KEGG   ENZYME: 2.1.1.246
Entry
EC 2.1.1.246                Enzyme                                 
Name
[methyl-Co(III) methanol-specific corrinoid protein]---coenzyme M methyltransferase;
methyltransferase 2 (ambiguous);
mtaA (gene name)
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
Sysname
methylated methanol-specific corrinoid protein:CoM methyltransferase
Reaction(IUBMB)
a [methyl-Co(III) methanol-specific corrinoid protein] + CoM = methyl-CoM + a [Co(I) methanol-specific corrinoid protein] [RN:R10000]
Reaction(KEGG)
R10000;
(other) R09098
Substrate
[methyl-Co(III) methanol-specific corrinoid protein];
CoM [CPD:C03576]
Product
methyl-CoM [CPD:C03920];
[Co(I) methanol-specific corrinoid protein]
Comment
The enzyme, which is involved in methanogenesis from methanol, catalyses the transfer of a methyl group from a corrinoid protein (see EC 2.1.1.90, methanol---corrinoid protein Co-methyltransferase), where it is bound to the cobalt cofactor, to CoM, forming the substrate for EC 2.8.4.1, coenzyme-B sulfoethylthiotransferase, the enzyme that catalyses the final step in methanogenesis. Free methylcob(I)alamin can substitute for the corrinoid protein in vitro [5].
History
EC 2.1.1.246 created 2012
Pathway
ec00680  Methane metabolism
ec01120  Microbial metabolism in diverse environments
Orthology
K14080  [methyl-Co(III) methanol/glycine betaine-specific corrinoid protein]:coenzyme M methyltransferase
Genes
DEOCAY53_02410
DTIDesti_0877 Desti_2036 Desti_3217 Desti_5361
CALKHUE98_05430
DKUDesku_0050 Desku_0055 Desku_0060
FWADCMF_12445 DCMF_12460
TJRTherJR_0470 TherJR_0979 TherJR_0982
VGUHYG85_23900
ACAEHYG86_16410
AMEILV469_06415
SRISELR_27690(mtbA)
TAERGT409_09440
MSTMsp_0112(mtaA2) Msp_0182(mtaA4) Msp_0761(mtaA1) Msp_0774(mtaA3)
METBAW729_02405 AW729_06195 AW729_10490
MSIMsm_0518
MAROMarbSA_04020
METVK4897_03100
MELMetbo_1025
MEWMSWAN_1084
MCUBMCBB_1152(mtaA)
FPLFerp_1746 Ferp_1761
MBAMbar_A1054 Mbar_A1688 Mbar_A3639
MBYMSBRM_2354 MSBRM_2991 MSBRM_3313
MBWMSBRW_1093 MSBRW_3045 MSBRW_3668
MBARMSBR2_2320 MSBR2_2952 MSBR2_3267
MBAKMSBR3_1746 MSBR3_2381 MSBR3_3262
MACMA_0779(cmtM) MA_1615(cmtM) MA_2163(cmtM) MA_3613(cmtM) MA_4379(mtaA) MA_4559(cmtM)
MMAMM_0176(mtaA) MM_0505 MM_1070(mtaA) MM_1932(mtbA)
MMAZMmTuc01_0522 MmTuc01_1102 MmTuc01_2009
MMJMSMAS_0231 MSMAS_0801 MSMAS_1125 MSMAS_2752
MMACMSMAC_0237 MSMAC_0779 MSMAC_1671 MSMAC_2485
MVCMSVAZ_0217 MSVAZ_0373 MSVAZ_1364
MEKMSKOL_0211 MSKOL_0358 MSKOL_1307
MLSMSLAZ_0369 MSLAZ_1228 MSLAZ_1379 MSLAZ_2322 MSLAZ_2965
METMMSMTP_0087 MSMTP_0267 MSMTP_1139 MSMTP_1438 MSMTP_1905 MSMTP_1916 MSMTP_2416 MSMTP_2659
MEFMSWH1_0532 MSWH1_1021 MSWH1_1747 MSWH1_2472 MSWH1_2627
MEQMSWHS_0547 MSWHS_1092 MSWHS_1568 MSWHS_2411 MSWHS_2572
MSJMSSAC_0718 MSSAC_1597 MSSAC_1859 MSSAC_2001 MSSAC_3198 MSSAC_4064 MSSAC_4242
