050
PHHAFB00_16850
AIHAiant_66630
BADBAD_1262
AEQAEQU_0679
AHATADCFC_16820
ERZER308_13060
MPROBJP34_27140
CEPCri9333_0758
GLJGKIL_0005
RRSRoseRS_2460
RCARcas_2335
ATMANT_01050
ABATCFX1CAM_2187
PSUBPelsub_P0602
CAPCLDAP_31070
AMUAmuc_1748
RMLFF011L_11710(odh)
SASSMUG09_01520
EMIEmin_0881
FULC4N20_04580
STRSterm_0082
ACOAmico_1250 Amico_1803
TLITlie_1504 Tlie_1506 Tlie_1711
AMOAnamo_1614
PPIOCE91St28_22620 CE91St28_22770
BCAEA4V03_09980
PDIBDI_0433
PRUPRU_0088
MOAI6I89_09315
TLETlet_0900
TTATheth_1509
PHYAJ81_03765
TMETmel_0083
TAFTHA_300
TFEJYK00_09175
FPEFerpe_0740
PMOPmob_0984
DTNDTL3_0117
KOLKole_0924
KPFIX53_06555
MPGTheba_0163 Theba_0170
MINFMESINF_0945
ALAMRT761_00450(odh)
BARCAOA65_0109 AOA65_0338
LOKILokiarch_08200(odh)
PSYTDSAG12_00408
 » show all
Reference
1  [PMID:2753861]
  Authors
Asano Y, Yamaguchi K, Kondo K.
  Title
A new NAD+-dependent opine dehydrogenase from Arthrobacter sp. strain 1C.
  Journal
J Bacteriol 171:4466-71 (1989)
DOI:10.1128/JB.171.8.4466-4471.1989
Reference
2  [PMID:7487048]
  Authors
Dairi T, Asano Y
  Title
Cloning, nucleotide sequencing, and expression of an opine dehydrogenase gene from Arthrobacter sp. strain 1C.
  Journal
Appl Environ Microbiol 61:3169-71 (1995)
DOI:10.1128/AEM.61.8.3169-3171.1995
  Sequence
Reference
3
  Authors
Kato, Y., Yamada, H. and Asano, Y.
  Title
Stereoselective synthesis of opine-type secondary amine carboxylic acids by a new enzyme opine dehydrogenase. Use of recombinant enzymes.
  Journal
J Mol Catal, B Enzym 1:151-160 (1996)
Other DBs
ExplorEnz - The Enzyme Database: 1.5.1.28
IUBMB Enzyme Nomenclature: 1.5.1.28
ExPASy - ENZYME nomenclature database: 1.5.1.28
BRENDA, the Enzyme Database: 1.5.1.28
CAS: 108281-02-3

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