KEGG   ENZYME: 2.1.1.359
Entry
EC 2.1.1.359                Enzyme                                 

Name
[histone H3]-lysine36 N-trimethyltransferase;
SET2 (gene name);
KMT3A (gene name)
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
Sysname
S-adenosyl-L-methionine:[histone H3]-L-lysine36 N6-trimethyltransferase
Reaction(IUBMB)
3 S-adenosyl-L-methionine + a [histone H3]-L-lysine36 = 3 S-adenosyl-L-homocysteine + a [histone H3]-N6,N6,N6-trimethyl-L-lysine36 (overall reaction);
(1a) S-adenosyl-L-methionine + a [histone H3]-L-lysine36 = S-adenosyl-L-homocysteine + a [histone H3]-N6-methyl-L-lysine36;
(1b) S-adenosyl-L-methionine + a [histone H3]-N6-methyl-L-lysine36 = S-adenosyl-L-homocysteine + a [histone H3]-N6,N6-dimethyl-L-lysine36;
(1c) S-adenosyl-L-methionine + a [histone H3]-N6,N6-dimethyl-L-lysine36 = S-adenosyl-L-homocysteine + a [histone H3]-N6,N6,N6-trimethyl-L-lysine36
Reaction(KEGG)
(other) R03875 R04866 R04867
Substrate
S-adenosyl-L-methionine [CPD:C00019];
[histone H3]-L-lysine36;
[histone H3]-N6-methyl-L-lysine36;
[histone H3]-N6,N6-dimethyl-L-lysine36
Product
S-adenosyl-L-homocysteine [CPD:C00021];
[histone H3]-N6,N6,N6-trimethyl-L-lysine36;
[histone H3]-N6-methyl-L-lysine36;
[histone H3]-N6,N6-dimethyl-L-lysine36
Comment
The enzyme, characterized from yeast and mammals, catalyses the successive methylation of lysine36 of histone H3 (H3K36), forming the trimethylated form. These modifications influence the binding of chromatin-associated proteins. The enzyme couples the methylation reactions with transcriptional elongation through an interaction with the large subunit of RNA polymerase II. cf. EC 2.1.1.357, [histone H3]-lysine36 N-dimethyltransferase.
History
EC 2.1.1.359 created 1976 as EC 2.1.1.43, modified 1982, modified 1983, part transferred 2019 to EC 2.1.1.359
Pathway
ec00310  Lysine degradation
ec01100  Metabolic pathways
Orthology
K11423  [histone H3]-lysine36 N-trimethyltransferase
K23700  [histone H3]-lysine36 N-trimethyltransferase
Genes
HSA: 29072(SETD2)
PTR: 460331(SETD2)
PPS: 100993006(SETD2)
GGO: 101132140(SETD2)
PON: 100433116(SETD2)
NLE: 100584442(SETD2)
MCC: 711825(SETD2)
MCF: 102121581(SETD2)
CSAB: 103227561(SETD2)
CATY: 105573623(SETD2)
PANU: 101026986(SETD2)
RRO: 104671311(SETD2)
RBB: 108517641(SETD2)
TFN: 117080326(SETD2)
PTEH: 111555466(SETD2)
CJC: 100411953(SETD2)
SBQ: 101034613(SETD2)
MMUR: 105874896(SETD2)
MMU: 235626(Setd2)
MCAL: 110301050(Setd2)
MPAH: 110328077(Setd2)
RNO: 316013(Setd2)
MCOC: 116072571(Setd2)
MUN: 110554986(Setd2)
CGE: 100765637(Setd2)
PLEU: 114705174(Setd2)
NGI: 103732034(Setd2)
CCAN: 109689470(Setd2)
TUP: 102477917(SETD2)
CFA: 476643(SETD2)
VVP: 112912390(SETD2)
VLG: 121494650(SETD2)
AML: 100466135(SETD2)
UMR: 103675118(SETD2)
UAH: 113256807(SETD2)
ORO: 101365495(SETD2)
ELK: 111144347
MPUF: 101681942(SETD2)
