KEGG   ENZYME: 2.1.1.342
Entry
EC 2.1.1.342                Enzyme                                 
Name
anaerobilin synthase;
chuW (gene name)
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
Sysname
S-adenosyl-L-methionine:protoheme C-methyltransferase (anaerobilin-producing)
Reaction(IUBMB)
2 S-adenosyl-L-methionine + protoheme + 2 reduced flavodoxin = S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + anaerobilin + Fe2+ + 2 oxidized flavodoxin [RN:R11700]
Reaction(KEGG)
R11700
Substrate
S-adenosyl-L-methionine [CPD:C00019];
protoheme [CPD:C00032];
reduced flavodoxin [CPD:C02745]
Product
S-adenosyl-L-homocysteine [CPD:C00021];
L-methionine [CPD:C00073];
5'-deoxyadenosine [CPD:C05198];
anaerobilin [CPD:C21603];
Fe2+ [CPD:C14818];
oxidized flavodoxin [CPD:C02869]
Comment
The enzyme, studied from the bacterium Escherichia coli O157:H7, is a radical SAM (AdoMet) enzyme that is involved in heme degradation and iron utilization under anaerobic conditions. The enzyme uses two SAM molecules for the reaction. The first molecule is used to generate a 5'-deoxyadenosyl radical, which abstracts a hydrogen atom from the methyl group of the second SAM molecule. The newly formed methylene radical attacks the substrate, causing a rearrangement of the porphyrin ring that results in the liberation of iron.
History
EC 2.1.1.342 created 2017
Orthology
K21936  anaerobilin synthase
Genes
ECEZ4914(chuW)
ECSECs_4383
ECFECH74115_4862
ETWECSP_4492(chuW)
ELXCDCO157_4120
ECOOECRM13514_4490(chuW)
ECOHECRM13516_4290(chuW)
ECGE2348C_3745(chuW)
EOKG2583_4238(chuW)
ELRECO55CA74_20280
ECPECP_3601
ENAECNA114_3622(chuW)
ECOSEC958_3906(chuW)
ECVAPECO1_2946(chuW)
ECMEcSMS35_3805
ECQECED1_4181
EUMECUMN_4003
ECTECIAI39_4005
EOCCE10_4046(chuW)
ECIUTI89_C4034(chuW)
EIHECOK1_3941
ECZECS88_3915
ECCc4314(chuW)
ELOEC042_3797(chuW)
ELNNRG857_17400
ESEECSF_3330
EABECABU_c39420
ELUUM146_17670
ELCi14_3982(chuW)
ELDi02_3982(chuW)
ELFLF82_543
ECOIECOPMV1_03832(hemN_2)
ECOJP423_19505
EALEAKF1_ch2447c
EMAC1192_15470
ESZFEM44_08635
SDZAsd1617_04688
KMIVW41_01195
RTGNCTC13098_05695(hemN_2)
REEelectrica_03842(hemN_2)
CKOCKO_02656
CBRAA6J81_10720
CYOCD187_17800
CPOTFOB25_05285
CFQC2U38_06080
CIFAL515_07175
CPARCUC49_02260
CITZE4Z61_11075
CNTJT31_10585
CEMLH23_07140
CENLH86_07090
CLAPNCTC11466_04200(hemN_3)
KIENCTC12125_00070(hemN_1)
KASKATP_10430
SYMK6K13_13825(hutW)
YREHEC60_09655
PSHISAMEA2665130_1272(hemN_1)
PSGCG163CM_23440(chuW)
EBFD782_3336
YPEYPO0286
YPKy0547
YPHYPC_0718
YPAYPA_3995
YPNYPN_3380
YPPYPDSF_3683
YPGYpAngola_A0654
YPZYPZ3_0247
YPTA1122_03695
YPDYPD4_0251
YPXYPD8_0254
YPWCH59_1575(hutW)
YPJCH55_3474(hutW)
YPVBZ15_3283(hutW)
YPLCH46_625(hutW)
YPSYPTB0344
YPOBZ17_2224(hutW)
YPIYpsIP31758_3797
YPYYPK_3885
YPBYPTS_0368
YPQDJ40_2064(hutW)
YPUBZ21_3716(hutW)
YPRBZ20_1767(hutW)
YPCBZ23_3986(hutW)
YPFBZ19_3816(hutW)
YENYE0335(hemN)
YEPYE105_C0363
YEYY11_35751
YELLC20_04852
YEWCH47_3139(hutW)
YETCH48_1255(hutW)
YEFFORC2_3744
YEEYE5303_30551(hemN)
YSIBF17_09835
YROCH64_2957(hutW)
YAKACZ76_10865
