KEGG   ENZYME: 2.1.1.249Help
Entry
EC 2.1.1.249                Enzyme                                 

Name
dimethylamine---corrinoid protein Co-methyltransferase;
mtbB (gene name);
dimethylamine methyltransferase
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
BRITE hierarchy
Sysname
dimethylamine:5-hydroxybenzimidazolylcobamide Co-methyltransferase
Reaction(IUBMB)
dimethylamine + a [Co(I) dimethylamine-specific corrinoid protein] = a [methyl-Co(III) dimethylamine-specific corrinoid protein] + methylamine [RN:R10015]
Reaction(KEGG)
R10015;
(other) R09999
Show
Substrate
dimethylamine [CPD:C00543];
[Co(I) dimethylamine-specific corrinoid protein]
Product
[methyl-Co(III) dimethylamine-specific corrinoid protein];
methylamine [CPD:C00218]
Comment
The enzyme, which catalyses the transfer of a methyl group from dimethylamine to a dimethylamine-specific corrinoid protein (MtbC), is involved in methanogenesis from dimethylamine. The enzyme contains the unusual amino acid pyrrolysine [3]. Methylation of the corrinoid protein requires the central cobalt to be in the Co(I) state. During methylation the cobalt is oxidized to the Co(III) state. The methylated corrinoid protein is substrate for EC 2.1.1.247, methylated methylamine-specific corrinoid protein:coenzyme M methyltransferase.
History
EC 2.1.1.249 created 2012
Pathway
ec00680  Methane metabolism
ec01120  Microbial metabolism in diverse environments
Orthology
K16178  dimethylamine---corrinoid protein Co-methyltransferase
Genes
TPZ: Tph_c27710(mtbB1) Tph_c27720(mtbB2)
AAR: Acear_1273
MAC: MA_0532(mtbB) MA_0933(mtbB) MA_2425(mtbB)
MBY: MSBRM_0457 MSBRM_0926 MSBRM_0957
MBW: MSBRW_1129 MSBRW_2236 MSBRW_3223
MBAR: MSBR2_1469 MSBR2_1924
MBAK: MSBR3_0479 MSBR3_0618 MSBR3_1955
MMA: MM_1693(mtbB) MM_2051(mtbB) MM_2962(mtbB)
MMAZ: MmTuc01_1772
MMJ: MSMAS_1746 MSMAS_2635 MSMAS_2980
MPY: Mpsy_1680(mtbB) Mpsy_1701(mtbB2) Mpsy_1702(mtbB3)
MARC: AR505_1332
 » show all
Taxonomy
Reference
1  [PMID:9654067]
  Authors
Wassenaar RW, Keltjens JT, van der Drift C, Vogels GD
  Title
Purification and characterization of dimethylamine:5-hydroxybenzimidazolyl-cobamide methyltransferase from Methanosarcina barkeri Fusaro.
  Journal
Eur J Biochem 253:692-7 (1998)
DOI:10.1046/j.1432-1327.1998.2530692.x
Reference
2  [PMID:10852929]
  Authors
Ferguson DJ Jr, Gorlatova N, Grahame DA, Krzycki JA
  Title
Reconstitution of dimethylamine:coenzyme M methyl transfer with a discrete corrinoid protein and two methyltransferases purified from Methanosarcina barkeri.
  Journal
J Biol Chem 275:29053-60 (2000)
DOI:10.1074/jbc.M910218199
  Sequence
Reference
3  [PMID:15450490]
  Authors
Krzycki JA
  Title
Function of genetically encoded pyrrolysine in corrinoid-dependent methylamine methyltransferases.
  Journal
Curr Opin Chem Biol 8:484-91 (2004)
DOI:10.1016/j.cbpa.2004.08.012
Other DBs
ExplorEnz - The Enzyme Database: 2.1.1.249
IUBMB Enzyme Nomenclature: 2.1.1.249
ExPASy - ENZYME nomenclature database: 2.1.1.249
BRENDA, the Enzyme Database: 2.1.1.249

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