MSZMSSIH_0662 MSSIH_1027 MSSIH_1186 MSSIH_2365 MSSIH_3600 MSSIH_3745
MSWMSSIT_0690 MSSIT_1074 MSSIT_1253 MSSIT_2316 MSSIT_2424 MSSIT_3653 MSSIT_3824
MTHRMSTHT_0082 MSTHT_1209 MSTHT_1754
MTHEMSTHC_0648 MSTHC_1535 MSTHC_2085
MHORMSHOH_0293 MSHOH_0908 MSHOH_1347 MSHOH_1564 MSHOH_3370 MSHOH_3435
MFZAOB57_002025 AOB57_007965 AOB57_010270
MBUMbur_0452(mtaA) Mbur_0808 Mbur_1005(mtaA)
MMETMCMEM_0940 MCMEM_1137 MCMEM_2051
MELOJ7W08_02985 J7W08_04245
MMHMmah_0008 Mmah_0518 Mmah_0901 Mmah_0970 Mmah_1478
MHAZBHR79_00830 BHR79_03425 BHR79_08720 BHR79_09050
MEVMetev_0012 Metev_0013 Metev_0942
MZHMzhil_0011 Mzhil_0619 Mzhil_1798 Mzhil_1957
MPYMpsy_0341 Mpsy_0911
MZIHWN40_04040 HWN40_06230 HWN40_07880
MHZMetho_0761 Metho_0776 Metho_1260
MEAMMU439_00860 MU439_01115 MU439_01140(mtaA) MU439_02360 MU439_05065(mtaA)
TARTALC_00742
MAXMMALV_03890 MMALV_05210 MMALV_09620
MERMMINT_07210 MMINT_13560 MMINT_15760
MEARMpt1_c01960(mtAA1)
MEUZKRP56_02065 KRP56_04335
MARCAR505_1035 AR505_1063 AR505_1404 AR505_1575
 » show all
Reference
1  [PMID:8702528]
  Authors
LeClerc GM, Grahame DA
  Title
Methylcobamide:coenzyme M methyltransferase isozymes from Methanosarcina barkeri. Physicochemical characterization, cloning, sequence analysis, and heterologous gene expression.
  Journal
J Biol Chem 271:18725-31 (1996)
DOI:10.1074/jbc.271.31.18725
  Sequence
Reference
2  [PMID:8654414]
  Authors
Harms U, Thauer RK
  Title
Methylcobalamin: coenzyme M methyltransferase isoenzymes MtaA and MtbA from Methanosarcina barkeri. Cloning, sequencing and differential transcription of the encoding genes, and functional overexpression of the mtaA gene in Escherichia coli.
  Journal
Eur J Biochem 235:653-9 (1996)
DOI:10.1111/j.1432-1033.1996.00653.x
  Sequence
Reference
3  [PMID:9363780]
  Authors
Sauer K, Thauer RK
  Title
Methanol:coenzyme M methyltransferase from Methanosarcina barkeri. Zinc dependence and thermodynamics of the methanol:cob(I)alamin methyltransferase reaction.
  Journal
Eur J Biochem 249:280-5 (1997)
DOI:10.1111/j.1432-1033.1997.t01-1-00280.x
Reference
4  [PMID:9057830]
  Authors
Sauer K, Harms U, Thauer RK
  Title
Methanol:coenzyme M methyltransferase from Methanosarcina barkeri. Purification, properties and encoding genes of the corrinoid protein MT1.
  Journal
Eur J Biochem 243:670-7 (1997)
DOI:10.1111/j.1432-1033.1997.t01-1-00670.x
Reference
5  [PMID:10215883]
  Authors
Sauer K, Thauer RK
  Title
Methanol:coenzyme M methyltransferase from Methanosarcina barkeri -- substitution of the corrinoid harbouring subunit MtaC by free cob(I)alamin.
  Journal
Eur J Biochem 261:674-81 (1999)
DOI:10.1046/j.1432-1327.1999.00355.x
Other DBs
ExplorEnz - The Enzyme Database: 2.1.1.246
IUBMB Enzyme Nomenclature: 2.1.1.246
ExPASy - ENZYME nomenclature database: 2.1.1.246
BRENDA, the Enzyme Database: 2.1.1.246

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