KEGG   ENZYME: 2.1.1.257
Entry
EC 2.1.1.257                Enzyme                                 
Name
tRNA (pseudouridine54-N1)-methyltransferase;
TrmY;
m1Psi methyltransferase
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
Sysname
S-adenosyl-L-methionine:tRNA (pseudouridine54-N1)-methyltransferase
Reaction(IUBMB)
S-adenosyl-L-methionine + pseudouridine54 in tRNA = S-adenosyl-L-homocysteine + N1-methylpseudouridine54 in tRNA
Substrate
S-adenosyl-L-methionine [CPD:C00019];
pseudouridine54 in tRNA
Product
S-adenosyl-L-homocysteine [CPD:C00021];
N1-methylpseudouridine54 in tRNA
Comment
While this archaeal enzyme is specific for the 54 position and does not methylate pseudouridine at position 55, the presence of pseudouridine at position 55 is necessary for the efficient methylation of pseudouridine at position 54 [2,3].
History
EC 2.1.1.257 created 2012
Orthology
K16317  tRNA (pseudouridine54-N1)-methyltransferase
Genes
LYJFKV23_09695(trmY)
LLZLYB30171_01121
LUMCNR27_09035
THESFHQ07_02155(trmY)
THEHG7079_08865(trmY)
TCNH9L16_14110(trmY)
XBCELE36_14165(trmY)
DTXATSB10_14670
XBAC7S18_10395
VCHVC_A1059
VCFIR04_04680
VCSMS6_A1089
VCEVch1786_II0744
VCQEN18_03535
VCJVCD_000281
VCIO3Y_18448
VCOVC0395_0182
VCRVC395_A1082
VCMVCM66_A1017
VCLVCLMA_B0808
VCXVAA049_1942
VCZVAB027_1071
VVUVV2_1434
VVYVVA0268
VVMVVMO6_03226
VVLVV93_v1c32550
VPAVPA0140
VPBVPBB_A0130
VPKM636_08175
VPFM634_18755
VPHVPUCM_20134
VHAVIBHAR_06835
VCAM892_18410
VAGN646_3572
VEXVEA_000860
VDBAL552_02605
VHRAL538_20320
VNAPN96_16720
VOWA9237_16700
VROBSZ04_05050
VSPVS_II1248
VEJVEJY3_16226
VANVAA_00947
LAGN175_15920
VAUVANGNB10_cII0813c
VTUIX91_23425
VEUIXK98_04255(trmY)
VMIAL543_01025
VSCVSVS12_04457(trmY)
VSHBSZ05_13140
VQICCZ37_14580
VTAB1432
VAFD1115_16105(trmY)
VNLD3H41_21980(trmY)
VCCFAZ90_16720(trmY)
VASGT360_14850(trmY)
VKABTD91_16085(trmY)
VZIG5S32_18505(trmY)
VNVIF132_05720(trmY)
VGIMID13_21990(trmY)
VSLLTQ54_18395(trmY)
VPLSA104470976_02915
VPGLZI70_14720(trmY)
VTICEQ48_03180
VCRAIS519_24030(trmY)
VLEISX51_21355(trmY)
VSYK08M4_41330
VMTQYQ96_17515(trmY)
PPRPBPRA1526(VVA0268)
PGBH744_1c0712
PDSCAY62_17570
SFRSfri_1778
SBLSbal_2539
SBMShew185_2532
SBNSbal195_2654
SBPSbal223_1812
SBTSbal678_2657
SBSSbal117_2676
SBBSbal175_1839
SHEWCKQ84_21555
SLJEGC82_12800(trmY)
SPOLFH971_08750(trmY)
SGLAFJ709_10745(trmY)
SINVK8B83_14205(trmY)
SVMKDH10_000758(trmY)
SOGRA178_09830(trmY)
ALGAQULUS_02540
LGTE4T54_05445
ABOABO_1734
ALNAS19_17900
ADIB5T_04327
AXEP40_21080
APACS7S_12770
PAUUE8A73_046525
SAMYDB32_004399
LLUAKJ09_07380
ADAA5CPEGH6_03280
BANABARAN1_0567(trmY)
BIHBIP78_0510
MJAMJ_1640
MFEMefer_1329
MVUMetvu_0917
MFSMFS40622_0636
MIFMetin_0292
MJHJH146_0540
MESGMLAUSG7_0015(trmY)
MESAMLASG1_1021(trmY)
MIGMetig_1562
MMPMMP0924
MMQMmarC5_0731
MMXMmarC6_1731
MMZMmarC7_0171
MMDGYY_05340
MMAKMMKA1_10220
MMAOMMOS7_10000
MMADMMJJ_00670
MAEMaeo_0669
MVNMevan_0217
