nctB (gene name)
Acting on the CH-NH group of donors;
With oxygen as acceptor
(S)-6-hydroxynicotine + H2O + O2 = 1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one + H2O2 (overall reaction) [RN:
(1a) (S)-6-hydroxynicotine + O2 = 5-(N-methyl-4,5-dihydro-1H-pyrrol-2-yl)pyridin-2-ol + H2O2 [RN:
(1b) 5-(N-methyl-4,5-dihydro-1H-pyrrol-2-yl)pyridin-2-ol + H2O = 1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one (spontaneous) [RN:
A flavoprotein (FAD). The enzyme, which participates in nicotine degradation, is specific for the (S) isomer of 6-hydroxynicotine. The bacterium Arthrobacter nicotinovorans, in which this enzyme was originally discovered, has a different enzyme that catalyses a similar reaction with the less common (R)-isomer (cf. EC
, (R)-6-hydroxynicotine oxidase).
EC 126.96.36.199 created 1972, modified 2015
ec00760 Nicotinate and nicotinamide metabolism
ec01120 Microbial metabolism in diverse environments
K19826 (S)-6-hydroxynicotine oxidase
Decker K, Bleeg H.
Induction and purification of stereospecific nicotine oxidizing enzymes from Arthrobacter oxidans.
Dai VD, Decker K, Sund H.
Purification and properties of L-6-hydroxynicotine oxidase.
Schenk S, Hoelz A, Krauss B, Decker K
Gene structures and properties of enzymes of the plasmid-encoded nicotine catabolism of Arthrobacter nicotinovorans.
Qiu J, Wei Y, Ma Y, Wen R, Wen Y, Liu W
A novel (S)-6-hydroxynicotine oxidase gene from Shinella sp. strain HZN7.
ExplorEnz - The Enzyme Database: 188.8.131.52
ExPASy - ENZYME nomenclature database: 184.108.40.206
UM-BBD (Biocatalysis/Biodegradation Database): 220.127.116.11
BRENDA, the Enzyme Database: 18.104.22.168