KEGG   ENZYME: 2.1.1.184
Entry
EC 2.1.1.184                Enzyme                                 
Name
23S rRNA (adenine2085-N6)-dimethyltransferase;
ErmC' methyltransferase;
ermC methylase;
ermC 23S rRNA methyltransferase;
rRNA:m6A methyltransferase ErmC';
ErmC';
rRNA methyltransferase ErmC'
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
Sysname
S-adenosyl-L-methionine:23S rRNA (adenine2085-N6)-dimethyltransferase
Reaction(IUBMB)
2 S-adenosyl-L-methionine + adenine2085 in 23S rRNA = 2 S-adenosyl-L-homocysteine + N6-dimethyladenine2085 in 23S rRNA [RN:R10716]
Reaction(KEGG)
R10716
Substrate
S-adenosyl-L-methionine [CPD:C00019];
adenine2085 in 23S rRNA
Product
S-adenosyl-L-homocysteine [CPD:C00021];
N6-dimethyladenine2085 in 23S rRNA
Comment
ErmC is a methyltransferase that confers resistance to the macrolide-lincosamide-streptogramin B group of antibiotics by catalysing the methylation of 23S rRNA at adenine2085.
History
EC 2.1.1.184 created 1976 as EC 2.1.1.48, part transferred 2010 to EC 2.1.1.184
Orthology
K00561  23S rRNA (adenine-N6)-dimethyltransferase
Genes
CIEAN232_30680
PCIBF9282_16500
PRGRB151_016370(ermD)
PMPPmu_02850(erm(42))
BTOWQG_970
BTRHF543_22890
BTRAF544_3050
ATGJ4G44_12835(erm)
NMENMB0066(ermC)
VFGC9I84_016
BSUTBSUB_10005
BLHBaLi_c28970(ermD)
BHABH0380
BCLABC3508
OIHOB0921
GMCGY4MC1_0187
PTLAOT13_01950
ASEDIRT44_04780
LYBC3943_14160(erm-23S_rRNA)
VIGBKP57_12950
VPNA21D_00889(ermD)
BTHVCQJ30_01485
GRCGI584_11570(erm(A))
SCIAHUG15_07595(erm)
BOUI5818_12075(erm)
SAUSA0048(ermA) SA0766(ermA) SA1480(ermA) SA1951(ermA) SA2384(ermA)
SAVSAV0052(ermA) SAV1655(ermA)
SAWSAHV_0051(ermA) SAHV_1642(ermA)
SAHSaurJH1_0039 SaurJH1_1747
SAJSaurJH9_0039 SaurJH9_1713
SARSAR0050(ermA1) SAR1735(ermA2)
SAASAUSA300_pUSA0307(ermC)
SUVSAVC_05665
SUKSAA6008_00830(ermA) SAA6008_01623(ermA_1)
SUCECTR2_126
SUTSAT0131_01756
SUDST398NM01_2897
SUWSATW20_00430(ermA1) SATW20_16480(ermA2)
SAUNSAKOR_02720
SAUGSA268_2515
SAUZSAZ172_0044(ermA1) SAZ172_1667(ermA2)
SAUTSAI1T1_2006330(ermA) SAI1T1_2011990(ermA) SAI1T1_2015840(ermA) SAI1T1_2019340(ermA)
SAUJSAI2T2_1006350(ermA) SAI2T2_1012010(ermA) SAI2T2_1015850(ermA) SAI2T2_1019350(ermA)
SAUKSAI3T3_1006340(ermA) SAI3T3_1011990(ermA) SAI3T3_1015840(ermA) SAI3T3_1019340(ermA)
SAUQSAI4T8_1006330(ermA) SAI4T8_1012000(ermA) SAI4T8_1015850(ermA) SAI4T8_1019350(ermA)
SAUVSAI7S6_1006340(ermA) SAI7S6_1012010(ermA) SAI7S6_1015850(ermA) SAI7S6_1019340(ermA)
