Entry
Name
pyrimidodiazepine synthase;
PDA synthase;
pyrimidodiazepine:oxidized-glutathione oxidoreductase (ring-opening, cyclizing);
pyrimidodiazepine:glutathione-disulfide oxidoreductase (ring-opening, cyclizing)
Class
Oxidoreductases;
Acting on the CH-NH group of donors;
With a disulfide as acceptor
BRITE hierarchy
Sysname
2-amino-6-acetyl-3,7,8,9-tetrahydro-3H-pyrimido[4,5-b][1,4]diazepin-4-one:glutathione-disulfide oxidoreductase (ring-opening, cyclizing)
Reaction(IUBMB)
2-amino-6-acetyl-3,7,8,9-tetrahydro-3H-pyrimido[4,5-b][1,4]diazepin-4-one + glutathione disulfide + H2O = 6-pyruvoyltetrahydropterin + 2 glutathione [RN:
R03984 ]
Reaction(KEGG)
Substrate
2-amino-6-acetyl-3,7,8,9-tetrahydro-3H-pyrimido[4,5-b][1,4]diazepin-4-one [CPD:
C02587 ];
glutathione disulfide [CPD:
C00127 ];
H2O [CPD:
C00001 ]
Product
6-pyruvoyltetrahydropterin [CPD:
C03684 ];
glutathione [CPD:
C00051 ]
Comment
In the reverse direction, the reduction of 6-pyruvoyl-tetrahydropterin is accompanied by the opening of the 6-membered pyrazine ring and the formation of the 7-membered diazepine ring. The pyrimidodiazepine formed is an acetyldihydro derivative. Involved in the formation of the eye pigment drosopterin in Drosophila melanogaster.
History
EC 1.5.4.1 created 1990, modified 2014
Pathway
Orthology
K00310 pyrimidodiazepine synthase
Genes
BFO : 118417884 118418016 118418017
APLC : 110980055 110980113
SKO : 102801608 102805287 102805394
DME : Dmel_CG6673(GstO2) Dmel_CG6776(GstO3) Dmel_CG6781(se)
DER : 6545776 6545777 6545778
DSI : Dsimw501_GD12980(Dsim_GD12980) Dsimw501_GD14099(Dsim_GD14099) Dsimw501_GD14100(Dsim_GD14100)
DYA : Dyak_GE20748 Dyak_GE20749 Dyak_GE21298
DSR : 110189883 110189884 110190145
DPO : 4813077 6900705 6900706
DPE : 6601434 6601435 6601436
DMN : 108151105 108151107 108151108
DWI : 6645944 6645945 6645946
DGR : 6558456 6558466 6558470
DMO : Dmoj_GI11973 Dmoj_GI11974 Dmoj_GI13342
DAZ : 108613357 108613358 108613359
DNV : 108650771 108650772 108650773
DHE : 111601723 111601724 111601725
AGA : AgaP_AGAP005749(GSTO1)
AALB : 109410292 109426263
CNS : 116336677 116336678 116344085 116345203
BAFF : 126913925 126915750
MGEN : 117221474 117221703
CGLO : 123266973 123266974
ATD : 109594047 109594062 109600220
AGB : 108909339 108909342 108915256
LDC : 111501821 111503060 111507790
PPYR : 116174034 116179350 116180517 116181187
BMOR : 100141441(GSTo4) 692819(GSTo1) 692941(GSTo2) 732973(GSTo3)
BMAN : 114246189 114251041 114251046 114251054
MSEX : 115440015 115440017 115440018 115450765
DPL : KGM_213590 KGM_213591 KGM_215101
BANY : 112045709 112052091 112052101 112052216
MJU : 123868217 123868218 123868219 123878841
VCD : 124536245 124536413 124541532
MCIX : 123659506 123659928 123666863
PXU : 106115870 106121024 106121486
PRAP : 110991775 110991776 110993706
ZCE : 119828334 119839062 119840698 119840898 119840913
LSIN : 126972490 126978185
HAW : 110380331 110380334 110382088
SLIU : 111351376 111358895 111359043
PXY : 105385065 105385083 105391501 105393538 105393990 105393991 105393992 105398022
PGW : 126372314 126372316 126378972
HHAL : 106679272 106679413
ZNE : 110829188 110841526 110841528
CSEC : 111861574 111861586 111868728
SGRE : 126269683 126305734
FCD : 110846827 110848295 110848529 110860205
PCHN : 125037357 125037499
HAME : 121872925 121878606
ISC : IscW_ISCW003939 IscW_ISCW006477
CSCU : 111617892 111617895 111634163
PMEO : 129585152 129585304 129586526 129586557 129601368 129601635
TAD : TRIADDRAFT_16556 TRIADDRAFT_21044 TRIADDRAFT_33986 TRIADDRAFT_34018 TRIADDRAFT_62355 TRIADDRAFT_62357
» show all
Taxonomy
Reference
Authors
Wiederrecht GJ, Brown GM.
Title
Purification and properties of the enzymes from Drosophila melanogaster that catalyze the conversion of dihydroneopterin triphosphate to the pyrimidodiazepine precursor of the drosopterins.
Journal
J Biol Chem 259:14121-7 (1984)
Reference
Authors
Kim J, Suh H, Kim S, Kim K, Ahn C, Yim J
Title
Identification and characteristics of the structural gene for the Drosophila eye colour mutant sepia, encoding PDA synthase, a member of the omega class glutathione S-transferases.
Journal
Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.5.4.1
ExPASy - ENZYME nomenclature database: 1.5.4.1
BRENDA, the Enzyme Database: 1.5.4.1