Entry
Name
polyprenyldihydroxybenzoate methyltransferase;
3,4-dihydroxy-5-hexaprenylbenzoate methyltransferase;
dihydroxyhexaprenylbenzoate methyltransferase;
COQ3 (gene name);
Coq3 O-methyltransferase;
DHHB O-methyltransferase
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
BRITE hierarchy
Sysname
S-adenosyl-L-methionine:3,4-dihydroxy-5-all-trans-polyprenylbenzoate 3-O-methyltransferase
Reaction(IUBMB)
S-adenosyl-L-methionine + 3,4-dihydroxy-5-all-trans-polyprenylbenzoate = S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-5-all-trans-polyprenylbenzoate [RN:
R08771 ]
Reaction(KEGG)
Substrate
S-adenosyl-L-methionine [CPD:
C00019 ];
3,4-dihydroxy-5-all-trans-polyprenylbenzoate [CPD:
C17554 ]
Product
S-adenosyl-L-homocysteine [CPD:
C00021 ];
3-methoxy-4-hydroxy-5-all-trans-polyprenylbenzoate [CPD:
C17559 ]
Comment
This enzyme is involved in ubiquinone biosynthesis. Ubiquinones from different organisms have a different number of prenyl units (for example, ubiquinone-6 in Saccharomyces, ubiquinone-9 in rat and ubiquinone-10 in human), and thus the natural substrate for the enzymes from different organisms has a different number of prenyl units. However, the enzyme usually shows a low degree of specificity regarding the number of prenyl units. For example, the human COQ3 enzyme can restore biosynthesis of ubiquinone-6 in coq3 deletion mutants of yeast [3]. The enzymes from yeast and rat also catalyse the methylation of 3-demethylubiquinol-6 and 3-demethylubiquinol-9, respectively [2] (this activity is classified as EC
2.1.1.64 , 3-demethylubiquinol 3-O-methyltransferase).
History
EC 2.1.1.114 created 1999
Pathway
ec00130 Ubiquinone and other terpenoid-quinone biosynthesis
ec01110 Biosynthesis of secondary metabolites
Orthology
K00591 polyprenyldihydroxybenzoate methyltransferase / 3-demethylubiquinol 3-O-methyltransferase
Genes
MMUR : 105856863 105874007(COQ3)
CCAR : 109106117 109106591
CAUA : 113100084 113116766
CGIB : 127974754 128030553
MASI : 127453329 127454357
SASA : 106584027(COQ3) 106605038
STRU : 115175724 115177398
OMY : 110490015 110519152(coq3)
OKE : 118364192 118367269(coq3)
OCLA : 139367166 139394807
SALP : 111959183 111965300
SNH : 120031501(coq3) 120048261
CCLU : 121555950 121585892
PSPA : 121316246 121317373(coq3)
ARUN : 117296197 117296374
AFIL : 140144046 140156354
DSI : Dsimw501_GD21650(Dsim_GD21650)
CCAT : 101449981 101455379
SCAC : 106081742 106081743 106081744
CLON : 129608271 129612915
ACOZ : 120957077 120958582
AARA : 120901876 120901877
AMER : 121599364 121599656
ASTE : 118506042 118506043 118516405 118516409
AMAP : 126556244 126556254
AFUN : 125769665 125769666
AMOU : 128296795 128298315
AMRS : 128707016 128709070
AALI : 118463113 118463621
ACOS : 131259204 131271924
ASUA : 134219943 134219951
CPII : 120414085 120416201
LLL : 129789216 129794278 129796320
MPHA : 105828156 105835080
ACEP : 105619456 105620929 105624786
PBAR : 105430215 105434350
COBS : 139103358 139107902(Coq3)
TLN : 139816139 139818129(Coq3)
VCRB : 124424360 124427022
LHT : 122502722 122502767 122502768
TMOL : 138123061 138123444 138124413
PPYR : 116158620 116158621 116158623 116158669 116159764 116160629 116161196 116163297 116163298 116166495 116166496 116166497 116170871 116173439 116173731 116173732 116174772 116175380 116176992 116177090 116177091 116179134 116179541 116179799 116180872 116180895 116182668
BANY : 112048978 112048979 112048980 112048999 112053655 112053656 112054749 112057925
