KEGG   ENZYME: 1.5.99.12Help
Entry
EC 1.5.99.12                Enzyme                                 

Name
cytokinin dehydrogenase;
N6-dimethylallyladenine:(acceptor) oxidoreductase;
6-N-dimethylallyladenine:acceptor oxidoreductase;
OsCKX2;
CKX;
cytokinin oxidase/dehydrogenase
Class
Oxidoreductases;
Acting on the CH-NH group of donors;
With unknown physiological acceptors
BRITE hierarchy
Sysname
N6-dimethylallyladenine:acceptor oxidoreductase
Reaction(IUBMB)
N6-dimethylallyladenine + acceptor + H2O = adenine + 3-methylbut-2-enal + reduced acceptor [RN:R05708]
Reaction(KEGG)
Substrate
N6-dimethylallyladenine [CPD:C04083];
acceptor [CPD:C00028];
H2O [CPD:C00001]
Product
adenine [CPD:C00147];
3-methylbut-2-enal [CPD:C07330];
reduced acceptor [CPD:C00030]
Comment
A flavoprotein(FAD). Catalyses the oxidation of cytokinins, a family of N6-substituted adenine derivatives that are plant hormones, where the substituent is a dimethylallyl or other prenyl group. Although this activity was previously thought to be catalysed by a hydrogen-peroxide-forming oxidase, this enzyme does not require oxygen for activity and does not form hydrogen peroxide. 2,6-Dichloroindophenol, methylene blue, nitroblue tetrazolium, phenazine methosulfate and Cu(II) in the presence of imidazole can act as acceptors. This enzyme plays a part in regulating rice-grain production, with lower levels of the enzyme resulting in enhanced grain production [2].
History
EC 1.5.99.12 created 2001
Pathway
ec00908  Zeatin biosynthesis
Orthology
K00279  cytokinin dehydrogenase
Genes
ATH: AT1G75450(CKX5) AT2G19500(CKX2) AT2G41510(CKX1) AT3G63440(CKX6) AT4G29740(CKX4) AT5G21482(CKX7) AT5G56970(CKX3)
ALY: ARALYDRAFT_476695 ARALYDRAFT_480522 ARALYDRAFT_483228 ARALYDRAFT_489034 ARALYDRAFT_657086 ARALYDRAFT_667269 ARALYDRAFT_918856
CRB: 17877017 17884533 17885237 17887842 17890111 17892323 17895153
CSAT: 104701434 104702290 104704111 104706475 104713195 104717225 104721893 104726241 104730350 104733218 104733803 104736200 104747884 104748975 104751417 104763538 104770512 104782642 104785229 104789862 104793063 109124994
EUS: EUTSA_v10005878mg EUTSA_v10013259mg EUTSA_v10013271mg EUTSA_v10016470mg EUTSA_v10018378mg EUTSA_v10023158mg EUTSA_v10024918mg
BRP: 103249144(CKX2) 103249171(CKX3) 103831996 103842132 103845129 103845596(CKX) 103849258 103851408 103857986(CKX1) 103861901 103865890 103866566(BrCKX1)
BNA: 106354495 106356750 106365552 106370330 106371682 106374136 106374280 106384861 106395144 106396215 106403221 106405957 106406332 106406751 106409476 106409493 106415584 106415860 106417025 106417645 106421388 106427274 106436345 106437984 106450132 106450297 106450662 111198549 111213862
BOE: 106300565 106302335 106304349 106307242 106314001 106316377 106319493 106324800 106327746 106328016 106340592 106340923
THJ: 104799579 104801962 104801969 104809069 104810957 104813263 104814623 104819113
CPAP: 110807147 110808166 110809263 110810796 110817106
LJA: Lj0g3v0360459.1(Lj0g3v0360459.1) Lj1g3v4226830.1(Lj1g3v4226830.1) Lj3g3v0809870.1(Lj3g3v0809870.1) Lj3g3v3650360.1(Lj3g3v3650360.1) Lj4g3v2171460.1(Lj4g3v2171460.1) Lj4g3v2641050.1(Lj4g3v2641050.1) Lj5g3v0434840.1(Lj5g3v0434840.1) Lj5g3v0692300.1(Lj5g3v0692300.1) Lj5g3v2238680.1(Lj5g3v2238680.1) Lj6g3v1249630.1(Lj6g3v1249630.1)
SLY: 100874535(SlCKX5) 100874536(SlCKX7) 100874537(SlCKX2) 101055579(SlCKX6) 101055598(SlCKX1) 101055612(SlCKX4) 101244307(SlCKX3) 101255039(SlCKX8) 101266491
DOSA: Os01t0187600-01(Os01g0187600) Os01t0197700-01(Os01g0197700) Os01t0775400-01(Os01g0775400) Os01t0940000-01(Os01g0940000) Os02t0220000-00(Os02g0220000) Os02t0220100-00(Os02g0220100) Os04t0523500-00(Os04g0523500) Os05t0374200-00(Os05g0374200) Os06t0572300-00(Os06g0572300) Os10t0483500-01(Os10g0483500)
ATS: 109733489(LOC109733489) 109736725(LOC109736725) 109737583(LOC109737583) 109739344(LOC109739344) 109740122(LOC109740122) 109751588(LOC109751588) 109751596(LOC109751596) 109755232(LOC109755232) 109756036(LOC109756036) 109757480(LOC109757480) 109759733(LOC109759733) 109759953(LOC109759953) 109769177(LOC109769177) 109772148(LOC109772148) 109775285(LOC109775285) 109775286(LOC109775286) 109784599(LOC109784599)
ZMA: 100272805(si660055c01) 100280446(CKX10) 100526747(CKX8) 100526749(CKX5) 100526750(CKX6) 100526752(CKX4b) 100526753(CKX12) 103640050 103641859 103654801 542056(cko3) 542507(cko2) 542585(cko1)
ANA: all0324
AVA: Ava_4717
 » show all
Taxonomy
Reference
1  [PMID:11168382]
  Authors
Galuszka P, Frebort I, Sebela M, Sauer P, Jacobsen S, Pec P.
  Title
Cytokinin oxidase or dehydrogenase? Mechanism of cytokinin degradation in cereals.
  Journal
Eur J Biochem 268:450-61 (2001)
DOI:10.1046/j.1432-1033.2001.01910.x
Reference
2  [PMID:15976269]
  Authors
Ashikari M, Sakakibara H, Lin S, Yamamoto T, Takashi T, Nishimura A, Angeles ER, Qian Q, Kitano H, Matsuoka M
  Title
Cytokinin oxidase regulates rice grain production.
  Journal
Science 309:741-5 (2005)
DOI:10.1126/science.1113373
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.5.99.12
IUBMB Enzyme Nomenclature: 1.5.99.12
ExPASy - ENZYME nomenclature database: 1.5.99.12
BRENDA, the Enzyme Database: 1.5.99.12
CAS: 55326-39-1

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