KEGG   ENZYME: 2.1.1.41Help
Entry
EC 2.1.1.41                 Enzyme                                 

Name
sterol 24-C-methyltransferase;
Delta24-methyltransferase;
Delta24-sterol methyltransferase;
zymosterol-24-methyltransferase;
S-adenosyl-4-methionine:sterol Delta24-methyltransferase;
SMT1;
24-sterol C-methyltransferase;
S-adenosyl-L-methionine:Delta24(23)-sterol methyltransferase;
phytosterol methyltransferase
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
BRITE hierarchy
Sysname
S-adenosyl-L-methionine:zymosterol 24-C-methyltransferase
Reaction(IUBMB)
S-adenosyl-L-methionine + 5alpha-cholesta-8,24-dien-3beta-ol = S-adenosyl-L-homocysteine + 24-methylene-5alpha-cholest-8-en-3beta-ol [RN:R04427]
Reaction(KEGG)
R04427;
(other) R07481
Show
Substrate
S-adenosyl-L-methionine [CPD:C00019];
5alpha-cholesta-8,24-dien-3beta-ol [CPD:C05437]
Product
S-adenosyl-L-homocysteine [CPD:C00021];
24-methylene-5alpha-cholest-8-en-3beta-ol [CPD:C04525]
Comment
Requires glutathione. Acts on a range of sterols with a 24(25)-double bond in the sidechain. While zymosterol is the preferred substrate it also acts on desmosterol, 5alpha-cholesta-7,24-dien-3beta-ol, 5alpha-cholesta-5,7,24-trien-3beta-ol, 4alpha-methylzymosterol and others. S-Adenosyl-L-methionine attacks the Si-face of the 24(25) double bond and the C-24 hydrogen is transferred to C-25 on the Re face of the double bond.
History
EC 2.1.1.41 created 1972, modified 2001
Pathway
ec00100  Steroid biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
ec01130  Biosynthesis of antibiotics
Orthology
K00559  sterol 24-C-methyltransferase
Genes
BFO: BRAFLDRAFT_70257
NAI: NECAME_03546
AQU: 100639491
ATH: AT5G13710(SMT1)
ALY: ARALYDRAFT_909323
CRB: 17882375
CSAT: 104705624 104735382 104769590
EUS: EUTSA_v10014014mg
BRP: 103850891 103856028
BNA: 106371823 106384356 106392091 106431054 106440921
CPAP: 110809321
CIT: 102628258
GRA: 105790583
VRA: 106760797
VAR: 108332339
CAM: 101497864
LJA: Lj0g3v0067279.1(Lj0g3v0067279.1) Lj3g3v0825030.1(Lj3g3v0825030.1) Lj3g3v0825030.2(Lj3g3v0825030.2)
FVE: 101293342
PPER: 18785581
PMUM: 103330640
PAVI: 110765835
JCU: 105628730
SIND: 105169293
CCAV: 112522572
BVG: 104907757
SOE: 110778345
NNU: 104597375
DOSA: Os03t0807600-00(Os03g0807600) Os07t0206700-01(Os07g0206700) Os11t0296900-00(Os11g0296900)
ATS: 109734415(LOC109734415) 109756778(LOC109756778)
AOF: 109833715
SMO: SELMODRAFT_449609(SMT1-2) SELMODRAFT_449613(SMT1-1)
MIS: MICPUN_93596(SMT1)
SCE: YML008C(ERG6)
ERC: Ecym_4063
KMX: KLMA_30671(ERG6)
NCS: NCAS_0H02570(NCAS0H02570)
NDI: NDAI_0C01050(NDAI0C01050)
TPF: TPHA_0D02350(TPHA0D02350)
TBL: TBLA_0D01630(TBLA0D01630)
TDL: TDEL_0A03930(TDEL0A03930)
KAF: KAFR_0C05410(KAFR0C05410)
CAL: CAALFM_C302150CA(ERG6)
CDU: CD36_82130(ERG6)
CAUR: QG37_03583
SLB: AWJ20_2170(ERG6)
NCR: NCU02502 NCU03006(erg-4)
NTE: NEUTE1DRAFT150553(NEUTE1DRAFT_150553) NEUTE1DRAFT73978(NEUTE1DRAFT_73978)
MAW: MAC_08238
MBE: MBM_07594
ANG: ANI_1_256124(An14g01590) ANI_1_752184(An04g04210)
PTE: PTT_11273
SPO: SPBC16E9.05(erg6)
CNE: CNB03100
CNB: CNBB2600
LBC: LACBIDRAFT_184995(erg6)
ABP: AGABI1DRAFT115299(AGABI1DRAFT_115299)
ABV: AGABI2DRAFT194260(AGABI2DRAFT_194260)
MGL: MGL_2877
DDI: DDB_G0288907(smt1)
DFA: DFA_09943(smt1)
SMIN: v1.2.010320.t1(symbB.v1.2.010320.t1) v1.2.020967.t1(symbB.v1.2.020967.t1) v1.2.040208.t1(symbB.v1.2.040208.t1)
SPAR: SPRG_05001
TMC: LMI_2904
NDK: I601_4050(rebM_4)
 » show all
Taxonomy
Reference
1  [PMID:5354959]
  Authors
Moore JT, Gaylor JL.
  Title
Isolation and purification of an S-adenosylmethionine: delta 24-sterol methyltransferase from yeast.
  Journal
J Biol Chem 244:6334-40 (1969)
Reference
2  [PMID:8597586]
  Authors
Venkatramesh M, Guo DA, Jia Z, Nes WD.
  Title
Mechanism and structural requirements for transformation of substrates by the (S)-adenosyl-L-methionine:delta 24(25)-sterol methyl transferase from Saccharomyces cerevisiae.
  Journal
Biochim Biophys Acta 1299:313-24 (1996)
DOI:10.1016/0005-2760(95)00218-9
Reference
3
  Authors
Tong, Y., McCourt, B.S., Guo, D., Mangla, A.T., Zhou, W.X., Jenkins, M.D., Zhou, W., Lopez, M. and Nes, W.D.
  Title
, Stereochemical features of C-methylation on the path to Delta24(28)-methylene and Delta24(28)-ethylidene sterols: studies on the recombinant phytosterol methyl transferase from Arabidopsis thaliana.
  Journal
Tetrahedron Lett 38:6115-6118 (1997)
Reference
4  [PMID:9746350]
  Authors
Bouvier-Nave P, Husselstein T, Benveniste P.
  Title
Two families of sterol methyltransferases are involved in the first and the second methylation steps of plant sterol biosynthesis.
  Journal
Eur J Biochem 256:88-96 (1998)
DOI:10.1046/j.1432-1327.1998.2560088.x
  Sequence
[osa:4342690]
Reference
5  [PMID:9606964]
  Authors
Nes WD, McCourt BS, Zhou WX, Ma J, Marshall JA, Peek LA, Brennan M.
  Title
Overexpression, purification, and stereochemical studies of the recombinant (S)-adenosyl-L-methionine: delta 24(25)- to delta 24(28)-sterol methyl transferase enzyme from Saccharomyces cerevisiae.
  Journal
Arch Biochem Biophys 353:297-311 (1998)
DOI:10.1006/abbi.1998.0665
Other DBs
ExplorEnz - The Enzyme Database: 2.1.1.41
IUBMB Enzyme Nomenclature: 2.1.1.41
ExPASy - ENZYME nomenclature database: 2.1.1.41
BRENDA, the Enzyme Database: 2.1.1.41
CAS: 37257-07-1

DBGET integrated database retrieval system