EJU: 114196503(SETD2)
MLX: 118005368(SETD2)
FCA: 101085029(SETD2)
PTG: 102955436(SETD2)
PPAD: 109247730(SETD2)
AJU: 106969033(SETD2)
HHV: 120229315(SETD2)
BTA: 539860(SETD2)
BOM: 102266643(SETD2)
BIU: 109575988(SETD2)
BBUB: 102393639(SETD2)
CHX: 102183113(SETD2)
OAS: 101114649(SETD2)
ODA: 120868180(SETD2)
SSC: 100623101(SETD2)
CFR: 102512396(SETD2)
CDK: 105103636(SETD2)
BACU: 102997833(SETD2)
LVE: 103086894(SETD2)
OOR: 101280364(SETD2) 101282703
DLE: 111174450(SETD2)
PCAD: 112064944(SETD2)
ECB: 100064888(SETD2)
EPZ: 103551032(SETD2)
EAI: 106823246(SETD2)
MYB: 102260404(SETD2)
MYD: 102758485(SETD2)
MMYO: 118668537(SETD2)
MNA: 107529409(SETD2)
HAI: 109376628(SETD2)
DRO: 112309866(SETD2)
SHON: 118976190(SETD2)
AJM: 119037871(SETD2)
MMF: 118633365(SETD2)
PALE: 102884085(SETD2)
RAY: 107509112(SETD2)
MJV: 108400455(SETD2)
TOD: 119259277(SETD2)
LAV: 100655356(SETD2)
TMU: 101343139
MDO: 100024848(SETD2)
SHR: 100931646(SETD2)
PCW: 110217008(SETD2)
OAA: 114816148(SETD2)
GGA: 420404(SETD2)
PCOC: 116238217(SETD2)
MGP: 100539457(SETD2)
CJO: 107308808(SETD2)
NMEL: 110393293(SETD2)
ACYG: 106046805(SETD2)
TGU: 100224614(SETD2)
LSR: 110476945(SETD2)
SCAN: 103813035(SETD2)
PMOA: 120510532(SETD2)
GFR: 102039322(SETD2)
FAB: 101814785(SETD2)
PHI: 102106397(SETD2)
PMAJ: 107215411(SETD2)
CCAE: 111925482(SETD2)
CCW: 104686490(SETD2)
ETL: 114070402(SETD2)
FPG: 101913975(SETD2)
FCH: 102053248(SETD2)
CLV: 102098192(SETD2)
EGZ: 104123260(SETD2)
NNI: 104019238(SETD2)
ACUN: 113476423(SETD2)
PADL: 103916778(SETD2)
ASN: 102378063(SETD2)
AMJ: 102567964(SETD2)
CPOO: 109306447(SETD2)
GGN: 109291156(SETD2)
PSS: 102460966(SETD2)
CMY: 102947543(SETD2)
CPIC: 101953039(SETD2)
TST: 117871919(SETD2)
CABI: 116819138(SETD2)
ACS: 100559621(setd2)
PVT: 110069987(SETD2)
PBI: 103055763(SETD2)
PMUR: 107286005(SETD2)
TSR: 106542655(SETD2)
PGUT: 117656347(SETD2)
PMUA: 114607160(SETD2)
ZVI: 118091669(SETD2)
GJA: 107120098(SETD2)
XLA: 108718681(setd2.L) 108719797(setd2.S)
XTR: 100492810(setd2)
DRE: 794199(setd2)
CCAR: 109063165
IPU: 108267177(setd2)
PHYP: 113542507(setd2)
AMEX: 103039344(setd2)
EEE: 113570706(setd2)
TRU: 101076040(setd2)
LCO: 104940331(setd2)
NCC: 104948745(setd2) 104957630
ELY: 117265736(setd2)
PLEP: 121945864(setd2)
SLUC: 116049433(setd2)
ECRA: 117945702(setd2)
PFLV: 114557737(setd2)
GAT: 120810650(setd2)
MSAM: 119897705(setd2)
CUD: 121513186(setd2)
MZE: 101471514(setd2)
ONL: 100689872(setd2)
OAU: 116325355(setd2)
OLA: 101163764(setd2)
OML: 112143951(setd2)
XMA: 102216591(setd2)
XCO: 114141991(setd2)
XHE: 116717187(setd2)
PRET: 103478611(setd2)
CVG: 107081814(setd2)
CTUL: 119772867(setd2)
NFU: 107395695(setd2)
KMR: 108241998(setd2)
ALIM: 106534862
AOCE: 111572050(setd2)
CSEM: 103388382(setd2)
POV: 109633212(setd2)
LCF: 108891907(setd2)
SDU: 111236791(setd2)
SLAL: 111652786(setd2)
XGL: 120783986(setd2)
HCQ: 109518019(setd2)
