KEGG   ENZYME: 2.1.1.343Help
Entry
EC 2.1.1.343                Enzyme                                 

Name
8-amino-8-demethylriboflavin N,N-dimethyltransferase;
rosA (gene name)
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
BRITE hierarchy
Sysname
S-adenosyl-L-methionine:8-amino-8-demethylriboflavin N,N-dimethyltransferase
Reaction(IUBMB)
2 S-adenosyl-L-methionine + 8-amino-8-demethylriboflavin = 2 S-adenosyl-L-homocysteine + roseoflavin (overall reaction) [RN:R11778];
(1a) S-adenosyl-L-methionine + 8-amino-8-demethylriboflavin = S-adenosyl-L-homocysteine + 8-demethyl-8-(methylamino)riboflavin [RN:R11779];
(1b) S-adenosyl-L-methionine + 8-demethyl-8-(methylamino)riboflavin = S-adenosyl-L-homocysteine + roseoflavin [RN:R11780]
Reaction(KEGG)
Substrate
S-adenosyl-L-methionine [CPD:C00019];
8-amino-8-demethylriboflavin [CPD:C21646];
8-demethyl-8-(methylamino)riboflavin [CPD:C21648]
Product
S-adenosyl-L-homocysteine [CPD:C00021];
roseoflavin [CPD:C21647];
8-demethyl-8-(methylamino)riboflavin [CPD:C21648]
Comment
The enzyme, characterized from the soil bacterium Streptomyces davawensis, catalyses the last two steps in the biosynthesis of the antibiotic roseoflavin.
History
EC 2.1.1.343 created 2017
Pathway
ec00998  Biosynthesis of secondary metabolites - other antibiotics
Orthology
K22269  8-amino-8-demethylriboflavin N,N-dimethyltransferase
Genes
SDV: BN159_8032(rosA)
Taxonomy
Reference
1  [PMID:21911488]
  Authors
Jankowitsch F, Kuhm C, Kellner R, Kalinowski J, Pelzer S, Macheroux P, Mack M
  Title
A novel N,N-8-amino-8-demethyl-D-riboflavin Dimethyltransferase (RosA) catalyzing the two terminal steps of roseoflavin biosynthesis in Streptomyces davawensis.
  Journal
J Biol Chem 286:38275-85 (2011)
DOI:10.1074/jbc.M111.292300
  Sequence
Reference
2  [PMID:26913589]
  Authors
Tongsook C, Uhl MK, Jankowitsch F, Mack M, Gruber K, Macheroux P
  Title
Structural and kinetic studies on RosA, the enzyme catalysing the methylation of  8-demethyl-8-amino-d-riboflavin to the antibiotic roseoflavin.
  Journal
FEBS J 283:1531-49 (2016)
DOI:10.1111/febs.13690
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 2.1.1.343
IUBMB Enzyme Nomenclature: 2.1.1.343
ExPASy - ENZYME nomenclature database: 2.1.1.343
BRENDA, the Enzyme Database: 2.1.1.343

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