KEGG   ENZYME: 1.6.3.1Help
Entry
EC 1.6.3.1                  Enzyme                                 

Name
NAD(P)H oxidase (H2O2-forming);
THOX2;
ThOX;
dual oxidase;
p138tox;
thyroid NADPH oxidase;
thyroid oxidase;
thyroid oxidase 2;
NADPH oxidase;
NAD(P)H:oxygen oxidoreductase;
NAD(P)H oxidase
Class
Oxidoreductases;
Acting on NADH or NADPH;
With oxygen as acceptor
BRITE hierarchy
Sysname
NAD(P)H:oxygen oxidoreductase (H2O2-forming)
Reaction(IUBMB)
NAD(P)H + H+ + O2 = NAD(P)+ + H2O2 [RN:R07171 R07172]
Reaction(KEGG)
Substrate
NADH [CPD:C00004];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
NAD+ [CPD:C00003];
NADP+ [CPD:C00006];
H2O2 [CPD:C00027]
Comment
Requires FAD, heme and calcium. When calcium is present, this transmembrane glycoprotein generates H2O2 by transfering electrons from intracellular NAD(P)H to extracellular molecular oxygen. The electron bridge within the enzyme contains one molecule of FAD and probably two heme groups. This flavoprotein is expressed at the apical membrane of thyrocytes, and provides H2O2 for the thyroid peroxidase-catalysed biosynthesis of thyroid hormones.
History
EC 1.6.3.1 created 2003, modified 2013
Orthology
K13411  dual oxidase
Genes
HSA: 50506(DUOX2) 53905(DUOX1)
PTR: 453395(DUOX2) 467673(DUOX1)
PPS: 100974723(DUOX1) 100978429(DUOX2)
GGO: 101137548(DUOX1) 101139037(DUOX2)
PON: 100445893(DUOX2) 100447745(DUOX1)
NLE: 100588618(DUOX1) 100595406(DUOX2)
MCC: 713126(DUOX1) 713906(DUOX2)
MCF: 102131806(DUOX2) 102133834(DUOX1)
CSAB: 103245704(DUOX2) 103245708(DUOX1)
RRO: 104653891(DUOX2) 104653893(DUOX1)
RBB: 108530131(DUOX1) 108530134(DUOX2)
CJC: 100387872(DUOX2) 100406741(DUOX1)
SBQ: 101043225(DUOX1) 101045049(DUOX2)
MMU: 214593(Duox2) 99439(Duox1)
RNO: 266807(Duox1) 79107(Duox2)
CGE: 100764526(Duox2) 100774391(Duox1)
NGI: 103746898(Duox2) 103746936(Duox1)
HGL: 101698133(Duox1) 101698889(Duox2)
CCAN: 109692712(Duox2) 109692713(Duox1)
OCU: 100344845(DUOX1) 100345610(DUOX2)
TUP: 102471164(DUOX2) 102496442(DUOX1)
CFA: 403719(DUOX2) 403720(DUOX1)
AML: 100469477(DUOX2)
UMR: 103656587(DUOX2) 103656588(DUOX1)
ORO: 101365122(DUOX2) 101365433(DUOX1)
FCA: 101087408(DUOX2) 101091374(DUOX1)
PTG: 102968075(DUOX2) 102968369(DUOX1)
BTA: 510409(DUOX1) 785698(DUOX2)
BOM: 102272102(DUOX2) 102277170(DUOX1)
BIU: 109564555(DUOX1) 109565168(DUOX2)
PHD: 102333965(DUOX1) 102334675(DUOX2)
CHX: 102179569(DUOX1) 106502554(DUOX2)
OAS: 100462743(DUOX2) 101111972(DUOX1) 101113215
SSC: 397060(DUOX2) 397177(DUOX1)
CFR: 102510558(DUOX1) 102510795(DUOX2)
CDK: 105101748(DUOX1) 105101749(DUOX2)
BACU: 103001055(DUOX2) 103001339(DUOX1)
LVE: 103071982(DUOX2) 103076766(DUOX1)
ECB: 100070541(DUOX1) 100070566(DUOX2)
EPZ: 103546007 103554035(DUOX1) 103554037(DUOX2)
EAI: 106834993(DUOX1) 106834997(DUOX2)
MYB: 102242003(DUOX2) 102252627(DUOX1) 106728032
MYD: 102771512(DUOX1) 102771797(DUOX2)
RSS: 109444262(DUOX2) 109460862(DUOX1)
PALE: 102894515(DUOX1) 102895435(DUOX2)
LAV: 100661484(DUOX2) 100677682(DUOX1)
MDO: 100012658(DUOX2) 100012836(DUOX1)
SHR: 100931267(DUOX2) 100932050(DUOX1)
OAA: 100089154(DUOX2)
GGA: 427482(DUOX2)
MGP: 100538948(DUOX2)
CJO: 107318475(DUOX2)
APLA: 101800626(DUOX2)
ACYG: 106047419(DUOX2)
TGU: 100218721(DUOX2)
GFR: 102037063(DUOX2)
FAB: 101805677(DUOX2)
PHI: 102105296(DUOX2)
PMAJ: 107209583
CCW: 104697496
FPG: 101914675(DUOX2)
FCH: 102054788(DUOX2)
CLV: 102097710(DUOX2)
EGZ: 104130687(DUOX2)
AAM: 106487968
ASN: 102372872(DUOX2)
AMJ: 102573689(DUOX2)
PSS: 102461565(DUOX2)
CMY: 102933380(DUOX2)
CPIC: 101942492(DUOX2)
ACS: 100562649
PVT: 110091655
