KEGG ENZYME: 1.7.3.1

ENZYME: 1.7.3.1

Entry

EC 1.7.3.1                  Enzyme

Name

nitroalkane oxidase;
nitroethane oxidase;
NAO;
nitroethane:oxygen oxidoreductase

Class

Oxidoreductases;
Acting on other nitrogenous compounds as donors;
With oxygen as acceptor

Sysname

nitroalkane:oxygen oxidoreductase

Reaction(IUBMB)

a nitroalkane + H2O + O2 = an aldehyde or ketone + nitrite + H2O2 [RN:R10388 R10389]

Reaction(KEGG)

R10388 > R00799;
R10389

Substrate

nitroalkane [CPD:C06058];
H2O [CPD:C00001];
O2 [CPD:C00007]

Product

aldehyde [CPD:C00071];
ketone [CPD:C01450];
nitrite [CPD:C00088];
H2O2 [CPD:C00027]

Comment

Has an absolute requirement for FAD [4]. While nitroethane may be the physiological substrate [2], the enzyme also acts on several other nitroalkanes, including 1-nitropropane, 2-nitropropane, 1-nitrobutane, 1-nitropentane, 1-nitrohexane, nitrocyclohexane and some nitroalkanols [4]. Differs from EC 1.13.12.16, nitronate monooxygenase, in that the preferred substrates are neutral nitroalkanes rather than anionic nitronates [4].

History

EC 1.7.3.1 created 1961, modified 2006, modified 2009

Pathway

ec00910

Nitrogen metabolism

ec01100

Metabolic pathways

Orthology

K19823

nitroalkane oxidase

Genes

PAN:	PODANSg2158 PODANSg4289

TMN:	UCRPA7_3412

FGR:	FGSG_02379

FPU:	FPSE_06845

FVR:	FVEG_05969

FOX:	FOXG_08703

NHE:	NECHADRAFT_81017

FFC:	NCS54_00761100

CFJ:	CFIO01_03827

CHIG:

CH63R_08266

ELA:	UCREL1_3529

PFY:	PFICI_01984

SSL:	SS1G_09730

BFU:	BCIN_07g06070

PSCO:

LY89DRAFT_617478 LY89DRAFT_709519

GLZ:	GLAREA_03183

ANI:

AN9162.2

NFI:	NFIA_030710

AFV:	AFLA_013415

TMF:	EYB26_001336

TRG:	TRUGW13939_02491

PNO:	SNOG_15929(SNOG_15928)

BZE:	COCCADRAFT_81440

BSC:	COCSADRAFT_112376

BOR:	COCMIDRAFT_92255

FFU:	CLAFUR5_09879

BCOM:

BAUCODRAFT_39353

NPA:	UCRNP2_4550

ADL:	AURDEDRAFT_71413

PLAL:

FXN65_13835

VBO:	CKY39_23140

MPT:

Mpe_A2676

METP:

C1M51_14750

HSE:	Hsero_0937

HSZ:	ACP92_04690

HRB:	Hrubri_0798

AVV:	RvVAT039_pl02080

AVI:

Avi_7637

BBT:

BBta_3103

BRS:

S23_32530

BRK:	CWS35_13395

BSYM:

CIT39_16315

BSEP:

HAP48_0007595

BQB:	J4P68_0028370

BBAN:

J4G43_048315

BCOU:

IC761_06750 IC761_06755 IC761_34095

PSIN:

CAK95_27115

NPN:	JI59_19965

RHA:	RHA1_ro05247

RHB:

NY08_1957

RKO:	JWS14_36595

PDEF:

P9209_06210

SPIN:

KV203_00865

SQZ:	FQU76_30855

STSU:

B7R87_30865

LSE:	F1C12_09540

ARL:	AFL94_04845

GPR:	JQN66_05285

GNC:	QQS42_08525

PSIM:

KR76_25720

PDX:	Psed_2734 Psed_2948

PSEA:

WY02_17450

PBRO:

HOP40_05335 HOP40_07760

PPEL:

H6H00_00610 H6H00_09505

BALA:

DSM104299_04129(acdA_3)

» show all

Reference

1 [PMID:14907722]

Authors

LITTLE HN.

Title

Oxidation of nitroethane by extracts from Neurospora.

Journal

J Biol Chem 193:347-58 (1951)

Reference

2 [PMID:22538]

Authors

Kido T, Hashizume K, Soda K.

Title

Purification and properties of nitroalkane oxidase from Fusarium oxysporum.

Journal

J Bacteriol 133:53-8 (1978)
DOI:10.1128/JB.133.1.53-58.1978

Reference

3 [PMID:11867731]

Authors

Daubner SC, Gadda G, Valley MP, Fitzpatrick PF

Title

Cloning of nitroalkane oxidase from Fusarium oxysporum identifies a new member of the acyl-CoA dehydrogenase superfamily.

Journal

Proc Natl Acad Sci U S A 99:2702-7 (2002)
DOI:10.1073/pnas.052527799

Sequence

[ag:AAL57485]

Reference

4 [PMID:15581574]

Authors

Fitzpatrick PF, Orville AM, Nagpal A, Valley MP.

Title

Nitroalkane oxidase, a carbanion-forming flavoprotein homologous to acyl-CoA dehydrogenase.

Journal

Arch Biochem Biophys 433:157-65 (2005)
DOI:10.1016/j.abb.2004.08.021

Reference

5 [PMID:15713081]

Authors

Valley MP, Tichy SE, Fitzpatrick PF.

Title

Establishing the kinetic competency of the cationic imine intermediate in nitroalkane oxidase.

Journal

J Am Chem Soc 127:2062-6 (2005)
DOI:10.1021/ja043542f

Other DBs

ExplorEnz - The Enzyme Database:

1.7.3.1

IUBMB Enzyme Nomenclature:

1.7.3.1

ExPASy - ENZYME nomenclature database:

1.7.3.1

UM-BBD (Biocatalysis/Biodegradation Database):

1.7.3.1

BRENDA, the Enzyme Database:

1.7.3.1

CAS:	9029-36-1 65802-82-6

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