MVOMvol_0946
MOKMetok_1422
METFCFE53_06135
MTEEMTTB_03440
METZMETMT2_1093
MFVMfer_0333
MKAMK1018
AFUAF_1056
AFGAFULGI_00011530
APOArcpr_1449
AVEArcve_0379
ASTAsulf_01984
FPLFerp_0695
GACGACE_1384
GAHGAH_01072
PFUPF1403
PFIPFC_06240
PHOPH1401(PH1401)
PABPAB1866
PYNPNA2_1979
PYAPYCH_07270
PYSPy04_1348
PYCTQ32_09660
TKOTK0554
TONTON_0782
TGATGAM_1665
TBATERMP_00658
THATAM4_1784
THMCL1_0088
TLTOCC_08450
THSTES1_0609
TNUBD01_0967
TEUTEU_10635
TGYX802_01245
THVADU37_CDS08580
TCHCHITON_1673
TPEPA0127_01935
TPIEA7C91_10105
TGGA3K92_02155
TBSA3L01_04710
THHCDI07_04580
TSLA3L11_08850
TTDA3L14_05790
TPRFA3L09_04375
TRLA3L10_01915
TPAFA3L08_07260
MBAMbar_A3229
MBYMSBRM_1397
MBWMSBRW_1502
MBARMSBR2_1010
MBAKMSBR3_0431
MACMA_1448
MMAMM_2534
MMAZMmTuc01_2588
MMJMSMAS_2170
MMACMSMAC_2283
MVCMSVAZ_2175
MEKMSKOL_2168
MLSMSLAZ_0889
METMMSMTP_2029
MEFMSWH1_1370
MEQMSWHS_3208
MSJMSSAC_1496
MSZMSSIH_1463
MSWMSSIT_1519
MTHRMSTHT_0618
MTHEMSTHC_0102
MHORMSHOH_2777
MFZAOB57_013855(trmY)
MBUMbur_0907
MMETMCMEM_1261
MELOJ7W08_04925(trmY)
MMHMmah_1033
MHAZBHR79_08405
MEVMetev_1628
MZHMzhil_1274
MPYMpsy_2777
MZIHWN40_08765(trmY)
MMAVRE476_01400(trmY)
MSEBRE474_11090(trmY)
MHZMetho_0715
MEHFMmiHf6_00670
MEESMmiEs2_09350
MTPMthe_0609
MCJMCON_2748
MHIMhar_0820
MHUMhun_3024
MRTJKHC33_01775(trmY)
MLAMlab_1566
MEMMemar_1963
MBGBN140_0303
MEMAMMAB1_0381(trmY)
MCHKMchiMG62_22750
MSUMOH143_04070(trmY)
MRCR6Y96_00155(trmY)
MPIMpet_1855
MENDL6E24_01120(trmY)
MEFWF1737_03140(trmY)
MAQERJ40_04110(trmY)
MOUOU421_09790(trmY)
MESBL1S32_00995(trmY)
MANQL1994_03990(trmY)
MBNMboo_1876
MFOMetfor_1110
MPLMpal_0545
MPDMCP_2651
MEZMtc_1405
RCIRCIX2338
HALVNG_1980C
HSLOE_3775R(trmY)
HANRLJ422_07965(trmY)
HDLHALDL1_14815
HHBHhub_3285(trmY)
HAGSJT689_09305(trmY)
HABOJRZ79_07700(trmY)
HNOLT974_13220(trmY)
HLULT972_01560(trmY)
HALHHTSR_0146
HHSRHSR6_0147(trmY)
HSUHLASF_0188
HSFHLASA_0188
HAHSHSRCO_0261(rsmE)
SALRFQU85_06215(trmY)
HALREFA46_002285(trmY)
HJEHacjB3_04640
HDOMUK72_01505(trmY)
HALXM0R89_03840(trmY)
HAXZM0R88_04015(trmY)
HAYCNGM10_11555(trmY)
HAXYNGM07_08825(trmY)
SALIL593_14300
SAIMK0C01_07580(trmY)
HARAAArcS_2781(rsmE)
HMArrnAC3253
HHIHAH_0499
HHNHISP_02580
HABSG26_10375
HTABVU17_02455
HALJG9465_17775(trmY)
HSINKDQ40_11040(trmY)
NPHNP_2324A(trmY)
NMONmlp_3002(trmY)
NHOHWV23_14145(trmY)
NSALHWV07_17660(trmY)
HUTHuta_1107
HTIHTIA_0957
HALAHrd1104_08840(trmY)
HASVSVXHr_0513(trmY)
HABNHBNXHr_0491(trmY)
HMUHmuk_1447
HALZE5139_10395(trmY)
HALLLC1Hm_0919(trmY)
HAZPGBQ70_10390(trmY)
HALIBV210_11850
HSNDV733_01840
HDSHSR122_2260(rsmE)
HRRHZS55_07110(trmY)
HPELHZS54_07690(trmY)
HLTI7X12_07065(trmY)
HARCHARCEL1_00235
SSAIN0B31_11505(trmY)
HAADMW046_06540(trmY)
HVOHVO_1989(trmY)
HMEHFX_2082
HGIABY42_09705
HALEG3A49_12935(trmY)
HLSKU306_02555(trmY)
HLNSVXHx_2630(trmY)
HBOHbor_05920
HAQDU484_04645
HAJDU500_05060
HAERDU502_02970(trmY)
HRAEI982_10640(trmY)
HLMDV707_02135
HALMFCF25_02245(trmY)
