KEGG   ENZYME: 2.1.1.386
Entry
EC 2.1.1.386                Enzyme                                 
Name
small RNA 2'-O-methyltransferase;
HENMT1 (gene name);
HEN1 (gene name)
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
Sysname
S-adenosyl-L-methionine:[sRNA]-3'-end ribonucleotide 2'-O-methyltransferase
Reaction(IUBMB)
S-adenosyl-L-methionine + an [sRNA]-3'-end ribonucleotide = S-adenosyl-L-homocysteine + an [sRNA]-3'-end 2'-O-methylated ribonucleotide
Substrate
S-adenosyl-L-methionine [CPD:C00019];
[sRNA]-3'-end ribonucleotide
Product
S-adenosyl-L-homocysteine [CPD:C00021];
[sRNA]-3'-end 2'-O-methylated ribonucleotide
Comment
The enzyme adds a 2'-O-methyl group to the ribose of the last nucleotide in several types of small RNAs (sRNAs), protecting the 3'-end of sRNAs from uridylation activity and subsequent degradation.
History
EC 2.1.1.386 created 2022
Orthology
K20798  small RNA 2'-O-methyltransferase
Genes
HSA113802(HENMT1)
PTR469399(HENMT1)
PPS100993522(HENMT1)
GGO101143778(HENMT1)
PON100433952(HENMT1)
NLE100601958(HENMT1)
HMH116469683(HENMT1)
MCC698896(HENMT1)
MCF102140180(HENMT1)
MTHB126929817
MNI105489218(HENMT1)
CSAB103224322(HENMT1)
CATY105594315(HENMT1)
PANU101026366(HENMT1)
TGE112626868(HENMT1)
MLEU105528919(HENMT1)
RRO104669860(HENMT1)
RBB108518817(HENMT1)
TFN117076509(HENMT1)
PTEH111529343(HENMT1)
CANG105516086(HENMT1)
CJC100396677(HENMT1)
SBQ101049183(HENMT1)
CIMI108283084(HENMT1)
CSYR103272183(HENMT1)
MMUR105864062(HENMT1)
LCAT123634974(HENMT1)
PCOQ105813729(HENMT1)
OGA100962018(HENMT1)
MMU66715(Henmt1)
MCAL110291572(Henmt1)
MPAH110320675(Henmt1)
RNO100363095(Henmt1)
MCOC116093278(Henmt1)
ANU117707818(Henmt1)
MUN110545540(Henmt1)
CGE100752202
MAUA101829687(Henmt1)
PROB127216839(Henmt1)
PLEU114699939(Henmt1)
MORG121450967(Henmt1)
MFOT126487905
AAMP119801194(Henmt1)
NGI103747858(Henmt1)
HGL101718353(Henmt1)
CPOC100726074(Henmt1)
CCAN109686486(Henmt1)
DORD105997490(Henmt1)
DSP122106751(Henmt1)
PLOP125364147(Henmt1)
NCAR124967518
MMMA107158564(Henmt1)
OCU100343820
OPI101523145 101530433(HENMT1)
TUP102497802(HENMT1)
GVR103585315(HENMT1)
CFA479920(HENMT1)
CLUD112640739(HENMT1)
VVP112918239(HENMT1)
VLG121472562 121481005 121486408(HENMT1)
NPO129520200(HENMT1)
AML100463908(HENMT1)
UMR103674979(HENMT1)
UAH113244946(HENMT1)
UAR123795129(HENMT1)
ELK111143570
LLV125098073
MPUF101689031(HENMT1)
MNP132002277(HENMT1)
MLK131810199(HENMT1)
NVS122900269(HENMT1)
ORO101378659(HENMT1) 105757520
EJU114199783 114208037(HENMT1)
ZCA113920575(HENMT1) 113936762
MLX118019057(HENMT1)
NSU110580614(HENMT1) 110580820
LWW102745187
FCA101087676(HENMT1)
PYU121027603(HENMT1)
PCOO112848682(HENMT1)
PBG122480868(HENMT1)
LRUF124515658
PTG102956307(HENMT1)
PPAD109271968(HENMT1)
PUC125912316
