KEGG ENZYME: 2.1.1.157

ENZYME: 2.1.1.157

Entry

EC 2.1.1.157                Enzyme

Name

sarcosine/dimethylglycine N-methyltransferase;
ApDMT;
sarcosine-dimethylglycine methyltransferase;
SDMT;
sarcosine dimethylglycine N-methyltransferase;
S-adenosyl-L-methionine:N,N-dimethylglycine N-methyltransferase

Class

Transferases;
Transferring one-carbon groups;
Methyltransferases

Sysname

S-adenosyl-L-methionine:sarcosine(or N,N-dimethylglycine) N-methyltransferase [N,N-dimethylglycine(or betaine)-forming]

Reaction(IUBMB)

2 S-adenosyl-L-methionine + sarcosine = 2 S-adenosyl-L-homocysteine + betaine (overall reaction) [RN:R10061];
(1a) S-adenosyl-L-methionine + sarcosine = S-adenosyl-L-homocysteine + N,N-dimethylglycine [RN:R07243];
(1b) S-adenosyl-L-methionine + N,N-dimethylglycine = S-adenosyl-L-homocysteine + betaine [RN:R07244]

Reaction(KEGG)

R07243 R07244 R10061

Substrate

S-adenosyl-L-methionine [CPD:C00019];
sarcosine [CPD:C00213];
N,N-dimethylglycine [CPD:C01026]

Product

S-adenosyl-L-homocysteine [CPD:C00021];
betaine [CPD:C00719];
N,N-dimethylglycine [CPD:C01026]

Comment

Cells of the oxygen-evolving halotolerant cyanobacterium Aphanocthece halophytica synthesize betaine from glycine by a three-step methylation process. The first enzyme, EC 2.1.1.156, glycine/sarcosine N-methyltransferase, leads to the formation of either sarcosine or N,N-dimethylglycine, which is further methylated to yield betaine (N,N,N-trimethylglycine) by the action of this enzyme. Both of these enzymes can catalyse the formation of N,N-dimethylglycine from sarcosine [3]. The reactions are strongly inhibited by S-adenosyl-L-homocysteine.

History

EC 2.1.1.157 created 2005, modified 2010

Pathway

ec00260

Glycine, serine and threonine metabolism

ec01100

Metabolic pathways

Orthology

K18897

sarcosine/dimethylglycine N-methyltransferase

K24071

glycine/sarcosine/dimethylglycine N-methyltransferase

Genes

SALH:

HMF8227_02086(gsmT)

LCD:	clem_04510

TIG:

THII_1899

TMB:	Thimo_1799

MPUR:

MARPU_03460

TEE:

Tel_06785

THIP:

N838_15845

THIM:

KFB96_17235

TLR:	Thiosp_00205

TFRI:

Thiofri_00398

TWG:	Thiowin_05093

HHA:

Hhal_1677

HHK:	HH1059_14690

HHC:	M911_05555

EBS:	ECTOBSL9_2475

TGR:

Tgr7_1180

TKM:

TK90_0180

TNI:	TVNIR_0235

TTI:	THITH_16325

TVR:

TVD_00880

SPIU:

SPICUR_05635

SROS:

BBH56_02800

SHAI:

LMH63_03505

NAX:	HC341_02605

WEZ:	IC757_03180

TTC:	FOKN1_2396

THIC:

TspCOW1_02650

ALCA:

ASALC70_02111

SALN:

SALB1_0940

BDL:

AK34_2248

BPYR:

ABD05_28965

BUG:	BC1001_4028

BGP:	BGL_1c12680

BPLA:

bpln_1g12200

BPX:	BUPH_00610

PARA:

BTO02_25155

APP:	CAP2UW1_2978 CAP2UW1_3536

MCI:	Mesci_0387

MCIC:

A4R28_26430

MOP:	Mesop_2713

MESW:

A9K65_001945

MHUA:

MCHK_0500

MJR:	EB229_14570

MERD:

EB233_27850

NIY:	FQ775_18170

PLA:

Plav_2857

KMN:	HW532_17810

RJG:	CCGE525_02200

FIL:	BN1229_v1_3117

FIY:	BN1229_v1_2803

RHZ:	RHPLAN_16680

RBM:

TEF_00780

LAP:	ACP90_08120

LABR:

CHH27_01290

LABT:

FIU93_13755

LAGG:

B0E33_20175

ACUT:

MRB58_00815

RED:	roselon_03243

RID:	RIdsm_00793

MALU:

KU6B_49020 KU6B_49030

PSAP:

JHX88_08465

RHC:

RGUI_2989

HDH:	G5B40_07345

PBAE:

P8S53_19720

GAK:

X907_0698

SSAA:

RPR59_14230

AAY:

WYH_00220

ELQ:	Ga0102493_112273

SVC:	STVA_54880

DEX:	HWD60_10300

MAGQ:

MGMAQ_3233

THAR:

T8K17_03145

HOH:

Hoch_6747

NTH:	Nther_0676

NCD:	ACONDI_00157

ERZ:	ER308_18520

MSA:	Mycsm_00494

MMI:

MMAR_0861

MMAE:

MMARE11_08120

MLI:	MULP_00886

MPSE:

MPSD_09910

MSHO:

