KEGG   ENZYME: 2.1.1.324
Entry
EC 2.1.1.324                Enzyme                                 

Name
dTDP-4-amino-2,3,4,6-tetradeoxy-D-glucose N,N-dimethyltransferase;
SpnS;
TDP-4-amino-2,3,6-trideoxy-D-glucose N,N-dimethyltransferase
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
Sysname
S-adenosyl-L-methionine:dTDP-4-amino-2,3,4,6-tetradeoxy-alpha-D-erythro-hexopyranose N,N-dimethyltransferase
Reaction(IUBMB)
2 S-adenosyl-L-methionine + dTDP-4-amino-2,3,4,6-tetradeoxy-alpha-D-erythro-hexopyranose = 2 S-adenosyl-L-homocysteine + dTDP-alpha-D-forosamine (overall reaction) [RN:R08932];
(1a) S-adenosyl-L-methionine + dTDP-4-amino-2,3,4,6-tetradeoxy-alpha-D-erythro-hexopyranose = S-adenosyl-L-homocysteine + dTDP-4-(methylamino)-2,3,4,6-tetradeoxy-alpha-D-erythro-hexopyranose [RN:R11452];
(1b) S-adenosyl-L-methionine + dTDP-4-(methylamino)-2,3,4,6-tetradeoxy-alpha-D-erythro-hexopyranose = S-adenosyl-L-homocysteine + dTDP-alpha-D-forosamine [RN:R11453]
Reaction(KEGG)
Substrate
S-adenosyl-L-methionine [CPD:C00019];
dTDP-4-amino-2,3,4,6-tetradeoxy-alpha-D-erythro-hexopyranose [CPD:C18033];
dTDP-4-(methylamino)-2,3,4,6-tetradeoxy-alpha-D-erythro-hexopyranose [CPD:C21395]
Product
S-adenosyl-L-homocysteine [CPD:C00021];
dTDP-alpha-D-forosamine [CPD:C18034];
dTDP-4-(methylamino)-2,3,4,6-tetradeoxy-alpha-D-erythro-hexopyranose [CPD:C21395]
Comment
The enzyme was isolated from the bacterium Saccharopolyspora spinosa, where it is involved in the biosynthesis of spinosyn A, an active ingredient of several commercial insecticides.
History
EC 2.1.1.324 created 2016
Pathway
ec00523  Polyketide sugar unit biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K13330  dTDP-4-amino-2,3,4,6-tetradeoxy-D-glucose N,N-dimethyltransferase
Genes
SVU: B1H20_29845
APRE: CNX65_21845
AMI: Amir_4362
Reference
1  [PMID:18345667]
  Authors
Hong L, Zhao Z, Melancon CE 3rd, Zhang H, Liu HW
  Title
In vitro characterization of the enzymes involved in TDP-D-forosamine biosynthesis in the spinosyn pathway of Saccharopolyspora spinosa.
  Journal
J Am Chem Soc 130:4954-67 (2008)
DOI:10.1021/ja0771383
Other DBs
ExplorEnz - The Enzyme Database: 2.1.1.324
IUBMB Enzyme Nomenclature: 2.1.1.324
ExPASy - ENZYME nomenclature database: 2.1.1.324
BRENDA, the Enzyme Database: 2.1.1.324

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