KEGG   ENZYME: 2.1.1.342Help
Entry
EC 2.1.1.342                Enzyme                                 

Name
anaerobilin synthase;
chuW (gene name)
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
BRITE hierarchy
Sysname
S-adenosyl-L-methionine:protoheme C-methyltransferase (anaerobilin-producing)
Reaction(IUBMB)
2 S-adenosyl-L-methionine + protoheme + 2 reduced flavodoxin = S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + anaerobilin + Fe2+ + 2 oxidized flavodoxin [RN:R11700]
Reaction(KEGG)
Substrate
S-adenosyl-L-methionine [CPD:C00019];
protoheme [CPD:C00032];
reduced flavodoxin [CPD:C02745]
Product
S-adenosyl-L-homocysteine [CPD:C00021];
L-methionine [CPD:C00073];
5'-deoxyadenosine [CPD:C05198];
anaerobilin [CPD:C21603];
Fe2+ [CPD:C14818];
oxidized flavodoxin [CPD:C02869]
Comment
The enzyme, studied from the bacterium Escherichia coli O157:H7, is a radical SAM (AdoMet) enzyme that is involved in heme degradation and iron utilization under anaerobic conditions. The enzyme uses two SAM molecules for the reaction. The first molecule is used to generate a 5'-deoxyadenosyl radical, which abstracts a hydrogen atom from the methyl group of the second SAM molecule. The newly formed methylene radical attacks the substrate, causing a rearrangement of the porphyrin ring that results in the liberation of iron.
History
EC 2.1.1.342 created 2017
Orthology
K21936  anaerobilin synthase
Genes
ECE: Z4914(chuW)
ECS: ECs4383
ECF: ECH74115_4862
ETW: ECSP_4492(chuW)
ELX: CDCO157_4120
ECOO: ECRM13514_4490(chuW)
ECOH: ECRM13516_4290(chuW)
ECG: E2348C_3745(chuW)
EOK: G2583_4238(chuW)
ELR: ECO55CA74_20280
ECC: c4314(chuW)
ECP: ECP_3601
ECI: UTI89_C4034(chuW)
ECV: APECO1_2946(chuW)
EOC: CE10_4046(chuW)
ELO: EC042_3797(chuW)
ESE: ECSF_3330
ENA: ECNA114_3622(chuW)
ELC: i14_3982(chuW)
ELD: i02_3982(chuW)
ELF: LF82_543
ECOI: ECOPMV1_03832(hemN_2)
ECOJ: P423_19505
ECOS: EC958_3906(chuW)
CKO: CKO_02656
RTG: NCTC13098_05695(hemN_2)
REE: electrica_03842(hemN_2)
CLAP: NCTC11466_04200(hemN_3)
KIE: NCTC12125_00070(hemN_1)
EBF: D782_3336
YPE: YPO0286
YPK: y0547
YPH: YPC_0718
YPA: YPA_3995
YPN: YPN_3380
YPZ: YPZ3_0247
YPD: YPD4_0251
YPX: YPD8_0254
YPW: CH59_1575(hutW)
YPJ: CH55_3474(hutW)
YPV: BZ15_3283(hutW)
YPL: CH46_625(hutW)
YPS: YPTB0344
YPO: BZ17_2224(hutW)
YPY: YPK_3885
YPB: YPTS_0368
YPQ: DJ40_2064(hutW)
YPU: BZ21_3716(hutW)
YPR: BZ20_1767(hutW)
YPC: BZ23_3986(hutW)
YPF: BZ19_3816(hutW)
YEN: YE0335(hemN)
YEY: Y11_35751
YEW: CH47_3139(hutW)
YET: CH48_1255(hutW)
YEE: YE5303_30551(hemN)
YRO: CH64_2957(hutW)
RAA: Q7S_24786
