ENZYME: 2.1.1.244 Help
Entry
Name
protein N-terminal methyltransferase;
NMT1 (gene name);
METTL11A (gene name)
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
BRITE hierarchy
Sysname
S-adenosyl-L-methionine:N-terminal-(A,P,S)PK-[protein] methyltransferase
Reaction(IUBMB)
(1) 3 S-adenosyl-L-methionine + N-terminal-(A,S)PK-[protein] = 3 S-adenosyl-L-homocysteine + N-terminal-N,N,N-trimethyl-N-(A,S)PK-[protein] (overall reaction);
(1a) S-adenosyl-L-methionine + N-terminal-(A,S)PK-[protein] = S-adenosyl-L-homocysteine + N-terminal-N-methyl-N-(A,S)PK-[protein];
(1b) S-adenosyl-L-methionine + N-terminal-N-methyl-N-(A,S)PK-[protein] = S-adenosyl-L-homocysteine + N-terminal-N,N-dimethyl-N-(A,S)PK-[protein];
(1c) S-adenosyl-L-methionine + N-terminal-N,N-dimethyl-N-(A,S)PK-serine-[protein] = S-adenosyl-L-homocysteine + N-terminal-N,N,N-trimethyl-N-(A,S)PK-[protein];
(2) 2 S-adenosyl-L-methionine + N-terminal-PPK-[protein] = 2 S-adenosyl-L-homocysteine + N-terminal-N,N-dimethyl-N-PPK-[protein] (overall reaction);
(2a) S-adenosyl-L-methionine + N-terminal-PPK-[protein] = S-adenosyl-L-homocysteine + N-terminal-N-methyl-N-PPK-[protein];
(2b) S-adenosyl-L-methionine + N-terminal-N-methyl-N-PPK-[protein] = S-adenosyl-L-homocysteine + N-terminal-N,N-dimethyl-N-PPK-[protein]
Substrate
S-adenosyl-L-methionine [CPD:
C00019 ];
N-terminal-(A,S)PK-[protein];
N-terminal-N-methyl-N-(A,S)PK-[protein];
N-terminal-N,N-dimethyl-N-(A,S)PK-serine-[protein];
N-terminal-PPK-[protein];
N-terminal-N-methyl-N-PPK-[protein]
Product
S-adenosyl-L-homocysteine [CPD:
C00021 ];
N-terminal-N,N,N-trimethyl-N-(A,S)PK-[protein];
N-terminal-N-methyl-N-(A,S)PK-[protein];
N-terminal-N,N-dimethyl-N-(A,S)PK-[protein];
N-terminal-N,N-dimethyl-N-PPK-[protein];
N-terminal-N-methyl-N-PPK-[protein]
Comment
This enzyme methylates the N-terminus of target proteins containing the N-terminal motif [Ala/Pro/Ser]-Pro-Lys after the initiator L-methionine is cleaved. When the terminal amino acid is L-proline, the enzyme catalyses two successive methylations of its alpha-amino group. When the first amino acid is either L-alanine or L-serine, the enzyme catalyses three successive methylations.
The Pro-Lys in positions 2-3 cannot be exchanged for other amino acids [1,2].
