KEGG ENZYME: 2.1.1.248

ENZYME: 2.1.1.248

Entry

EC 2.1.1.248                Enzyme

Name

methylamine---corrinoid protein Co-methyltransferase;
mtmB (gene name);
monomethylamine methyltransferase

Class

Transferases;
Transferring one-carbon groups;
Methyltransferases

Sysname

monomethylamine:5-hydroxybenzimidazolylcobamide Co-methyltransferase

Reaction(IUBMB)

methylamine + a [Co(I) methylamine-specific corrinoid protein] = a [methyl-Co(III) methylamine-specific corrinoid protein] + NH3 [RN:R10014]

Reaction(KEGG)

R10014;
(other) R09998

Substrate

methylamine [CPD:C00218];
[Co(I) methylamine-specific corrinoid protein]

Product

[methyl-Co(III) methylamine-specific corrinoid protein];
NH3 [CPD:C00014]

Comment

The enzyme, which catalyses the transfer of a methyl group from methylamine to a methylamine-specific corrinoid protein (MtmC), is involved in methanogenesis from methylamine. The enzyme contains the unusual amino acid pyrrolysine [3]. Methylation of the corrinoid protein requires the central cobalt to be in the Co(I) state. During methylation the cobalt is oxidized to the Co(III) state. The methylated corrinoid protein is substrate for EC 2.1.1.247, methylated methylamine-specific corrinoid protein:coenzyme M methyltransferase.

History

EC 2.1.1.248 created 2012

Pathway

ec00680

Methane metabolism

ec01120

Microbial metabolism in diverse environments

Orthology

K16176

methylamine---corrinoid protein Co-methyltransferase

Genes

SDF:	ACG33_12615

DOE:	DENOEST_1155 DENOEST_1170

DEO:	CAY53_02475

DML:	Dmul_02380(mtmB)

DEK:	DSLASN_08540

CTHD:

CDO33_18585

DAE:

Dtox_0025

FWA:	DCMF_02210 DCMF_07385 DCMF_08180 DCMF_08800 DCMF_08825 DCMF_12475 DCMF_12480 DCMF_14680 DCMF_24475

ELIM:

B2M23_05155 B2M23_05160

VGU:	HYG85_16645

VPY:	HZI73_14240

AAR:	Acear_1719

SRI:	SELR_27700(mtmB)

MANA:

MAMMFC1_01966 MAMMFC1_01967

SSPH:

SPSPH_029870

SOVA:

SOV_01610

SSID:

SPSIL_001440 SPSIL_001610

ETT:	AZF37_07305

PSUB:

Pelsub_P2997

OCK:	EXM22_00710

CPOR:

BED41_14915

MBA:	Mbar_A0843 Mbar_A0846

MBY:	MSBRM_0150 MSBRM_1850

MBW:	MSBRW_0156 MSBRW_0158 MSBRW_1099

MBAR:

MSBR2_0560 MSBR2_2231

MBAK:

MSBR3_0508 MSBR3_2282 MSBR3_2284

MAC:	MA_0144(mtmB1) MA_2972(mtmB2)

MMA:	MM_1436(mtmB) MM_1437(mtmB) MM_3335(mtmB) MM_3336(mtmB)

MMAZ:

MmTuc01_3441

MMJ:	MSMAS_1346 MSMAS_3227

MMAC:

MSMAC_2710 MSMAC_3249

MVC:	MSVAZ_1890 MSVAZ_3365

MEK:	MSKOL_1876 MSKOL_3326

MLS:	MSLAZ_3202 MSLAZ_3204

METM:

MSMTP_1524 MSMTP_3128

MEF:	MSWH1_1133 MSWH1_3075 MSWH1_3077

MEQ:	MSWHS_1207 MSWHS_3286

MSJ:	MSSAC_0121 MSSAC_0126 MSSAC_1323

MSZ:	MSSIH_0112 MSSIH_1617

MSW:	MSSIT_0113 MSSIT_0116 MSSIT_1628

MTHR:

MSTHT_1926

MTHE:

MSTHC_1357

MHOR:

MSHOH_1819 MSHOH_3974

MMET:

MCMEM_0625 MCMEM_0969 MCMEM_0978

MELO:

J7W08_01185 J7W08_01690

MMH:

Mmah_1399

MHAZ:

BHR79_06675 BHR79_07765 BHR79_07770 BHR79_07860 BHR79_07865

MPOT:

BKM01_02925 BKM01_04010 BKM01_08585

MEV:	Metev_1068 Metev_1350 Metev_1467

MZH:	Mzhil_0326 Mzhil_0630 Mzhil_1522 Mzhil_1525

MPY:	Mpsy_1674 Mpsy_1675(mtmB1) Mpsy_1677(mtmB2) Mpsy_1711(mtmB3)

MZI:	HWN40_00850

MMAV:

RE476_09505

MSEB:

RE474_06460

MHZ:	Metho_0013 Metho_0035 Metho_0036

MEHF:

MmiHf6_09310

MEES:

MmiEs2_11900

TAR:	TALC_01093 TALC_01094

MAX:	MMALV_11290 MMALV_11300

MER:	MMINT_08870 MMINT_08950

MEAR:

Mpt1_c05730(mtmB11) Mpt1_c05760(mtmB13)

MARC:

AR505_1327 AR505_1328

TPE:

Tpen_1211

THB:	N186_07205

THF:	MA03_03050

THEL:

IG193_00145

» show all

Reference

1 [PMID:9195968]

Authors

Burke SA, Krzycki JA

Title

Reconstitution of Monomethylamine:Coenzyme M methyl transfer with a corrinoid protein and two methyltransferases purified from Methanosarcina barkeri.

Journal

J Biol Chem 272:16570-7 (1997)
DOI:10.1074/jbc.272.26.16570

Sequence

[mba:Mbar_A0843]

Reference

2 [PMID:9642198]

Authors

Burke SA, Lo SL, Krzycki JA

Title

Clustered genes encoding the methyltransferases of methanogenesis from monomethylamine.

Journal

J Bacteriol 180:3432-40 (1998)
DOI:10.1128/JB.180.13.3432-3440.1998