MSHO_26200

MKN:	MKAN_20335

MDX:	BTO20_00935

MSHG:

MSG_01046

MFJ:	MFLOJ_23410

MSTO:

MSTO_27360

MSIM:

MSIM_14570

MLW:	MJO58_05525

MNV:	MNVI_18340

MCOO:

MCOO_34280

MBAI:

MB901379_00735

MSEO:

MSEO_49750

MSPG:

F6B93_03465

MDU:	MDUV_36420

MPSC:

MPSYJ_29420

MPOF:

MPOR_13070

SVL:	Strvi_1838

SRC:	M271_16300

SMAL:

SMALA_5460

SSOI:

I1A49_30960

STRE:

GZL_06301

SLD:

T261_1518

SGS:	AVL59_21380

SALW:

CP975_03075

SNR:	SNOUR_29320

SNW:	BBN63_13005

SAUO:

BV401_31165

SGV:	B1H19_02410

SALF:

SMD44_05398 SMD44_06865

SALJ:

SMD11_6312

SFK:

KY5_7985c

SKA:	CP970_42925

SBAT:

G4Z16_08455

SSPB:

CP982_35650

SHUN:

DWB77_06607

SMOB:

J7W19_32210

SGOB:

test1122_02690

SAOV:

G3H79_06490 G3H79_13800

SNIG:

HEK616_12660

STUB:

MMF93_23910

SENG:

OJ254_16950

SANT:

QR300_14135

SYUN:

MOV08_29300

SOV:	QZH56_13990

SHAU:

K9S39_01935 K9S39_40830

SFP:	QUY26_07530 QUY26_14660

SARM:

DVA86_18535

NPC:	KUV85_07820

NGV:	CDO52_05885

STRR:

EKD16_20715

SAIU:

J4H86_12575

ACTW:

F7P10_26560

SRO:

Sros_5201

SEN:

SACE_3901

SACG:

FDZ84_36205

SVI:	Svir_27050

PAUT:

Pdca_14790

ACTI:

UA75_05195

ACAD:

UA74_05190

PMAD:

BAY61_22795

SACE:

GIY23_10125

ACTY:

OG774_25600

MAU:	Micau_2701

MIL:

ML5_5694

MTUA:

CSH63_11670

MICH:

FJK98_17165

MCAB:

HXZ27_14045

CATI:

CS0771_62280

NHY:	JQS43_05010

SNA:

Snas_2966

AEY:	CDG81_09050

ATQ:	GH723_14055

DSL:	Dacsa_3636

HHG:	XM38_033970

HAO:	PCC7418_3511

ENN:	FRE64_13780

TER:	Tery_2447 Tery_4388

MPRO:

BJP34_03740 BJP34_03745 BJP34_29375

NPY:	NPRO_06340

SMAM:

Mal15_41810

SNEP:

Enr13x_26350

AMUC:

Pan181_00950

CCOP:

Mal65_48970

KST:	KSMBR1_0461

PBU:	L21SP3_00980

PBP:	STSP1_02232

ALUS:

STSP2_01991

VBL:	L21SP4_02070

PRHO:

PZB74_19945

FBC:	FB2170_09406

PROC:

Ptc2401_00945(rebM)

CTS:

Ctha_2187

NALL:

PP769_03315

NNEO:

PQG83_14510

NWT:	NSPWAT_0187

MMH:

Mmah_0525

MHAZ:

BHR79_00795

MPOT:

BKM01_07615

MEV:	Metev_0931

MPL:

Mpal_2451

HALJ:

G9465_06325

HTU:

Htur_0668

HSAL:

JMJ58_05750

LOKI:

Lokiarch_49740 Lokiarch_52720

» show all

Reference

1 [PMID:10896953]

Authors

Nyyssola A, Kerovuo J, Kaukinen P, von Weymarn N, Reinikainen T

Title

Extreme halophiles synthesize betaine from glycine by methylation.

Journal

J Biol Chem 275:22196-201 (2000)
DOI:10.1074/jbc.M910111199

Sequence

[ag:AAF87203 AAF87204]

Reference

2 [PMID:11319079]

Authors

Nyyssola A, Reinikainen T, Leisola M

Title

Characterization of glycine sarcosine N-methyltransferase and sarcosine dimethylglycine N-methyltransferase.

Journal

Appl Environ Microbiol 67:2044-50 (2001)
DOI:10.1128/AEM.67.5.2044-2050.2001

Sequence

[ag:AAF87203]

Reference

3 [PMID:12466265]

Authors

Waditee R, Tanaka Y, Aoki K, Hibino T, Jikuya H, Takano J, Takabe T, Takabe T

Title

Isolation and functional characterization of N-methyltransferases that catalyze betaine synthesis from glycine in a halotolerant photosynthetic organism Aphanothece halophytica.

Journal

J Biol Chem 278:4932-42 (2003)
DOI:10.1074/jbc.M210970200

Sequence

[ag:BAC56940]

Other DBs

ExplorEnz - The Enzyme Database:

2.1.1.157

IUBMB Enzyme Nomenclature:

2.1.1.157

ExPASy - ENZYME nomenclature database:

2.1.1.157

BRENDA, the Enzyme Database:

2.1.1.157

DBGET integrated database retrieval system