AJU106977529
HHV120247166(HENMT1)
BTA515853(HENMT1)
BOM102272255(HENMT1)
BIU109555994(HENMT1)
BBUB102413353(HENMT1)
BBIS104981698(HENMT1)
CHX102187549(HENMT1)
OAS101102695(HENMT1)
BTAX128045543(HENMT1)
ODA120856523(HENMT1)
CCAD122436606(HENMT1)
MBEZ129540588(HENMT1)
SSC100153448(HENMT1)
CFR102507237(HENMT1)
CBAI105070480(HENMT1)
CDK105085622(HENMT1)
VPC102527256(HENMT1)
BACU103017590(HENMT1)
LVE103087431(HENMT1)
OOR101275641(HENMT1)
DLE111180094(HENMT1)
PCAD102986509(HENMT1)
PSIU116756435(HENMT1)
NASI112397841(HENMT1)
ECB100060671(HENMT1)
EPZ103560937(HENMT1)
EAI106827422(HENMT1)
MYB102253748(HENMT1)
MYD102758654(HENMT1) 102773992
MMYO118673487(HENMT1)
MLF102440822(HENMT1)
MNA107541172(HENMT1)
PKL118727212(HENMT1)
EFUS103291323(HENMT1)
HAI109374301(HENMT1) 109391880
DRO112313442(HENMT1)
SHON118990063(HENMT1)
AJM119049920 119054543(HENMT1)
PDIC114489046(HENMT1)
PHAS123804372(HENMT1)
MMF118637569(HENMT1)
RFQ117015239(HENMT1)
PPAM129063697(HENMT1)
PALE102877855(HENMT1)
PGIG120584969(HENMT1)
PVP105297430(HENMT1)
RAY107518298(HENMT1)
MJV108406391(HENMT1)
TOD119236499(HENMT1)
SARA101553990(HENMT1)
LAV100662378(HENMT1)
TMU101357253
ETF101664404(HENMT1)
DNM101420556(HENMT1)
MDO100033025(HENMT1)
GAS123247118(HENMT1)
SHR100922735(HENMT1)
AFZ127559413
PCW110217854(HENMT1)
OAA100083579(HENMT1)
GGA429055(HENMT1)
PCOC116230583(HENMT1)
MGP100539667(HENMT1)
CJO107317122(HENMT1)
TPAI128072275(HENMT1)
LMUT125693704(HENMT1)
NMEL110402838(HENMT1)
APLA101793047(HENMT1)
ACYG106041998(HENMT1)
CATA118243480(HENMT1)
AFUL116491787(HENMT1)
TGU100229608(HENMT1)
LSR110479649(HENMT1)
SCAN103814849(HENMT1)
PMOA120510461(HENMT1)
OTC121344577(HENMT1)
PRUF121360092(HENMT1)
GFR102033331(HENMT1)
FAB101809338(HENMT1)
OMA130256495(HENMT1)
PHI102111143(HENMT1)
PMAJ107208029(HENMT1)
CCAE111932736(HENMT1)
CCW104695397(HENMT1)
CBRC103612563(HENMT1)
ETL114055868(HENMT1)
ZAB102072009(HENMT1)
ACHL103808860(HENMT1)
SVG106857335(HENMT1)
MMEA130572542(HENMT1)
HRT120756749(HENMT1)
FPG101913377(HENMT1)
FCH102057337(HENMT1)
CLV102087253(HENMT1)
EGZ104126480(HENMT1)
NNI104017631(HENMT1)
PCRI104023697(HENMT1)
PLET104624518
EHS104503269(HENMT1)
PCAO104041974(HENMT1)
ACUN113483011(HENMT1)
TALA104368648(HENMT1)
PADL103917113(HENMT1)
AFOR103902178(HENMT1)
ACHC115348804(HENMT1)
HALD104310371(HENMT1)
HLE104829282(HENMT1)
AGEN126041485
GCL127018834
CCRI104154585(HENMT1)
CSTI104549169(HENMT1)
CMAC104483998(HENMT1)
MUI104537602(HENMT1)
BREG104638602(HENMT1)
FGA104069262(HENMT1)
GSTE104255366(HENMT1)
LDI104351599(HENMT1)
MNB103779962(HENMT1)
OHA104326334(HENMT1)
NNT104413862(HENMT1)
SHAB115612184(HENMT1)
DPUB104300595(HENMT1)
PGUU104457660(HENMT1)
ACAR104520074(HENMT1)
CPEA104396778(HENMT1)
AVIT104266973(HENMT1)
CVF104286210(HENMT1)
