KEGG   ENZYME: 1.5.4.1
Entry
EC 1.5.4.1                  Enzyme                                 
Name
pyrimidodiazepine synthase;
PDA synthase;
pyrimidodiazepine:oxidized-glutathione oxidoreductase (ring-opening, cyclizing);
pyrimidodiazepine:glutathione-disulfide oxidoreductase (ring-opening, cyclizing)
Class
Oxidoreductases;
Acting on the CH-NH group of donors;
With a disulfide as acceptor
Sysname
2-amino-6-acetyl-3,7,8,9-tetrahydro-3H-pyrimido[4,5-b][1,4]diazepin-4-one:glutathione-disulfide oxidoreductase (ring-opening, cyclizing)
Reaction(IUBMB)
2-amino-6-acetyl-3,7,8,9-tetrahydro-3H-pyrimido[4,5-b][1,4]diazepin-4-one + glutathione disulfide + H2O = 6-pyruvoyltetrahydropterin + 2 glutathione [RN:R03984]
Reaction(KEGG)
R03984
Substrate
2-amino-6-acetyl-3,7,8,9-tetrahydro-3H-pyrimido[4,5-b][1,4]diazepin-4-one [CPD:C02587];
glutathione disulfide [CPD:C00127];
H2O [CPD:C00001]
Product
6-pyruvoyltetrahydropterin [CPD:C03684];
glutathione [CPD:C00051]
Comment
In the reverse direction, the reduction of 6-pyruvoyl-tetrahydropterin is accompanied by the opening of the 6-membered pyrazine ring and the formation of the 7-membered diazepine ring. The pyrimidodiazepine formed is an acetyldihydro derivative. Involved in the formation of the eye pigment drosopterin in Drosophila melanogaster.
History
EC 1.5.4.1 created 1990, modified 2014
Pathway
ec00480  Glutathione metabolism
ec00790  Folate biosynthesis
ec01100  Metabolic pathways
Orthology
K00310  pyrimidodiazepine synthase
Genes
BFO118417884 118418016 118418017
SCLV120347446
APLC110980055 110980113
SKO102801608 102805287 102805394
DMEDmel_CG6673(GstO2) Dmel_CG6776(GstO3) Dmel_CG6781(se)
DER6545776 6545777 6545778
DSE6604889 6604890
DSIDsimw501_GD12980(Dsim_GD12980) Dsimw501_GD14099(Dsim_GD14099) Dsimw501_GD14100(Dsim_GD14100)
DYADyak_GE20748 Dyak_GE20749 Dyak_GE21298
DAN6506964
DSR110189883 110189884 110190145
DPODpse_GA19859 Dpse_GA23449 Dpse_GA23844
DPE6601434 6601435 6601436
DMN108151105 108151107 108151108
DWI6645944 6645945 6645946
DGR6558456 6558466 6558470
DMODmoj_GI11973 Dmoj_GI11974 Dmoj_GI13342
DAZ108613357 108613358 108613359
DNV108650771 108650772 108650773
DHE111601723 111601724 111601725
DVI6622272 6624546
MDE101891696
AGAAgaP_AGAP005749(GSTO1)
ACOZ120953261
AARA120908851
AAG23687505
AALB109410292 109426263
CQUCpipJ_CPIJ000031
CPII120421894
CNS116336677 116336678 116344085 116345203
AME552118
ACER108001760
ALAB122716860
BIM100745560
BBIF117211745
BVK117236127
BVAN117153685
BTER100646742
BPYO122569507
CCAL108628071
OBB114882731
MGEN117221474 117221703
NMEA116424254
CGIG122395362
SOC105200535
AEC105147922
ACEP105622284
PBAR105424214
VEM105563796
HST105185767
DQU106745381
CFO105256274
FEX115237135
LHU105676415
PGC109858549
OBO105280891
PCF106789144
PFUC122518209
VPS122627925 122637254
NVI100116224(GstO1)
CSOL105363631
TPRE106652488
MDL103577336
CGLO123266973 123266974
FAS105273999
DAM107048478
CCIN107263414
TCA655757 659748
DPA109534346 109542472
SOY115877807 115880910
ATD109594047 109594062 109600220 109606227
AGB108909339 108909342 108915256
LDC111501821 111503060 111507790
NVL108564623
APLN108743348
PPYR116174034 116179350 116180517 116181187
OTU111417405 111421098
BMOR100141441(GSTo4) 692819(GSTo1) 692941(GSTo2) 732973(GSTo3)
BMAN114246189 114251041 114251046 114251054
MSEX115440015 115440017 115440018 115450765
DPLKGM_213590 KGM_213591 KGM_215101
BANY112045709 112052091 112052101 112052216
PMAC106708065
PPOT106107676
PXU106115870 106121024 106121486
PRAP110991775 110991776 110993706
ZCE119828334 119839062 119840698 119840898 119840913
HAW110380331 110380334 110382088
TNL113501099 113505714
SLIU111351376 111358895 111359043
OFU114358125 114363188
PXY105385065 105385083 105391501 105393538 105393990 105393991 105393992 105398022
API100167548 103310566
DNX107163884
AGS114121972
RMD113551979
BTAB109042194
DCI103512934 103519641
CLEC106668275
HHAL106679272 106679413
NLU111048776
PHUPhum_PHUM530530
FOC113209890
ZNE110829188 110841526 110841528
CSEC111861574 111861586 111868728
FCD110846827 110848295 110848529 110860205
DPXDAPPUDRAFT_318250
PVM113808340 113818669
PJA122256350
PCHN125037357 125037499
HAME121872925 121878606
PCLA123768218
ISCIscW_ISCW003939 IscW_ISCW006477
RSAN119396330
RMP119174552
VDE111247779
VJA111261323
TUT107364114
CSCU111617892 111617895 111634163
HROHELRODRAFT_180211
MMER123548299
LAK106159330 106162221
SMMSmp_152710.1
OVIT265_01660
TADTRIADDRAFT_16556 TRIADDRAFT_21044 TRIADDRAFT_33986 TRIADDRAFT_34018 TRIADDRAFT_62355 TRIADDRAFT_62357
MOREE1B28_005236
 » show all
Reference
1  [PMID:6438092]
  Authors
Wiederrecht GJ, Brown GM.
  Title
Purification and properties of the enzymes from Drosophila melanogaster that catalyze the conversion of dihydroneopterin triphosphate to the pyrimidodiazepine precursor of the drosopterins.
  Journal
J Biol Chem 259:14121-7 (1984)
Reference
2  [PMID:16712527]
  Authors
Kim J, Suh H, Kim S, Kim K, Ahn C, Yim J
  Title
Identification and characteristics of the structural gene for the Drosophila eye colour mutant sepia, encoding PDA synthase, a member of the omega class glutathione S-transferases.
  Journal
Biochem J 398:451-60 (2006)
DOI:10.1042/BJ20060424
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.5.4.1
IUBMB Enzyme Nomenclature: 1.5.4.1
ExPASy - ENZYME nomenclature database: 1.5.4.1
BRENDA, the Enzyme Database: 1.5.4.1
CAS: 93586-06-2

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