KEGG   ENZYME: 1.8.99.5Help
Entry
EC 1.8.99.5                 Enzyme                                 

Name
dissimilatory sulfite reductase;
siroheme sulfite reductase;
hydrogen-sulfide:(acceptor) oxidoreductase (ambiguous);
DsrAB
Class
Oxidoreductases;
Acting on a sulfur group of donors;
With unknown physiological acceptors
BRITE hierarchy
Sysname
hydrogen-sulfide:[DsrC sulfur-carrier protein],acceptor oxidoreductase
Reaction(IUBMB)
(1) hydrogen sulfide + a [DsrC protein]-disulfide + 2 acceptor + 3 H2O = sulfite + a [DsrC protein]-dithiol + 2 reduced acceptor + 2 H+ (overall reaction) [RN:R00861];
(1a) hydrogen sulfide + a [DsrC protein]-disulfide = a [DsrC protein]-S-sulfanyl-L-cysteine;
(1b) a [DsrC protein]-S-sulfanyl-L-cysteine + 2 acceptor + 3 H2O = sulfite + a [DsrC protein]-dithiol + 2 reduced acceptor + 2 H+;
(2) a [DsrC protein]-S-sulfanyl-L-cysteine + 3 acceptor + 3 H2O = sulfite + a [DsrC protein]-disulfide + 3 reduced acceptor + 2 H+ (overall reaction);
(2a) a [DsrC protein]-S-sulfanyl-L-cysteine + 3 acceptor + 3 H2O = a [DsrC]-S-sulfo-L-cysteine + 3 reduced acceptor + H+;
(2b) a [DsrC]-S-sulfo-L-cysteine = sulfite + a [DsrC protein]-disulfide
Reaction(KEGG)
R00861;
(other) R00295 R08035
Show
Substrate
hydrogen sulfide [CPD:C00283];
[DsrC protein]-disulfide;
acceptor [CPD:C00028];
H2O [CPD:C00001];
[DsrC protein]-S-sulfanyl-L-cysteine;
[DsrC]-S-sulfo-L-cysteine
Product
sulfite [CPD:C00094];
[DsrC protein]-dithiol;
reduced acceptor [CPD:C00030];
H+ [CPD:C00080];
[DsrC protein]-S-sulfanyl-L-cysteine;
[DsrC protein]-disulfide;
[DsrC]-S-sulfo-L-cysteine
Comment
Contain siroheme. The enzyme is essential in prokaryotic sulfur-based energy metabolism, including sulfate/sulfite reducing organisms, sulfur-oxidizing bacteria, and organosulfonate reducers. In sulfur reducers it catalyses the reduction of sulfite to sulfide (reaction 1 in the right to left direction), while in sulfur oxidizers it catalyses the opposite reaction (reaction 2 in the left to right direction) [1]. The reaction involves the small protein DsrC, which is present in all the organisms that contain dissimilatory sulfite reductase. During the process an intramolecular disulfide bond is formed between two L-cysteine residues of DsrC. This disulfide can be reduced by a number of proteins including DsrK and TcmB [5]. This enzyme is different from EC 1.8.1.2, assimilatory sulfite reductase (NADPH), and EC 1.8.7.1, assimilatory sulfite reductase (ferredoxin), which are involved in sulfate assimilation.
