KEGG   ENZYME: 2.1.1.349Help
Entry
EC 2.1.1.349                Enzyme                                 

Name
toxoflavin synthase;
toxA (gene name)
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
BRITE hierarchy
Sysname
S-adenosyl-L-methionine:1,6-didemethyltoxoflavin N1,N6-dimethyltransferase (toxoflavin-forming)
Reaction(IUBMB)
(1) S-adenosyl-L-methionine + 1,6-didemethyltoxoflavin = S-adenosyl-L-homocysteine + reumycin [RN:R12157];
(2) S-adenosyl-L-methionine + reumycin = S-adenosyl-L-homocysteine + toxoflavin [RN:R12158]
Reaction(KEGG)
Substrate
S-adenosyl-L-methionine [CPD:C00019];
1,6-didemethyltoxoflavin [CPD:C21956];
reumycin [CPD:C21957]
Product
S-adenosyl-L-homocysteine [CPD:C00021];
reumycin [CPD:C21957];
toxoflavine [CPD:C16789]
Comment
The enzyme is a dual-specificity methyltransferase that catalyses the last two steps of toxoflavin biosynthesis. Toxoflavin is a major virulence factor of several bacterial crop pathogens.
History
EC 2.1.1.349 created 2018
Orthology
K20331  toxoflavin synthase
Genes
PLU: plu0250
PLUM: A4R40_01225
PAY: PAU_03241
PTT: VY86_11790
XBV: XBW1_0361 XBW1_0364 XBW1_3521
XNE: XNC1_1384 XNC1_2445
XNM: XNC2_1346 XNC2_2352
XDO: XDD1_1102(toxA) XDD1_1316 XDD1_1320 XDD1_1508
XPO: XPG1_2381 XPG1_2700
XHO: A9255_09690 A9255_16450
PFL: PFL_1036
PPRC: PFLCHA0_c10560(toxA)
PPRO: PPC_1074
BGU: KS03_5278
BGO: BM43_1844
CCX: COCOR_03369(ubiG4)
CCRO: CMC5_053700(ubiE)
MSC: BN69_1794
SCT: SCAT_5482
SALS: SLNWT_7298
STRE: GZL_00343
SLD: T261_1553
SRW: TUE45_01220(ubiG_1)
SFK: KY5_0115
KSK: KSE_73110
 » show all
Taxonomy
Reference
1  [PMID:27070241]
  Authors
Fenwick MK, Philmus B, Begley TP, Ealick SE
  Title
Burkholderia glumae ToxA Is a Dual-Specificity Methyltransferase That Catalyzes the Last Two Steps of Toxoflavin Biosynthesis.
  Journal
Biochemistry 55:2748-59 (2016)
DOI:10.1021/acs.biochem.6b00167
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 2.1.1.349
IUBMB Enzyme Nomenclature: 2.1.1.349
ExPASy - ENZYME nomenclature database: 2.1.1.349
BRENDA, the Enzyme Database: 2.1.1.349

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