RTD128913882(HENMT1)
CUCA104054768(HENMT1)
TEO104377825(HENMT1)
BRHI104491932(HENMT1)
AAM106499737(HENMT1)
AROW112976504(HENMT1)
NPD112951153(HENMT1)
TGT104569254(HENMT1)
DNE112994475(HENMT1)
SCAM104140821(HENMT1)
ASN102388692(HENMT1)
AMJ102569876(HENMT1)
CPOO109307034(HENMT1)
GGN109287111(HENMT1)
PSS102449045 102456236(HENMT1)
CMY102936026(HENMT1)
CCAY125640858(HENMT1)
DCC119859755(HENMT1)
CPIC101932933(HENMT1)
TST117881675(HENMT1)
CABI116838487(HENMT1)
MRV120370432(HENMT1)
ACS100565443(henmt1)
ASAO132774459(HENMT1)
PVT110074487(HENMT1)
SUND121929097(HENMT1)
PBI103055219(HENMT1)
PMUR107294764(HENMT1)
CTIG120301891(HENMT1)
TSR106545278(HENMT1)
PGUT117677287(HENMT1)
APRI131195131(HENMT1)
PTEX113436012(HENMT1)
NSS113414751(HENMT1)
VKO123024560(HENMT1)
PMUA114598682(HENMT1)
PRAF128415409(HENMT1)
ZVI118088476(HENMT1)
HCG128325018(HENMT1)
GJA107112066(HENMT1)
STOW125433002(HENMT1)
EMC129331341(HENMT1)
XLA108714449(henmt1.L)
XTR100286818(henmt1)
NPR108797404(HENMT1)
RTEM120945573(HENMT1)
BBUF120978764(HENMT1)
BGAR122943529(HENMT1)
MUO115472151(HENMT1)
GSH117346079 117346753 117352495
DRE550540(henmt1)
SRX107710559 107726462
SANH107696160(henmt1) 107698398
SGH107555628 107601273
CCAR109081378
CAUA113120802(henmt1)
CGIB127984177
PTET122324905(henmt1)
LROH127183483(henmt1)
OMC131526546(henmt1)
PPRM120493917(henmt1)
RKG130103077(henmt1)
MAMB125278179(henmt1)
CIDE127502802
MASI127410358 127415610
TROS130565687(henmt1)
TDW130434129(henmt1)
MANU129429555(henmt1)
IPU108257722
IFU128600967(henmt1)
PHYP113535153(henmt1)
SMEO124376746(henmt1)
TFD113647582(henmt1)
TVC132842391(henmt1)
AMEX103040529(henmt1)
CMAO118816126(henmt1)
EEE113590479(henmt1)
CHAR105910749(henmt1)
TRU101065700(henmt1)
TFS130529345(henmt1)
TNGGSTEN00004980G001
NCC104959867(henmt1)
TBEN117495893(henmt1)
CGOB115007033(henmt1)
PGEO117445408(henmt1)
GACU117542485(henmt1)
ELY117253438(henmt1)
EFO125895351(henmt1)
PLEP121940981(henmt1)
SLUC116038121(henmt1)
ECRA117950460(henmt1)
ESP116695003(henmt1)
PFLV114561917(henmt1)
GAT120824237(henmt1)
PPUG119216490(henmt1)
AFB129094680(henmt1)
CLUM117729365(henmt1)
MSAM119900348(henmt1)
SCHU122877671(henmt1)
CUD121512271(henmt1)
ALAT119028637(henmt1)
MZE101464633(henmt1)
ONL100705551(henmt1)
OAU116315479(henmt1)
OLA101160973(henmt1)
OML112150411(henmt1)
XMA102229164(henmt1)
XCO114150557(henmt1)
XHE116725994(henmt1)
PRET103463099(henmt1)
PFOR103155256(henmt1)
PLAI106955912(henmt1)
PMEI106903774(henmt1)
GAF122839055(henmt1)
PPRL129375720(henmt1)
CVG107096592(henmt1)
CTUL119789869(henmt1)
GMU124873355(henmt1)
NFU107392977(henmt1)
KMR108238396(henmt1)
ALIM106511703
NWH119430417(henmt1)
AOCE111566239 111574174 111588472
MCEP125009485(henmt1)
CSEM103396495(henmt1)
POV109643745(henmt1)