SAUWSAI5S5_1006300(ermA) SAI5S5_1011960(ermA) SAI5S5_1015790(ermA) SAI5S5_1019280(ermA) SAI5S5_20050(ermC)
SAUXSAI6T6_1006310(ermA) SAI6T6_1011970(ermA) SAI6T6_1015800(ermA) SAI6T6_1019290(ermA)
SAUYSAI8T7_1006340(ermA) SAI8T7_1012000(ermA) SAI8T7_1015830(ermA) SAI8T7_1019320(ermA)
SUYSA2981_0052(ermA) SA2981_1614(ermA)
SAUMBN843_16560
SAUCCA347_1648(ermA5) CA347_49(ermA5)
SAURSABB_02575(ermA1) SABB_05250(ermA2)
SERSERP1220(ermA-1) SERP1343(ermA-2) SERP2510(ermA-3)
SHApSHaeB01(ermC)
SDTSPSE_1800(ermA)
SPETCEP67_02750 CEP67_07740
MLENH3V22_01745(erm(B)) H3V22_01785(erm(B))
MCLMCCL_plsB0042
MACRBHM04_10840
SBACBVH56_08720
EAURNMQ00_15990(erm(B))
GYMGYMC10_1326
PSONJI735_30615
POWIJ21_00020
PIBBBD41_08175
PVOPVOR_08690
PLWD5F53_22980(erm)
PLUTEI981_22720(erm)
NTRB0W44_01260
TVUAB849_011290(erm)
KPULGXN76_07960(erm)
KEBGXN75_04125(erm)
SPIMGAS10750_Spy1703
SPWSPCG_0172 SPCG_1323 SPCG_1327
SPXSPG_1241(ermB)
SPNNT308_09065
SPVSPH_1420
SNCHMPREF0837_12197
SNBSP670_1166
SNPSPAP_0896(erm(TR))
SAGEEN72_06990
SSBSSUBM407_0952(ermB)
SSUSNJAUSS_0669
SSKSSUD12_1346 SSUD12_1565
SSQSSUD9_1049
SUISSUJS14_0695
SUOSSU12_0879
SORSOR_1880(ermB)
STDSPPN_01485
SIUSII_0641(ermB)
SANCSANR_1363(ermTR)
SOII872_07900
SLUKE3_0212
SEQIA6J79_00310
SGWD7D53_04400(erm(B))
SPABKQ224_08640(erm(B))
LJHLJP_0027c
LAILAC30SC_10855
LAHLA20533_04015
EFAEFA0007
EFDEFD32_pA0006
EFCEFAU004_01593(ermG)
EFAUEFAU085_p3012(erm)
EFUHMPREF0351_12804(ermA)
EAVEH197_00455(erm(B))
ESGEsVE80_07090(ermB) EsVE80_p1-00130(ermB)
ECECNCTC12421_01166(erm)
ERAFJ9537_20525(erm(B))
THLTEH_23120
TKRC7K43_00275(erm-23S_rRNA)
VACE4Z98_09655(erm(B))
VFVK5K99_14655(erm(B))
AURHMPREF9243_0168
ABAECL176_05680
JARG7057_06385(erm(B))
CIABEN51_12270
AOEClos_2851
HSCHVS_13755(ermA1)
RUKA4V00_10655
CTHDCDO33_12400
VFAMM35RIKEN_22660(ermB)
VCOPMM50RIKEN_19590(ermB)
HSDSD1D_1531
CDFCD630_20070(ermB) CD630_20100(ermB1)
PDCCDIF630_02225(ermB1) CDIF630_02229(ermB2)
PDFCD630DERM_20072(ermB)
PHXKGNDJEFE_00529(erm_1) KGNDJEFE_01831(ermC') KGNDJEFE_02272(erm_2)
DDHDesde_1644 Desde_1647
DFGB0537_11580
EMTCPZ25_016270
VPYHZI73_25260(erm)
FMSM1R53_06000(erm(A))
ETMCE91St48_05660(ermB)
HORHore_16530
HALSD7D81_05390 D7D81_13075(erm)
PEDING2D1G_1534(ermGT)
CADCuri_c11270
KPARJL105_07480(erm)
ECOUM1R54_02815(erm(A)) M1R54_03150(erm(A))
VNKVEIT17_15390(ermB)
SELOAXE86_11765
AARGAargi30884_28740(ermB)
EBMSG0102_07200(ermB)
TURAT726_11135
MTURv1988(erm(37))
MTVRVBD_1988
MTCMT2042
MRAMRA_2001
MTFTBFG_12019
MTBTBMG_02000
MTKTBSG_02011
MTZTBXG_001984
