KEGG   ENZYME: 2.1.1.150Help
Entry
EC 2.1.1.150                Enzyme                                 

Name
isoflavone 7-O-methyltransferase
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
BRITE hierarchy
Sysname
S-adenosyl-L-methionine:hydroxyisoflavone 7-O-methyltransferase
Reaction(IUBMB)
S-adenosyl-L-methionine + a 7-hydroxyisoflavone = S-adenosyl-L-homocysteine + a 7-methoxyisoflavone [RN:R07375]
Reaction(KEGG)
Substrate
S-adenosyl-L-methionine [CPD:C00019];
7-hydroxyisoflavone [CPD:C15615]
Product
S-adenosyl-L-homocysteine [CPD:C00021];
7-methoxyisoflavone [CPD:C15616]
Comment
The enzyme from alfalfa can methylate daidzein, genistein and 6,7,4'-trihydroxyisoflavone but not flavones or flavanones.
History
EC 2.1.1.150 created 2003
Pathway
ec00943  Isoflavonoid biosynthesis
Orthology
K13262  isoflavone-7-O-methyltransferase
Genes
GMX: 100776336 100779638 100791825 100792875 100800021 100800912 100811609 100812688 100813225 100818724 100818947 100819257 100819796 100819853 100819957
PVU: PHAVU_007G114000g PHAVU_008G032600g PHAVU_008G032700g
VRA: 106758582 106759051 106759056 106771418 111241498
VAR: 108323040 108323056
CCAJ: 109795035 109802220 109802855 109802960 109818703
MTR: MTR_1g076930 MTR_1g076940 MTR_4g088340 MTR_4g088345 MTR_7g014510 MTR_7g014570
CAM: 101505606
LJA: Lj1g3v3023790.1(Lj1g3v3023790.1) Lj5g3v1212600.1(Lj5g3v1212600.1)
ADU: 107463257 107464082 107486238 107486358 107486360 107486373 107486439 107486555 107486622 107486623 107486645 107489657
AIP: 107612696 107635412 107635414 107635489 107635490 107635608 107635609 107638164 107638166 107638167 107638188 107644738
 » show all
Taxonomy
Reference
1
  Authors
Edwards, R. and Dixon, R.A.
  Title
Isoflavone O-methyltransferase activities in elicitor-treated cell suspension cultures of Medicago sativa.
  Journal
Phytochemistry 30:2597-2606 (1991)
Reference
2  [PMID:11006341]
  Authors
He XZ, Dixon RA.
  Title
Genetic manipulation of isoflavone 7-O-methyltransferase enhances biosynthesis of 4'-O-methylated isoflavonoid phytoalexins and disease resistance in alfalfa.
  Journal
Plant Cell 12:1689-702 (2000)
Reference
3  [PMID:8951042]
  Authors
He XZ, Dixon RA.
  Title
Affinity chromatography, substrate/product specificity, and amino acid sequence analysis of an isoflavone O-methyltransferase from alfalfa (Medicago sativa L.).
  Journal
Arch Biochem Biophys 336:121-9 (1996)
DOI:10.1006/abbi.1996.0539
  Sequence
Reference
4  [PMID:9484461]
  Authors
He XZ, Reddy JT, Dixon RA.
  Title
Stress responses in alfalfa (Medicago sativa L). XXII. cDNA cloning and characterization of an elicitor-inducible isoflavone 7-O-methyltransferase.
  Journal
Plant Mol Biol 36:43-54 (1998)
  Sequence
Reference
5  [PMID:11752378]
  Authors
Liu CJ, Dixon RA.
  Title
Elicitor-induced association of isoflavone O-methyltransferase with endomembranes prevents the formation and 7-O-methylation of daidzein during isoflavonoid phytoalexin biosynthesis.
  Journal
Plant Cell 13:2643-58 (2001)
Reference
6  [PMID:11224575]
  Authors
Zubieta C, He XZ, Dixon RA, Noel JP.
  Title
Structures of two natural product methyltransferases reveal the basis for substrate specificity in plant O-methyltransferases.
  Journal
Nat Struct Biol 8:271-9 (2001)
DOI:10.1038/85029
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 2.1.1.150
IUBMB Enzyme Nomenclature: 2.1.1.150
ExPASy - ENZYME nomenclature database: 2.1.1.150
BRENDA, the Enzyme Database: 2.1.1.150
CAS: 136111-54-1

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