SSEN122761756 122781310
HHIP117760211(henmt1)
HSP118121210(henmt1)
LCF108880114
SDU111237579(henmt1)
SLAL111671748(henmt1)
XGL120790624(henmt1)
HCQ109512758(henmt1)
SSCV125974789
SBIA133503238(henmt1)
PEE133406339(henmt1)
PTAO133481012(henmt1)
BPEC110160602(henmt1)
MALB109969884(henmt1)
BSPL114853681(henmt1)
SJO128362280(henmt1)
SASA106560441(henmt1)
STRU115157899(henmt1)
OTW121838637(henmt1)
OMY110524704(henmt1)
OGO123996676(henmt1)
ONE115108636(henmt1)
OKI109903035(henmt1)
OKE118393655(henmt1)
SALP111976080(henmt1)
SNH120054578(henmt1)
CCLU121533840(henmt1)
ELS105009224(henmt1)
SFM108935560(henmt1)
PKI111840071(henmt1)
AANG118226378(henmt1)
LOC102685458(henmt1)
PSPA121326941(henmt1)
ARUT117416538(henmt1)
PSEX120514582(henmt1)
LCM102355311(HENMT1)
CMK103174758(henmt1)
RTP109931856(henmt1)
CPLA122555677(henmt1)
HOC132818880(henmt1)
LERI129703478(henmt1)
PMRN116944923(HENMT1)
LRJ133341645(HENMT1)
BFO118421638
BBEL109482505
CIN100184909
SCLV120338766
SPU589300
APLC110982946
SKO100373004
DMEDmel_CG12367(Hen1)
DER6546903
DSE6608761
DSIDsimw501_GD15398(Dsim_GD15398)
DYADyak_GE12407
DSR110177812
DPO4803379
DPE6590889
DMN108159530
DWI6652488
DGR6559433
DMODmoj_GI20134
DAZ108617288
DNV108654533
DHE111602284
CCAT101455564
BDR105230002
RZE108357294
TDA119680143
MDE101893186
SCAC106091514
LCQ111685052
LSQ119608074
GFS119639798
ECOE129952893
CLON129610941
HIS119661113
AGA1276331
ACOZ120948674
AARA120908429
AMER121591807
ASTE118512770 118516359
AMOU128304033
AAG5572757
AALB109407983 115254252
CQUCpipJ_CPIJ004597 CpipJ_CPIJ016924
CPII120420120
CNS116351595
BCOO119075134
AME408762
BIM100742065
BTER100646218
CCAL108625403
OBB114877614
SOC105202089
MPHA105835615
AEC105143370
ACEP105623050
PBAR105425518
VEM105562484
HST105192258
DQU106742963
CFO105253332
LHU105679398
PGC109854062
OBO105277818
PCF106788923
NVI100121690
CSOL105368784
MDL103574275
CGLO123271349
TCA656404
DPA109540160
ATD109600661
BMOR101739273
BMAN114245511
DPLKGM_213062
PMAC106719552
HAW110379170
TNL113494504
PXY105386545
API100158970 100162178
DNX107163582 107165993
AGS114119423 114124762
RMD113561029
ACOO126835484
DVT126894654
CLEC106666382
FCD110847061
PVM113808228
PJA122252554
PCHN125038491
PMOO119575272
HAME121866489 121874259
PCLA123771052 123771473
CQD128697986
PTRU123512059
HAZT108674284
LSM121122115
TCF131887716
DSV119432901 119433445
RSAN119404583
RMP119180560
TUT107366679
DPTE113788848
DFR124498126
CSCU111632518
PTEP107450143
UDV129234606
SDM118197773
LPOL106457090
PMEO129598024
HROHELRODRAFT_151325
LGILOTGIDRAFT_125700
PVUL126807940
PCAN112575122
BGT106066844
GAE121384441 121391382
HRF124144779
HRJ124278823
CRG105321056
CVN111124957 111124960
CANU128157548
OED125681833
MYI110452896
PMAX117322036
MMER123551984