MTGMRGA327_12240
MTIMRGA423_12355
MTECCDC5079_1836
MTURCFBS_2095
MTLCCDC5180_1811
MTOMTCTRI2_2021
MTDUDA_1988
MTNERDMAN_2189
MTJJ112_10605
MTUBMT7199_2017
MTUCJ113_13695
MTUEJ114_10620
MTXM943_10315
MTUHI917_14015
MTULTBHG_01945
MTUTHKBT1_2091
MTUUHKBT2_2093
MTQHKBS1_2096
MBOBQ2027_MB2010(erm(37))
MBBBCG_2004
MBTJTY_1999
MBMBCGMEX_1986
MBKK60_020580
MBXBCGT_1798
MAFMAF_19990
MMICRN08_2199
MCEMCAN_20081
MCQBN44_40271
MCZBN45_50265
MYVG155_08570
MKRMKOR_26660
MSENK3U95_07780(erm)
MSMMSMEG_1646
MSGMSMEI_1608
MFTXA26_17170
MVQMYVA_1303(erm(X))
MCHTMCHIJ_07850
MMATMMAGJ_45650
MBOKMBOE_57580
MABMAB_2297
MPAKMIU77_11050(erm)
CJKjk1436(erm(X))
CURcu1922(erm(X))
CUACU7111_1847(erm(X))
CGYCGLY_13945(ermA)
CDOCDOO_02540
CHMB842_00165
CSXCSING_13175(ermA)
CMVCMUST_04105(ermA)
CFCCFLV_12470
CSTRCBE89_09900
CAMGCAMM_02585
CPRPI6I69_04585(erm)
CGLUI6J20_08505(erm)
CLUJIAU68_04200(erm)
NFANFA_27210
NFRERS450000_01376(rsmA_1)
NCYNOCYR_3185(carB)
NBRO3I_025710 O3I_028830
NNONONO_c69700
NTPCRH09_08515
NOZDMB37_05105(erm)
NODFOH10_07535(erm)
NAHF5544_25790(erm)
NADNCTC11293_06190(ermC')
NWLNWFMUON74_65270
NIEKV110_25405(erm)
NHUH0264_15925(erm)
NYALTV02_34005(erm)
NGPLTT66_28290(erm)
RHUA3Q40_02474(carB_2)
RCRNCTC10994_01728(ksgA_2)
RTMG4H71_02885(erm)
GBRGbro_3423
TPRTpau_0528
TSMASU32_02800
TPULTPB0596_05520
DTMBJL86_0111
DIZCT688_16390
DPCA6048_17085
DKNNHB83_18615(erm)
TOYFO059_06945(erm)
SCOSCO6089(SCBAC1A6.13)
SVLStrvi_2357
SRCM271_13995
SMALSMALA_5962
SSOII1A49_33645(erm)
SBHSBI_03035
SDVBN159_2247(ermSF)
SLVSLIV_07695(ermSF)
SXISXIM_38360
STRCAA958_28040
SAMBSAM23877_5636(srm1) SAM23877_5808(ermSF)
SPAVSpa2297_25715
STSIA4E84_31320
SLSSLINC_0256
STRDNI25_08920
SNWBBN63_06400
SALFSMD44_05855(ksgA)
SALJSMD11_1643
SNZDC008_27420
SGEDWG14_01987(carB_1)
SQZFQU76_07780(erm)
SASTCD934_06025
SNQCP978_26430(erm)
SHAWCEB94_31945
SAQUEJC51_36560(erm)
SPACB1H29_08255
STSUB7R87_07750
SCHACP983_09665(erm)
SGXH4W23_25560 H4W23_25565(erm)
SCOECP976_33775(erm(O))
SBATG4Z16_22520(erm)
SSPBCP982_28015(erm)
SHARHUT13_18995
SFEUIM697_32505(erm)
SVDCP969_29465(erm)
SROIIAG44_08630(erm)
SMAOCAG99_07230
SLFJEQ17_11955(erm)
SAOVG3H79_11460(erm)
MIXAB663_000810
MPALBO218_00270
MWAE4K62_06780(erm)
MSEDE3O41_03110(erm)
MOYCVS54_00826(carB_1)
MLZF6J85_06495(erm)
MCHNHCR76_14615(erm)
MRNK8F61_10335(erm)
MICSC1N74_10880(erm-23S_rRNA)
MBINLXM64_10360(erm)
AARCG127AT_06435(erm)
AMAUDSM26151_27220(rsmA)
ASOIMTP13_06600(erm)
CPHYB5808_18090
LEULeucomu_03315(erm)
