KEGG   ENZYME: 2.1.1.169
Entry
EC 2.1.1.169                Enzyme                                 

Name
tricetin 3',4',5'-O-trimethyltransferase;
FOMT;
TaOMT1;
TaCOMT1;
TaOMT2
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
Sysname
S-adenosyl-L-methionine:tricetin 3',4',5'-O-trimethyltransferase
Reaction(IUBMB)
3 S-adenosyl-L-methionine + tricetin = 3 S-adenosyl-L-homocysteine + 3',4',5'-O-trimethyltricetin (overall reaction) [RN:R09679];
(1a) S-adenosyl-L-methionine + tricetin = S-adenosyl-L-homocysteine + 3'-O-methyltricetin [RN:R09612];
(1b) S-adenosyl-L-methionine + 3'-O-methyltricetin = S-adenosyl-L-homocysteine + 3',5'-O-dimethyltricetin [RN:R09613];
(1c) S-adenosyl-L-methionine + 3',5'-O-dimethyltricetin = S-adenosyl-L-homocysteine + 3',4',5'-O-trimethyltricetin [RN:R09678]
Reaction(KEGG)
Substrate
S-adenosyl-L-methionine [CPD:C00019];
tricetin [CPD:C10192];
3'-O-methyltricetin [CPD:C19739];
3',5'-O-dimethyltricetin [CPD:C10193]
Product
S-adenosyl-L-homocysteine [CPD:C00021];
3',4',5'-O-trimethyltricetin [CPD:C19807];
3'-O-methyltricetin [CPD:C19739];
3',5'-O-dimethyltricetin [CPD:C10193]
Comment
The enzyme from Triticum aestivum catalyses the sequential O-methylation of tricetin via 3'-O-methyltricetin, 3',5'-O-methyltricetin to 3',4',5'-O-trimethyltricetin [2].
History
EC 2.1.1.169 created 2010
Orthology
K22439  tricetin 3',4',5'-O-trimethyltransferase
Genes
BDI: 100838607
ATS: 109732671 109771271 109782003
TAES: 100125708 100125743 123043005 123043328 123043331 123050874 123051182 123051185 123063735 123064851 123081042 123115575 123115577 123123472 123126145 123129898 123147308 123147740 123164941 123167493 123168065 123172266 123176812 123179699 123187158 542948 543449 780608
PHAI: 112878702 112878706 112901940 112901941
Reference
1  [PMID:18397325]
  Authors
Kornblatt JA, Zhou JM, Ibrahim RK
  Title
Structure-activity relationships of wheat flavone O-methyltransferase: a homodimer of convenience.
  Journal
FEBS J 275:2255-66 (2008)
DOI:10.1111/j.1742-4658.2008.06377.x
  Sequence
Reference
2  [PMID:16730127]
  Authors
Zhou JM, Gold ND, Martin VJ, Wollenweber E, Ibrahim RK
  Title
Sequential O-methylation of tricetin by a single gene product in wheat.
  Journal
Biochim Biophys Acta 1760:1115-24 (2006)
DOI:10.1016/j.bbagen.2006.02.008
  Sequence
Reference
3  [PMID:19211254]
  Authors
Zhou JM, Seo YW, Ibrahim RK
  Title
Biochemical characterization of a putative wheat caffeic acid O-methyltransferase.
  Journal
Plant Physiol Biochem 47:322-6 (2009)
DOI:10.1016/j.plaphy.2008.11.011
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 2.1.1.169
IUBMB Enzyme Nomenclature: 2.1.1.169
ExPASy - ENZYME nomenclature database: 2.1.1.169
BRENDA, the Enzyme Database: 2.1.1.169

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