KEGG   ENZYME: 1.5.4.1
Entry
EC 1.5.4.1                  Enzyme                                 
Name
pyrimidodiazepine synthase;
PDA synthase;
pyrimidodiazepine:oxidized-glutathione oxidoreductase (ring-opening, cyclizing);
pyrimidodiazepine:glutathione-disulfide oxidoreductase (ring-opening, cyclizing)
Class
Oxidoreductases;
Acting on the CH-NH group of donors;
With a disulfide as acceptor
Sysname
2-amino-6-acetyl-3,7,8,9-tetrahydro-3H-pyrimido[4,5-b][1,4]diazepin-4-one:glutathione-disulfide oxidoreductase (ring-opening, cyclizing)
Reaction(IUBMB)
2-amino-6-acetyl-3,7,8,9-tetrahydro-3H-pyrimido[4,5-b][1,4]diazepin-4-one + glutathione disulfide + H2O = 6-pyruvoyltetrahydropterin + 2 glutathione [RN:R03984]
Reaction(KEGG)
R03984
Substrate
2-amino-6-acetyl-3,7,8,9-tetrahydro-3H-pyrimido[4,5-b][1,4]diazepin-4-one [CPD:C02587];
glutathione disulfide [CPD:C00127];
H2O [CPD:C00001]
Product
6-pyruvoyltetrahydropterin [CPD:C03684];
glutathione [CPD:C00051]
Comment
In the reverse direction, the reduction of 6-pyruvoyl-tetrahydropterin is accompanied by the opening of the 6-membered pyrazine ring and the formation of the 7-membered diazepine ring. The pyrimidodiazepine formed is an acetyldihydro derivative. Involved in the formation of the eye pigment drosopterin in Drosophila melanogaster.
History
EC 1.5.4.1 created 1990, modified 2014
Pathway
ec00480  Glutathione metabolism
ec00790  Folate biosynthesis
ec01100  Metabolic pathways
Orthology
K00310  pyrimidodiazepine synthase
Genes
DMEDmel_CG6781(se)
DER6545777
DSE6604890
DSIDsimw501_GD14100(Dsim_GD14100)
DYADyak_GE20749
DAN6506964
DSR110189884
DPO4813077
DPE6601435
DMN108151105
DWI6645945
DMODmoj_GI11974
DAZ108613358
DNV108650772
DHE111601724
DVI6624546(se)
 » show all
Reference
1  [PMID:6438092]
  Authors
Wiederrecht GJ, Brown GM.
  Title
Purification and properties of the enzymes from Drosophila melanogaster that catalyze the conversion of dihydroneopterin triphosphate to the pyrimidodiazepine precursor of the drosopterins.
  Journal
J Biol Chem 259:14121-7 (1984)
Reference
2  [PMID:16712527]
  Authors
Kim J, Suh H, Kim S, Kim K, Ahn C, Yim J
  Title
Identification and characteristics of the structural gene for the Drosophila eye colour mutant sepia, encoding PDA synthase, a member of the omega class glutathione S-transferases.
  Journal
Biochem J 398:451-60 (2006)
DOI:10.1042/BJ20060424
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.5.4.1
IUBMB Enzyme Nomenclature: 1.5.4.1
ExPASy - ENZYME nomenclature database: 1.5.4.1
BRENDA, the Enzyme Database: 1.5.4.1
CAS: 93586-06-2

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