LTREVS81_01550(erm)
AGGC1N71_01480(erm)
ARIUM93_13695
GPRJQN66_18405(erm)
NAEBHE16_06395
AULDCC27_002790(erm)
BCVBcav_1923
BRXBH708_17385
BSAUDWV08_00885
BKIM4486_00965(erm)
DJJCOP05_07550
KSEKsed_17860
LMOIVV02_04275
CCEUCBR64_12025
OEKFFI11_009090(erm)
BLINBLSMQ_0384
BLUTEW640_10075(erm)
BCAUI6G59_05385(erm) I6G59_16080
GEZFE251_15160
HALTIM660_17970
RHALLQF10_17760(rlmJ)
RSUALQF12_15200
PPCHMPREF9154_2441
ARUBJ5A65_05065(erm)
PBOPACID_32300
AACIASQ49_06035 ASQ49_16540
MPHMLP_23220
MIKFOE78_01450
MICGGJV80_18770
TDFH9L22_03800(erm)
BRTJ4N02_08095(erm)
NOYEXE57_00825
NANOG5V58_24520
NROK8W59_14555
PSIMKR76_04810
AERAERYTH_15865
MUZH4N58_14510(erm)
KFLKfla_5998
KQIF1D05_14645
THAONI17_017960(erm)
TALXFOF52_15385(erm)
NDANdas_2264
NALB005_4554
NGVCDO52_15325
NEXNE857_15875(erm)
SAIUJ4H86_03440(erm)
TCUTcur_0508
ACTWF7P10_28120(erm)
SROSros_4551
NOABKM31_35115
NOWGBF35_09980(erm)
NCXNocox_09195(carB1)
NGNLCN96_10305(erm)
TBITbis_3490
SENSACE_0733(ermE)
SACGFDZ84_11210(erm)
SVISvir_28640
SACCEYD13_15795(ermD)
AJAAJAP_39985(ermA)
AMQAMETH_5559(ermE)
AMYCCU254_19965
AABA4R43_20630
AMYYYIM_08965(ermD)
AORISD37_02260
AACDLWP59_32270
PSEAWY02_07165
PSEHXF36_07650
PECQAD017_22270
PHHAFB00_22305
PAUTPdca_44150
KALKALB_1436
KPHYAOZ06_12370
AHMTL08_04290
ACTIUA75_04315
ACADUA74_04215
AHGAHOG_04070(carB1)
ACTAC1701_19120(erm-23S_rRNA)
PMADBAY61_24475
SACEGIY23_06515(erm)
ACTUActkin_05847(carB_2)
STPStrop_3669
SAQSare_4045
MTUACSH63_31505
MICHFJK98_27070(erm)
MTEMGCE86_20490(erm)
MCABHXZ27_06190(rosS)
MSAGGCM10017556_14360
AMSAMIS_48830
AFSAFR_03150
ACTRAsp14428_40630
PLKCIK06_23790
PLABC6361_02045
PLATC6W10_19625
PFLAPflav_081920
PSUUPsuf_044740
ATLAthai_06990
ASERAsera_65950
PRYPrubr_69530
DVCDvina_34665(erm)
NHYJQS43_12230
CCAZCOUCH_36525(erm)
CAICaci_6125
SNASnas_2596
NAVJQS30_00520(erm)
GLYK3N28_20195(erm)
SOOFBF35_08030(erm)
FSLEJO69_09850(erm)
BKSBBKW_0982
BCHOBcFMB_02975
GPTK9E43_01345
GSWK9E37_01815
AEYCDG81_11200 CDG81_13135(rsmA)
EKEEK0264_06100
CWOCwoe_0389
ATQGH723_14315
IAMHC251_10005
EYYEGYY_26800(KsgA)
ERZER308_08415
EUZDVS28_a1268
TTRTter_2586
BUNBun01g_12530(ermC)
BEGINE88_02670(ermC)
BSABacsa_2566
PCOPI6J50_02055(erm)
PBTING2E5B_2269(ermF)
PMUCING2E5A_0789(ermFU)
PETPEIBARAKI_5223
PSACPSM36_1528(ermF)
PGOLK6V26_03560(erm(F))
BVSBARVI_12255
COPRCop2CBH44_11580(ermC)
BUYD8S85_06020(erm(B))
ALDGFH31_01555(erm(F))
ACOUA5CBH24_09260
PSNPedsa_1646
SPHEGFH32_09105
OCIFEZ18_11405(erm)
RAGB739_0033
CNREB819_06990(erm)
PTANCRYO30217_03560(ermD)