MJU : 123869453 123869751 123869758 123869759 123870401
BPHI : 137918532 137933778
PRAP : 110993192 110999904 110999906 111004477 111004478
PBX : 123708366 123713357 123718905 123718912
PNAP : 125051221 125052356 125059944
ZCE : 119833841 119833849 119833883 119834005 119834020
CCRC : 123696574 123696575 123696576 123696577 123696578
LSIN : 126975353 126975354
AAGE : 121733787 121733788
LGLY : 125229409 125229913
PAME : 138705917 138714984 138714985 138714986
SGRE : 126323248 126336759
DPX : DAPPUDRAFT_204530 DAPPUDRAFT_304225
PMEO : 129582614 129593712 129599930
CEL : CELE_Y57G11C.11(coq-3)
CBR : CBG_21513(Cbr-coq-3)
PVUL : 126811638 126819444
LSAX : 138962301 138962331
CSAT : 104700381 104749784
RARG : 115755390 115755432
QSA : O6P43_013766 O6P43_021109
RCN : 112177865 112188555 112196988
MSYL : 126612054 126634126
PCOX : 137708634 137740845
MESC : 110605603 110627749
SSPL : 121743144 121759663 121811274
SHIS : 125188019 125196171
PSOM : 113287462 113309652
TAES : 123151503 123160250 123165173
LPER : 127311244 127311806
ZMA : 100272292 103635598 109941453 109941456
MFLO : 136519892 136522058 136528212
SITA : 101762675 101778786
PVIR : 120649130 120670471 120687479 120702650
PHAI : 112873851 112891203
PAUA : 133926258 133930847
ACAK : 137938982 137960258
CGR : 2888978(GVI51_I07425)
NCS : NCAS_0B04170(NCAS0B04170)
NDI : NDAI_0G04260(NDAI0G04260)
TPF : TPHA_0A00670(TPHA0A00670)
TBL : TBLA_0B02330(TBLA0B02330)
TDL : TDEL_0D05250(TDEL0D05250)
KAF : KAFR_0C01400(KAFR0C01400)
KNG : KNAG_0K00720(KNAG0K00720)
LBG : 92209433(LODBEIA_P42370)
CAL : CAALFM_C601840CA(COQ3)
ASAU : 88175525(PUMCH_004465)
YLI : 2907025(YALI2_C00448g)
NTE : NEUTE1DRAFT69561(NEUTE1DRAFT_69561)
SMP : 10808182(SMAC4_07908)
RTHE : 98128730(VTJ83DRAFT_7281)
TATV : 25780307(TrAtP1_008659)
TASP : 36607815(TrAFT101_002770)
PLJ : 28884828(PLICBS_006036)
CDET : 87945257(CDEST_08754)
BFU : BCIN_08g04760(Bccoq3)
PTRR : 6338331(PtrM4_020950)
CDEC : 89991595(IAS62_004824)
CTEG : 91991952(I308_105096)
CBAS : 91985494(I312_105449)
CDEP : 91090449(L203_106241)
KMG : 30162005(I203_107439)
KNE : 92181973(IAR55_004715)
KDJ : 28965744(I303_101819)
KBI : 30208388(I302_100615)
KPIN : 30174728(I206_101672)
KSH : 87954030(IL334_001899)
KER : 91104772(V865_005971)
CCAC : CcaHIS019_0608260(COQ3)
ABP : AGABI1DRAFT118851(AGABI1DRAFT_118851)
ABV : AGABI2DRAFT205921(AGABI2DRAFT_205921)
SCM : SCHCO_02495421(SCHCODRAFT_02495421)
SGRA : EX895_000228 EX895_005689
» show all
Taxonomy
Reference
Authors
Clarke CF, Williams W, Teruya JH.
Title
Ubiquinone biosynthesis in Saccharomyces cerevisiae. Isolation and sequence of COQ3, the 3,4-dihydroxy-5-hexaprenylbenzoate methyltransferase gene.
Journal
J Biol Chem 266:16636-44 (1991)
Sequence
Reference
Authors
Poon WW, Barkovich RJ, Hsu AY, Frankel A, Lee PT, Shepherd JN, Myles DC, Clarke CF
Title
Yeast and rat Coq3 and Escherichia coli UbiG polypeptides catalyze both O-methyltransferase steps in coenzyme Q biosynthesis.
Journal
Sequence
Reference
Authors
Jonassen T, Clarke CF
Title
Isolation and functional expression of human COQ3, a gene encoding a methyltransferase required for ubiquinone biosynthesis.
Journal
Sequence
Reference
Authors
Xing L, Zhu Y, Fang P, Wang J, Zeng F, Li X, Teng M, Li X
Title
Crystallization and preliminary crystallographic studies of UbiG, an O-methyltransferase from Escherichia coli.
Journal
Other DBs
ExPASy - ENZYME nomenclature database: 2.1.1.114