KEGG ENZYME: 1.8.5.5

ENZYME: 1.8.5.5

Entry

EC 1.8.5.5                  Enzyme

Name

thiosulfate reductase (quinone);
phsABC (gene names)

Class

Oxidoreductases;
Acting on a sulfur group of donors;
With a quinone or similar compound as acceptor

Sysname

sulfite,hydrogen sulfide:quinone oxidoreductase

Reaction(IUBMB)

sulfite + hydrogen sulfide + a quinone = thiosulfate + a quinol [RN:R11488]

Reaction(KEGG)

R11488 > R10149

Substrate

sulfite [CPD:C00094];
hydrogen sulfide [CPD:C00283];
quinone [CPD:C15602]

Product

thiosulfate [CPD:C00320];
quinol [CPD:C15603]

Comment

The enzyme, characterized from the bacterium Salmonella enterica, is similar to EC 1.17.5.3, formate dehydrogenase-N. It contains a molybdopterin-guanine dinucleotide, five [4Fe-4S] clusters and two heme b groups. The reaction occurs in vivo in the direction of thiosulfate disproportionation, which is highly endergonic. It is driven by the proton motive force that occurs across the cytoplasmic membrane.

History

EC 1.8.5.5 created 2016, modified 2017

Pathway

ec00920

Sulfur metabolism

ec01100

Metabolic pathways

ec01120

Microbial metabolism in diverse environments

Orthology

K08352

thiosulfate reductase / polysulfide reductase chain A

Genes

ECY:	ECSE_P3-0024

STY:	STY2271(phsA)

STT:	t0809(phsA)

SEX:	STBHUCCB_8630

SENT:

TY21A_04105

STM:	STM2065(phsA)

SEO:	STM14_2555(phsA)

SEV:	STMMW_20951

SEY:	SL1344_2041(phsA)

SEM:	STMDT12_C20870

SEJ:	STMUK_2095(phsA)

SEB:	STM474_2150(phsA)

SEF:	UMN798_2231(phsA)

SETU:

STU288_06715

SETC:

CFSAN001921_06460

SENR:

STMDT2_20381(phsA)

SEND:

DT104_21221(phsA)

SENI:

CY43_11140

SEEN:

SE451236_16535

SPQ:	SPAB_01038

SEI:	SPC_1649(phsA)

SEC:	SCH_2074(phsA)

SEH:	SeHA_C2288

SHB:

SU5_02659

SENH:

CFSAN002069_21495

SEEH:

SEEH1578_19605

SEE:	SNSL254_A2242

SENN:

SN31241_31660

SEW:	SeSA_A2235

SEA:	SeAg_B2188

SENS:

Q786_10195

SED:	SeD_A2401(phsA)

SEG:	SG2094(phsA)

SEL:	SPUL_0835(phsA)

SEGA:

SPUCDC_0835(phsA)

SET:	SEN2063(phsA)

SENA:

AU38_10410

SENO:

AU37_10420

SENV:

AU39_10420

SENQ:

AU40_11665

SENL:

IY59_10705

SENJ:

CFSAN001992_01110

SEEC:

CFSAN002050_17285

SEEB:

SEEB0189_009140

SENB:

BN855_21500(phsA)

SENE:

IA1_10305

SENC:

SEET0819_17470

SES:	SARI_00822

SBG:	SBG_1889(phsA)

SBZ:

A464_2189

SBV:	N643_09165

SALZ:

EOS98_08685(phsA)

CFD:	CFNIH1_21320

CBRA:

A6J81_00990

CWE:	CO701_07905

CYO:	CD187_09090

CPOT:

FOB25_20400(phsA)

CIF:	AL515_09925

CIE:	AN232_13865

CPAR:

CUC49_05300

CITZ:

E4Z61_02730(phsA)

CARS:

E1B03_16850(phsA)

CIX:	M4I31_09015(phsA)

CIB:	HF677_009265(phsA)

CITR:

GUC46_08955(phsA)

PMR:	PMI1814(phsA)

PMIB:

BB2000_1921(phsA)

PVL:	AOB99_11090

PVG:	CRN77_09800

PHAU:

PH4a_01215

PROT:

BTA34_10765

PCOL:

F1325_12680(phsA)

PCIB:

F9282_12780(phsA)

PPEE:

I6G31_15405(phsA)

PAPP:

QQS39_12760(phsA)

EIC:	NT01EI_1854

ETD:

ETAF_1665

ETE:

ETEE_3891

ETR:	ETAE_1845(phsA)

ETC:	ETAC_08455

EDW:	QY76_11045

EDL:	AAZ33_09395(phsA)

EHO:	A9798_08680

LRI:	NCTC12151_02197(psrA)

LGB:	V8L73_11840(phsA)

PGB:	H744_2c2539

PLUL:

FOB45_15630

MARA:

D0851_14340

SON:	SO_4062(phsA)

SFR:

Sfri_1999

SAZ:

Sama_3177

SBM:	Shew185_0531

SBN:	Sbal195_0555

SBP:	Sbal223_0559

SBT:	Sbal678_0563

SBB:	Sbal175_0622

SLO:

Shew_0608

SHP:	Sput200_0542

SSE:

Ssed_4047

SPL:	Spea_3039 Spea_3188

SHE:	Shewmr4_3444

SHM:	Shewmr7_0507

SHN:	Shewana3_3619

SHL:	Shal_3127 Shal_3271

SWP:

swp_1238

SHF:	CEQ32_19375

SJA:	SJ2017_3760

SBJ:	CF168_02695

SMAV:

CFF01_02980

SHEW:

CKQ84_11085

SALG:

BS332_04285

SLJ:	EGC82_11685(phsA)

SMAI:

EXU30_13320(phsA)

SPOL:

FH971_10120(phsA)

SKH:	STH12_04225(psrA)

SLIT:

JQC75_16275(phsA)

SCAA:

TUM17387_33610(phsA)

SPSH:

FM037_24400(phsA)

SEUR:

FM038_002915(phsA)

SYK:	KDN34_15170(phsA)

SAVI:

JYB87_15740(phsA)

SXM:	MKD32_03120(phsA)

SHEJ:

MZ182_02725(phsA)

SSEH:

N7V09_05095(phsA)

SCHK:

GII14_02270(phsA)

SAEG:

K0H80_03080(phsA)

SDEO:

D0436_19415(phsA)

SMES:

K0I73_16585(phsA)

SHAO:

K0H81_03170(phsA)

SZH:	K0H63_17370(phsA)

SSPA:

K0I31_03105(phsA)

SHNS:

K0J45_03100(phsA)

SRHS:

K0I63_03125(phsA)

SPSJ:

K0I62_03005(phsA)

SMAY:

K0H60_18130(phsA)

SOG:	RA178_02865(phsA)

SPSR:

EGC80_16480(phsA)

SHEA:

GUY17_10470(phsA)

SHEL:

JM642_03015(phsA)

SHEH:

ABIS04_09645(phsA)

SYOU:

RGE70_14000(phsA) RGE70_16200(phsA)

FBL:

Fbal_3636

FES:	HER31_12320(phsA)

MDN:	JT25_008470

TUN:	J9260_10030

TLO:	J9253_04310

TSB:	HMY34_18450

TANI:

J8380_14995

TLC:	RCF98_16650

TSJ:	L3K52_10845

TDU:	QJT80_04400

TTP:	E6P07_03930

CJAP:

GWK36_03095

AEH:	Mlg_0633 Mlg_2426

HHA:

Hhal_1166

EBS:	ECTOBSL9_2837(arxA)

TNI:	TVNIR_0949(phsA_[H])

TTI:	THITH_03005

NAX:	HC341_06630

EPS:	L0Y14_05615

SEDS:

AAY24_06365 AAY24_12230

HCO:	LOKO_02585(psrA)

HAAH:

HALA3H3_p20057

KUS:	B9G99_05350

KMA:	B9H00_09830

MASS:

CR152_08555

MANC:

IV454_14530

MFLA:

GO485_03455

MPLI:

E1742_13795

TCT:	PX653_18995

PLUE:

EWM63_06845

SUTT:

SUTMEG_11480 SUTMEG_15000

SUTK:

FG381_02115

SWS:	I6J16_05350

BBAY:

A4V04_07945

SHD:	SUTH_00426

NPV:	OHM77_03730

SLAC:

SKTS_23020

ARES:

IWH25_01975

EBA:

ebA1017

RBH:	B4966_04570 B4966_04605

AZA:	AZKH_p0053

AZI:	AzCIB_1147

ACR:

Acry_0345

AMV:	ACMV_03760(psrA)

VSU:	P3M64_10425

HTL:

HPTL_0252

HWI:	A0Z60_06255(phsA)

HPUL:

NCTC13154_00091(psrA)

WSU:	WS0116(fdhA) WS0709(fdhA)

SUA:	Saut_0756 Saut_0759

SULN:

FJR47_09570

SULC:

CVO_09640

CCV:	CCV52592_1914

CCO:	CCC13826_0389

CCOC:

CCON33237_1350

CAJ:	CIG1485E_0644

CSM:	CSUB8521_1325

CGRA:

CGRAC_0560

CURE:

CUREO_1061

CHYO:

CHH_0519

CHV:	CHELV3228_0175

CSPF:

CSF_0471

CPIN:

CPIN18020_0457

CRX:	CRECT_0711

CGEO:

CGEO_1111

CBLA:

CBLAS_1021

CCOR:

CCORG_0662

CMUC:

CMCT_0504

CSHO:

CSHOW_1391

CNV:	CNZW441b_1003

CCAQ:

CCAL_1387

SDL:	Sdel_0265 Sdel_0269 Sdel_2276

SBA:	Sulba_0281 Sulba_0285 Sulba_2546

SMUL:

SMUL_0342(psrA) SMUL_0346 SMUL_3275 SMUL_3282

SHAL:

SHALO_0307 SHALO_0311 SHALO_2994 SHALO_3001

SULS:

Sdiek1_0301 Sdiek1_0305 Sdiek1_2974 Sdiek1_2981

SULJ:

SJPD1_0305 SJPD1_0309 SJPD1_2864 SJPD1_2871

SULT:

FA592_07095 FA592_08605(phsA) FA592_08625(phsA)

AHS:	AHALO_0201 AHALO_2400

AMYT:

AMYT_1905

AMAR:

AMRN_0240 AMRN_2501

ACAA:

ACAN_0252 ACAN_2514

AMOL:

AMOL_1120 AMOL_1900

HEBR:

AEBR_0305 AEBR_1184

AANA:

AANAER_0294

HTM:	RZR91_12110 RZR91_12125

HCJ:	HCR_12220 HCR_13390

NSA:	Nitsa_1756

SINU:

IMZ28_06785 IMZ28_07170 IMZ28_07270

SMAB:

LN246_00040

NAM:

NAMH_1518

NAP:	C3L23_07885

CMED:

FE773_08475

CPAF:

C6V80_01970

DAV:	DESACE_06425

GEV:	GSVR_02720

GLO:

Glov_1164

TAMM:

GEAMG1_1160

PEF:	A7E78_03700

DEU:

DBW_0837

DVE:	DESUT3_07870

DES:	DSOUD_0599

DDS:

Ddes_1667

DPG:	DESPIGER_0221

DEF:	CNY67_04550

DFL:	DFE_0077 DFE_0136

PSYF:

N1030_03730 N1030_03750

DSB:	LN040_01670 LN040_01690

DMA:	DMR_08220(phaA)

DCB:	C3Y92_16355

DGG:

DGI_0241

DDE:	Dde_0682 Dde_1076

DMS:	E8L03_06385

DSA:	Desal_0371

DAF:	Desaf_1489 Desaf_1628 Desaf_3550

DAS:	Daes_1184 Daes_3179

DPI:

BN4_20079

DEJ:	AWY79_01170 AWY79_07465

PPRF:

DPRO_1730 DPRO_3417

PSEL:

GM415_04510 GM415_09450

DDN:	DND132_0424 DND132_1314 DND132_2597

PSEF:

PSDVSF_12760 PSDVSF_25560

PPOR:

JCM14722_09970 JCM14722_11410 JCM14722_20920

PNW:	SYK_14370 SYK_14810 SYK_22390 SYK_26190

PTHE:

LF599_01615 LF599_07235 LF599_13910

PMEN:

V8V93_04345 V8V93_16970

DSX:	GD604_01660 GD604_16610

DSD:	GD606_10630 GD606_17415

PBIZ:

LWC08_08725

DVU:

DVU_0173

DVL:

Dvul_2794

DVM:	DvMF_0166 DvMF_0320

DVG:	Deval_0198

CLIH:

KPS_000190 KPS_000421

DBA:	Dbac_0171 Dbac_2338 Dbac_2860

DESU:

NLA06_10375 NLA06_12095 NLA06_14260

THEW:

TDMWS_05900

DRT:

Dret_0895

DSF:	UWK_01205 UWK_02642 UWK_02791

DAK:	DaAHT2_0420 DaAHT2_0547

DPR:	Despr_1135 Despr_1859 Despr_2399

DOG:	HP555_09720 HP555_14005

DBK:	DGMP_14050 DGMP_21170 DGMP_32000

DDU:	GF1_06580 GF1_10120 GF1_30490

ECOM:

QTN59_06890 QTN59_13425

EAJ:	Q3M24_05095 Q3M24_16985

ELAX:

KKHLCK_05770 KKHLCK_13045

EAQ:	LDFHOB_07320 LDFHOB_12305

DOL:

Dole_0126

DAL:	Dalk_1125 Dalk_2525

DTO:	TOL2_C03980(phsA)

DOV:	DSCO28_04200 DSCO28_16650 DSCO28_54490

DWD:	DSCW_28610 DSCW_31190

DALK:

DSCA_26800 DSCA_49610 DSCA_57040 DSCA_61680

DML:	Dmul_02650(phsA)

DMM:	dnm_034970

SAT:

SYN_01672

DTI:	Desti_1387 Desti_1738

SFU:	Sfum_1318 Sfum_3287

DAX:	FDQ92_10105

DBR:

Deba_2842

DMP:	FAK_31500 FAK_38620

TID:	Thein_1622

TOP:	TOPB45_0897

TCM:	HL41_04470 HL41_08650

THET:

F1847_03060 F1847_07855

CTHI:

THC_0921

TAV:	G4V39_07125 G4V39_08920

TMAI:

FVE67_02275

ADE:

Adeh_2632

ACP:	A2cp1_2813

AFW:	Anae109_4110

ANK:	AnaeK_2718

AORY:

AMOR_50280 AMOR_50350

APAU:

AMPC_22560

TNR:	Thena_1758

TACI:

TDSAC_1714

OTE:

Oter_1466

AAQW:

K1X11_016010

OBA:	ASA1KI_28040

GEX:	GETHOR_22510

GET:	QOZ81_002531

MSIL:

METEAL_26570

MSEA:

METESE_23180

DRC:	G0Q07_19410

MCOS:

GM418_06250

ANF:

AQPE_4496

MBAS:

ALGA_3219

CLIT:

OQ292_27050

FBC:	FB2170_11556

LUT:	Lupro_00130

SABU:

MBM09_11180

LUY:	QZH61_10475

AEST:

RBH94_06210

MANG:

RBH95_01365

SHYR:

LA303_10780

CCH:

Cag_0620

CPH:	Cpha266_2562

CPB:	Cphamn1_2386

CLI:

Clim_2400

PPH:

Ppha_0912

PAA:

Paes_2139

PROC:

Ptc2401_02170(psrA)

PRS:	B9H02_10550

PROS:

CHL67_11175

PROW:

OO006_01090

PROI:

OO005_01095

IAL:

IALB_1663

MRO:

MROS_1774

DIN:	Selin_2609

DDF:	DEFDS_0670 DEFDS_1699

DAP:	Dacet_1678 Dacet_1793

GTL:	EP073_09415 EP073_11320

CNI:	Calni_0185

FSI:	Flexsi_1504 Flexsi_1512

CABY:

Cabys_1269

TYE:	THEYE_A1128 THEYE_A1157

TOB:	V4D31_04890 V4D31_05390

TAUT:

V4D30_02210 V4D30_02810

DHR:	LGS26_06635 LGS26_09210

HNZ:	P9989_19450

BBEV:

BBEV_1018(psrA-1)

BSE:

Bsel_1476

MJO:	FOF60_05470(srrA) FOF60_09180

MSUT:

LC048_12440 LC048_16385(srrA)

CCE:

Ccel_1634

STH:

STH109

CMIC:

caldi_16080 caldi_16110

DSY:

DSY3896

DHD:

Dhaf_1509

DDH:	Desde_1410

DDL:	Desdi_1252 Desdi_2963

DMT:	DESME_05785

DOR:	Desor_3577

DAI:	Desaci_3271

DCA:	Desca_0977

DKU:	Desku_2752

AACX:

DEACI_1081 DEACI_3149

DGI:	Desgi_1689

CTHM:

CFE_0713 CFE_1029

CHY:

CHY_2574

MTA:

Moth_0450

MTHO:

MOTHE_c03860(psrA)

MTHZ:

MOTHA_c04750(psrA)

MHUI:

MHLNE_04040(phsA)

SACV:

SPACI_037480(psrA)

LPIL:

LIP_1347

CAER:

CSV91_01930

CCU:	Ccur_02090

ELE:	Elen_0442 Elen_0447 Elen_2753 Elen_2919

EYY:	EGYY_22930

EGD:	GS424_002570 GS424_002595 GS424_016830

GPA:

GPA_25490

AEQ:	AEQU_0172 AEQU_0395

AHAT:

ADCFC_03680 ADCFC_20030

DDT:	AAY81_08500

CBAC:

JI75_05060 JI75_08700

BWA:	HLV38_01315 HLV38_05530

RTS:	CE91St31_28640 CE91St31_29860

SPAC:

B1H29_02555

CAP:	CLDAP_25520(phaA)

THUG:

KNN16_09725

TTH:

TT_C0155

TSC:	TSC_c06950

THC:	TCCBUS3UF1_7880

TOS:	Theos_0751

TBC:	A0O31_01685

OPR:	Ocepr_1002

FSH:	Q2T83_17815

HALH:

HTSR_1661

HHSR:

HSR6_1729

HSU:	HLASF_1287

HSF:	HLASA_1275

HAHS:

HSRCO_0103(bisC) HSRCO_1045(bisC5)

SAIM:

K0C01_00030

HARA:

AArcS_0641(bisC) AArcS_0990(bisC2)

HSN:	DV733_07300

HDS:	HSR122_1817(bisC) HSR122_2527(bisC2) HSR122_2992(bisC4)

HDF:	AArcSl_0127(phsA) AArcSl_1905(phsA2)

NGE:	Natgr_0551 Natgr_2276

NAG:	AArcMg_2662

NAN:	AArc1_1042

» show all

Reference

1 [PMID:6384182]

Authors

Kwan HS, Barrett EL

Title

Map locations and functions of Salmonella typhimurium men genes.

Journal

J Bacteriol 159:1090-2 (1984)
DOI:10.1128/JB.159.3.1090-1092.1984

Reference

2 [PMID:3108233]

Authors

Clark MA, Barrett EL

Title

The phs gene and hydrogen sulfide production by Salmonella typhimurium.

Journal

J Bacteriol 169:2391-7 (1987)
DOI:10.1128/JB.169.6.2391-2397.1987

Reference

3 [PMID:7737516]

Authors

Alami N, Hallenbeck PC

Title

Cloning and characterization of a gene cluster, phsBCDEF, necessary for the production of hydrogen sulfide from thiosulfate by Salmonella typhimurium.

Journal

Gene 156:53-7 (1995)
DOI:10.1016/0378-1119(94)00930-Q

Sequence

[stm:STM2065 STM2064 STM2063]

Reference

4 [PMID:7751291]

Authors

Heinzinger NK, Fujimoto SY, Clark MA, Moreno MS, Barrett EL

Title

Sequence analysis of the phs operon in Salmonella typhimurium and the contribution of thiosulfate reduction to anaerobic energy metabolism.

Journal

J Bacteriol 177:2813-20 (1995)
DOI:10.1128/JB.177.10.2813-2820.1995