KEGG ENZYME: 1.8.4.9

ENZYME: 1.8.4.9

Entry

EC 1.8.4.9                  Enzyme

Name

adenylyl-sulfate reductase (glutathione);
5'-adenylylsulfate reductase (also used for EC 1.8.99.2);
AMP,sulfite:oxidized-glutathione oxidoreductase (adenosine-5'-phosphosulfate-forming);
plant-type 5'-adenylylsulfate reductase

Class

Oxidoreductases;
Acting on a sulfur group of donors;
With a disulfide as acceptor

Sysname

AMP,sulfite:glutathione-disulfide oxidoreductase (adenosine-5'-phosphosulfate-forming)

Reaction(IUBMB)

AMP + sulfite + glutathione disulfide = adenylyl sulfate + 2 glutathione [RN:R05717]

Reaction(KEGG)

R05717

Substrate

AMP [CPD:C00020];
sulfite [CPD:C00094];
glutathione disulfide [CPD:C00127]

Product

adenylyl sulfate [CPD:C00224];
glutathione [CPD:C00051]

Comment

This enzyme differs from EC 1.8.99.2, adenylyl-sulfate reductase, in using glutathione as the reductant. Glutathione can be replaced by gamma-glutamylcysteine or dithiothreitol, but not by thioredoxin, glutaredoxin or 2-sulfanylethan-1-ol (2-mercaptoethanol). The enzyme from the mouseear cress, Arabidopsis thaliana, contains a glutaredoxin-like domain. The enzyme is also found in other photosynthetic eukaryotes, e.g., the Madagascar periwinkle, Catharanthus roseus and the hollow green seaweed, Ulva intestinalis.

History

EC 1.8.4.9 created 2000, modified 2002

Pathway

ec00920

Sulfur metabolism

ec01100

Metabolic pathways

ec01120

Microbial metabolism in diverse environments

Orthology

K05907

adenylyl-sulfate reductase (glutathione)

Genes

ATH:	AT1G62180(APR2) AT4G04610(APR1) AT4G21990(APR3)

ALY:	9303872 9310871 9322553

CRB:	17880982 17883322 17896630

CSAT:

104703816 104707608 104714551 104716805 104718119 104722840 104731320 104737308 104752870

EUS:	EUTSA_v10023459mg EUTSA_v10025134mg EUTSA_v10028642mg

BRP:	103839826 103848153 103859677 103861274

BNA:	106347879 106366895 106396567 106418329 106420885 106435512 106440433 106442702

BOE:	106306507 106310441 106319498 106342464

RSZ:	108824435 108829478 108842851 108848746 108851680

THJ:	104806307 104809496 104818752

CPAP:

110808072 110820839 110820876

CIT:	102626298 102626350

CIC:	CICLE_v10010222mg CICLE_v10015162mg

PVY:	116137243 116142891 116142895

MINC:

123192770 123219166

TCC:

18597168

GRA:	105769511 105773448

GHI:	107900590 107905918 107958280 107961997

GAB:	108452964 108486674

HSYR:

120131916 120175371 120190639 120208754

DZI:	111275205 111278275

EGR:

104441413

RARG:

115742949

GMX:	100799978 100819536 547630

GSJ:	114367269 114387349 114420178

PVU:	137806711 137832221

VRA:	106766568 106775990

VAR:	108336023 108343304

VUN:	114174957 114187852

VUM:	124833619 124834153

CCAJ:

109803660 109809738

APRC:

113846354 113847166

MTR:

25486175

TPRA:

123894324 123908525

CAM:

101505158

PSAT:

127077889 127138358

VVO:	131595436 131599909 131636758

LJA:	130711788 130723596

ADU:	107466613 107479096 107482990

AIP:	107636252 107638633

AHF:	112705822 112722891 112741226 112795922

LANG:

109329867 109333726 109342504 109345338

PCIN:

129285121 129292286

QSA:	O6P43_000567 O6P43_011930 O6P43_031253

FVE:

101296464

AANS:

126788197

RCN:

112165291

PPER:

18778340 18779258

PMUM:

103324412 103324413 103326433

PAVI:

110748229 110762244 110763068

PDUL:

117624010 117624459

MDM:	103408766 103409471

MSYL:

126586416 126615868 126623428

PXB:	103948191 103949894 103951376

ZJU:	107407239 107420715

MNT:	112095244 21391473 21401025 21401026

CSAV:

115707749

CSV:	101211864 101220567

CMO:	103484617 103485224

BHJ:	120072791 120083844

MCHA:

111010712 111012338

CMAX:

111477996 111490004 111495445

CMOS:

111431516 111453522 111456956

CPEP:

111783406 111802499 111803510

RCU:

8284186

MEAN:

126664476

HBR:	110639463 110670643

MESC:

110602822 110605077

POP:	18097384 7462058

PEU:	105134776 105141663

PALZ:

118040296 118058881

PNZ:	133682482 133699190

JRE:	108985688 109006940 109020834

CILL:

122276700 122278091

CAVE:

132170107 132172106 132176235

AGLU:

133868619 133874904

QSU:	112014192 112030971

QLO:	115957421 115989634

TWL:	119989640 119990033

VVI:	100233045 100854884

VRI:	117923987 117926670

SLY:	101252466 101257319 544267

SPEN:

107011261 107011717 107012556

SOT:	102579112 102587128 102600029(apsr1)

SSTN:

125847615 125848423 125860505

SDUL:

129872912 129873523

CANN:

107858434 107859594

LBB:	132606308 132621429

NTA:	107768720 107789753 107799658 107801230 107811452 107829885

NSY:	104218889 104248308 104249938

NTO:	104098181 104106256 104111505

NAU:	109212240 109222466 109238139 109245027

INI:	109164888 109165701 109167270

ITR:	116027425 116029479 116032225

SIND:

105167991 105175419

OEU:	111395949 111395977 111402425

EGT:	105962230 105968964

SSPL:

121745534 121775254 121783581 121787533 121808601

SMIL:

130985803 131011202

SHIS:

125187933 125195967 125213990

APAN:

127253284 127254262

HAN:	110910798 110918325 110939621

ECAD:

122590947 122595134

LSV:	111891058 111907858

CCAV:

112522741 112525036

DCR:	108204316 108210263

CSIN:

114259072 114263981 114276131

RVL:	131315278 131330502

AEW:	130783955 130794434

BVG:

104900978

SOE:

110792539

CQI:	110687384 110703043 110720709 110731321

ATRI:

130815262 130824094

MOF:	131152778 131154487

NNU:	104588316 104612772

MING:

122059929 122092831

TSS:	122639623 122643127

PSOM:

113287967 113305261 113315016 113348553

OSA:	4343348(APR-like1)

DOSA:

Os07g0509800

OBR:

102709478

OGL:

127780141

BDI:

100828269

ATS:

109738044

TDC:	119354341 119363511

TAES:

123044707 123052570 123188457

TUA:

125537252

LPER:

127333356

LRD:

124670460

SBI:

8080734

ZMA:	103647705 606419

SITA:

101776145

SVS:

117843700

PVIR:

120661463 120689191

PHAI:

112881988

PAUA:

133890698 133892505

PDA:	103707194 103719146

EGU:	105036141 105050935

MUS:

135605861

ZOF:	121972637 121978143 122020514 122024581 122040663 122045204 122050702

DCT:

110111790

PEQ:

110030804

AOF:	109833481 109849392

MSIN:

131240625

ACAK:

137936919 137941475

NCOL:

116246043

ATR:

18445736

CJF:

131029694

SMO:	SELMODRAFT_167572 SELMODRAFT_187782

PPP:

112281535

CRE:	CHLRE_12g517150v5

VCN:	VOLCADRAFT_103701

CSL:	COCSUDRAFT_22587

CVR:	CHLNCDRAFT_33215

APRO:

F751_6755

OLU:	OSTLU_13445(APR1)

OTA:	OT_ostta14g00360

BPG:	Bathy04g04910

MIS:	MICPUN_87762

MPP:	MICPUCDRAFT_53472

CME:	CYME_CMT162C

GSL:	Gasu_09200

CCP:	CHC_T00009224001

» show all

Reference

1 [PMID:8917599]

Authors

Gutierrez-Marcos JF, Roberts MA, Campbell EI, Wray JL

Title

Three members of a novel small gene-family from Arabidopsis thaliana able to complement functionally an Escherichia coli mutant defective in PAPS reductase activity encode proteins with a thioredoxin-like domain and "APS reductase" activity.

Journal

Proc Natl Acad Sci U S A 93:13377-82 (1996)
DOI:10.1073/pnas.93.23.13377

Sequence

[ath:AT4G04610 AT1G62180 AT4G21990]

Reference

2 [PMID:8917600]

Authors

Setya A, Murillo M, Leustek T

Title

Sulfate reduction in higher plants: molecular evidence for a novel 5'-adenylylsulfate reductase.

Journal

Proc Natl Acad Sci U S A 93:13383-8 (1996)
DOI:10.1073/pnas.93.23.13383