History
EC 1.8.99.5 created 2015
Pathway
ec00633  Nitrotoluene degradation
ec00920  Sulfur metabolism
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K11180  dissimilatory sulfite reductase alpha subunit
K11181  dissimilatory sulfite reductase beta subunit
Genes
TIG: THII_0611 THII_0612
ALV: Alvin_1251(dsrA) Alvin_1252(dsrB)
TVI: Thivi_0544 Thivi_0545
TMB: Thimo_0143 Thimo_0144
MPUR: MARPU_10910 MARPU_10915
TSY: THSYN_22540 THSYN_22545
AEH: Mlg_1653 Mlg_1654
HHA: Hhal_1951 Hhal_1952
HHK: HH1059_16540(dsrA-2) HH1059_16550(dsrB-1)
TGR: Tgr7_2185 Tgr7_2186
TNI: TVNIR_0859(dsrB_[H]) TVNIR_0860 TVNIR_1766 TVNIR_1767(dsvB_[H])
ACII: C4901_06765(dsrA) C4901_06770(dsrB)
TBN: TBH_C2461(dsrB) TBH_C2462(dsrA)
VOK: COSY_0794(dsrB) COSY_0795(dsrA)
EBH: BSEPE_1260(dsrB) BSEPE_1261(dsrA)
ENM: EBS_1783(dsrB) EBS_1784(dsrA)
SHD: SUTH_01443(dsrB) SUTH_01444(dsrA)
SDR: SCD_n02074(dsrA) SCD_n02717(dsrB) SCD_n02718(dsrA)
DVU: DVU0402(dsvA) DVU0403(dvsB)
DHY: DESAM_20116(dsvB) DESAM_20117(dsvA)
DGG: DGI_0689(dsrB) DGI_0691(dsrA)
DEF: CNY67_09565(dsrA) CNY67_09570(dsrB) CNY67_15020(dsrB) CNY67_15025(dsrA)
DTR: RSDT_0579(dsrB) RSDT_0580(dsrA)
DPI: BN4_12627(dsrA) BN4_12628(dsrB)
PPRF: DPRO_1445(dsvB) DPRO_1446(dsvA)
PSEL: GM415_02165(dsrB) GM415_02170(dsrA)
DRT: Dret_0244(dsrA) Dret_0245(dsrB)
DML: Dmul_28170(dsrB) Dmul_28180(dsrA)
DAT: HRM2_42390(dsrB2) HRM2_42400(dsrA2)
DTO: TOL2_C05570(dsrA) TOL2_C05580(dsrB)
DOV: DSCO28_40940(dsvA) DSCO28_40950(dsvB)
DWD: DSCW_41400(dsvA) DSCW_41410(dsvB)
DAX: FDQ92_05050(dsrB) FDQ92_05055(dsrA)
BLAG: BLTE_30830(dsrB-1) BLTE_30840(dsrA-2)
MGY: MGMSRv2__3911(dsrB) MGMSRv2__3912(dsrA)
MGRY: MSR1_25020(dsvA) MSR1_25030(dsvB)
MAGX: XM1_4244(dsrA) XM1_4245(dsrB)
MAGQ: MGMAQ_2110(dsrA) MGMAQ_2111(dsrB)
DSY: DSY0309(dsrA) DSY0310(dsrB)
CHY: CHY_2409(dsrB) CHY_2410(dsrA)
CTE: CT0852(dsrA-1) CT0853(dsrB-1) CT2249(dsrA-2)
PROC: Ptc2401_00028(dsvA) Ptc2401_00029(dsvB)
PROS: CHL67_00155(dsrA) CHL67_00160(dsrB)
TYE: THEYE_A1994(dsrA) THEYE_A1995(dsrB)
THET: F1847_01210(dsrA) F1847_01215(dsrB)
TTN: TTX_0423(dsrA) TTX_0424(dsrA) TTX_0426(dsrA) TTX_0427(dsrA) TTX_1187(dsrB) TTX_1188(dsrA)
 » show all
Taxonomy
Reference
1
  Authors
Schedel, M., Vanselow, M. and Trueper, H. G.
  Title
Siroheme sulfite reductase from Chromatium vinosum. Purification and investigation of some of its molecular and catalytic properties.
  Journal
Arch Microbiol 121:29-36 (1979)
Reference
2  [PMID:7338517]
  Authors
Seki Y, Sogawa N, Ishimoto M
  Title
Siroheme as an active catalyst in sulfite reduction.
  Journal
J Biochem 90:1487-92 (1981)
Reference
3  [PMID:9695921]
  Authors
Pott AS, Dahl C.
  Title
Sirohaem sulfite reductase and other proteins encoded by genes at the dsr locus of Chromatium vinosum are involved in the oxidation of intracellular sulfur.
  Journal
Microbiology 144 ( Pt 7):1881-94 (1998)
DOI:10.1099/00221287-144-7-1881
  Sequence
Reference
4  [PMID:18829451]
  Authors
Oliveira TF, Vonrhein C, Matias PM, Venceslau SS, Pereira IA, Archer M
  Title
The crystal structure of Desulfovibrio vulgaris dissimilatory sulfite reductase bound to DsrC provides novel insights into the mechanism of sulfate respiration.
  Journal
J Biol Chem 283:34141-9 (2008)
DOI:10.1074/jbc.M805643200
Reference
5  [PMID:24662917]
  Authors
Venceslau SS, Stockdreher Y, Dahl C, Pereira IA
  Title
The "bacterial heterodisulfide" DsrC is a key protein in dissimilatory sulfur metabolism.
  Journal
Biochim Biophys Acta 1837:1148-64 (2014)
DOI:10.1016/j.bbabio.2014.03.007
Other DBs
ExplorEnz - The Enzyme Database: 1.8.99.5
IUBMB Enzyme Nomenclature: 1.8.99.5
ExPASy - ENZYME nomenclature database: 1.8.99.5
BRENDA, the Enzyme Database: 1.8.99.5
CAS: 37256-51-2

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