HLAHlac_1588
HALPDOS48_07950
HALBEKH57_01395(trmY)
HEZZEO776_09630(trmY)
HALQHrr1229_013340(trmY)
HSSJ7656_06435(trmY)
HSAIHPS36_11960(trmY)
HAZZKI388_08080(trmY)
SRUBC2R22_18955
HAEhalTADL_0770
HALNB4589_012630(trmY)
HALGHUG10_10185(trmY)
HALUHUG12_06060(trmY)
HMPK6T50_07405(trmY)
HREK6T36_07215(trmY)
HRMK6T25_08595(trmY)
HACBHbl1158_13795(trmY)
HDFAArcSl_1898(trmY)
HAAAAArcCO_0245(rsmE)
HAHHalar_2006
HTUHtur_0735
HSALJMJ58_05270(trmY)
HAKZJ0X25_10875(trmY)
NMGNmag_2697(trmY)
HXAHalxa_3611
NATNJ7G_4014
NPENatpe_3189
NVRFEJ81_15310(trmY)
NPLFGF80_13630(trmY)
NAYHYG81_13600(trmY)
HALYHYG82_16400(trmY)
HJTDVR14_06085
HLOJ0X27_07415(trmY)
NGENatgr_1028
HRUHalru_2514
SAWLNGM29_01650(trmY)
HALVNGM15_11250(trmY)
NOUNatoc_0809
HLRHALLA_08100
HLCCHINAEXTREME00910
NAJB1756_04840
NAGAArcMg_0666
NANAArc1_2925
NARAQQ977_14145(trmY)
NBGDV706_03625
NASGCU68_09855(trmY)
HDABB347_00740
MELUMTLP_08310
MEAMMU439_04010
MEAEQEN48_06175
TARTALC_00917
MAXMMALV_07840
MERMMINT_10980
MEARMpt1_c04950(trmY)
MEUZKRP56_04230(trmY)
MARCAR505_0759
ABIAboo_0918
ACFAciM339_0084
APEAPE_0797
ACJACAM_0562
IHOIgni_0291 Igni_1054
IISEYM_07865 EYM_07890
HBUHbut_0777
PFMPyrfu_0165 Pyrfu_0273
PDLPyrde_0531 Pyrde_1283
PAREPYJP_05150
PAIPAE2175
PISPisl_0598 Pisl_0819
PCLPcal_1090 Pcal_1725
PASPars_0801 Pars_1770
PYRP186_0414 P186_1261
POGPogu_0362 Pogu_1531
TNETneu_1364 Tneu_1518
PYWPYWP30_01444
TTNTTX_0164a TTX_0616
TUZTUZN_0254 TUZN_1182
TPETpen_0460 Tpen_0549
THBN186_03260 N186_04240
TCBTCARB_1720 TCARB_1875
THFMA03_06830 MA03_07330
THELIG193_04855 IG193_05250
LOKILokiarch_47640(trmY_1) Lokiarch_49910(trmY_2)
ARGQT11_C0001G0583 QT11_C0001G0621
 » show all
Reference
1  [PMID:21098051]
  Authors
Chen HY, Yuan YA
  Title
Crystal structure of Mj1640/DUF358 protein reveals a putative SPOUT-class RNA methyltransferase.
  Journal
J Mol Cell Biol 2:366-74 (2010)
DOI:10.1093/jmcb/mjq034
  Sequence
[mja:MJ_1640]
Reference
2  [PMID:22274954]
  Authors
Wurm JP, Griese M, Bahr U, Held M, Heckel A, Karas M, Soppa J, Wohnert J
  Title
Identification of the enzyme responsible for N1-methylation of pseudouridine 54 in archaeal tRNAs.
  Journal
RNA 18:412-20 (2012)
DOI:10.1261/rna.028498.111
  Sequence
[hvo:HVO_1989] [mja:MJ_1640]
Reference
3  [PMID:22274953]
  Authors
Chatterjee K, Blaby IK, Thiaville PC, Majumder M, Grosjean H, Yuan YA, Gupta R, de Crecy-Lagard V
  Title
The archaeal COG1901/DUF358 SPOUT-methyltransferase members, together with pseudouridine synthase Pus10, catalyze the formation of 1-methylpseudouridine at  position 54 of tRNA.
  Journal
RNA 18:421-33 (2012)
DOI:10.1261/rna.030841.111
  Sequence
[hvo:HVO_1989] [mja:MJ_1640]
Other DBs
ExplorEnz - The Enzyme Database: 2.1.1.257
IUBMB Enzyme Nomenclature: 2.1.1.257
ExPASy - ENZYME nomenclature database: 2.1.1.257
BRENDA, the Enzyme Database: 2.1.1.257

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