TMATM1538
TMMTmari_1546
TMITHEMA_06595
TMWTHMA_1572
TMQTHMB_1572
TMXTHMC_1572
TPTTpet_1254
TNPTnap_1270
TRQTRQ2_1201
TNACTN_1121
THQT2812B_06025
THZCELL2_06590
THRTRQ7_07080
TLETlet_0379
TTATheth_1672
PHYAJ81_04635
TMETmel_0667
TAFTHA_907
THPBG95_03920
THERY592_03970
TFEJYK00_01715
FNOFnod_0842
FPEFerpe_1056
FIANA23_05360
OCYOSSY52_00800
MPZMarpi_0993
MARNLN42_05435
DTNDTL3_0384 DTL3_1144
ASACATHSA_0057
SAALL336_0918
SBERAAC3_TM7C01G0614
WWEP147_WWE3C01G0221
MIBUY43_C0001G1083(ine151)
 » show all
Reference
1  [PMID:7543473]
  Authors
Zhong P, Pratt SD, Edalji RP, Walter KA, Holzman TF, Shivakumar AG, Katz L
  Title
Substrate requirements for ErmC' methyltransferase activity.
  Journal
J Bacteriol 177:4327-32 (1995)
DOI:10.1128/JB.177.15.4327-4332.1995
  Sequence
Reference
2  [PMID:2492520]
  Authors
Denoya C, Dubnau D
  Title
Mono- and dimethylating activities and kinetic studies of the ermC 23 S rRNA methyltransferase.
  Journal
J Biol Chem 264:2615-24 (1989)
Reference
3  [PMID:2440853]
  Authors
Denoya CD, Dubnau D
  Title
Site and substrate specificity of the ermC 23S rRNA methyltransferase.
  Journal
J Bacteriol 169:3857-60 (1987)
DOI:10.1128/JB.169.8.3857-3860.1987
Reference
4  [PMID:9585521]
  Authors
Bussiere DE, Muchmore SW, Dealwis CG, Schluckebier G, Nienaber VL, Edalji RP, Walter KA, Ladror US, Holzman TF, Abad-Zapatero C
  Title
Crystal structure of ErmC', an rRNA methyltransferase which mediates antibiotic resistance in bacteria.
  Journal
Biochemistry 37:7103-12 (1998)
DOI:10.1021/bi973113c
  Sequence
Reference
5  [PMID:10366505]
  Authors
Schluckebier G, Zhong P, Stewart KD, Kavanaugh TJ, Abad-Zapatero C
  Title
The 2.2 A structure of the rRNA methyltransferase ErmC' and its complexes with cofactor and cofactor analogs: implications for the reaction mechanism.
  Journal
J Mol Biol 289:277-91 (1999)
DOI:10.1006/jmbi.1999.2788
  Sequence
Reference
6  [PMID:12907737]
  Authors
Maravic G, Bujnicki JM, Feder M, Pongor S, Flogel M
  Title
Alanine-scanning mutagenesis of the predicted rRNA-binding domain of ErmC' redefines the substrate-binding site and suggests a model for protein-RNA interactions.
  Journal
Nucleic Acids Res 31:4941-9 (2003)
DOI:10.1093/nar/gkg666
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 2.1.1.184
IUBMB Enzyme Nomenclature: 2.1.1.184
ExPASy - ENZYME nomenclature database: 2.1.1.184
BRENDA, the Enzyme Database: 2.1.1.184

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