KEGG   ENZYME: 2.1.1.292Help
Entry
EC 2.1.1.292                Enzyme                                 

Name
carminomycin 4-O-methyltransferase;
DnrK;
DauK
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
BRITE hierarchy
Sysname
S-adenosyl-L-methionine:carminomycin 4-O-methyltransferase
Reaction(IUBMB)
S-adenosyl-L-methionine + carminomycin = S-adenosyl-L-homocysteine + daunorubicin [RN:R06693]
Reaction(KEGG)
R06693;
(other) R06687
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Substrate
S-adenosyl-L-methionine [CPD:C00019];
carminomycin [CPD:C12432]
Product
S-adenosyl-L-homocysteine [CPD:C00021];
daunorubicin [CPD:C01907]
Comment
The enzymes from the Gram-positive bacteria Streptomyces sp. C5 and Streptomyces peucetius are involved in the biosynthesis of the anthracycline daunorubicin. In vitro the enzyme from Streptomyces sp. C5 also catalyses the 4-O-methylation of 13-dihydrocarminomycin, rhodomycin D and 10-carboxy-13-deoxycarminomycin [3].
History
EC 2.1.1.292 created 2013
Pathway
ec01057  Biosynthesis of type II polyketide products
ec01130  Biosynthesis of antibiotics
Orthology
K15954  carminomycin 4-O-methyltransferase
Reference
1  [PMID:8360627]
  Authors
Connors NC, Strohl WR
  Title
Partial purification and properties of carminomycin 4-O-methyltransferase from Streptomyces sp. strain C5.
  Journal
J Gen Microbiol 139 Pt 6:1353-62 (1993)
DOI:10.1099/00221287-139-6-1353
  Sequence
Reference
2  [PMID:15273252]
  Authors
Jansson A, Koskiniemi H, Mantsala P, Niemi J, Schneider G
  Title
Crystal structure of a ternary complex of DnrK, a methyltransferase in daunorubicin biosynthesis, with bound products.
  Journal
J Biol Chem 279:41149-56 (2004)
DOI:10.1074/jbc.M407081200
  Sequence
Reference
3  [PMID:9098063]
  Authors
Dickens ML, Priestley ND, Strohl WR.
  Title
In vivo and in vitro bioconversion of epsilon-rhodomycinone glycoside to doxorubicin: functions of DauP, DauK, and DoxA.
  Journal
J Bacteriol 179:2641-50 (1997)
DOI:10.1128/JB.179.8.2641-2650.1997
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 2.1.1.292
IUBMB Enzyme Nomenclature: 2.1.1.292
ExPASy - ENZYME nomenclature database: 2.1.1.292
BRENDA, the Enzyme Database: 2.1.1.292

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