KEGG   ENZYME: 1.7.1.16Help
Entry
EC 1.7.1.16                 Enzyme                                 

Name
nitrobenzene nitroreductase;
cnbA (gene name)
Class
Oxidoreductases;
Acting on other nitrogenous compounds as donors;
With NAD+ or NADP+ as acceptor
BRITE hierarchy
Sysname
N-phenylhydroxylamine:NADP+ oxidoreductase
Reaction(IUBMB)
N-phenylhydroxylamine + 2 NADP+ + H2O = nitrobenzene + 2 NADPH + 2 H+ (overall reaction) [RN:R11744];
(1a) N-phenylhydroxylamine + NADP+ = nitrosobenzene + NADPH + H+ [RN:R05400];
(1b) nitrosobenzene + NADP+ + H2O = nitrobenzene + NADPH + H+ [RN:R05401]
Reaction(KEGG)
Substrate
N-phenylhydroxylamine [CPD:C02720];
NADP+ [CPD:C00006];
H2O [CPD:C00001];
nitrosobenzene [CPD:C06876]
Product
nitrobenzene [CPD:C06813];
NADPH [CPD:C00005];
H+ [CPD:C00080];
nitrosobenzene [CPD:C06876]
Comment
Contains FMN. The enzyme, characterized from Pseudomonas species, catalyses two successive reductions of nitrobenzene, via a nitrosobenzene intermediate. It is also active on 1-chloro-4-nitrobenzene.
History
EC 1.7.1.16 created 2017
Pathway
ec00627  Aminobenzoate degradation
ec01120  Microbial metabolism in diverse environments
Orthology
K15057  nitrobenzene nitroreductase
Reference
1  [PMID:7601851]
  Authors
Somerville CC, Nishino SF, Spain JC
  Title
Purification and characterization of nitrobenzene nitroreductase from Pseudomonas pseudoalcaligenes JS45.
  Journal
J Bacteriol 177:3837-42 (1995)
DOI:10.1128/JB.177.13.3837-3842.1995
Reference
2  [PMID:16517619]
  Authors
Wu JF, Jiang CY, Wang BJ, Ma YF, Liu ZP, Liu SJ
  Title
Novel partial reductive pathway for 4-chloronitrobenzene and nitrobenzene degradation in Comamonas sp. strain CNB-1.
  Journal
Appl Environ Microbiol 72:1759-65 (2006)
DOI:10.1128/AEM.72.3.1759-1765.2006
Other DBs
ExplorEnz - The Enzyme Database: 1.7.1.16
IUBMB Enzyme Nomenclature: 1.7.1.16
ExPASy - ENZYME nomenclature database: 1.7.1.16
BRENDA, the Enzyme Database: 1.7.1.16

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