KEGG ENZYME: 1.7.1.6

ENZYME: 1.7.1.6

Entry

EC 1.7.1.6                  Enzyme

Name

azobenzene reductase;
new coccine (NC)-reductase;
NC-reductase;
azo-dye reductase;
orange II azoreductase;
NAD(P)H:1-(4'-sulfophenylazo)-2-naphthol oxidoreductase;
orange I azoreductase;
azo reductase;
azoreductase;
nicotinamide adenine dinucleotide (phosphate) azoreductase;
NADPH2-dependent azoreductase;
dimethylaminobenzene reductase;
p-dimethylaminoazobenzene azoreductase;
dibromopropylaminophenylazobenzoic azoreductase;
N,N-dimethyl-4-phenylazoaniline azoreductase;
p-aminoazobenzene reductase;
methyl red azoreductase;
NADPH2:4-(dimethylamino)azobenzene oxidoreductase

Class

Oxidoreductases;
Acting on other nitrogenous compounds as donors;
With NAD+ or NADP+ as acceptor

Sysname

N,N-dimethyl-1,4-phenylenediamine, aniline:NADP+ oxidoreductase

Reaction(IUBMB)

N,N-dimethyl-1,4-phenylenediamine + aniline + 2 NADP+ = 4-(dimethylamino)azobenzene + 2 NADPH + 2 H+ [RN:R05711]

Reaction(KEGG)

R05711

Substrate

N,N-dimethyl-1,4-phenylenediamine [CPD:C04203];
aniline [CPD:C00292];
NADP+ [CPD:C00006]

Product

4-(dimethylamino)azobenzene;
NADPH [CPD:C00005];
H+ [CPD:C00080]

Comment

The reaction occurs in the reverse direction to that shown above. Other azo dyes, such as Methyl Red, Rocceline, Solar Orange and Sumifix Black B can also be reduced [2].

History

EC 1.7.1.6 created 1961 as EC 1.6.6.7, transferred 2002 to EC 1.7.1.6

Orthology

K03206

azobenzene reductase

Genes

BSU:	BSU09340(yhdA)

BSR:

I33_1061

BSL:

A7A1_3793

BSH:	BSU6051_09340(yhdA)

BSY:	I653_04695

BSUT:

BSUB_01029(yhdA)

BSUL:

BSUA_01029(yhdA)

BSUS:

Q433_05305

BSO:	BSNT_07366(yhdA)

BSN:	BSn5_16550

BSQ:	B657_09340(yhdA)

BSP:	U712_04755

BSS:	BSUW23_04715(yhdA)

BST:

GYO_1222

BIT:	BIS30_15360

BLI:	BL02896(azr)

BLD:	BLi01002(yhdA)

BLH:	BaLi_c11070(yhdA)

BAY:	RBAM_009600

BAQ:	BACAU_0920(yhdA)

BYA:	BANAU_0865(yhdA)

BAMP:

B938_04585

BQY:	MUS_0972(azr)

BAML:

BAM5036_0862(yhdA)

BAMA:

RBAU_0923(yhdA)

BAMN:

BASU_0899(yhdA)

BAMB:

BAPNAU_2842(yhdA)

BAMT:

AJ82_05370

BAMY:

V529_08950(yhdA)

BMP:	NG74_00967(azr_1)

BAO:	BAMF_1030(yhdA)

BAZ:	BAMTA208_04360(yhdA)

BQL:	LL3_01015(yhdA)

BXH:	BAXH7_00910(azr)

BAMI:

KSO_015075

BAMC:

U471_09420

BAMF:

U722_04840

BSIA:

CWD84_16970

BAE:	BATR1942_02270

BVM:	B9C48_04785

BSON:

S101395_03831

BHT:	DIC78_04980

BMOJ:

HC660_09930(azr)

BIQ:	AN935_04885

BSTR:

QI003_05115(yhdA)

BAN:

BA_2276

BAR:

GBAA_2276

BAT:

BAS2122

BAH:	BAMEG_2317

BAI:

BAA_2341

BAX:	H9401_2164

BANT:

A16_23150

BANR:

A16R_23410

BANS:

BAPAT_2177

BANH:

HYU01_11355

BANV:

DJ46_1075(azr)

BCE:

BC2229

BCA:

BCE_2310

BCZ:	BCE33L2056(azr)

BCR:	BCAH187_A2386

BCB:	BCB4264_A2259

BCU:	BCAH820_2302

BCG:	BCG9842_B3066

BCQ:	BCQ_2213(azr)

BCX:

BCA_2358

BAL:	BACI_c22390(azr)