YMADA391_20705
YHID5F51_01930
YCAF0T03_01695
YMOHRD69_07440
SFWWN53_23055
SFGAV650_18870
SFOZ042_10400
RAHRahaq_4850
RACEJHW33_24980
RAAQ7S_24786
ROXBV494_22850
RVCJ9880_23930(hutW)
GQUAWC35_15525 AWC35_18220
ECAECA3009
PATREV46_14875
PATOGZ59_30160
PCTPC1_2750
PCCPCC21_028180
PCVBCS7_14055
PWAPecwa_1519
PPARA8F97_11170
PECW5S_1404
PWSA7983_00760
PPOABJK05_08985
PBRAB5S52_07750
PPUJE2566_14385
PARII2D83_07655
PAQUDMB82_0007525(hutW)
PVZOA04_30000(hutW)
PPAVLOZ86_07865(hutW)
BGJAWC36_03355
BRBEH207_11640
BNGEH206_07135
BIZHC231_07370
LBQCKQ53_04110
ETPLU633_22490(hutW)
KLNLH22_20160
MINTC7M51_02699
PVGCRN77_12965
PHAUPH4a_15265
PROTBTA34_07515
PCOLF1325_09310
PCIBF9282_09325
PSXDR96_3161(hutW)
PHEINCTC12003_00021(hemN_1)
EICNT01EI_1913
ETRETAE_1793(hemN1)
ETDETAF_1620(hutW)
ETEETEE_3837(hemN)
ETCETAC_08230
EDLAAZ33_09175(hutW)
EHOA9798_08465
HAVAT03_10710
HPARAL518_15120
OPODSM2777_16765
PRAGEKN56_03515
LRINCTC12151_01770(hemN_3)
HDUHD_0037(chuW)
GLECJD39_09425
MHTD648_1760
MHQD650_26390
MHATB824_24850
MHXMHH_c07140(hutW)
MHAEF382_07265
MHAMJ450_07765
MHAOJ451_08700
MHALN220_00820
MHAQWC39_13245
MHAYVK67_13250
MVIX808_20240
MVGX874_18970
MVEX875_1040
APLAPL_1523(chuW)
APJAPJL_1549(chuW)
APAAPP7_1583(chuW)
ASIASU2_10055
ASSASU1_10100
AEUACEE_10350
ALIGNCTC10568_01864(hemN_2)
GANUMN179_00603
BTOWQG_6040
BTREF542_16010
BTRHF543_17700
BTRAF544_6360
VCHVC_A0909
VCFIR04_03985
VCSMS6_A0948
VCEVch1786_II0595
VCQEN18_02840
VCJVCD_000422
VCIO3Y_17763
VCOVC0395_0328(hutW)
VCRVC395_A0934(hutW)
VCMVCM66_A0869(hutW)
VCLVCLMA_B0672
VCXVAA049_2079(hutW)
VCZVAB027_1208(hutW)
VVUVV2_1615
VVYVVA0425
VVMVVMO6_03382
VVLVV93_v1c34110
VPAVPA0427
VPBVPBB_A0396
VPKM636_01405
VPFM634_24330
VPHVPUCM_21207
VHAVIBHAR_05245
VCAM892_25080
VAGN646_3082
VEXVEA_001356
VHRAL538_26545
VOWA9237_18670
VROBSZ04_06925
VSPVS_II0290
VFUvfu_B01288
VNIVIBNI_A2348(phuW)
VCYIX92_22195
VCTJV59_17400
VTUIX91_17895
VEUIXK98_01645
VSIMTO69_15560(hutW)
VMIAL543_00430
VBRA6E01_14505
VSCVSVS12_01181
VSHBSZ05_24720
VTAB1790
VAFD1115_20610
VNLD3H41_24440
VCCFAZ90_19785(hutW)
VASGT360_09280
VZIG5S32_12455
VNVIF132_02695
VGIMID13_17295(hutW)
VFIVF_1226(hutW)
VFMVFMJ11_1306
VSAVSAL_I1750(phuW)
AWDAWOD_I_1443(phuW)
PPRPBPRA2102(Y0547)
PGBH744_2c2296
PDSCAY62_05885
PGHFH974_09230
GHOAL542_02035(hutW)
SALYE8E00_06055
SCOTHBA18_07640
PCIPCH70_51090
PCGAXG94_10560
EMODM558_07035
SPLSpea_3339
SHLShal_3411
SWPswp_3985
SVOSVI_3565
SJASJ2017_0135
SPSWSps_04905
SALGBS332_21045
SEURFM038_25965(hutW)
AGZM0C34_06620(hutW)
FBLFbal_0997 Fbal_3339
FESHER31_04505
MVSMVIS_2107(phuW)
ALVAlvin_1684
HHAHhal_0384
HHCM911_15930
EBSECTOBSL9_2338
RFOREIFOR_01945
ENWMJO57_27735(hutW)
AHAAHA_1982
AHYAHML_13035
AHDAI20_07140
AHRV428_13490
AHPV429_13510
AHJV469_09610
AHHRY45_12760
AHIVU14_09540
AAJBOQ57_12580
ASAASA_1862(hugW)
AEOO23A_p1153
AVRB565_2249
AVOAMS64_11005
AMEDB224_2619
ASRWL1483_3905
ADHCK627_13735
ACAVVI35_09760
AEMCK911_08285
AEAC2U39_09635
ARVC7N77_15435
AESC2U30_04090
AELNCTC12917_01871(hugW)
AALLI6G90_06845
AJDI6H43_20170
