KEGG ENZYME: 2.1.1.225

ENZYME: 2.1.1.225

Entry

EC 2.1.1.225 Enzyme

Name

tRNA:m4X modification enzyme;
TRM13;
Trm13p;
tRNA:Xm4 modification enzyme

Class

Transferases;
Transferring one-carbon groups;
Methyltransferases

Sysname

S-adenosyl-L-methionine:tRNAPro/His/Gly(GCC) (cytidine/adenosine4-2'-O)-methyltransferase

Reaction(IUBMB)

(1) S-adenosyl-L-methionine + cytidine4 in tRNAPro = S-adenosyl-L-homocysteine + 2'-O-methylcytidine4 in tRNAPro;
(2) S-adenosyl-L-methionine + cytidine4 in tRNAGly(GCC) = S-adenosyl-L-homocysteine + 2'-O-methylcytidine4 in tRNAGly(GCC);
(3) S-adenosyl-L-methionine + adenosine4 in tRNAHis = S-adenosyl-L-homocysteine + 2'-O-methyladenosine4 in tRNAHis

Substrate

S-adenosyl-L-methionine [CPD:C00019];
cytidine4 in tRNAPro;
cytidine4 in tRNAGly(GCC);
adenosine4 in tRNAHis

Product

S-adenosyl-L-homocysteine [CPD:C00021];
2'-O-methylcytidine4 in tRNAPro;
2'-O-methylcytidine4 in tRNAGly(GCC);
2'-O-methyladenosine4 in tRNAHis

Comment

The enzyme from Saccharomyces cerevisiae 2'-O-methylates cytidine4 in tRNAPro and tRNAGly(GCC), and adenosine4 in tRNAHis.

History

EC 2.1.1.225 created 2011

Orthology

K15446

tRNA:m4X modification enzyme

Genes

HSA:	54482(TRMT13)

PTR:	742372(TRMT13)

PPS:	100972211(TRMT13)

GGO:	101148351(TRMT13)

PON:	100456893(TRMT13)

NLE:	100580575(TRMT13)

MCC:	711396(TRMT13)

MCF:	102137915(TRMT13)

CSAB:

103224368(TRMT13)

RRO:	104673250(TRMT13)

RBB:	108524171(TRMT13) 108525930

CJC:	100391711(TRMT13)

SBQ:	101047705(TRMT13)

MMU:	229780(Trmt13)

RNO:	499697(Trmt13)

CGE:

100760563

NGI:	103737440 103743348

HGL:	101721354(Trmt13)

CCAN:

109688165(Trmt13)

OCU:	100347251(TRMT13)

TUP:	102488274(TRMT13)

CFA:	479930(TRMT13)

AML:	100480930(TRMT13)

UMR:	103669334(TRMT13)

ORO:	101372186(TRMT13)

FCA:	101086829(TRMT13)

PTG:	102961454(TRMT13)

AJU:	106976410(TRMT13)

BTA:	614311(TRMT13)

BOM:	102272220(TRMT13)

BIU:	109556020(TRMT13)

PHD:	102341222(TRMT13)

CHX:	102177115(TRMT13)

OAS:	101112862(TRMT13)

SSC:	102167932(TRMT13)

CFR:	102516817(TRMT13)

CDK:	105084984(TRMT13)

BACU:

103003812(TRMT13)

LVE:	103086778(TRMT13)

OOR:	101282936(TRMT13)

ECB:	100050460(TRMT13)

EPZ:	103541514(TRMT13)

EAI:	106837784(TRMT13)

MYB:	102251850(TRMT13)

MYD:	102753968(TRMT13)

HAI:	109374612(TRMT13)

RSS:	109455456(TRMT13)

PALE:

102881835(TRMT13)

LAV:	100657527(TRMT13)

TMU:

101348709

MDO:	100032957(TRMT13)

SHR:	100913690(TRMT13)

OAA:	100081774(TRMT13)

GGA:	424470(TRMT13)

MGP:	100550808(TRMT13)

CJO:	107317455(TRMT13)

APLA:

101790226(TRMT13) 106016845

ACYG:

106039449(TRMT13)

TGU:	100230227(TRMT13)

GFR:	102042132(TRMT13)

FAB:	101821407(TRMT13)

PHI:	102105730(TRMT13)

PMAJ:

107208061(TRMT13)

CCW:	104695571(TRMT13)

FPG:	101911980(TRMT13)

FCH:	102055641(TRMT13)

CLV:	102092758(TRMT13)

EGZ:	104134029(TRMT13)

AAM:	106499749(TRMT13)

ASN:	102374605(TRMT13)

AMJ:	102560657(TRMT13)