History
EC 2.1.1.244 created 2012
Orthology
K16219 protein N-terminal methyltransferase
Genes
MYB : 102242208(NTMT1) 106724132
TOD : 119230945(NTMT1) 119250917
MGP : 100549024(NTMT1) 100549986
XLA : 734671(ntmt1.S) 735155(ntmt1.L)
XTR : 100145456(ntmt1) 116407067
SANH : 107691051 107693833
CCAR : 109079202 109080699
CAUA : 113049362 113075420
CGIB : 127957667 128006905
MASI : 127421721 127440177
PLEP : 121959866 121965519
SLAL : 111648140(ntmt1) 111671270
ARUT : 117421904 117964696
DSI : Dsimw501_GD10692(Dsim_GD10692)
BMOR : 101743128 101746959
BMAN : 114250958 114252517
MSEX : 115440975 115456136
DPL : KGM_206479 KGM_214199
BANY : 112047352 112058589
PMAC : 106707246 106710766
PPOT : 106099726 106108535
PRAP : 110998672 111003422
PNAP : 125048940 125056392
CCRC : 123696057 123706083
SLIU : 111352431 111365041
PPOI : 119095482 119095528
DPTE : 113794962 113799039
CEL : CELE_Y74C9A.3(homt-1)
BMY : BM_BM7124(Bma-homt-1)
MMER : 123557417 123557419
TAD : TRIADDRAFT_18419 TRIADDRAFT_49985
CSAT : 104724999 104760456 104771613
BNA : 106416578 106434267 106437298 111208530 111211944
RSZ : 108823976 108833511 108850469 108853298
GSJ : 114412620 114422926 114424762
LJA : Lj4g3v0333650.1(Lj4g3v0333650.1) Lj4g3v0333650.2(Lj4g3v0333650.2)
LANG : 109350827 109358046
CMAX : 111477809 111497490
CMOS : 111433034 111433513
CPEP : 111782110 111804120
QSU : 111991446 112020258 112025214 112035016
SSPL : 121769412 121772606
CSIN : 114265081 114293497
PSOM : 113280837 113289974
DOSA : Os03t0780900-01(Os03g0780900)
TAES : 123105256 123113514 123123045
PVIR : 120649558 120689879
SMO : SELMODRAFT_140975 SELMODRAFT_143000
NCS : NCAS_0B07330(NCAS0B07330)
NDI : NDAI_0B04650(NDAI0B04650)
TPF : TPHA_0O01360(TPHA0O01360)
TBL : TBLA_0F03410(TBLA0F03410)
TDL : TDEL_0D02090(TDEL0D02090)
KAF : KAFR_0C04340(KAFR0C04340)
KNG : KNAG_0C01870(KNAG0C01870)
CAL : CAALFM_C700490CA(CaO19.7069)
NTE : NEUTE1DRAFT76613(NEUTE1DRAFT_76613)
FOX : FOXG_10679 FOXG_10680
BFU : BCIN_03g02980(Bctae1)
AOR : AO090102000220 AO090103000204
ANG : ANI_1_940134(An15g06850)
AFV : AFLA_011669 AFLA_013318
ACHE : ACHE_20787A(TAE1_1) ACHE_30335A(TAE1_2)
ABP : AGABI1DRAFT35058(AGABI1DRAFT_35058)
ABV : AGABI2DRAFT148576(AGABI2DRAFT_148576)
SCM : SCHCO_02484794(SCHCODRAFT_02484794)
EHI : EHI_036850(283.t00004)
TET : TTHERM_000309909 TTHERM_00279880
PTM : GSPATT00005538001 GSPATT00030451001
SMIN : v1.2.005502.t1(symbB.v1.2.005502.t1) v1.2.010577.t1(symbB.v1.2.010577.t1)
SPAR : SPRG_13692 SPRG_17107
EHX : EMIHUDRAFT_100287 EMIHUDRAFT_124527
GTT : GUITHDRAFT_110071 GUITHDRAFT_114374
TBR : Tb927.6.2270 Tb927.6.2330
TBG : TbgDal_VI2050 TbgDal_VI2090
TVA : TVAG_2v0193630 TVAG_2v0224810 TVAG_2v0290400 TVAG_2v0406420 TVAG_2v0449160
» show all
Taxonomy
Reference
Authors
Webb KJ, Lipson RS, Al-Hadid Q, Whitelegge JP, Clarke SG
Title
Identification of protein N-terminal methyltransferases in yeast and humans.
Journal
Sequence
Reference
Authors
Tooley CE, Petkowski JJ, Muratore-Schroeder TL, Balsbaugh JL, Shabanowitz J, Sabat M, Minor W, Hunt DF, Macara IG
Title
NRMT is an alpha-N-methyltransferase that methylates RCC1 and retinoblastoma protein.
Journal
Sequence
Other DBs
ExPASy - ENZYME nomenclature database: 2.1.1.244