KEGG   ENZYME: 1.6.3.1
Entry
EC 1.6.3.1                  Enzyme                                 

Name
NAD(P)H oxidase (H2O2-forming);
THOX2;
ThOX;
dual oxidase;
p138tox;
thyroid NADPH oxidase;
thyroid oxidase;
thyroid oxidase 2;
NADPH oxidase;
NAD(P)H:oxygen oxidoreductase;
NAD(P)H oxidase
Class
Oxidoreductases;
Acting on NADH or NADPH;
With oxygen as acceptor
Sysname
NAD(P)H:oxygen oxidoreductase (H2O2-forming)
Reaction(IUBMB)
NAD(P)H + H+ + O2 = NAD(P)+ + H2O2 [RN:R07171 R07172]
Reaction(KEGG)
R07171 R07172
Substrate
NADH [CPD:C00004];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
NAD+ [CPD:C00003];
NADP+ [CPD:C00006];
H2O2 [CPD:C00027]
Comment
Requires FAD, heme and calcium. When calcium is present, this transmembrane glycoprotein generates H2O2 by transfering electrons from intracellular NAD(P)H to extracellular molecular oxygen. The electron bridge within the enzyme contains one molecule of FAD and probably two heme groups. This flavoprotein is expressed at the apical membrane of thyrocytes, and provides H2O2 for the thyroid peroxidase-catalysed biosynthesis of thyroid hormones.
History
EC 1.6.3.1 created 2003, modified 2013
Orthology
K13411  dual oxidase
Genes
HSA: 50506(DUOX2) 53905(DUOX1)
PTR: 453395(DUOX2) 467673(DUOX1)
PPS: 100974723(DUOX1) 100978429(DUOX2)
GGO: 101137548(DUOX1) 101139037(DUOX2)
PON: 100445893(DUOX2) 100447745(DUOX1)
NLE: 100588618(DUOX1) 100595406(DUOX2)
MCC: 713126(DUOX1) 713906(DUOX2)
MCF: 102131806(DUOX2) 102133834(DUOX1)
CSAB: 103245704(DUOX2) 103245708(DUOX1)
RRO: 104653891(DUOX2) 104653893(DUOX1)
RBB: 108530131(DUOX1) 108530134(DUOX2)
CJC: 100387872(DUOX2) 100406741(DUOX1)
SBQ: 101043225(DUOX1) 101045049(DUOX2)
MMU: 214593(Duox2) 99439(Duox1)
MCAL: 110289528(Duox2) 110289597(Duox1)
MPAH: 110318871(Duox2) 110318947(Duox1)
RNO: 266807(Duox1) 79107(Duox2)
MUN: 110553262(Duox2) 110553266(Duox1)
CGE: 100764526(Duox2) 100774391(Duox1)
NGI: 103746898(Duox2) 103746936(Duox1)
HGL: 101698133(Duox1) 101698889(Duox2)
CCAN: 109692712(Duox2) 109692713(Duox1)
OCU: 100344845(DUOX1) 100345610(DUOX2)
TUP: 102471164(DUOX2) 102496442(DUOX1)
CFA: 403719(DUOX2) 403720(DUOX1)
VVP: 112919876(DUOX2) 112919877(DUOX1)
AML: 100469477
UMR: 103656587(DUOX2) 103656588(DUOX1)
ORO: 101365122(DUOX2) 101365433(DUOX1)
FCA: 101087408(DUOX2) 101091374(DUOX1)
PTG: 102968075(DUOX2) 102968369(DUOX1)
PPAD: 109263047(DUOX1) 109263048(DUOX2)
AJU: 106986276(DUOX1) 106986277
BTA: 510409(DUOX1) 785698(DUOX2)
BOM: 102272102(DUOX2) 102277170(DUOX1)
BIU: 109564555(DUOX1) 109565168(DUOX2)
BBUB: 102403106(DUOX1) 102405152(DUOX2)
CHX: 102179569(DUOX1) 106502554(DUOX2)