PBI: 103059551(DUOX2)
GJA: 107106710
XLA: 108710292(duox2.L) 108711340 108712328(duox2.S)
XTR: 100486050(duox2) 100486194(duox1)
DRE: 565097(duox)
IPU: 108275020(duox1)
AMEX: 103026091(duox1)
TRU: 100124426(duox)
LCO: 104937849(duox1)
NCC: 104960088(duox1)
MZE: 101470770
OLA: 101160477(duox1)
XMA: 102217295(duox1)
PRET: 103466440(duox1)
NFU: 107388507
CSEM: 103380468(duox1)
LCF: 108876727(duox1)
HCQ: 109507657(duox1)
BPEC: 110169429
ELS: 105018355(duox1)
LCM: 102354060
CIN: 100170036(duox-d) 100170037(duox-c) 100170038(duox-b) 100170039(duox-a)
SPU: 100893745 588656(duox1)
APLC: 110980995
DME: Dmel_CG3131(Duox)
DSI: Dsimw501_GD22800(Dsim_GD22800)
MDE: 101898456
AGA: AgaP_AGAP009978(DUOX)
AAG: 5569378
AME: 551970(Duox)
BIM: 100742890
BTER: 100646100
AEC: 105143353
ACEP: 105623067
NVI: 100123854
DPA: 109541703
BMOR: 101742234
PMAC: 106717948
PRAP: 110992372
PXY: 105389437
API: 100160850
DNX: 107163754
CEL: CELE_F53G12.3(duox-2) CELE_F56C11.1(bli-3)
CBR: CBG05103(Cbr-bli-3)
BMY: Bm1_45640
TSP: Tsp_00940
OBI: 106882005
AQU: 100637823
EGR: 104451914
GMX: 100791701
VRA: 106754540
VAR: 108328293
ADU: 107484734
AIP: 107636666
LANG: 109338475
PPER: 18773495
PMUM: 103323741
ZJU: 107411138
CSV: 101207699
CMOS: 111451080
JRE: 108993958
VVI: 100265669
NTA: 107787778
NTO: 104110382
LSV: 111886578
DCR: 108221739
BVG: 104900197
PDA: 103702834
MGR: MGG_08299
PTE: PTT_09646
SMIN: v1.2.034563.t1(symbB.v1.2.034563.t1) v1.2.036935.t1(symbB.v1.2.036935.t1)
 » show all
Taxonomy
Reference
1  [PMID:12110737]
  Authors
Moreno JC, Bikker H, Kempers MJ, van Trotsenburg AS, Baas F, de Vijlder JJ, Vulsma T, Ris-Stalpers C.
  Title
Inactivating mutations in the gene for thyroid oxidase 2 (THOX2) and congenital hypothyroidism.
  Journal
N Engl J Med 347:95-102 (2002)
DOI:10.1056/NEJMoa012752
  Sequence
[hsa:50506]
Reference
2  [PMID:11822874]
  Authors
De Deken X, Wang D, Dumont JE, Miot F.
  Title
Characterization of ThOX proteins as components of the thyroid H(2)O(2)-generating system.
  Journal
Exp Cell Res 273:187-96 (2002)
DOI:10.1006/excr.2001.5444
  Sequence
[hsa:53905 50506]
Reference
3  [PMID:10806195]
  Authors
De Deken X, Wang D, Many MC, Costagliola S, Libert F, Vassart G, Dumont JE, Miot F.
  Title
Cloning of two human thyroid cDNAs encoding new members of the NADPH oxidase family.
  Journal
J Biol Chem 275:23227-33 (2000)
DOI:10.1074/jbc.M000916200
  Sequence
[hsa:53905 50506]
Reference
4  [PMID:10601291]
  Authors
Dupuy C, Ohayon R, Valent A, Noel-Hudson MS, Deme D, Virion A.
  Title
Purification of a novel flavoprotein involved in the thyroid NADPH oxidase. Cloning of the porcine and human cdnas.
  Journal
J Biol Chem 274:37265-9 (1999)
DOI:10.1074/jbc.274.52.37265
  Sequence
[hsa:50506] [ssc:397060]
Reference
5  [PMID:10401672]
  Authors
Leseney AM, Deme D, Legue O, Ohayon R, Chanson P, Sales JP, Pires de Carvalho D, Dupuy C, Virion A.
  Title
Biochemical characterization of a Ca2+/NAD(P)H-dependent H2O2 generator in human thyroid tissue.
  Journal
Biochimie 81:373-80 (1999)
DOI:10.1016/S0300-9084(99)80084-4
Reference
6  [PMID:1995628]
  Authors
Dupuy C, Virion A, Ohayon R, Kaniewski J, Deme D, Pommier J.
  Title
Mechanism of hydrogen peroxide formation catalyzed by NADPH oxidase in thyroid plasma membrane.
  Journal
J Biol Chem 266:3739-43 (1991)
Other DBs
ExplorEnz - The Enzyme Database: 1.6.3.1
IUBMB Enzyme Nomenclature: 1.6.3.1
ExPASy - ENZYME nomenclature database: 1.6.3.1
BRENDA, the Enzyme Database: 1.6.3.1
CAS: 77106-92-4

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