BNC:

BCN_2204

BCF:

bcf_11405

BCER:

BCK_23455

BCY:	Bcer98_1668

BTK:	BT9727_2060(azr)

BTL:

BALH_2037

BTB:	BMB171_C2006

BTT:

HD73_2517

BTHR:

YBT1520_12310

BTHI:

BTK_12480

BTC:	CT43_CH2195

BTF:	YBT020_11650

BTM:	MC28_1448(azr)

BTG:	BTB_c23090(azr)

BTI:

BTG_08730

BTN:	BTF1_08650

BTHT:

H175_ch2229

BTHU:

YBT1518_12380

BTW:	BF38_3479(azr)

BTHY:

AQ980_18475

BWE:	BcerKBAB4_2096

BWW:	bwei_2769(azr)

BMYO:

BG05_3765(azr)

BTY:	Btoyo_4861

BMYC:

DJ92_4824(azr)

BBY:	CY96_10270

BWD:	CT694_12080

BTRO:

FJR70_08255

BMOB:

MLA2C4_11380

BNT:	GSN03_10925

BPAC:

LMD38_15365

BPAN:

NLJ82_10990

BALU:

QRY64_13665

BIF:	N288_06240

BGY:	BGLY_1031(azr2)

BWH:	A9C19_16125

BSHI:

LGQ02_10715

BCAB:

EFK13_05315(yhdA)

BRY:	M0696_05280(yhdA)

BJS:

MY9_1030

BACP:

SB24_05080

BACB:

OY17_07480

BACY:

QF06_03610

BACL:

BS34A_10540(yhdA)

BALM:

BsLM_0974

BACS:

AUL54_05810

BHAI:

KJK41_06785

BCAO:

LC087_00425

BARD:

QRY57_12640

BHK:	B4U37_05830

BCOH:

BC6307_03250

BMQ:

BMQ_2501

BMD:

BMD_2488

BMH:	BMWSH_2707(yhdA)

BMEG:

BG04_4948(azr)

BEO:

BEH_03225

PKS:	IE339_03025

BGI:	BGM20_19950

BON:	A361_06560

CSUA:

IM538_03800

CFIR:

NAF01_06220

CSPG:

LS684_04705

CPSS:

M5V91_15200

GWC:	GWCH70_0533

PTB:	DER53_07345

PCAL:

BV455_01196(azr)

ANM:	GFC28_1971(azr)

AAMY:

GFC30_959(azr)

ANL:	GFC29_3463(azr)

AND:	GRQ40_03375

ACAI:

ISX45_00235

HMN:	HM131_17095

HLI:

HLI_20990

FPN:	ABE65_002665

FAR:	ABE41_002580

FEC:	QNH15_05820

BSJ:	UP17_04685

BMUR:

ABE28_004860

PBUT:

DTO10_17155

PASA:

BAOM_0840(azr)

PFRI:

L8956_05065

PERI:

RE409_05270

POF:	GS400_03955

PCHU:

QNI29_03955

PONA:

IMZ31_03345

MEKU:

HUW50_12430

MDG:	K8L98_04125

MLIT:

KDJ21_001395

MENL:

MVE64_10175 MVE64_16920

MEBZ:

LPC09_03420

BVQ:	FHE72_07200

RMF:	D5E69_07875

RAZ:	U9J35_07215

ROD:	P8596_07420

ROSS:

V2W31_07145

MCUI:

G8O30_02185

GAJ:	MY490_11890 MY490_20380

ALKG:

MOJ78_16785

PHJ:	LC071_15595

BCOP:

JD108_00045

BHUI:

LOK74_16820

ANX:	ACH33_08170

ATHE:

K3F53_10665

PAEK:

D3873_10075

PANC:

E2636_04315

LFB:	C1X05_06860

ACIT:

HPK19_06305 HPK19_16115

» show all

Reference

1 [PMID:18139207]

Authors

MUELLER GC, MILLER JA

Title

The reductive cleavage of 4-dimethylaminoazobenzene by rat liver; the intracellular distribution of the enzyme system and its requirement for triphosphopyridine nucleotide.

Journal

J Biol Chem 180:1125-36 (1949)

Reference

2 [PMID:11134015]

Authors

Suzuki Y, Yoda T, Ruhul A, Sugiura W

Title

Molecular cloning and characterization of the gene coding for azoreductase from Bacillus sp. OY1-2 isolated from soil.

Journal

J Biol Chem 276:9059-65 (2001)
DOI:10.1074/jbc.M008083200

Sequence

[ag:BAB13746]