OAS: 100462743(DUOX2) 101111972(DUOX1)
SSC: 397060(DUOX2) 397177(DUOX1)
CFR: 102510558(DUOX1) 102510795(DUOX2)
CDK: 105101748(DUOX1) 105101749(DUOX2)
BACU: 103001055(DUOX2) 103001339(DUOX1)
LVE: 103071982(DUOX2) 103076766(DUOX1)
PCAD: 102980878(DUOX2) 102993288(DUOX1)
ECB: 100070541(DUOX1) 100070566(DUOX2)
EPZ: 103554035(DUOX1) 103554037(DUOX2)
EAI: 106834993(DUOX1) 106834997(DUOX2)
MYB: 102242003(DUOX2) 102252627(DUOX1)
MYD: 102771512(DUOX1) 102771797(DUOX2)
MNA: 107536153(DUOX1) 107536249(DUOX2)
HAI: 109396658(DUOX1) 109396662
DRO: 112311051(DUOX2) 112311507(DUOX1)
PALE: 102894515(DUOX1) 102895435(DUOX2)
RAY: 107514952(DUOX2) 107514968(DUOX1)
MJV: 108400250 108400257(DUOX1)
LAV: 100661484(DUOX2) 100677682(DUOX1)
MDO: 100012658(DUOX2) 100012836(DUOX1)
SHR: 100931267(DUOX2) 100932050(DUOX1)
PCW: 110223647(DUOX1) 110223721
OAA: 100089154(DUOX2) 100093104
GGA: 427482(DUOX2)
MGP: 100538948(DUOX2)
CJO: 107318475(DUOX2)
NMEL: 110403879(DUOX2)
APLA: 101800626(DUOX2)
ACYG: 106047419(DUOX2)
TGU: 100218721
LSR: 110483946
SCAN: 103816154
GFR: 102037063(DUOX2)
FAB: 101805677(DUOX2)
PHI: 102105296(DUOX2)
PMAJ: 107209583
CCAE: 111934317
CCW: 104697496
ETL: 114060481(DUOX2)
FPG: 101914675(DUOX2)
FCH: 102054788
CLV: 102097710(DUOX2)
EGZ: 104130687(DUOX2)
NNI: 104016775(DUOX2)
ACUN: 113483989
PADL: 103916812(DUOX2)
AAM: 106487968
ASN: 102372872
AMJ: 102573689(DUOX2)
PSS: 102461565(DUOX2)
CMY: 102933380(DUOX2)
CPIC: 101942492(DUOX2)
ACS: 100562649
PVT: 110091655
PBI: 103059551(DUOX2)
PMUR: 107294062(DUOX2)
PMUA: 114584565(DUOX2)
GJA: 107106710
XLA: 108710292(duox2.L) 108711340 108712328(duox2.S)
XTR: 100486050(duox2) 100486194(duox1)
DRE: 565097(duox)
CCAR: 109059806
IPU: 108275020(duox1)
PHYP: 113529691(duox1)
AMEX: 103026091(duox1)
EEE: 113584048
TRU: 100124426(duox)
LCO: 104937849(duox1)
NCC: 104960088(duox1)
MZE: 101470770
ONL: 100696790(duox1)
OLA: 101160477(duox1)
XMA: 102217295(duox1)
XCO: 114151766(duox1)
PRET: 103466440(duox1)
CVG: 107087399
NFU: 107388507
KMR: 108229956(duox1)
ALIM: 106526813(duox1)
AOCE: 111577711
CSEM: 103380468(duox1)
POV: 109624285(duox1)
LCF: 108876727(duox1)
SDU: 111229749(duox1)
SLAL: 111658523(duox1)
HCQ: 109507657(duox1)
BPEC: 110169429
MALB: 109973835
OTW: 112257697
ELS: 105018355(duox1)
PKI: 111835096(duox1)
LCM: 102354060
CIN: 100170036(duox-d) 100170037(duox-c) 100170038(duox-b) 100170039(duox-a)
SPU: 100893745 588656(duox1)
APLC: 110980995
DME: Dmel_CG3131(Duox)
DER: 6542143
DSE: 6613156
DSI: Dsimw501_GD22800(Dsim_GD22800)
DAN: 6497732
DSR: 110185672
DPE: 6603391
DMN: 108163035
DWI: 6644426
DAZ: 108610468
DNV: 115564315
DHE: 111592500
MDE: 101898456