PSS:	102444671(TRMT13)

CMY:	102929911(TRMT13)

CPIC:

101948973(TRMT13)

ACS:	100563797(trmt13)

PVT:	110076503(TRMT13)

PBI:	103056354(TRMT13)

GJA:	107112252(TRMT13)

XLA:	443770(trmt13.L)

XTR:	100379916(trmt13)

NPR:	108791472(TRMT13)

DRE:	541496(trmt13)

SRX:	107757496(trmt13)

SANH:

107669842(trmt13)

SGH:	107580953 107588056 107592707(trmt13)

CCAR:

109047774 109052833

IPU:	108271181(trmt13)

AMEX:

103035609(trmt13)

TRU:	101064514(trmt13)

TNG:	GSTEN00008272G001

LCO:	104936348(trmt13)

MZE:	101468812(trmt13)

OLA:	101161965(trmt13)

XMA:	102231464(trmt13)

PRET:

103463270(trmt13)

NFU:	107395230(trmt13)

CSEM:

103398595(trmt13)

LCF:	108874169(trmt13)

HCQ:	109506849(trmt13)

BPEC:

110162795(trmt13)

SASA:

106573941(trmt13)

ELS:	105011372(trmt13)

SFM:	108919082(trmt13)

LCM:	102357633(TRMT13)

CMK:	103184743(trmt13)

BFO:	BRAFLDRAFT_285163

SPU:	575954 582163

SKO:	102802454 102802703

DME:	Dmel_CG18048(CG18048)

DPO:	Dpse_GA14776

DAN:	Dana_GF16397

DER:	Dere_GG13358

DPE:	Dper_GL24080

DSE:	Dsec_GM10888

DSI:	Dsimw501_GD19867(Dsim_GD19867)

DWI:	Dwil_GK13057

DYA:	Dyak_GE10212

DGR:	Dgri_GH14041

DMO:	Dmoj_GI24415

DVI:	Dvir_GJ14273

MDE:

101892271

AGA:	AgaP_AGAP000681

AAG:

5574527

CQU:	CpipJ_CPIJ009806

AME:

BIM:

BTER:

SOC:

AEC:

ACEP:

PBAR:

HST:

CFO:

LHU:

PGC:

NVI:

TCA:

DPA:

NVL:

BMOR:

DPL:	KGM_204636

PMAC:

PRAP:

PXY:

ZNE:

FCD:

DPX:	DAPPUDRAFT_313492

ISC:	IscW_ISCW018578

CEL:	CELE_Y49A3A.3(Y49A3A.3)

CBR:

CBG04631

NAI:	NECAME_14528 NECAME_18467

BMY:

Bm1_32655

LOA:	LOAG_00110

HRO:	HELRODRAFT_77676

LGI:	LOTGIDRAFT_102700

CRG:

MYI:

OBI:

LAK:

SMM:	Smp_156030 Smp_196960

SHX:

MS3_00865

OVI:	T265_13063

NVE:	NEMVE_v1g90494

EPA:

110236250

ADF:

107342372

HMG:	100209000 100214801

TAD:	TRIADDRAFT_57802

AQU:	105314577 105314578 105315862 109592739

ATH:

AT4G01880

ALY:	ARALYDRAFT_490389

CRB:

17883442

CSAT:

104707920 104713456 104737600

EUS:	EUTSA_v10028644mg EUTSA_v10028645mg

BRP:

103858799

BNA:	106389094 106438647 111211753

BOE:

THJ:

CPAP:

CIT:

CIC:	CICLE_v10001046mg

TCC:

GRA:

GHI:

DZI:

EGR:	104428969 104456606

GMX:

100793810

PVU:	PHAVU_003G019300g

VRA:

106776974

VAR:

108335159

CCAJ:

109791691

MTR:	MTR_1g032120

CAM:

101513602

ADU:

107482805

AIP:

107638235

LJA:	Lj0g3v0339199.1(Lj0g3v0339199.1) Lj0g3v0339199.2(Lj0g3v0339199.2)

LANG:

FVE:

PPER:

PMUM:

PAVI:

MDM:	103400765 103421931 103444409

PXB:	103953085 103964908

ZJU:

CSV:

CMO:

MCHA:

CMAX:

CMOS:

CPEP:

RCU:

JCU:

HBR:	110640927 110661741

POP:	112324028 7481431

JRE:

VVI:

SLY:

SPEN:

SOT:	102589230 107060134

NTA:	107771521 107824039 107829617

NSY:

104213533

NTO:	104097924 104120316

INI:

SIND:

OEU:

HAN:

LSV:

DCR:

BVG:

SOE:

NNU:

OSA:

DOSA:

Os03t0833200-01(Os03g0833200)

OBR:

102700415

BDI:

100823311

ATS:	109755505(LOC109755505)

SBI:

ZMA:

SITA:

PDA:

EGU:

MUS:

DCT:

AOF:

ATR:

SMO:	SELMODRAFT_125891 SELMODRAFT_427800

PPP:

112274211

CRE:	CHLREDRAFT_151570

VCN:	VOLCADRAFT_117653

OLU:	OSTLU_24121

MIS:	MICPUN_107426(CHP1)

MPP:	MICPUCDRAFT_5259

CSL:	COCSUDRAFT_48390

CVR:	CHLNCDRAFT_52958

APRO:

F751_2767

CCP:	CHC_T00000204001

SCE:	YOL125W(TRM13)

AGO:	AGOS_ADL163W

ERC:

Ecym_2047

KLA:	KLLA0E07547g

KMX:	KLMA_40390(TRM13)

LTH:	KLTH0F18832g

VPO:	Kpol_1076p3

ZRO:	ZYRO0C02640g

CGR:	CAGL0E01903g

NCS:	NCAS_0C04580(NCAS0C04580)

NDI:	NDAI_0G03950(NDAI0G03950)

TPF:	TPHA_0P01340(TPHA0P01340)

TBL:	TBLA_0B08620(TBLA0B08620)

TDL:	TDEL_0E00570(TDEL0E00570)

KAF:	KAFR_0C01700(KAFR0C01700)

PPA:	PAS_chr2-1_0523

DHA:	DEHA2E17446g

PIC:	PICST_32922

PGU:	PGUG_03255

SPAA:

SPAPADRAFT_70629

LEL:	LELG_00231

CAL:	CAALFM_CR00670CA(CaO19.7460)

CTP:	CTRG_01011

COT:	CORT_0A02530

CDU:	CD36_25770

CTEN:

CANTEDRAFT_111474

YLI:	YALI0E27137g

CLU:	CLUG_03052

CLUS:

A9F13_12g00682

CAUR:

QG37_00429

SLB:	AWJ20_904(TRM13)

SPO:	SPAC1805.03c(trm13)

MBR:	MONBRDRAFT_27936

SRE:	PTSG_00880

DDI:	DDB_G0293682

DPP:	DICPUDRAFT_148568

DFA:

DFA_09068

EHI:	EHI_083130(38.t00028)

EDI:	EDI_077820

EIV:	EIN_221870

ACAN:

ACA1_180610

CPV:

cgd1_2810

CHO:	Chro.10316

TET:	TTHERM_00083540

PTM:	GSPATT00016685001

SMIN:

v1.2.021497.t1(symbB.v1.2.021497.t1) v1.2.021499.t1(symbB.v1.2.021499.t1)

PTI:	PHATRDRAFT_47468

FCY:	FRACYDRAFT_260308

TPS:	THAPSDRAFT_5079

NGD:	NGA_2071910 NGA_2071920

PIF:	PITG_04858

PSOJ:

PHYSODRAFT_533448

SPAR:

SPRG_19285

EHX:	EMIHUDRAFT_200603 EMIHUDRAFT_247952

GTT:	GUITHDRAFT_103306 GUITHDRAFT_110392

TBR:	Tb927.4.4030

TCR:

509237.50

LMA:	LMJF_31_0520

LIF:	LINJ_31_0540

LDO:	LDBPK_310540

LMI:	LMXM_30_0520

LBZ:	LBRM_31_0680

» show all

Reference

1 [PMID:17242307]

Authors

Wilkinson ML, Crary SM, Jackman JE, Grayhack EJ, Phizicky EM

Title

The 2'-O-methyltransferase responsible for modification of yeast tRNA at position 4.

Journal

RNA 13:404-13 (2007)
DOI:10.1261/rna.399607

Sequence

[sce:YOL125W]

Other DBs

ExplorEnz - The Enzyme Database:

2.1.1.225

IUBMB Enzyme Nomenclature:

2.1.1.225

ExPASy - ENZYME nomenclature database:

2.1.1.225

BRENDA, the Enzyme Database:

2.1.1.225

All links

Gene (2469)   
   KEGG ORTHOLOGY (1)   
   KEGG GENES (352)   
   KEGG MGENES (189)   
   RefGene (1842)   
   EGENES (85)   
Protein sequence (36)   
   UniProt (16)   
   SWISS-PROT (12)   
   RefSeq(pep) (8)   
DNA sequence (11)   
   RefSeq(nuc) (10)   
   EMBL (1)   
Protein domain (2)   
   InterPro (2)   
All databases (2518)   

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