ECA: ECA3009
PATR: EV46_14875
PATO: GZ59_30160
PCT: PC1_2750
PEC: W5S_1404
PHAU: PH4a_15265
PSX: DR96_3161(hutW)
PHEI: NCTC12003_00021(hemN_1)
ETR: ETAE_1793(hemN1)
ETD: ETAF_1620(hutW)
ETE: ETEE_3837(hemN)
LRI: NCTC12151_01770(hemN_3)
PSHI: SAMEA2665130_1272(hemN_1)
HDU: HD_0037(chuW)
MHT: D648_1760
MHAT: B824_24850
MHX: MHH_c07140(hutW)
MHAE: F382_07265
MHAM: J450_07765
MHAO: J451_08700
MHAL: N220_00820
MHAQ: WC39_13245
MHAY: VK67_13250
MVE: X875_1040
APL: APL_1523(chuW)
APJ: APJL_1549(chuW)
APA: APP7_1583(chuW)
BTO: WQG_6040
BTRE: F542_16010
BTRH: F543_17700
BTRA: F544_6360
VCH: VCA0909
VCS: MS6_A0948
VCI: O3Y_17763
VCO: VC0395_0328(hutW)
VCR: VC395_A0934(hutW)
VCM: VCM66_A0869(hutW)
VCX: VAA049_2079(hutW)
VCZ: VAB027_1208(hutW)
VVU: VV2_1615
VVY: VVA0425
VPA: VPA0427
VAG: N646_3082
VSP: VS_II0290
VNI: VIBNI_A2348(phuW)
VTA: B1790
VFI: VF_1226(hutW)
VSA: VSAL_I1750(phuW)
AWD: AWOD_I_1443(phuW)
PPR: PBPRA2102(Y0547)
GHO: AL542_02035(hutW)
SPL: Spea_3339
SHL: Shal_3411
SWP: swp_3985
SVO: SVI_3565
SPSW: Sps_04905
MVS: MVIS_2107(phuW)
HHA: Hhal_0384
AHA: AHA_1982
ASA: ASA_1862(hugW)
AVR: B565_2249
AMED: B224_2619
ACAV: VI35_09760
KKI: KKKWG1_0582(chuW)
ECOR: SAMEA4412678_0282(hemN_1)
LHK: LHK_01195(chuW)
OFO: BRW83_1102(hemN_3)
CGRA: CGRAC_0445
CURE: CUREO_0226
CSPF: CSF_0147
RPA: RPA2122
RPB: RPB_0164
RPC: RPC_1971
RPD: RPD_2352
RPE: RPE_3151
RPT: Rpal_2413
HDN: Hden_0599
RVA: Rvan_2515
BVR: BVIR_1164
BLAG: BLTE_15640(hemN_1)
RCP: RCAP_rcc00151(hemN1)
PSF: PSE_0684(hemN)
RSU: NHU_00565
RCE: RC1_3842(hemN)
RPM: RSPPHO_02607(hemN)
VPR: Vpar_1337
VRM: 44547418_01417(hemN_2)
VDN: NCTC11831_00486(hemN_1)
MHG: MHY_26410
PFT: JBW_01206
AIN: Acin_0814(hemN)
PFAC: PFJ30894_00785(hemN_2)
PBU: L21SP3_01678(hemN_2)
GTL: EP073_08435(hutW)
TEU: TEU_08400
 » show all
Taxonomy
Reference
1  [PMID:27791000]
  Authors
LaMattina JW, Nix DB, Lanzilotta WN
  Title
Radical new paradigm for heme degradation in Escherichia coli O157:H7.
  Journal
Proc Natl Acad Sci U S A 113:12138-12143 (2016)
DOI:10.1073/pnas.1603209113
Reference
2  [PMID:28045510]
  Authors
LaMattina JW, Delrossi M, Uy KG, Keul ND, Nix DB, Neelam AR, Lanzilotta WN
  Title
Anaerobic Heme Degradation: ChuY Is an Anaerobilin Reductase That Exhibits Kinetic Cooperativity.
  Journal
Biochemistry 56:845-855 (2017)
DOI:10.1021/acs.biochem.6b01099
Other DBs
ExplorEnz - The Enzyme Database: 2.1.1.342
IUBMB Enzyme Nomenclature: 2.1.1.342
ExPASy - ENZYME nomenclature database: 2.1.1.342
BRENDA, the Enzyme Database: 2.1.1.342

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