BPEC: 110171513(setd2)
MALB: 109967312(setd2)
OTW: 112243336
OMY: 110520688(setd2)
SALP: 112076057(setd2)
ELS: 105018749(setd2)
SFM: 108927425(setd2)
PKI: 111850417(setd2)
LOC: 102690042 102692025(setd2)
LCM: 102359121(SETD2)
CMK: 103190896(setd2)
RTP: 109914799(setd2)
BFO: 118405673
SCLV: 120341878
SPU: 578079
APLC: 110986919
SKO: 100369661
DME: Dmel_CG1716(Set2)
DER: 6551019
DSE: 6618681
DSI: Dsimw501_GD17127(Dsim_GD17127)
DAN: 6505186
DSR: 110182093
DPE: 6601763
DMN: 108156147
DWI: 6649260
MDE: 101890762
LCQ: 111681651
ACOZ: 120955503
AARA: 120901301
AAG: 5567362
CPII: 120415447
AME: 100578450
ACER: 108000008
BIM: 100746901
BTER: 100652142
CCAL: 108630194
OBB: 114872234
MGEN: 117221020
NMEA: 116425768
CGIG: 122402982
SOC: 105208079
MPHA: 105837114
AEC: 105144959
ACEP: 105622474
PBAR: 105424427
VEM: 105570676
HST: 105190387
DQU: 106746542
CFO: 105253156
FEX: 115241758
LHU: 105678859
OBO: 105278228
PCF: 106791962
NVI: 100123115
CSOL: 105368748
MDL: 103571067
TCA: 662406
DPA: 109538546
ATD: 109601740
AGB: 108904757
NVL: 108569124
APLN: 108732872
PPYR: 116170941
OTU: 111414067
BMOR: 101738271
BMAN: 114245967
MSEX: 115443705
BANY: 112054435
PMAC: 106718844
PPOT: 106101273
PXU: 106118300
PRAP: 110997817
ZCE: 119830521
HAW: 110373157
TNL: 113493169
PXY: 105386651
API: 100165877
DNX: 107165146
AGS: 114124926
RMD: 113558505
BTAB: 109043976
CLEC: 106666752
NLU: 111044513
ZNE: 110839808
CSEC: 111870608
FCD: 110842413
PVM: 113817071
HAME: 121869854
DSV: 119442541
RSAN: 119385912
RMP: 119165735
TUT: 107365578
DPTE: 113790692
CSCU: 111621622
BGT: 106059019
GAE: 121387655
PMAX: 117317293
NVE: 5515716
EPA: 110241079
ADF: 107347448
AMIL: 114957186
PDAM: 113664166
SPIS: 111325620
DGT: 114517689
HMG: 100200309
SCE: YJL168C(SET2)
ERC: Ecym_8108
KMX: KLMA_10383(SET2)
NCS: NCAS_0I02880(NCAS0I02880)
NDI: NDAI_0B00260(NDAI0B00260)
TPF: TPHA_0A02720(TPHA0A02720)
TBL: TBLA_0J01040(TBLA0J01040)
TDL: TDEL_0G02090(TDEL0G02090)
KAF: KAFR_0F01770(KAFR0F01770)
CAL: CAALFM_C210250CA(SET2)
SLB: AWJ20_4803(SET2)
NCR: NCU00269(set-2)
NTE: NEUTE1DRAFT122301(NEUTE1DRAFT_122301)
MGR: MGG_01661
SSCK: SPSK_04901
NHE: NECHADRAFT_106311(SDG2102)
MAW: MAC_05010
MAJ: MAA_03512
CMT: CCM_07296
BFU: BCIN_10g05250(Bcset2)
MBE: MBM_00950
ANI: AN8825.2
ANG: ANI_1_490154(An17g01825)
ABE: ARB_07003
TVE: TRV_02085
PTE: PTT_20031
ZTR: MYCGRDRAFT_20674(SDG2404)
SPO: SPAC29B12.02c(set2)
CNE: CNG03810
CNB: CNBG0940
TASA: A1Q1_01839
LBC: LACBIDRAFT_318928(SDG16202)
ABP: AGABI1DRAFT123473(AGABI1DRAFT_123473)
ABV: AGABI2DRAFT76781(AGABI2DRAFT_76781)
MGL: MGL_3860
MRT: MRET_0662
 » show all
Reference
1  [PMID:11839797]
  Authors
Strahl BD, Grant PA, Briggs SD, Sun ZW, Bone JR, Caldwell JA, Mollah S, Cook RG, Shabanowitz J, Hunt DF, Allis CD
  Title
Set2 is a nucleosomal histone H3-selective methyltransferase that mediates transcriptional repression.
