| Entry |
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| Name |
5-methyltetrahydropteroyltriglutamate---homocysteine S-methyltransferase;
tetrahydropteroyltriglutamate methyltransferase;
homocysteine methylase;
methyltransferase, tetrahydropteroylglutamate-homocysteine transmethylase;
methyltetrahydropteroylpolyglutamate:homocysteine methyltransferase;
cobalamin-independent methionine synthase;
methionine synthase (cobalamin-independent);
MetE
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| Class |
Transferases;
Transferring one-carbon groups;
Methyltransferases
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| Sysname |
5-methyltetrahydropteroyltri-L-glutamate:L-homocysteine S-methyltransferase
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| Reaction(IUBMB) |
5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine [RN: R04405]
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| Reaction(KEGG) |
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| Substrate |
5-methyltetrahydropteroyltri-L-glutamate [CPD: C04489];
L-homocysteine [CPD: C00155]
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| Product |
tetrahydropteroyltri-L-glutamate [CPD: C04144];
L-methionine [CPD: C00073]
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| Comment |
Requires phosphate and contains zinc. The enzyme from Escherichia coli also requires a reducing system. Unlike EC 2.1.1.13, methionine synthase, this enzyme does not contain cobalamin.
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| History |
EC 2.1.1.14 created 1972, modified 2003
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| Pathway |
| ec00270 | Cysteine and methionine metabolism |
| ec01110 | Biosynthesis of secondary metabolites |
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| Orthology |
| K00549 | 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase |
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| Genes |
» show all
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| Reference |
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| Authors |
Guest JR, Friedman S, Foster MA, Tejerina G, Woods DD. |
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| Title |
Transfer of the methyl group from N5-methyltetrahydrofolates to homocysteine in Escherichia coli. |
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| Journal |
Biochem J 92:497-504 (1964) |
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| Reference |
|
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| Authors |
Whitfield CD, Steers EJ Jr, Weisbach H. |
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| Title |
Purification and properties of 5-methyltetrahydropteroyltriglutamate-homocysteine transmethylase. |
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| Journal |
J Biol Chem 245:390-401 (1970) |
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| Reference |
|
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| Authors |
Eichel J, Gonzalez JC, Hotze M, Matthews RG, Schroder J. |
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| Title |
Vitamin-B12-independent methionine synthase from a higher plant (Catharanthus roseus). Molecular characterization, regulation, heterologous expression, and enzyme properties. |
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| Journal |
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| Sequence |
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| Reference |
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| Authors |
Gonzalez JC, Peariso K, Penner-Hahn JE, Matthews RG. |
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| Title |
Cobalamin-independent methionine synthase from Escherichia coli: a zinc metalloenzyme. |
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| Journal |
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| Sequence |
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| Reference |
5 |
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| Authors |
Peariso, K., Goulding, C.W., Huang, S., Matthews, R.G. and Penner-Hahn, J.E. |
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| Title |
Characterization of the zinc binding site in methionine synthase enzymes of Escherichia coli: The role of zinc in the methylation of homocysteine. |
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| Journal |
J Am Chem Soc 120:8410-8416 (1998) |
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| Other DBs |
| ExplorEnz - The Enzyme Database: | 2.1.1.14 |
| ExPASy - ENZYME nomenclature database: | 2.1.1.14 |
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