KEGG   ENZYME: 2.1.1.340
Entry
EC 2.1.1.340                Enzyme                                 

Name
3-aminomethylindole N-methyltransferase;
NMT (gene name)
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
Sysname
S-adenosyl-L-methionine:3-(aminomethyl)indole N-methyltransferase (gramine-forming)
Reaction(IUBMB)
2 S-adenosyl-L-methionine + 3-(aminomethyl)indole = 2 S-adenosyl-L-homocysteine + gramine (overall reaction) [RN:R11697];
(1a) S-adenosyl-L-methionine + 3-(aminomethyl)indole = S-adenosyl-L-homocysteine + (1H-indol-3-yl)-N-methylmethanamine [RN:R11698];
(1b) S-adenosyl-L-methionine + (1H-indol-3-yl)-N-methylmethanamine = S-adenosyl-L-homocysteine + gramine [RN:R11699]
Reaction(KEGG)
Substrate
S-adenosyl-L-methionine [CPD:C00019];
3-(aminomethyl)indole [CPD:C21601];
(1H-indol-3-yl)-N-methylmethanamine [CPD:C21602]
Product
S-adenosyl-L-homocysteine [CPD:C00021];
gramine [CPD:C08304];
(1H-indol-3-yl)-N-methylmethanamine [CPD:C21602]
Comment
The enzyme, characterized from Hordeum vulgare (barley), catalyses two successive N-methylation reactions during the biosynthesis of gramine, a toxic indole alkaloid.
History
EC 2.1.1.340 created 2017
Orthology
K21937  3-aminomethylindole N-methyltransferase
Reference
1  [PMID:16664431]
  Authors
Leland TJ, Hanson AD
  Title
Induction of a Specific N-Methyltransferase Enzyme by Long-Term Heat Stress during Barley Leaf Growth.
  Journal
Plant Physiol 79:451-7 (1985)
DOI:10.1104/pp.79.2.451
Reference
2  [PMID:16930646]
  Authors
Larsson KA, Zetterlund I, Delp G, Jonsson LM
  Title
N-Methyltransferase involved in gramine biosynthesis in barley: cloning and characterization.
  Journal
Phytochemistry 67:2002-8 (2006)
DOI:10.1016/j.phytochem.2006.06.036
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 2.1.1.340
IUBMB Enzyme Nomenclature: 2.1.1.340
ExPASy - ENZYME nomenclature database: 2.1.1.340
BRENDA, the Enzyme Database: 2.1.1.340

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