KEGG   ENZYME: 1.8.5.5Help
Entry
EC 1.8.5.5                  Enzyme                                 

Name
thiosulfate reductase (quinone);
phsABC (gene names)
Class
Oxidoreductases;
Acting on a sulfur group of donors;
With a quinone or similar compound as acceptor
BRITE hierarchy
Sysname
sulfite,hydrogen sulfide:quinone oxidoreductase
Reaction(IUBMB)
sulfite + hydrogen sulfide + a quinone = thiosulfate + a quinol [RN:R11488]
Reaction(KEGG)
Substrate
sulfite [CPD:C00094];
hydrogen sulfide [CPD:C00283];
quinone [CPD:C15602]
Product
thiosulfate [CPD:C00320];
quinol [CPD:C15603]
Comment
The enzyme, characterized from the bacterium Salmonella enterica, is similar to EC 1.17.5.3, formate dehydrogenase-N. It contains a molybdopterin-guanine dinucleotide, five [4Fe-4S] clusters and two heme b groups. The reaction occurs in vivo in the direction of thiosulfate disproportionation, which is highly endergonic. It is driven by the proton motive force that occurs across the cytoplasmic membrane.
History
EC 1.8.5.5 created 2016, modified 2017
Pathway
ec00920  Sulfur metabolism
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K08352  thiosulfate reductase / polysulfide reductase chain A
Genes
ECY: ECSE_P3-0024
STY: STY2271(phsA)
STT: t0809(phsA)
SEX: STBHUCCB_8630
SENT: TY21A_04105
STM: STM2065(phsA)
SEO: STM14_2555(phsA)
SEV: STMMW_20951
SEY: SL1344_2041(phsA)
SEM: STMDT12_C20870
SEJ: STMUK_2095(phsA)
SEB: STM474_2150(phsA)
SEF: UMN798_2231(phsA)
SENR: STMDT2_20381(phsA)
SEND: DT104_21221(phsA)
SENI: CY43_11140
SEI: SPC_1649(phsA)
SEC: SCH_2074(phsA)
SHB: SU5_02659
SENS: Q786_10195
SED: SeD_A2401(phsA)
SEG: SG2094(phsA)
SEL: SPUL_0835(phsA)
SEGA: SPUCDC_0835(phsA)
SET: SEN2063(phsA)
SENA: AU38_10410
SENO: AU37_10420
SENV: AU39_10420
SENQ: AU40_11665
SENL: IY59_10705
SENB: BN855_21500(phsA)
SENE: IA1_10305
SBG: SBG_1889(phsA)
SBZ: A464_2189
SALZ: EOS98_08685(phsA)
CPOT: FOB25_20400(phsA)
PMR: PMI1814(phsA)
PMIB: BB2000_1921(phsA)
PHAU: PH4a_01215
PCIB: F9282_12780(phsA)
ETR: ETAE_1845(phsA)
ETD: ETAF_1665
ETE: ETEE_3891
LRI: NCTC12151_02197(psrA)
SON: SO_4062(phsA)
SFR: Sfri_1999
SAZ: Sama_3177
SLO: Shew_0608
SSE: Ssed_4047
SWP: swp_1238
SLJ: EGC82_11685(phsA)
SMAI: EXU30_13320(phsA)
FBL: Fbal_3636
AEH: Mlg_0633
HHA: Hhal_1166
WSU: WS0116(fdhA) WS0709(fdhA)
SULC: CVO_09640
CGRA: CGRAC_0560
CURE: CUREO_1061
CHYO: CHH_0519
CSPF: CSF_0471
NAM: NAMH_1518
GLO: Glov_1164
DEU: DBW_0837
DVU: DVU0173
DVL: Dvul_2794
DDS: Ddes_1667
DMA: DMR_08220(phaA)
DGG: DGI_0241
DPI: BN4_20079
DRT: Dret_0895
DML: Dmul_02650(phsA)
DTO: TOL2_C03980(phsA)
ADE: Adeh_2632
DBR: Deba_2842
ACR: Acry_0345
AMV: ACMV_03760(psrA)
BBEV: BBEV_1018(psrA-1)
BSE: Bsel_1476
STH: STH109
DSY: DSY3896
DHD: Dhaf_1509
CHY: CHY_2574
MTA: Moth_0450
TACI: TDSAC_1714
LPIL: LIP_1347
GPA: GPA_25490
TTH: TT_C0155
OTE: Oter_1466
MBAS: ALGA_3219
CCH: Cag_0620
CLI: Clim_2400
PPH: Ppha_0912
PAA: Paes_2139
PROC: Ptc2401_02170(psrA)
IAL: IALB_1663
MRO: MROS_1774
CABY: Cabys_1269
CTHI: THC_0921
HDF: AArcSl_0127(phsA) AArcSl_1905(phsA2)
 » show all
Taxonomy
Reference
1  [PMID:6384182]
  Authors
Kwan HS, Barrett EL
  Title
Map locations and functions of Salmonella typhimurium men genes.
  Journal
J Bacteriol 159:1090-2 (1984)
Reference
2  [PMID:3108233]
  Authors
Clark MA, Barrett EL
  Title
The phs gene and hydrogen sulfide production by Salmonella typhimurium.
  Journal
J Bacteriol 169:2391-7 (1987)
DOI:10.1128/JB.169.6.2391-2397.1987
Reference
3  [PMID:7737516]
  Authors
Alami N, Hallenbeck PC
  Title
Cloning and characterization of a gene cluster, phsBCDEF, necessary for the production of hydrogen sulfide from thiosulfate by Salmonella typhimurium.
  Journal
Gene 156:53-7 (1995)
DOI:10.1016/0378-1119(94)00930-Q
  Sequence
Reference
4  [PMID:7751291]
  Authors
Heinzinger NK, Fujimoto SY, Clark MA, Moreno MS, Barrett EL
  Title
Sequence analysis of the phs operon in Salmonella typhimurium and the contribution of thiosulfate reduction to anaerobic energy metabolism.
  Journal
J Bacteriol 177:2813-20 (1995)
DOI:10.1128/JB.177.10.2813-2820.1995
  Sequence
Reference
5  [PMID:22081391]
  Authors
Stoffels L, Krehenbrink M, Berks BC, Unden G
  Title
Thiosulfate reduction in Salmonella enterica is driven by the proton motive force.
  Journal
J Bacteriol 194:475-85 (2012)
DOI:10.1128/JB.06014-11
Other DBs
ExplorEnz - The Enzyme Database: 1.8.5.5
IUBMB Enzyme Nomenclature: 1.8.5.5
ExPASy - ENZYME nomenclature database: 1.8.5.5
BRENDA, the Enzyme Database: 1.8.5.5

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