S-adenosyl-L-methionine + cytidine1409 in 16S rRNA = S-adenosyl-L-homocysteine + 2'-O-methylcytidine1409 in 16S rRNA
cytidine1409 in 16S rRNA
2'-O-methylcytidine1409 in 16S rRNA
The bifunctional enzyme from Mycobacterium tuberculosis 2'-O-methylates cytidine1409 in helix 44 of 16S rRNA and cytidine1920 in helix 69 of 23S rRNA (cf. EC 22.214.171.124, 23S rRNA (cytidine1920-2'-O)-methyltransferase).