  Journal
Mol Cell Biol 22:1298-306 (2002)
DOI:10.1128/mcb.22.5.1298-1306.2002
  Sequence
[sce:YJL168C]
Reference
2  [PMID:12917322]
  Authors
Landry J, Sutton A, Hesman T, Min J, Xu RM, Johnston M, Sternglanz R
  Title
Set2-catalyzed methylation of histone H3 represses basal expression of GAL4 in Saccharomyces cerevisiae.
  Journal
Mol Cell Biol 23:5972-8 (2003)
DOI:10.1128/mcb.23.17.5972-5978.2003
  Sequence
[sce:YJL168C]
Reference
3  [PMID:16087749]
  Authors
Morris SA, Shibata Y, Noma K, Tsukamoto Y, Warren E, Temple B, Grewal SI, Strahl BD
  Title
Histone H3 K36 methylation is associated with transcription elongation in Schizosaccharomyces pombe.
  Journal
Eukaryot Cell 4:1446-54 (2005)
DOI:10.1128/EC.4.8.1446-1454.2005
  Sequence
Reference
4  [PMID:20048053]
  Authors
Lin LJ, Minard LV, Johnston GC, Singer RA, Schultz MC
  Title
Asf1 can promote trimethylation of H3 K36 by Set2.
  Journal
Mol Cell Biol 30:1116-29 (2010)
DOI:10.1128/MCB.01229-09
  Sequence
[sce:YJL168C]
Reference
5  [PMID:15798214]
  Authors
Kizer KO, Phatnani HP, Shibata Y, Hall H, Greenleaf AL, Strahl BD
  Title
A novel domain in Set2 mediates RNA polymerase II interaction and couples histone H3 K36 methylation with transcript elongation.
  Journal
Mol Cell Biol 25:3305-16 (2005)
DOI:10.1128/MCB.25.8.3305-3316.2005
Reference
6  [PMID:19332550]
  Authors
Yuan W, Xie J, Long C, Erdjument-Bromage H, Ding X, Zheng Y, Tempst P, Chen S, Zhu B, Reinberg D
  Title
Heterogeneous nuclear ribonucleoprotein L Is a subunit of human KMT3a/Set2 complex required for H3 Lys-36 trimethylation activity in vivo.
  Journal
J Biol Chem 284:15701-7 (2009)
DOI:10.1074/jbc.M808431200
Reference
7  [PMID:22266761]
  Authors
Wagner EJ, Carpenter PB
  Title
Understanding the language of Lys36 methylation at histone H3.
  Journal
Nat Rev Mol Cell Biol 13:115-26 (2012)
DOI:10.1038/nrm3274
Other DBs
ExplorEnz - The Enzyme Database: 2.1.1.359
IUBMB Enzyme Nomenclature: 2.1.1.359
ExPASy - ENZYME nomenclature database: 2.1.1.359
BRENDA, the Enzyme Database: 2.1.1.359

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