LCQ: 111691057
AGA: AgaP_AGAP009978(DUOX)
AAG: 5569378
AME: 551970
BIM: 100742890
BTER: 100646100
CCAL: 108628573
OBB: 114871314
AEC: 105143353
ACEP: 105623067
NVI: 100123854
CSOL: 105360056
DPA: 109541703
BMOR: 101742234
BMAN: 114249899
PMAC: 106717948
PRAP: 110992372
HAW: 110372854
TNL: 113498966
PXY: 105389437
API: 100160850
DNX: 107163754
AGS: 114128657
RMD: 113556351
BTAB: 109040851
CLEC: 106669322
CEL: CELE_F53G12.3(duox-2) CELE_F56C11.1(bli-3)
CBR: CBG05103(Cbr-bli-3)
BMY: Bm1_45640
TSP: Tsp_00940
OBI: 106882005
AQU: 100637823
MGR: MGG_08299
 » show all
Reference
1  [PMID:12110737]
  Authors
Moreno JC, Bikker H, Kempers MJ, van Trotsenburg AS, Baas F, de Vijlder JJ, Vulsma T, Ris-Stalpers C.
  Title
Inactivating mutations in the gene for thyroid oxidase 2 (THOX2) and congenital hypothyroidism.
  Journal
N Engl J Med 347:95-102 (2002)
DOI:10.1056/NEJMoa012752
  Sequence
[hsa:50506]
Reference
2  [PMID:11822874]
  Authors
De Deken X, Wang D, Dumont JE, Miot F.
  Title
Characterization of ThOX proteins as components of the thyroid H(2)O(2)-generating system.
  Journal
Exp Cell Res 273:187-96 (2002)
DOI:10.1006/excr.2001.5444
  Sequence
[hsa:53905 50506]
Reference
3  [PMID:10806195]
  Authors
De Deken X, Wang D, Many MC, Costagliola S, Libert F, Vassart G, Dumont JE, Miot F.
  Title
Cloning of two human thyroid cDNAs encoding new members of the NADPH oxidase family.
  Journal
J Biol Chem 275:23227-33 (2000)
DOI:10.1074/jbc.M000916200
  Sequence
[hsa:53905 50506]
Reference
4  [PMID:10601291]
  Authors
Dupuy C, Ohayon R, Valent A, Noel-Hudson MS, Deme D, Virion A.
  Title
Purification of a novel flavoprotein involved in the thyroid NADPH oxidase. Cloning of the porcine and human cdnas.
  Journal
J Biol Chem 274:37265-9 (1999)
DOI:10.1074/jbc.274.52.37265
  Sequence
[hsa:50506] [ssc:397060]
Reference
5  [PMID:10401672]
  Authors
Leseney AM, Deme D, Legue O, Ohayon R, Chanson P, Sales JP, Pires de Carvalho D, Dupuy C, Virion A.
  Title
Biochemical characterization of a Ca2+/NAD(P)H-dependent H2O2 generator in human thyroid tissue.
  Journal
Biochimie 81:373-80 (1999)
DOI:10.1016/S0300-9084(99)80084-4
Reference
6  [PMID:1995628]
  Authors
Dupuy C, Virion A, Ohayon R, Kaniewski J, Deme D, Pommier J.
  Title
Mechanism of hydrogen peroxide formation catalyzed by NADPH oxidase in thyroid plasma membrane.
  Journal
J Biol Chem 266:3739-43 (1991)
Other DBs
ExplorEnz - The Enzyme Database: 1.6.3.1
IUBMB Enzyme Nomenclature: 1.6.3.1
ExPASy - ENZYME nomenclature database: 1.6.3.1
BRENDA, the Enzyme Database: 1.6.3.1
CAS: 77106-92-4

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