ARIVKYK33_10530(hutW)
GAPGAPWK_1068
FPPFPB0191_00913
NELNELON_08055
NSIA6J88_03965
NMJNM96_12135
NEKCGZ77_10595
NFVFAH67_10645
NSFFAH66_04115
NBLGJV52_04285
NANINCTC12227_01725(hemN_3)
NBCH3L91_09255
NSCJ7445_10855
NPFLPB400_00330(hutW)
NMBMON40_11540(hutW)
SALVSALWKB2_1968
KKIKKKWG1_0582(chuW)
ECORSAMEA4412678_0282(hemN_1)
EEXEZJ17_07035
MCERMON37_00250(hutW)
IODEJO50_01135
LHKLHK_01195(chuW)
JEUBJP62_00550
BTEBTH_II0299
BTQBTQ_3593
BTJBTJ_4629
BTZBTL_5417
BTDBTI_4964
BTVBTHA_4786
BTHEBTN_5087
BTHMBTRA_5355
BTHADR62_5217
BTHLBG87_5423
BUUWS70_27625
BULBW21_4493
BUDAQ610_30660
PTROG5S35_31695
JABVN23_01425
OFOBRW83_1102(hemN_3)
SUTKFG381_10915 FG381_10980
SWSI6J16_02115 I6J16_02120
BBAYA4V04_09395
CCVCCV52592_0647
CGRACGRAC_0445
CURECUREO_0226
CSPFCSF_0147
CPINCPIN18020_0552
CRXCRECT_0675
CGEOCGEO_1817
CBLACBLAS_1662
CMUCCMCT_1442
CSHOCSHOW_1424
DOAAXF15_02795
DOGHP555_07135
DOVDSCO28_24980
BBAEFRD01_15005
RPARPA2122
RPBRPB_0164
RPCRPC_1971
RPDRPD_2352
RPERPE_3151
RPTRpal_2413
RPXRpdx1_3405
HDNHden_0599
HNIW911_02640
RVARvan_2515
BVRBVIR_1164
BLAGBLTE_15640(hemN_1)
PSFPSE_0684(hemN)
RCPRCAP_rcc00151(hemN1)
RBLB6K69_04610
RSUNHU_00565
RHMB5V46_15110
RCERC1_3842(hemN)
RPMRSPPHO_02607(hemN)
BXIBK049_16365
BALTCFN77_11320
BKOCKF48_23135
TUMCBW65_12540 CBW65_12545
TABCIG75_15975 CIG75_15980
CBECbei_0683
CBZCbs_0683
ACELacsn021_02700
DLOK5I24_13355
SGYSgly_1484
KPARJL105_07965
CAZOG3A45_11385
VPRVpar_1337
VATB7L28_06765
VRM44547418_01417(hemN_2)
VDNNCTC11831_00486(hemN_1)
VNKVEIT17_04790
MHWACT01_05170
MEGDKB62_01995
MMAXLCQ47_11455(hutW)
DPNBCB69_06120
DHODia5BBH33_18270
SSGSelsp_1717
SRISELR_11700
SELEADJ74_05930
SELOAXE86_00725 AXE86_03655
SELTBCS37_01770
MHGMHY_26410
MFUNGXM21_08215(hutW)
PFTJBW_01206
STEDSPTER_02410 SPTER_41200
AINAcin_0814(hemN)
PFACPFJ30894_00785(hemN_2)
SDVBN159_2009
KBSEPA93_15615
OBTOPIT5_19630
RUFIK0V07_11075(hutW)
VBSEGM51_13195
PIRVN12_25605(hemN_3)
PBUL21SP3_01678(hemN_2)
VBCC5Q97_14580
TPEDTPE_1646
SSMSpirs_1131
SLRL21SP2_0606
FVAFV113G1_19070
FULC4N20_14530 C4N20_14555
FMOC4N19_06045
LEOAMK43_02430
LHFJCM16775_0691
LHGJMUB5056_0559
LTEJMUB4039_1845
LGOJCM16774_1764
STRSterm_0588
TLETlet_1247
DINSelin_2025
DAPDacet_0823
GTLEP073_08435(hutW)
TEUTEU_08400
TICFH039_06835
 » show all
Reference
1  [PMID:27791000]
  Authors
LaMattina JW, Nix DB, Lanzilotta WN
  Title
Radical new paradigm for heme degradation in Escherichia coli O157:H7.
  Journal
Proc Natl Acad Sci U S A 113:12138-12143 (2016)
DOI:10.1073/pnas.1603209113
Reference
2  [PMID:28045510]
  Authors
LaMattina JW, Delrossi M, Uy KG, Keul ND, Nix DB, Neelam AR, Lanzilotta WN
  Title
Anaerobic Heme Degradation: ChuY Is an Anaerobilin Reductase That Exhibits Kinetic Cooperativity.
  Journal
Biochemistry 56:845-855 (2017)
DOI:10.1021/acs.biochem.6b01099
Other DBs
ExplorEnz - The Enzyme Database: 2.1.1.342
IUBMB Enzyme Nomenclature: 2.1.1.342
ExPASy - ENZYME nomenclature database: 2.1.1.342
BRENDA, the Enzyme Database: 2.1.1.342

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