RPHI132728314
OBI106872784
OSN115223334
LAK106170035
NVE5516223
EPA110242699
ATEN116297700
AMIL114953037
PDAM113671681
SPIS111338187
DGT114518822
XEN124439326
HMG100200697
HSY130626177
AQU100632519 100635584 100635710 109581380
ATHAT4G20910(HEN1) AT4G20920
ALY9303940 9303941
CRB17878985 17879935
CSAT104718246 104722971 104728186 104731463
EUSEUTSA_v10024326mg EUTSA_v10024347mg
BRP103858478 103858486 103858496
BNA106375681 106375682 106375683 106438472 106438482
BOE106295193 106295343 106306076
RSZ108842047 108842048 108842050
THJ104812246
CPAP110807609
CIT102613903
CICCICLE_v10014179mg
PVY116107394
MINC123218690
TCC18595070
GRA105773492
GHI107962954 121203428
GAB108486159
DZI111275381 111280713
EGR104427561
GMX100785461(HEN1B) 100815299(HEN1A)
GSJ114412642 114421457
PVUPHAVU_002G198200g
VRA106752789
VAR108319414
VUN114178875
VUM124840387
CCAJ109813215
APRC113858082
MTR25493375
TPRA123915689
CAM101499604
PSAT127101411
VVO131603081
LJALj4g3v0286120.1(Lj4g3v0286120.1)
ADU107481688
AIP107631547
AHF112734566 112792353
LANG109358783
PCIN129285021
FVE101294545
RCN112201437
PPER18778317
PMUM103325240
PAVI110758633
PDUL117624243
MDM103422322 103435631
MSYL126623890
PXB103952362
MNT21408661
CSAV115697939
CSV101215019
CMO103488472
BHJ120083182
MCHA111010738
CMAX111495285
CMOS111434192
CPEP111782911
RCU8265514
JCU105636843
HBR110648028
MESC110617394
POP18103519 7471687
PEU105134901 105136237
PALZ118029481 118035900
JRE109009829
CILL122276282
CAVE132177639
QSU112003620
QLO115989792
TWL119989754 120005082
VVI100259382
VRI117923955
SLY101264590
SPEN107011777
SOT102595903
SSTN125847827
SDUL129872267
CANN107859056
LBB132621324
NTA107768656 107779312
NSY104224851
NTO104114395
NAU109208676
INI109153043
ITR116028068
SIND105167253
OEU111410668
EGT105951328
SSPL121784631 121787672
APAN127254871
HAN110937278
ECAD122590532
LSV111908065
CCAV112526355
DCR108204981
CSIN114256732
RVL131309345 131315566
AEW130760441
BVG104901238
SOE110790263
CQI110686591 110692121
ATRI130823963
MOF131159400 131160852
NNU104603462
MING122094117
TSS122640529
PSOM113312641
OSA4342471
DOSAOs07t0164000-01(Os07g0164000)
OBR102713648
OGL127779707
BDI100832180
ATS109758170 109783374
TDC119354545 119363730
TAES123044943 123048735 123052816 123188691
TUA125537373
LPER127333868
LRD124678099
SBI8081159
ZMA103631972 103637342
SITA101755469
SVS117845163
PVIR120659404 120662623
PHAI112880741
PDA103723093
EGU105059809
MUS103998186
ZOF122009385 122017089
DCT110095826
PEQ110025826
AOF109819927
MSIN131244483
NCOL116264111
ATR18427258
SMOSELMODRAFT_418472 SELMODRAFT_423789
PPP112285869
SPOSPBC336.05c
CNECNC00420
CNBCNBC6810
CNGCNAG_03046
CGICGB_C9300C
TMSTREMEDRAFT_61075
CCACCcaHIS019_0104570(CcaverHIS019_0104570)
PPLPOSPLDRAFT_91319
