KEGG ENZYME: 1.5.4.1

ENZYME: 1.5.4.1

Entry

EC 1.5.4.1                  Enzyme

Name

pyrimidodiazepine synthase;
PDA synthase;
pyrimidodiazepine:oxidized-glutathione oxidoreductase (ring-opening, cyclizing);
pyrimidodiazepine:glutathione-disulfide oxidoreductase (ring-opening, cyclizing)

Class

Oxidoreductases;
Acting on the CH-NH group of donors;
With a disulfide as acceptor

Sysname

2-amino-6-acetyl-3,7,8,9-tetrahydro-3H-pyrimido[4,5-b][1,4]diazepin-4-one:glutathione-disulfide oxidoreductase (ring-opening, cyclizing)

Reaction(IUBMB)

2-amino-6-acetyl-3,7,8,9-tetrahydro-3H-pyrimido[4,5-b][1,4]diazepin-4-one + glutathione disulfide + H2O = 6-pyruvoyltetrahydropterin + 2 glutathione [RN:R03984]

Reaction(KEGG)

R03984

Substrate

2-amino-6-acetyl-3,7,8,9-tetrahydro-3H-pyrimido[4,5-b][1,4]diazepin-4-one [CPD:C02587];
glutathione disulfide [CPD:C00127];
H2O [CPD:C00001]

Product

6-pyruvoyltetrahydropterin [CPD:C03684];
glutathione [CPD:C00051]

Comment

In the reverse direction, the reduction of 6-pyruvoyl-tetrahydropterin is accompanied by the opening of the 6-membered pyrazine ring and the formation of the 7-membered diazepine ring. The pyrimidodiazepine formed is an acetyldihydro derivative. Involved in the formation of the eye pigment drosopterin in Drosophila melanogaster.

History

EC 1.5.4.1 created 1990, modified 2014

Pathway

ec00480

Glutathione metabolism

ec00790

Folate biosynthesis

ec01100

Metabolic pathways

Orthology

K00310

pyrimidodiazepine synthase

Genes

BFO:	118417884 118418016 118418017

SCLV:

120347446

APLC:

110980055 110980113

SKO:	102801608 102805287 102805394

DME:	Dmel_CG6673(GstO2) Dmel_CG6776(GstO3) Dmel_CG6781(se)

DER:	6545776 6545777 6545778

DSE:	6604889 6604890

DSI:	Dsimw501_GD12980(Dsim_GD12980) Dsimw501_GD14099(Dsim_GD14099) Dsimw501_GD14100(Dsim_GD14100)

DYA:	Dyak_GE20748 Dyak_GE20749 Dyak_GE21298

DAN:

6506964

DSR:	110189883 110189884 110190145

DPO:	Dpse_GA19859 Dpse_GA23449 Dpse_GA23844

DPE:	6601434 6601435 6601436

DMN:	108151105 108151107 108151108

DWI:	6645944 6645945 6645946

DGR:	6558456 6558466 6558470

DMO:	Dmoj_GI11973 Dmoj_GI11974 Dmoj_GI13342

DAZ:	108613357 108613358 108613359

DNV:	108650771 108650772 108650773

DHE:	111601723 111601724 111601725

DVI:	6622272 6624546

MDE:

101891696

AGA:	AgaP_AGAP005749(GSTO1)

ACOZ:

120953261

AARA:

120908851

AAG:

23687505

AALB:

109410292 109426263

CQU:	CpipJ_CPIJ000031

CPII:

120421894

CNS:	116336677 116336678 116344085 116345203

AME:

552118

ACER:

108001760

ALAB:

122716860

BIM:

100745560

BBIF:

117211745

BVK:

117236127

BVAN:

117153685

BTER:

100646742

BPYO:

122569507

CCAL:

108628071

OBB:

114882731

MGEN:

117221474 117221703

NMEA:

116424254

CGIG:

122395362

SOC:

105200535

AEC:

105147922

ACEP:

105622284

PBAR:

105424214

VEM:

105563796

HST:

105185767

DQU:

106745381

CFO:

105256274

FEX:

115237135

LHU:

105676415

PGC:

109858549

OBO:

105280891

PCF:

106789144

PFUC:

122518209

VPS:	122627925 122637254

NVI:	100116224(GstO1)

CSOL:

105363631

TPRE:

106652488

MDL:

103577336

CGLO:

123266973 123266974

FAS:

105273999

DAM:

107048478

CCIN:

107263414

TCA:	655757 659748

DPA:	109534346 109542472

SOY:	115877807 115880910

ATD:	109594047 109594062 109600220 109606227

AGB:	108909339 108909342 108915256

LDC:	111501821 111503060 111507790

NVL:

108564623

APLN:

108743348

PPYR:

116174034 116179350 116180517 116181187

OTU:	111417405 111421098

BMOR:

100141441(GSTo4) 692819(GSTo1) 692941(GSTo2) 732973(GSTo3)

BMAN:

114246189 114251041 114251046 114251054

MSEX:

115440015 115440017 115440018 115450765

DPL:	KGM_213590 KGM_213591 KGM_215101

BANY:

112045709 112052091 112052101 112052216

PMAC:

106708065

PPOT:

106107676

PXU:	106115870 106121024 106121486

PRAP:

110991775 110991776 110993706

ZCE:	119828334 119839062 119840698 119840898 119840913

HAW:	110380331 110380334 110382088

TNL:	113501099 113505714

SLIU:

111351376 111358895 111359043

OFU:	114358125 114363188

PXY:	105385065 105385083 105391501 105393538 105393990 105393991 105393992 105398022

API:	100167548 103310566

DNX:

107163884

AGS:

114121972

RMD:

113551979

BTAB:

109042194

DCI:	103512934 103519641

CLEC:

106668275

HHAL:

106679272 106679413

NLU:

111048776

PHU:	Phum_PHUM530530

FOC:

113209890

ZNE:	110829188 110841526 110841528

CSEC:

111861574 111861586 111868728

FCD:	110846827 110848295 110848529 110860205

DPX:	DAPPUDRAFT_318250

PVM:	113808340 113818669

PJA:

122256350

PCHN:

125037357 125037499

HAME:

121872925 121878606

PCLA:

123768218

ISC:	IscW_ISCW003939 IscW_ISCW006477

RSAN:

119396330

RMP:

119174552

VDE:

111247779

VJA:

111261323

TUT:

107364114

CSCU:

111617892 111617895 111634163

HRO:	HELRODRAFT_180211

MMER:

123548299

LAK:	106159330 106162221

SMM:	Smp_152710.1

OVI:	T265_01660

TAD:	TRIADDRAFT_16556 TRIADDRAFT_21044 TRIADDRAFT_33986 TRIADDRAFT_34018 TRIADDRAFT_62355 TRIADDRAFT_62357

E1B28_005236

» show all

Reference

1 [PMID:6438092]

Authors

Wiederrecht GJ, Brown GM.

Title

Purification and properties of the enzymes from Drosophila melanogaster that catalyze the conversion of dihydroneopterin triphosphate to the pyrimidodiazepine precursor of the drosopterins.

Journal

J Biol Chem 259:14121-7 (1984)

Reference

2 [PMID:16712527]

Authors

Kim J, Suh H, Kim S, Kim K, Ahn C, Yim J

Title

Identification and characteristics of the structural gene for the Drosophila eye colour mutant sepia, encoding PDA synthase, a member of the omega class glutathione S-transferases.

Journal

Biochem J 398:451-60 (2006)
DOI:10.1042/BJ20060424

Sequence

[dme:Dmel_CG6781]

Other DBs

ExplorEnz - The Enzyme Database:

1.5.4.1

IUBMB Enzyme Nomenclature:

1.5.4.1

ExPASy - ENZYME nomenclature database:

1.5.4.1

BRENDA, the Enzyme Database:

1.5.4.1

CAS:	93586-06-2

DBGET integrated database retrieval system