KEGG   ENZYME: 2.1.1.272
Entry
EC 2.1.1.272                Enzyme                                 

Name
cobalt-factor III methyltransferase;
CbiH60 (gene name);
S-adenosyl-L-methionine:cobalt-factor III 17-methyltransferase (ring contracting)
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
Sysname
S-adenosyl-L-methionine:cobalt-factor III C17-methyltransferase (ring contracting)
Reaction(IUBMB)
S-adenosyl-L-methionine + cobalt-factor III + reduced acceptor = S-adenosyl-L-homocysteine + cobalt-precorrin-4 + acceptor [RN:R11580]
Reaction(KEGG)
R11580
Substrate
S-adenosyl-L-methionine [CPD:C00019];
cobalt-factor III [CPD:C17401];
reduced acceptor [CPD:C00030]
Product
S-adenosyl-L-homocysteine [CPD:C00021];
cobalt-precorrin-4;
acceptor [CPD:C00028]
Comment
Isolated from the bacterium Bacillus megaterium. The enzyme, which participates in the anaerobic (early cobalt insertion) pathway of adenosylcobalamin biosynthesis, catalyses a crucial reaction where the tetrapyrrole ring contracts as a result of methylation of C-17. Contains a [4Fe-4S] cluster. It can also convert cobalt-precorrin-3 to cobalt-precorrin-4. The reductant may be thioredoxin. See EC 2.1.1.131, precorrin-3B C17-methyltransferase, for the corresponding enzyme that participates in the aerobic cobalamin biosynthesis pathway.
History
EC 2.1.1.272 created 2013
Pathway
ec00860  Porphyrin and chlorophyll metabolism
ec01100  Metabolic pathways
Orthology
K21479  cobalt-factor III methyltransferase
Genes
VTA: B1454(cbiH)
CVC: BKX93_20970
CHRO: CXB49_14705
CHRI: DK842_00190
CRZ: D1345_10920(cobJ)
CHRB: DK843_05475
CHRM: FYK34_02945(cobJ)
AMAH: DLM_2224
AQS: DKK66_15365
RSL: RPSI07_mp0502
RSN: RSPO_m00966(cbiJH)
HPSE: HPF_17610(cbiH)
HRB: Hrubri_2738(cbiJH)
MPLI: E1742_01370(cobJ)
DAT: HRM2_02260(cobJ)
BMQ: BMQ_2619(cbiH)
BMD: BMD_2606(cbiH)
BMEG: BG04_5075(cobJ)
BACO: OXB_1232
BEO: BEH_20915
BKW: BkAM31D_09040(cbiH)
BACQ: DOE78_12790(cobJ)
GKA: GK1805
GTN: GTNG_1694(cbiH)
GGH: GHH_c18480(cbiH)
GEA: GARCT_01823(cbiH)
PTB: DER53_12350(cobJ)
AFL: Aflv_2180
ANM: GFC28_882(cobJ)
AAMY: GFC30_2119(cobJ)
ANL: GFC29_744(cobJ)
AND: GRQ40_08245(cobJ)
LSP: Bsph_3446
LYS: LBYS11_13880(cobJ)
LYB: C3943_14025(cobJ)
LPAK: GDS87_14760(cobJ)
BBE: BBR47_12450(cbiH60)
BAGR: BA6348_22645(cobJ)
BRW: GOP56_21810(cobJ)
PPY: PPE_04548
PPM: PPSC2_23695(cbiH)
PPO: PPM_4709(cbiH)
PPOL: X809_40250
PPQ: PPSQR21_047960(cobJ)
PPOY: RE92_13565
PMW: B2K_32630
PSAB: PSAB_08885
PRI: PRIO_2096
PCHI: PC41400_25735(cobJ)
ASOC: CB4_00364(cbiH)
BTS: Btus_0413
KYR: CVV65_03595(cobJ)
TAB: CIG75_16950(cobJ)
SIV: SSIL_2492
SOB: CSE16_00750(cobJ)
KUR: ASO14_1788(cobJ)
LFB: C1X05_07750(cobJ)
SAY: TPY_1366(cbiGH-cobJ)
DGO: DGo_CA0808(cbiJ)
LKM: EFP84_20220(cobJ)
PCAG: NCTC12856_00426(cbiH)
PSAC: PSM36_1722
DYS: G7050_11320(cobJ)
 » show all
Reference
1  [PMID:23155054]
  Authors
Moore SJ, Biedendieck R, Lawrence AD, Deery E, Howard MJ, Rigby SE, Warren MJ
  Title
Characterization of the enzyme CbiH60 involved in anaerobic ring contraction of the cobalamin (vitamin B12) biosynthetic pathway.
  Journal
J Biol Chem 288:297-305 (2013)
DOI:10.1074/jbc.M112.422535
Other DBs
ExplorEnz - The Enzyme Database: 2.1.1.272
IUBMB Enzyme Nomenclature: 2.1.1.272
ExPASy - ENZYME nomenclature database: 2.1.1.272
BRENDA, the Enzyme Database: 2.1.1.272

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