TVSTRAVEDRAFT_37905
DSQDICSQDRAFT_144307
PCOPHACADRAFT_116997
SHSSTEHIDRAFT_137050
HIRHETIRDRAFT_237411
PSQPUNSTDRAFT_25427
ADLAURDEDRAFT_188426
FMEFOMMEDRAFT_76349
GTRGLOTRDRAFT_78370
RSXRhiXN_03164
LBCLACBIDRAFT_318056
CCICC1G_06330
ABPAGABI1DRAFT75365(AGABI1DRAFT_75365)
ABVAGABI2DRAFT177408(AGABI2DRAFT_177408)
MPRMPER_02184
MRRMoror_12914
MOREE1B28_011076
SCMSCHCO_01107153(SCHCODRAFT_01107153)
CPUTCONPUDRAFT_163412
SLASERLADRAFT_438057
WICJ056_001751
UMAUMAG_06038
PFPPFL1_02029
SGRAEX895_003521
PGRPGTG_03165
PTRCPtA15_1A869
PSTRPst134EA_000207
ACANACA1_362270
TETTTHERM_00433810
PTMGSPATT00036636001
TVATVAG_2v0181900
MSRAU15_02555
LALAT746_19155
HMIsoil367_08650
KIMG3T16_01260
MEJQ7A_151
EBSECTOBSL9_2237
NAXHC341_05785
WMAWM2015_16
HCOLOKO_01816
HSIBOX17_04235
HAGBB497_07150
HBPHPTD01_1980
AXXERS451415_05800
RFRRfer_3568
ATWC0099_00300
RBMTEF_20920
RMMROSMUCSMR3_02675
ROKRAK1035_0500
LVSLOKVESSMR4R_01910
RBGBG454_13300
HDHG5B40_10045
GAKX907_2149
GSBGSUB_00220
DESDSOUD_2125
LWIUE46_16120
BBEBBR47_27360
BFMBP422_08880
BAGRBA6348_15500
PJDPjdr2_2518
PDUPDUR_03900
PODPODO_01030
PBKBack11_36150
STRSterm_3123
 » show all
Reference
1  [PMID:12225663]
  Authors
Park W, Li J, Song R, Messing J, Chen X.
  Title
CARPEL FACTORY, a Dicer homolog, and HEN1, a novel protein, act in microRNA metabolism in Arabidopsis thaliana.
  Journal
Curr Biol 12:1484-95 (2002)
DOI:10.1016/s0960-9822(02)01017-5
Reference
2  [PMID:15705854]
  Authors
Yu B, Yang Z, Li J, Minakhina S, Yang M, Padgett RW, Steward R, Chen X.
  Title
Methylation as a crucial step in plant microRNA biogenesis.
  Journal
Science 307:932-5 (2005)
DOI:10.1126/science.1107130
Reference
3  [PMID:18029764]
  Authors
Kirino Y, Mourelatos Z
  Title
2'-O-methyl modification in mouse piRNAs and its methylase.
  Journal
Nucleic Acids Symp Ser (Oxf) 417-8 (2007)
DOI:10.1093/nass/nrm209
Reference
4  [PMID:19812675]
  Authors
Huang Y, Ji L, Huang Q, Vassylyev DG, Chen X, Ma JB.
  Title
Structural insights into mechanisms of the small RNA methyltransferase HEN1.
  Journal
Nature 461:823-7 (2009)
DOI:10.1038/nature08433
  Sequence
[ath:AT4G20910]
Reference
5  [PMID:29703750]
  Authors
Peng L, Zhang F, Shang R, Wang X, Chen J, Chou JJ, Ma J, Wu L, Huang Y.
  Title
Identification of substrates of the small RNA methyltransferase Hen1 in mouse spermatogonial stem cells and analysis of its methyl-transfer domain.
  Journal
J Biol Chem 293:9981-9994 (2018)
DOI:10.1074/jbc.RA117.000837
  Sequence
[hsa:113802]
Other DBs
ExplorEnz - The Enzyme Database: 2.1.1.386
IUBMB Enzyme Nomenclature: 2.1.1.386
ExPASy - ENZYME nomenclature database: 2.1.1.386
BRENDA, the Enzyme Database: 2.1.1.386

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