KEGG   ENZYME: 1.5.3.23Help
Entry
EC 1.5.3.23                 Enzyme                                 

Name
glyphosate oxidoreductase;
gox (gene name)
Class
Oxidoreductases;
Acting on the CH-NH group of donors;
With oxygen as acceptor
BRITE hierarchy
Sysname
glyphosate oxidoreductase (aminomethylphosphonate-forming)
Reaction(IUBMB)
2 glyphosate + O2 = 2 aminomethylphosphonate + 2 glyoxylate [RN:R11486]
Reaction(KEGG)
Substrate
glyphosate [CPD:C01705];
O2 [CPD:C00007]
Product
aminomethylphosphonate [CPD:C11033];
glyoxylate [CPD:C00048]
Comment
The enzyme, characterized from the bacterium Ochrobactrum sp. G-1, contains an FAD cofactor. The catalytic cycle starts with a reduction of the FAD cofactor by one molecule of glyphosate, yielding reduced FAD and a Schiff base of aminomethylphosphonate with glyoxylate that is hydrolysed to the single components. The reduced FAD is reoxidized by oxygen, generating water and an oxygenated flavin intermediate, which catalyses the oxygenation of a second molecule of glyphosate, forming the second pair of aminomethylphosphonate and glyoxylate.
History
EC 1.5.3.23 created 2016
Reference
1
  Authors
Barry, G. F. and Kishore. G. M.
  Title
Glyphosate tolerant plants.
  Journal
US patent US5463175 A (1995)
Reference
2  [PMID:21789492]
  Authors
Sviridov AV, Shushkova TV, Zelenkova NF, Vinokurova NG, Morgunov IG, Ermakova IT, Leontievsky AA
  Title
Distribution of glyphosate and methylphosphonate catabolism systems in soil bacteria Ochrobactrum anthropi and Achromobacter sp.
  Journal
Appl Microbiol Biotechnol 93:787-96 (2012)
DOI:10.1007/s00253-011-3485-y
Other DBs
ExplorEnz - The Enzyme Database: 1.5.3.23
IUBMB Enzyme Nomenclature: 1.5.3.23
ExPASy - ENZYME nomenclature database: 1.5.3.23
BRENDA, the Enzyme Database: 1.5.3.23

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